BacMap

Definition	Rhizobium leguminosarum bv. trifolii WSM2304 chromosome, complete genome.
Accession	NC_011369
Length	4,537,948

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The map label for this gene is fadB1 [H]

Identifier: 209551936

GI number: 209551936

Start: 4536482

End: 4537255

Strand: Direct

Name: fadB1 [H]

Synonym: Rleg2_4369

Alternate gene names: 209551936

Gene position: 4536482-4537255 (Clockwise)

Preceding gene: 209551935

Following gene: 209551937

Centisome position: 99.97

GC content: 63.82

Gene sequence:

>774_bases
ATGGCCTATGAGACCCTGATCGTCGAAACCCGAGGCAATGTCGGCCTCGTCACGCTGAACCGACCGCAGGCGCTGAACGC
GCTGAATTCCACCGTGCTGAAGGAATTGAAGGAGGCCTATGCCGCCTTCCACGCCGACGAGGCCGTCGGCGCGATCGTCA
TCACCGGTTCCGAGCGCGCCTTTGCGGCCGGCGCCGATATCAAGGAGATGCAGGCGCTTCAATTCGCCGATATCTATAAG
AGCGATTTCATCAGCGGCTGGGACGAGGTCGCCAAGGCCCGCAAGCCGGTGATCGCCGCCGTCAGCGGCTTTGCGCTCGG
CGGCGGCTGCGAGCTGGCGATGATGTGCGATTTCATCATCGCTTCGGAGACGGCGAAGTTCGGCCAGCCGGAAATCACCC
TCGGCGTCATTCCCGGCATGGGCGGCTCGCAGCGGCTGACGCGCGCCGTCGGCAAGGCGAAAGCGATGGATCTCATCCTG
ACCGGCCGAATGATGGATGCGGCGGAAGCCGAACGCTCGGGACTCGTATCGCGTGTGGTGGCGCCCGAGCGTCTGCTCGA
GGAGGCTCTTGCCGCGGCGGAAAAGATTGCCTCGCTTTCGCAGCCCTCGGTGATGATGGCCAAGGAGGCGGTCAACCGCG
CCTTCGAGACGACGCTGGAGGAGGGGCTCCGCTTCGAACGCCGGCTGTTTCACAGTCTCTTTGCCACCGACGACCAGAAG
GAAGGCATGGCGGCCTTCGTCGAGAAGCGCAAACCGGCTTTTAAGCACCGTTGA

Upstream 100 bases:

>100_bases
CCGCACGCCCGGCTGCGGCGGTACGGTCGCCCGCATCGTCCAGGCGGGCCGCTCCACCTTCTATTGCGCCACCTGCCAGA
AGTAAGAACGGGAGAACAGC

Downstream 100 bases:

>100_bases
ACGCTTTTACGCGGTTCCGGCCGCAAAGCTTGAAAATCCGCGTTGACGTGGGCCGGCTTTAGAGTTATATGCCCGCCCAC
GGTTCGGAAAGCCGGTTTGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MAYETLIVETRGNVGLVTLNRPQALNALNSTVLKELKEAYAAFHADEAVGAIVITGSERAFAAGADIKEMQALQFADIYK
SDFISGWDEVAKARKPVIAAVSGFALGGGCELAMMCDFIIASETAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLIL
TGRMMDAAEAERSGLVSRVVAPERLLEEALAAAEKIASLSQPSVMMAKEAVNRAFETTLEEGLRFERRLFHSLFATDDQK
EGMAAFVEKRKPAFKHR

Sequences:

>Translated_257_residues
MAYETLIVETRGNVGLVTLNRPQALNALNSTVLKELKEAYAAFHADEAVGAIVITGSERAFAAGADIKEMQALQFADIYK
SDFISGWDEVAKARKPVIAAVSGFALGGGCELAMMCDFIIASETAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLIL
TGRMMDAAEAERSGLVSRVVAPERLLEEALAAAEKIASLSQPSVMMAKEAVNRAFETTLEEGLRFERRLFHSLFATDDQK
EGMAAFVEKRKPAFKHR
>Mature_256_residues
AYETLIVETRGNVGLVTLNRPQALNALNSTVLKELKEAYAAFHADEAVGAIVITGSERAFAAGADIKEMQALQFADIYKS
DFISGWDEVAKARKPVIAAVSGFALGGGCELAMMCDFIIASETAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLILT
GRMMDAAEAERSGLVSRVVAPERLLEEALAAAEKIASLSQPSVMMAKEAVNRAFETTLEEGLRFERRLFHSLFATDDQKE
GMAAFVEKRKPAFKHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=59.3023255813954, Blast_Score=311, Evalue=4e-85,
Organism=Homo sapiens, GI68989263, Length=204, Percent_Identity=36.2745098039216, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=37.4015748031496, Blast_Score=121, Evalue=5e-28,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=31.3008130081301, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI20127408, Length=222, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=32.7067669172932, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=34.1463414634146, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI37594471, Length=236, Percent_Identity=30.0847457627119, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI37594469, Length=211, Percent_Identity=31.7535545023697, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4757968, Length=265, Percent_Identity=25.2830188679245, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI49355787, Length=265, Percent_Identity=25.2830188679245, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI25453481, Length=264, Percent_Identity=25.7575757575758, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI51538573, Length=264, Percent_Identity=25.7575757575758, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI213417737, Length=237, Percent_Identity=26.1603375527426, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=237, Percent_Identity=26.1603375527426, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221136756, Length=235, Percent_Identity=25.531914893617, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI221136753, Length=235, Percent_Identity=25.531914893617, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4757966, Length=224, Percent_Identity=27.6785714285714, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI51538581, Length=224, Percent_Identity=27.6785714285714, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI116235487, Length=234, Percent_Identity=24.7863247863248, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI221307494, Length=235, Percent_Identity=25.531914893617, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=226, Percent_Identity=26.9911504424779, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260275230, Length=226, Percent_Identity=26.9911504424779, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260274832, Length=226, Percent_Identity=26.9911504424779, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI261878539, Length=113, Percent_Identity=38.0530973451327, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI157694520, Length=200, Percent_Identity=26.5, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI62530384, Length=258, Percent_Identity=23.2558139534884, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=46.031746031746, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=35.361216730038, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1788597, Length=245, Percent_Identity=31.8367346938775, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=32.6848249027237, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=30.1526717557252, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790281, Length=187, Percent_Identity=32.620320855615, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=33.0049261083744, Blast_Score=95, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=57.2, Blast_Score=290, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=52.0661157024793, Blast_Score=263, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=34.5454545454545, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=251, Percent_Identity=32.6693227091633, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17558304, Length=230, Percent_Identity=31.304347826087, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17540306, Length=252, Percent_Identity=30.952380952381, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=225, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=225, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=225, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=271, Percent_Identity=29.520295202952, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=28.5185185185185, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25144157, Length=225, Percent_Identity=30.6666666666667, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144160, Length=225, Percent_Identity=30.6666666666667, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=205, Percent_Identity=29.2682926829268, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=24.609375, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=188, Percent_Identity=29.2553191489362, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=57.1984435797665, Blast_Score=304, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=57.1984435797665, Blast_Score=304, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=35.15625, Blast_Score=137, Evalue=6e-33,
Organism=Drosophila melanogaster, GI21357171, Length=259, Percent_Identity=31.6602316602317, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24650670, Length=233, Percent_Identity=34.3347639484979, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19920382, Length=272, Percent_Identity=33.8235294117647, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=26.615969581749, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24654903, Length=217, Percent_Identity=27.1889400921659, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19921000, Length=220, Percent_Identity=34.0909090909091, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583077, Length=220, Percent_Identity=34.0909090909091, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24583079, Length=220, Percent_Identity=34.0909090909091, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571730, Length=231, Percent_Identity=29.8701298701299, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI28571729, Length=231, Percent_Identity=29.8701298701299, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24583165, Length=174, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921018, Length=188, Percent_Identity=26.063829787234, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27717; Mature: 27586

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYETLIVETRGNVGLVTLNRPQALNALNSTVLKELKEAYAAFHADEAVGAIVITGSERA
CCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
FAAGADIKEMQALQFADIYKSDFISGWDEVAKARKPVIAAVSGFALGGGCELAMMCDFII
EECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEHHHCEEECCCCHHHHHHHHHH
ASETAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLILTGRMMDAAEAERSGLVSRVV
HHCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
APERLLEEALAAAEKIASLSQPSVMMAKEAVNRAFETTLEEGLRFERRLFHSLFATDDQK
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMAAFVEKRKPAFKHR
CHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYETLIVETRGNVGLVTLNRPQALNALNSTVLKELKEAYAAFHADEAVGAIVITGSERA
CCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
FAAGADIKEMQALQFADIYKSDFISGWDEVAKARKPVIAAVSGFALGGGCELAMMCDFII
EECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEHHHCEEECCCCHHHHHHHHHH
ASETAKFGQPEITLGVIPGMGGSQRLTRAVGKAKAMDLILTGRMMDAAEAERSGLVSRVV
HHCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
APERLLEEALAAAEKIASLSQPSVMMAKEAVNRAFETTLEEGLRFERRLFHSLFATDDQK
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMAAFVEKRKPAFKHR
CHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]