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The map label for this gene is pnp [H]

Identifier: 209551633

GI number: 209551633

Start: 4235850

End: 4237988

Strand: Reverse

Name: pnp [H]

Synonym: Rleg2_4062

Alternate gene names: 209551633

Gene position: 4237988-4235850 (Counterclockwise)

Preceding gene: 209551634

Following gene: 209551632

Centisome position: 93.39

GC content: 61.94

Gene sequence:

>2139_bases
ATGTTTGATACACACACAGTCGAAATCGAGTGGGCCGGCCGCCCGCTGAAGCTCGAAACCGGCAAGATCGCCCGTCAGGC
CGACGGCGCGGTTCTCGCCACCTACGGCGAAACCGTCGTTCTCGCCACCGTCGTTTCGGCCAAGGCGCCGAAGCCCGGCC
AGGACTTCTTTCCGCTCACCGTCAACTACCAGGAAAAGACTTACGCAGCCGGCAAGATCCCCGGCGGCTATTTCAAGCGC
GAAGGTCGTCCGTCCGAGAAGGAAACACTGGTTTCCCGCCTGATCGACCGTCCGATCCGCCCGCTCTTTCCGGAAGGCTA
CAAGAACGACACCCAGGTCGTCGTCACCGTCGTCCAACACGATCTTGAAAACGATCCCGACGTGCTGTCGATGGTTGCCG
CTTCCGCAGCGCTCACGCTCTCCGGCGTTCCCTTCATGGGCCCGGTCGGCGGTGCGCGCGTCGGCTACATCAACGGCGAA
TACGTTCTCAACCCGCATCTCGACGAGATGGACGAGTCGAGCCTCGACCTCGTCGTCGCCGGCACCTATGACGCCGTGCT
GATGGTTGAATCCGAAGCCAAGGAACTCAACGAAGAAGTCATGCTCGGCGCCGTCATGTTCGGCCACAAGGGCTTCCAGC
CGGTTCTCGACGCGATCATCAAGCTCGCCGAAGTGGCCGCCAAGGAGCCGCGCGATTTCCAGCCGGAGGATTACTCCGAG
CTCGAAGCCGAAATGCTGAAGCATTTCGAAGCTGAATTGCGCGAAGGCTACAAGATCACCCAGAAGGCCGATCGCTACGC
AGCCGTCGACGCCGTCAAGGCAAAGGTCAAGGCTCACTTCTTCCCTGAAGGCGTTGAGCCGAAGTACACTGCTGAAGTCA
TCGGCGCCGTTTTCAAGCACCTGCAGGCGAAGATCGTCCGCTGGAACATCCTCGACACCAAGAGCCGTATCGACGGTCGT
GACCTGTCGACCGTTCGCCCGATCGTTTCGGAGGTCGGACTCCTGCCGCGCACCCACGGTTCGGCCCTGTTCACCCGCGG
TGAAACGCAGGCGATCGTCGTTGCGACGCTCGGCACCGGCGAAGACGAACAGTATGTCGACAGCCTGACGGGCATGTACA
AGGAGCGCTTCCTGCTCCATTACAACTTCCCTCCCTACTCGGTTGGCGAAACCGGCCGCATGGGCTCTCCGGGCCGTCGC
GAAATCGGCCATGGCAAGCTCGCATGGCGCGCAATCCGCCCGATGCTGCCGACGGCTGAGCAGTTCCCCTACACGCTGCG
CGTCGTTTCCGAGATCACCGAGTCGAACGGCTCGTCCTCGATGGCGACCGTCTGCGGCACCTCGCTCGCGCTGATGGATG
CCGGCGTTCCGCTGGCCAAGCCGGTTGCCGGCATCGCCATGGGTCTGATCCTGGAAGGCGATCGCTTCGCCGTCCTCTCC
GACATTCTCGGTGACGAAGACCACCTCGGCGACATGGACTTCAAGGTTGCAGGGACCGCCGACGGCATCACCTCGCTGCA
GATGGACATCAAGATCGCCGGCATCACCGAAGAGATCATGAAGGTCGCTCTTGGCCAGGCCCAGGGCGGTCGCGCCCACA
TTCTCGGCGAAATGGCGAAGGCCATCACCGAAAGCCGCGGCCAGCTCGGCGAATTCGCTCCGCGCATCGAAGTCATGAAC
ATCCCGGTCGACAAGATCCGCGAAGTCATCGGCTCCGGCGGCAAGGTCATTCGCGAAATCGTCGAAAAGACCGGCGCCAA
GATCAACATCGAGGACGACGGCACCGTCAAGATCGCCTCCTCCTCAGGCAAGGAAATCGAAGCGGCCCGCAAGTGGATCC
ACTCGATCGTCGCCGAGCCTGAAATCGGCCAGATCTACGAGGGCACTGTTGTCAAGACCGCCGACTTCGGCGCCTTCGTC
AACTTCTTCGGCGCCCGCGACGGCCTCGTCCACATCTCGCAGCTGGCTTCCGAGCGCGTTGCCAAGACGCAGGATGTCGT
CAAGGAAGGTGATAAGGTCTGGGTCAAGCTGCTCGGCTTCGACGAACGCGGCAAGGTTCGCCTGTCGATGAAGGTCGTCG
ACCAGGCCACCGGCCAGGAGATCCCGAACGAGAAGAAGAAGGAAGAAGCGGCCGAATAA

Upstream 100 bases:

>100_bases
CCCGTTGTCTTGCCCGTGATACGTCACATTATTTGCGGTCGACTGTGCGCTGCGCCTTGGTATCGGCGATGGCGCGTGCT
CCCGCATTGAAGGACAAGTC

Downstream 100 bases:

>100_bases
GCCGCGTCTCCCCGATTTCATTCCGGGCGCGGGAATCTTCCGCGCCCTTTTTGTATCCTGGTTTTGCGCATGTCGTGGCC
GCAGAACCGCTCAAACTTTT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MFDTHTVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVVQHDLENDPDVLSMVAASAALTLSGVPFMGPVGGARVGYINGE
YVLNPHLDEMDESSLDLVVAGTYDAVLMVESEAKELNEEVMLGAVMFGHKGFQPVLDAIIKLAEVAAKEPRDFQPEDYSE
LEAEMLKHFEAELREGYKITQKADRYAAVDAVKAKVKAHFFPEGVEPKYTAEVIGAVFKHLQAKIVRWNILDTKSRIDGR
DLSTVRPIVSEVGLLPRTHGSALFTRGETQAIVVATLGTGEDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPMLPTAEQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGDRFAVLS
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKVALGQAQGGRAHILGEMAKAITESRGQLGEFAPRIEVMN
IPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIASSSGKEIEAARKWIHSIVAEPEIGQIYEGTVVKTADFGAFV
NFFGARDGLVHISQLASERVAKTQDVVKEGDKVWVKLLGFDERGKVRLSMKVVDQATGQEIPNEKKKEEAAE

Sequences:

>Translated_712_residues
MFDTHTVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVVQHDLENDPDVLSMVAASAALTLSGVPFMGPVGGARVGYINGE
YVLNPHLDEMDESSLDLVVAGTYDAVLMVESEAKELNEEVMLGAVMFGHKGFQPVLDAIIKLAEVAAKEPRDFQPEDYSE
LEAEMLKHFEAELREGYKITQKADRYAAVDAVKAKVKAHFFPEGVEPKYTAEVIGAVFKHLQAKIVRWNILDTKSRIDGR
DLSTVRPIVSEVGLLPRTHGSALFTRGETQAIVVATLGTGEDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPMLPTAEQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGDRFAVLS
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKVALGQAQGGRAHILGEMAKAITESRGQLGEFAPRIEVMN
IPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIASSSGKEIEAARKWIHSIVAEPEIGQIYEGTVVKTADFGAFV
NFFGARDGLVHISQLASERVAKTQDVVKEGDKVWVKLLGFDERGKVRLSMKVVDQATGQEIPNEKKKEEAAE
>Mature_712_residues
MFDTHTVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLTVNYQEKTYAAGKIPGGYFKR
EGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVVQHDLENDPDVLSMVAASAALTLSGVPFMGPVGGARVGYINGE
YVLNPHLDEMDESSLDLVVAGTYDAVLMVESEAKELNEEVMLGAVMFGHKGFQPVLDAIIKLAEVAAKEPRDFQPEDYSE
LEAEMLKHFEAELREGYKITQKADRYAAVDAVKAKVKAHFFPEGVEPKYTAEVIGAVFKHLQAKIVRWNILDTKSRIDGR
DLSTVRPIVSEVGLLPRTHGSALFTRGETQAIVVATLGTGEDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRR
EIGHGKLAWRAIRPMLPTAEQFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGDRFAVLS
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKVALGQAQGGRAHILGEMAKAITESRGQLGEFAPRIEVMN
IPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIASSSGKEIEAARKWIHSIVAEPEIGQIYEGTVVKTADFGAFV
NFFGARDGLVHISQLASERVAKTQDVVKEGDKVWVKLLGFDERGKVRLSMKVVDQATGQEIPNEKKKEEAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=723, Percent_Identity=37.2060857538036, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI21361576, Length=90, Percent_Identity=46.6666666666667, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4826690, Length=93, Percent_Identity=49.4623655913978, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=57.2254335260116, Blast_Score=789, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=736, Percent_Identity=32.8804347826087, Blast_Score=349, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17535281, Length=84, Percent_Identity=48.8095238095238, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320850, Length=109, Percent_Identity=36.697247706422, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI281362905, Length=732, Percent_Identity=37.2950819672131, Blast_Score=441, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651641, Length=732, Percent_Identity=37.2950819672131, Blast_Score=441, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651643, Length=732, Percent_Identity=37.2950819672131, Blast_Score=441, Evalue=1e-123,
Organism=Drosophila melanogaster, GI161079377, Length=675, Percent_Identity=37.037037037037, Blast_Score=394, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77501; Mature: 77501

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDTHTVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLT
CCCCEEEEEEECCCCEEECCCCHHHHCCCCEEEECCCEEEEEEHHHCCCCCCCCCCEEEE
VNYQEKTYAAGKIPGGYFKREGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVVQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEHH
DLENDPDVLSMVAASAALTLSGVPFMGPVGGARVGYINGEYVLNPHLDEMDESSLDLVVA
CCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCEEEE
GTYDAVLMVESEAKELNEEVMLGAVMFGHKGFQPVLDAIIKLAEVAAKEPRDFQPEDYSE
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LEAEMLKHFEAELREGYKITQKADRYAAVDAVKAKVKAHFFPEGVEPKYTAEVIGAVFKH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHH
LQAKIVRWNILDTKSRIDGRDLSTVRPIVSEVGLLPRTHGSALFTRGETQAIVVATLGTG
HHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPMLPTAE
CCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHH
QFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGDRFAVLS
HCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKVALGQAQGGRAHILGEMAK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHH
AITESRGQLGEFAPRIEVMNIPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIAS
HHHHHCCCHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SSGKEIEAARKWIHSIVAEPEIGQIYEGTVVKTADFGAFVNFFGARDGLVHISQLASERV
CCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHHH
AKTQDVVKEGDKVWVKLLGFDERGKVRLSMKVVDQATGQEIPNEKKKEEAAE
HHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MFDTHTVEIEWAGRPLKLETGKIARQADGAVLATYGETVVLATVVSAKAPKPGQDFFPLT
CCCCEEEEEEECCCCEEECCCCHHHHCCCCEEEECCCEEEEEEHHHCCCCCCCCCCEEEE
VNYQEKTYAAGKIPGGYFKREGRPSEKETLVSRLIDRPIRPLFPEGYKNDTQVVVTVVQH
EECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEEHH
DLENDPDVLSMVAASAALTLSGVPFMGPVGGARVGYINGEYVLNPHLDEMDESSLDLVVA
CCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCEEEE
GTYDAVLMVESEAKELNEEVMLGAVMFGHKGFQPVLDAIIKLAEVAAKEPRDFQPEDYSE
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LEAEMLKHFEAELREGYKITQKADRYAAVDAVKAKVKAHFFPEGVEPKYTAEVIGAVFKH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHH
LQAKIVRWNILDTKSRIDGRDLSTVRPIVSEVGLLPRTHGSALFTRGETQAIVVATLGTG
HHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEECCC
EDEQYVDSLTGMYKERFLLHYNFPPYSVGETGRMGSPGRREIGHGKLAWRAIRPMLPTAE
CCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHH
QFPYTLRVVSEITESNGSSSMATVCGTSLALMDAGVPLAKPVAGIAMGLILEGDRFAVLS
HCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHEEECCCCHHHHH
DILGDEDHLGDMDFKVAGTADGITSLQMDIKIAGITEEIMKVALGQAQGGRAHILGEMAK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHH
AITESRGQLGEFAPRIEVMNIPVDKIREVIGSGGKVIREIVEKTGAKINIEDDGTVKIAS
HHHHHCCCHHHHCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEC
SSGKEIEAARKWIHSIVAEPEIGQIYEGTVVKTADFGAFVNFFGARDGLVHISQLASERV
CCCCHHHHHHHHHHHHHCCCCCCCEECCCEEEECCHHHHHHHHCCCCCHHHHHHHHHHHH
AKTQDVVKEGDKVWVKLLGFDERGKVRLSMKVVDQATGQEIPNEKKKEEAAE
HHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA