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The map label for this gene is yafC [C]

Identifier: 209551915

GI number: 209551915

Start: 4513281

End: 4514174

Strand: Reverse

Name: yafC [C]

Synonym: Rleg2_4348

Alternate gene names: 209551915

Gene position: 4514174-4513281 (Counterclockwise)

Preceding gene: 209551917

Following gene: 209551913

Centisome position: 99.48

GC content: 64.43

Gene sequence:

>894_bases
ATGGCGCTTTTCAACCTCAACGACCTGCATCTCTTTGTGCAGGCGGTCGACAGCGGGGGTTTCACGGCGGCGGCAAGACA
TCTCGGCATCCCCAAATCGACGGTCAGCAAGCGGGTTGCGGAACTGGAAGGCCGGCTTGGCGTTCGGCTGATCCAGCGCA
CTTCACGGAGCTTCGCGCTGACCGAGCTCGGCCGGGAATTCTTTCAGCACGCCCAGGCCTCGATCATCGAGGCGGAGATG
GCGGAGGGCATCGTGCGGCGGCATCTGGCTGAACCCGCCGGCAGCGTTCGCCTGACCGCTTCGGTGCCGACAGCGCAGTT
CACCCTGACCGAGCATCTGCCGGCATTGGCGGCGCGCTATCCGAAGCTTCGGCTGTCCGTGCATGTGACCGACCGTTTCG
TCGACATCGTCCAGGAAGGCTTCGACATCGCACTACGCAGCCACCGCGCGCCGCTGCCGGATTCTGCGTTGGTACAGCGC
AAACTTGCCAGCCATCCCTTCCTCATTCTCGCGTCGCCCGAATACATCCGTGCCCATGGGCAACCGCGCAGGCCGGAAGA
ACTGACGCAACACGCGACGATCATGACCAGCCTGACCGACAATCAATGGTGGCTCTATTGCGATAACGGCGACGAGTCGC
TGGTCACACTACAGCCCATCATGGCGGCGGACGAGCCCTTTGTGCTGATGGAAGCGGCAGCGGCCGGGCTCGGCATCACC
TGCCTGCCGACATCGGTCTGCCGGAAGGCGCTGGCGACCAAGCGGCTGGTGCGGCTGTTGCCGGACTGGACCGCCGGTAA
CATCGAAACGACGATCCTGATGCCGCACCGACGTGGCCAACTGCCGGCGGTGCGCGCGGTCGTCGATTTTTTGGCGGAAC
GGCTCGCCGGCTAG

Upstream 100 bases:

>100_bases
CAGAATTGAACCGTGTGGACGTGATCAATGTGCAGATGTGGACGATCCTGCGGTAGAGGTTATAAATGCACCCCAATGTC
TACGCTAATGGAAGATTATC

Downstream 100 bases:

>100_bases
AATCGCCTCGCGAGAATTGCTTTGGGCATCACGCCGCCTTGGCGGCGTGATATTCCTTGATGGCGACCATCTTGATCGCC
GGATGGCGTTCGGACTCGTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MALFNLNDLHLFVQAVDSGGFTAAARHLGIPKSTVSKRVAELEGRLGVRLIQRTSRSFALTELGREFFQHAQASIIEAEM
AEGIVRRHLAEPAGSVRLTASVPTAQFTLTEHLPALAARYPKLRLSVHVTDRFVDIVQEGFDIALRSHRAPLPDSALVQR
KLASHPFLILASPEYIRAHGQPRRPEELTQHATIMTSLTDNQWWLYCDNGDESLVTLQPIMAADEPFVLMEAAAAGLGIT
CLPTSVCRKALATKRLVRLLPDWTAGNIETTILMPHRRGQLPAVRAVVDFLAERLAG

Sequences:

>Translated_297_residues
MALFNLNDLHLFVQAVDSGGFTAAARHLGIPKSTVSKRVAELEGRLGVRLIQRTSRSFALTELGREFFQHAQASIIEAEM
AEGIVRRHLAEPAGSVRLTASVPTAQFTLTEHLPALAARYPKLRLSVHVTDRFVDIVQEGFDIALRSHRAPLPDSALVQR
KLASHPFLILASPEYIRAHGQPRRPEELTQHATIMTSLTDNQWWLYCDNGDESLVTLQPIMAADEPFVLMEAAAAGLGIT
CLPTSVCRKALATKRLVRLLPDWTAGNIETTILMPHRRGQLPAVRAVVDFLAERLAG
>Mature_296_residues
ALFNLNDLHLFVQAVDSGGFTAAARHLGIPKSTVSKRVAELEGRLGVRLIQRTSRSFALTELGREFFQHAQASIIEAEMA
EGIVRRHLAEPAGSVRLTASVPTAQFTLTEHLPALAARYPKLRLSVHVTDRFVDIVQEGFDIALRSHRAPLPDSALVQRK
LASHPFLILASPEYIRAHGQPRRPEELTQHATIMTSLTDNQWWLYCDNGDESLVTLQPIMAADEPFVLMEAAAAGLGITC
LPTSVCRKALATKRLVRLLPDWTAGNIETTILMPHRRGQLPAVRAVVDFLAERLAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=30.5841924398625, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.0547945205479, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1789639, Length=261, Percent_Identity=28.3524904214559, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=26.4406779661017, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=28.1786941580756, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=29.4915254237288, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787589, Length=296, Percent_Identity=26.3513513513513, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788706, Length=301, Percent_Identity=25.2491694352159, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789173, Length=252, Percent_Identity=24.2063492063492, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788748, Length=250, Percent_Identity=25.6, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1788887, Length=306, Percent_Identity=26.1437908496732, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32741; Mature: 32610

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFNLNDLHLFVQAVDSGGFTAAARHLGIPKSTVSKRVAELEGRLGVRLIQRTSRSFAL
CCEECCHHHEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TELGREFFQHAQASIIEAEMAEGIVRRHLAEPAGSVRLTASVPTAQFTLTEHLPALAARY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHHCC
PKLRLSVHVTDRFVDIVQEGFDIALRSHRAPLPDSALVQRKLASHPFLILASPEYIRAHG
CEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCHHHHCCC
QPRRPEELTQHATIMTSLTDNQWWLYCDNGDESLVTLQPIMAADEPFVLMEAAAAGLGIT
CCCCHHHHHHHHHHEEEECCCEEEEEECCCCCCEEEEEHHHCCCCCEEEEEHHHCCCCEE
CLPTSVCRKALATKRLVRLLPDWTAGNIETTILMPHRRGQLPAVRAVVDFLAERLAG
ECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALFNLNDLHLFVQAVDSGGFTAAARHLGIPKSTVSKRVAELEGRLGVRLIQRTSRSFAL
CEECCHHHEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TELGREFFQHAQASIIEAEMAEGIVRRHLAEPAGSVRLTASVPTAQFTLTEHLPALAARY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHHCC
PKLRLSVHVTDRFVDIVQEGFDIALRSHRAPLPDSALVQRKLASHPFLILASPEYIRAHG
CEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCHHHHCCC
QPRRPEELTQHATIMTSLTDNQWWLYCDNGDESLVTLQPIMAADEPFVLMEAAAAGLGIT
CCCCHHHHHHHHHHEEEECCCEEEEEECCCCCCEEEEEHHHCCCCCEEEEEHHHCCCCEE
CLPTSVCRKALATKRLVRLLPDWTAGNIETTILMPHRRGQLPAVRAVVDFLAERLAG
ECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]