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The map label for this gene is truB [H]

Identifier: 209551636

GI number: 209551636

Start: 4239720

End: 4240652

Strand: Reverse

Name: truB [H]

Synonym: Rleg2_4065

Alternate gene names: 209551636

Gene position: 4240652-4239720 (Counterclockwise)

Preceding gene: 209551637

Following gene: 209551635

Centisome position: 93.45

GC content: 63.45

Gene sequence:

>933_bases
ATGTCCAAACCACGCAAACCCAAAGGCAGGCCGATTTCCGGCTGGCTGATCCTCGACAAACCGGTGGATTTCGGCTCGAC
GGAAGCCGTTTCCAAGATCAAGTGGCTCTTTAAGGCGCAGAAGGCCGGTCATGCCGGCACGCTCGATCCGCTCGCCTCCG
GCATGCTGCCGATCGCGCTCGGCGATGCGACGAAGACCGTTCCCTATGTGATGGACGGCCGGAAGATCTATGAATTCACG
GTCAGCTGGGGCGAAGAGAGGGCGACAGACGATCTTGAAGGCGAAGTGACCGCAAGCTCGGACAGCCGTCCCAGCGAACA
GCAGATCCGCGATATCCTGCCCGGATATATCGGCACCATCAGCCAGGTGCCGCCGCAGTTTTCGGCGATCAAGATATCGG
GTGAACGCGCCTATGATCTGGCGCGCGAAGGCGAAACGGTCGAAATCCCCTCGCGCGAAGTCGATATCTTCCGGTTGACG
CTGCTCGCCTGCCCGGATGCCAATAGCGCGCATTTCGAAGTGGAATGCGGCAAGGGCACCTATGTCCGGGCGCTCGCCCG
CGATTTCGGCCGCGAGCTCGGCTGTTACGGCCACGTATCGGGTCTCAGGCGCACCTTCGTCGCGCCCTTTGCGGAAGAGG
CGATGGTGCCGCTCGCGGACCTCGTGGCACTGGAGGCGATCGAGGATATGGACGAGCGGCTTGCCGCCCTCGACGCGCTG
CTGATCGACACCTGCGAGGCGCTCTCAGCCCTGCCCCACCTCGTCATCAACGACGACCAGGCGCACCGGCTGAAGATGGG
CAACCCGATCCTCGTGCGCGGCCGCGATGCGCCGATTGCCGAAAGCGAAGCCTATGCCACGGCCCGCGGCAGGCTGATCG
CGATCGGTGAGATCGGCCAGGGCGAGTTTCGCCCGAAGCGGGTTTTTGCCTGA

Upstream 100 bases:

>100_bases
AGCTTCGACAACTACAAGAAAATCGACGAGCTGCTGCGTTCGCCCGAGGTGAGCCGCGATCTCGACGGCGATAACGACGA
ACAATAAACAGAAGAGACGC

Downstream 100 bases:

>100_bases
ACCTATGCAGCTGACGATTGTCAGGCAGTAGCGATGCGATATCTTCCTTTCGGGCCATAACGGCCCGAGGGGCCATAACT
GCCCGACAAGGGGGATGATG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSKPRKPKGRPISGWLILDKPVDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIALGDATKTVPYVMDGRKIYEFT
VSWGEERATDDLEGEVTASSDSRPSEQQIRDILPGYIGTISQVPPQFSAIKISGERAYDLAREGETVEIPSREVDIFRLT
LLACPDANSAHFEVECGKGTYVRALARDFGRELGCYGHVSGLRRTFVAPFAEEAMVPLADLVALEAIEDMDERLAALDAL
LIDTCEALSALPHLVINDDQAHRLKMGNPILVRGRDAPIAESEAYATARGRLIAIGEIGQGEFRPKRVFA

Sequences:

>Translated_310_residues
MSKPRKPKGRPISGWLILDKPVDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIALGDATKTVPYVMDGRKIYEFT
VSWGEERATDDLEGEVTASSDSRPSEQQIRDILPGYIGTISQVPPQFSAIKISGERAYDLAREGETVEIPSREVDIFRLT
LLACPDANSAHFEVECGKGTYVRALARDFGRELGCYGHVSGLRRTFVAPFAEEAMVPLADLVALEAIEDMDERLAALDAL
LIDTCEALSALPHLVINDDQAHRLKMGNPILVRGRDAPIAESEAYATARGRLIAIGEIGQGEFRPKRVFA
>Mature_309_residues
SKPRKPKGRPISGWLILDKPVDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIALGDATKTVPYVMDGRKIYEFTV
SWGEERATDDLEGEVTASSDSRPSEQQIRDILPGYIGTISQVPPQFSAIKISGERAYDLAREGETVEIPSREVDIFRLTL
LACPDANSAHFEVECGKGTYVRALARDFGRELGCYGHVSGLRRTFVAPFAEEAMVPLADLVALEAIEDMDERLAALDALL
IDTCEALSALPHLVINDDQAHRLKMGNPILVRGRDAPIAESEAYATARGRLIAIGEIGQGEFRPKRVFA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=225, Percent_Identity=32.8888888888889, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI2367200, Length=313, Percent_Identity=37.0607028753994, Blast_Score=180, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI17553978, Length=290, Percent_Identity=25.1724137931034, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=127, Percent_Identity=33.8582677165354, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323204, Length=263, Percent_Identity=26.2357414448669, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33807; Mature: 33676

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPRKPKGRPISGWLILDKPVDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIAL
CCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEE
GDATKTVPYVMDGRKIYEFTVSWGEERATDDLEGEVTASSDSRPSEQQIRDILPGYIGTI
CCCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCHHHHHH
SQVPPQFSAIKISGERAYDLAREGETVEIPSREVDIFRLTLLACPDANSAHFEVECGKGT
HHCCCCCEEEEECCCHHHHHHHCCCEEECCCCCCEEEEEEEEECCCCCCCEEEEECCCCH
YVRALARDFGRELGCYGHVSGLRRTFVAPFAEEAMVPLADLVALEAIEDMDERLAALDAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIDTCEALSALPHLVINDDQAHRLKMGNPILVRGRDAPIAESEAYATARGRLIAIGEIGQ
HHHHHHHHHHCCEEEECCCCHHEEECCCEEEEECCCCCCCCCCHHHHHCCCEEEEECCCC
GEFRPKRVFA
CCCCCCCCCC
>Mature Secondary Structure 
SKPRKPKGRPISGWLILDKPVDFGSTEAVSKIKWLFKAQKAGHAGTLDPLASGMLPIAL
CCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEE
GDATKTVPYVMDGRKIYEFTVSWGEERATDDLEGEVTASSDSRPSEQQIRDILPGYIGTI
CCCCCCCCEEECCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCHHHHHH
SQVPPQFSAIKISGERAYDLAREGETVEIPSREVDIFRLTLLACPDANSAHFEVECGKGT
HHCCCCCEEEEECCCHHHHHHHCCCEEECCCCCCEEEEEEEEECCCCCCCEEEEECCCCH
YVRALARDFGRELGCYGHVSGLRRTFVAPFAEEAMVPLADLVALEAIEDMDERLAALDAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIDTCEALSALPHLVINDDQAHRLKMGNPILVRGRDAPIAESEAYATARGRLIAIGEIGQ
HHHHHHHHHHCCEEEECCCCHHEEECCCEEEEECCCCCCCCCCHHHHHCCCEEEEECCCC
GEFRPKRVFA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA