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The map label for this gene is trmE [H]

Identifier: 209554346

GI number: 209554346

Start: 18420

End: 19736

Strand: Reverse

Name: trmE [H]

Synonym: UUR10_0018

Alternate gene names: 209554346

Gene position: 19736-18420 (Counterclockwise)

Preceding gene: 209554429

Following gene: 209554090

Centisome position: 2.26

GC content: 26.04

Gene sequence:

>1317_bases
ATGTCAACGATTGTCGCTTTAGCAACAGCTCCTATGAATTGTGCTATTCATATTATTCGAATTAGTGGTCCACAAGCTTT
TGAAATGATTAATAAAATTTCCACAACAAAAATCAAAAAAGAAACTTTTAAAATTTGGTACACAACACTAAAAGATAATG
ATCAAGTTTTGGATGAAGTTTTAGTGAATACTTTTGTTGGACCTAAAACATTCACTGGTGAAGATTTAGTTGAAATTAAT
TGTCATGGTGGAGTAATTGTTGCTAATTTAATTATTAAAATATTAATTAAGTATGGTTGTCAGCCAGCTCAAAGGGGTGA
ATTTTCACGTCGTGCTTTATTAAATAAAAAGATGGATCTATCAAAAATTGAAGCTATTAATAATTTAGTTAATGCGAAAA
ATGAATTAAGCGTTAAAGGTGTTATTGGGGCTTTATTAGGACGTGTTTCACAATCAATTAGTGATTTTAAACATGAATTA
TTTATGATAATTGGTCAAATCGAAGTTAATATTGATTATCCTGAATATGATGATGTTGAACAAGTTGATGCAATTAATTT
AAAACAACGTTTGTTAGTGCTAAACGAAAAGATTAAAAAGATTATTGATCAATCAAAAAAATTCTTACCAATTAATAAAG
GGATTAAAGTATTAATAATTGGTAAACCAAATGTCGGTAAATCAACTTTGTTAAATGCTTTATGTAATGAACAAAAAGCT
ATTGTCACAGATATTCCTGGAACAACACGTGATGTGATTGAATCATCAATTAATATCGATAATATTACTTTAAATATCTT
AGACACTGCTGGTATTCATTCAACAAATGATTTTGTTGAAAACTTAGGGATCAATAAAGCTAAAGAATTAATTAATAAAG
TTGATTTAGTTTTATATTTAGTGCCAGCAAACAATCAACAAGATTTAGAATTATATGATTTAATTAAAGATCAAAAACAC
TTATTAGTTTATACAAAAAAAGATTTAATTGATCAATATAGTGATGATCAAATCTATATTAATGCAAAAGATAATGATAT
TCAAGCATTAATTGATAAAATCAAAGAACTTTTTTATGTTCAAGAATTTGATAATGCAAACATTGATGTTTTACAATCAC
AACGTCAAATAGGTATTTTAGAAAACGTTAATTATCTAATTGATAATGCAATTACTAATTTAGAAAAAGGGGACACAGTT
GATTTAGTTGTTGCTGATTTAGAATTTTGTAATTTACGTTTAAATGAACTATTAGGAATAGGTAGTGAATACGATTTCTT
AGATGATTTATTTAAAAATTTTTGCGTAGGTAAATAG

Upstream 100 bases:

>100_bases
TTTTAGATTTAATGCGAGCAAATTTAAAAATCAATATTAACAAAAATAGTTTATTTACAATTATTTATACAATTATTATT
GAAAATAGAGGTGATTAATA

Downstream 100 bases:

>100_bases
TATGTTAGAAAAAATAAAAATTATTGACACTCACACACATCCCAATATTGAACCACTAATTGATGAATTTGATGATATTA
TTGCTGAATGCTATAAACAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSTIVALATAPMNCAIHIIRISGPQAFEMINKISTTKIKKETFKIWYTTLKDNDQVLDEVLVNTFVGPKTFTGEDLVEIN
CHGGVIVANLIIKILIKYGCQPAQRGEFSRRALLNKKMDLSKIEAINNLVNAKNELSVKGVIGALLGRVSQSISDFKHEL
FMIIGQIEVNIDYPEYDDVEQVDAINLKQRLLVLNEKIKKIIDQSKKFLPINKGIKVLIIGKPNVGKSTLLNALCNEQKA
IVTDIPGTTRDVIESSINIDNITLNILDTAGIHSTNDFVENLGINKAKELINKVDLVLYLVPANNQQDLELYDLIKDQKH
LLVYTKKDLIDQYSDDQIYINAKDNDIQALIDKIKELFYVQEFDNANIDVLQSQRQIGILENVNYLIDNAITNLEKGDTV
DLVVADLEFCNLRLNELLGIGSEYDFLDDLFKNFCVGK

Sequences:

>Translated_438_residues
MSTIVALATAPMNCAIHIIRISGPQAFEMINKISTTKIKKETFKIWYTTLKDNDQVLDEVLVNTFVGPKTFTGEDLVEIN
CHGGVIVANLIIKILIKYGCQPAQRGEFSRRALLNKKMDLSKIEAINNLVNAKNELSVKGVIGALLGRVSQSISDFKHEL
FMIIGQIEVNIDYPEYDDVEQVDAINLKQRLLVLNEKIKKIIDQSKKFLPINKGIKVLIIGKPNVGKSTLLNALCNEQKA
IVTDIPGTTRDVIESSINIDNITLNILDTAGIHSTNDFVENLGINKAKELINKVDLVLYLVPANNQQDLELYDLIKDQKH
LLVYTKKDLIDQYSDDQIYINAKDNDIQALIDKIKELFYVQEFDNANIDVLQSQRQIGILENVNYLIDNAITNLEKGDTV
DLVVADLEFCNLRLNELLGIGSEYDFLDDLFKNFCVGK
>Mature_437_residues
STIVALATAPMNCAIHIIRISGPQAFEMINKISTTKIKKETFKIWYTTLKDNDQVLDEVLVNTFVGPKTFTGEDLVEINC
HGGVIVANLIIKILIKYGCQPAQRGEFSRRALLNKKMDLSKIEAINNLVNAKNELSVKGVIGALLGRVSQSISDFKHELF
MIIGQIEVNIDYPEYDDVEQVDAINLKQRLLVLNEKIKKIIDQSKKFLPINKGIKVLIIGKPNVGKSTLLNALCNEQKAI
VTDIPGTTRDVIESSINIDNITLNILDTAGIHSTNDFVENLGINKAKELINKVDLVLYLVPANNQQDLELYDLIKDQKHL
LVYTKKDLIDQYSDDQIYINAKDNDIQALIDKIKELFYVQEFDNANIDVLQSQRQIGILENVNYLIDNAITNLEKGDTVD
LVVADLEFCNLRLNELLGIGSEYDFLDDLFKNFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082993, Length=451, Percent_Identity=27.4944567627494, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=26.4516129032258, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=24.8, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI2367268, Length=458, Percent_Identity=31.8777292576419, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87082120, Length=139, Percent_Identity=33.8129496402878, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=459, Percent_Identity=30.2832244008715, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=29.0060851926978, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45550813, Length=477, Percent_Identity=27.8825995807128, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49255; Mature: 49123

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIVALATAPMNCAIHIIRISGPQAFEMINKISTTKIKKETFKIWYTTLKDNDQVLDEV
CCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHH
LVNTFVGPKTFTGEDLVEINCHGGVIVANLIIKILIKYGCQPAQRGEFSRRALLNKKMDL
HHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCH
SKIEAINNLVNAKNELSVKGVIGALLGRVSQSISDFKHELFMIIGQIEVNIDYPEYDDVE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCH
QVDAINLKQRLLVLNEKIKKIIDQSKKFLPINKGIKVLIIGKPNVGKSTLLNALCNEQKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCE
IVTDIPGTTRDVIESSINIDNITLNILDTAGIHSTNDFVENLGINKAKELINKVDLVLYL
EEECCCCCHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHEEEEEEE
VPANNQQDLELYDLIKDQKHLLVYTKKDLIDQYSDDQIYINAKDNDIQALIDKIKELFYV
EECCCCCCCHHHHHHCCCCEEEEEECHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
QEFDNANIDVLQSQRQIGILENVNYLIDNAITNLEKGDTVDLVVADLEFCNLRLNELLGI
HHCCCCCHHHECCCHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHCC
GSEYDFLDDLFKNFCVGK
CCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
STIVALATAPMNCAIHIIRISGPQAFEMINKISTTKIKKETFKIWYTTLKDNDQVLDEV
CCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHH
LVNTFVGPKTFTGEDLVEINCHGGVIVANLIIKILIKYGCQPAQRGEFSRRALLNKKMDL
HHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCH
SKIEAINNLVNAKNELSVKGVIGALLGRVSQSISDFKHELFMIIGQIEVNIDYPEYDDVE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCH
QVDAINLKQRLLVLNEKIKKIIDQSKKFLPINKGIKVLIIGKPNVGKSTLLNALCNEQKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCE
IVTDIPGTTRDVIESSINIDNITLNILDTAGIHSTNDFVENLGINKAKELINKVDLVLYL
EEECCCCCHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHEEEEEEE
VPANNQQDLELYDLIKDQKHLLVYTKKDLIDQYSDDQIYINAKDNDIQALIDKIKELFYV
EECCCCCCCHHHHHHCCCCEEEEEECHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
QEFDNANIDVLQSQRQIGILENVNYLIDNAITNLEKGDTVDLVVADLEFCNLRLNELLGI
HHCCCCCHHHECCCHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHCC
GSEYDFLDDLFKNFCVGK
CCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA