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The map label for this gene is recF [H]

Identifier: 209551663

GI number: 209551663

Start: 4265727

End: 4266851

Strand: Reverse

Name: recF [H]

Synonym: Rleg2_4092

Alternate gene names: 209551663

Gene position: 4266851-4265727 (Counterclockwise)

Preceding gene: 209551665

Following gene: 209551662

Centisome position: 94.03

GC content: 66.04

Gene sequence:

>1125_bases
ATGCCGCACAAGGTTTCTCTTTCCCGCCTGAAGCTGACAGACTTCCGCAATTATGCGGCGGCGGCGCTTGACCTTGACGG
CCGGCACGCCGTGCTGACGGGAGACAACGGCGCCGGCAAGACCAATCTCATGGAGGCTGTCTCGCTGCTTTCGCCCGGCC
GGGGGCTGCGCCGCGCCGCCTATGGCGACATTACCCGCGTCGGCGCGGCCGGCGGTTTTTCGATCTTCGCGGCACTCGAC
GGCATGGAAGGCGAAGTCGAAATCGGCACCGGTATTGAAACGGGCGAGGAGACCACGGCCCGCAGGCTGCGGATCAACGG
TACCCAGGCCAAGACCGCAGACGAACTTACCGACCATCTGCGCCTTCTCTGGCTGACACCGGCGATGGACGGCCTCTTTA
CCGGCGCCTCATCCGACCGGCGGCGCTTTCTCGACCGGCTGGTGCTCTCGCTCGATCCCGCCCATGGCCGCCGCGCCAGC
GATTTCGAGCGCGCCATGCGCAGCCGCAACAAATTGCTCGATGAAGGCCGCTTCGACCCGTCGTGGCTTGCCGGCATCGA
GGAACAGATGGCGAGCCTCGGCATCGCCATGGCGCTCGCCCGGCAGGAGATGCTCGGCCTGCTGACCCGGCTGATCGAGG
AAACGCGCGAGACGTCACCCTTCCCTTCGGCATCGCTGCAGCTTTCCGGCTTCATGGACGGTCAATTTACGCGCCCCTCG
GTCGATCTCGAGGACGAATATGCGGCCATGCTTTCAGAGAGCCGCTACCGCGACGCCGGCGCGGGGCGCACGCTTGACGG
GCCGCATCGGGCCGATCTCATCGTGCACCACCGGGAAAAGGCGATGGAGGCGGAACGCTGCTCCACCGGCGAGCAGAAAG
CGCTGCTTGTCGGTCTGGTGCTTGCGCATGCGCGTCTCGTCGGCAATCTCACCGGCCACGCGCCGATCCTGCTGCTCGAC
GAGATCGCCGCCCATCTTGACGAGGGCCGCCGCGCCGCGCTGTTCGACCTCATCGACGGGCTGGGCGGCCAGGCCTTCAT
GACCGGAACCGATCGCGCCATGTTTTCGGCGTTGGGCGACAGGGCGCAAGTCTTCACGGTGGCCGACGGAAAGATATTCG
AATGA

Upstream 100 bases:

>100_bases
CACCGAGGGCAGCACCAATGATCCACGGCAGCGAAGTTGCGAGCCTTGCCATCAACGCCGCTTGGGCTTAGCTGTCACGC
TTCACGATTCCGGACATTCG

Downstream 100 bases:

>100_bases
CGGAGGCGACTTTTCCCGGTATCGAGCGCCATGATAAGATCGGCGCCATGGAACCGCTCAGCCCCGACGAAATCGCCCGT
TATCACCGTCACATCCTGCT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MPHKVSLSRLKLTDFRNYAAAALDLDGRHAVLTGDNGAGKTNLMEAVSLLSPGRGLRRAAYGDITRVGAAGGFSIFAALD
GMEGEVEIGTGIETGEETTARRLRINGTQAKTADELTDHLRLLWLTPAMDGLFTGASSDRRRFLDRLVLSLDPAHGRRAS
DFERAMRSRNKLLDEGRFDPSWLAGIEEQMASLGIAMALARQEMLGLLTRLIEETRETSPFPSASLQLSGFMDGQFTRPS
VDLEDEYAAMLSESRYRDAGAGRTLDGPHRADLIVHHREKAMEAERCSTGEQKALLVGLVLAHARLVGNLTGHAPILLLD
EIAAHLDEGRRAALFDLIDGLGGQAFMTGTDRAMFSALGDRAQVFTVADGKIFE

Sequences:

>Translated_374_residues
MPHKVSLSRLKLTDFRNYAAAALDLDGRHAVLTGDNGAGKTNLMEAVSLLSPGRGLRRAAYGDITRVGAAGGFSIFAALD
GMEGEVEIGTGIETGEETTARRLRINGTQAKTADELTDHLRLLWLTPAMDGLFTGASSDRRRFLDRLVLSLDPAHGRRAS
DFERAMRSRNKLLDEGRFDPSWLAGIEEQMASLGIAMALARQEMLGLLTRLIEETRETSPFPSASLQLSGFMDGQFTRPS
VDLEDEYAAMLSESRYRDAGAGRTLDGPHRADLIVHHREKAMEAERCSTGEQKALLVGLVLAHARLVGNLTGHAPILLLD
EIAAHLDEGRRAALFDLIDGLGGQAFMTGTDRAMFSALGDRAQVFTVADGKIFE
>Mature_373_residues
PHKVSLSRLKLTDFRNYAAAALDLDGRHAVLTGDNGAGKTNLMEAVSLLSPGRGLRRAAYGDITRVGAAGGFSIFAALDG
MEGEVEIGTGIETGEETTARRLRINGTQAKTADELTDHLRLLWLTPAMDGLFTGASSDRRRFLDRLVLSLDPAHGRRASD
FERAMRSRNKLLDEGRFDPSWLAGIEEQMASLGIAMALARQEMLGLLTRLIEETRETSPFPSASLQLSGFMDGQFTRPSV
DLEDEYAAMLSESRYRDAGAGRTLDGPHRADLIVHHREKAMEAERCSTGEQKALLVGLVLAHARLVGNLTGHAPILLLDE
IAAHLDEGRRAALFDLIDGLGGQAFMTGTDRAMFSALGDRAQVFTVADGKIFE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=371, Percent_Identity=28.8409703504043, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40591; Mature: 40459

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHKVSLSRLKLTDFRNYAAAALDLDGRHAVLTGDNGAGKTNLMEAVSLLSPGRGLRRAA
CCCCCCHHHEEHHHHHHHHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHH
YGDITRVGAAGGFSIFAALDGMEGEVEIGTGIETGEETTARRLRINGTQAKTADELTDHL
HCCHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCHHHHHEEEECCCCCCCHHHHHHHH
RLLWLTPAMDGLFTGASSDRRRFLDRLVLSLDPAHGRRASDFERAMRSRNKLLDEGRFDP
EEEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCH
SWLAGIEEQMASLGIAMALARQEMLGLLTRLIEETRETSPFPSASLQLSGFMDGQFTRPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
VDLEDEYAAMLSESRYRDAGAGRTLDGPHRADLIVHHREKAMEAERCSTGEQKALLVGLV
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEECHHHHHHHHHCCCCCHHHHHHHHH
LAHARLVGNLTGHAPILLLDEIAAHLDEGRRAALFDLIDGLGGQAFMTGTDRAMFSALGD
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHCC
RAQVFTVADGKIFE
CCEEEEECCCCCCC
>Mature Secondary Structure 
PHKVSLSRLKLTDFRNYAAAALDLDGRHAVLTGDNGAGKTNLMEAVSLLSPGRGLRRAA
CCCCCHHHEEHHHHHHHHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHH
YGDITRVGAAGGFSIFAALDGMEGEVEIGTGIETGEETTARRLRINGTQAKTADELTDHL
HCCHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCHHHHHEEEECCCCCCCHHHHHHHH
RLLWLTPAMDGLFTGASSDRRRFLDRLVLSLDPAHGRRASDFERAMRSRNKLLDEGRFDP
EEEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCH
SWLAGIEEQMASLGIAMALARQEMLGLLTRLIEETRETSPFPSASLQLSGFMDGQFTRPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
VDLEDEYAAMLSESRYRDAGAGRTLDGPHRADLIVHHREKAMEAERCSTGEQKALLVGLV
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEECHHHHHHHHHCCCCCHHHHHHHHH
LAHARLVGNLTGHAPILLLDEIAAHLDEGRRAALFDLIDGLGGQAFMTGTDRAMFSALGD
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHCC
RAQVFTVADGKIFE
CCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA