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The map label for this gene is prpE [C]

Identifier: 209551493

GI number: 209551493

Start: 4070735

End: 4071904

Strand: Reverse

Name: prpE [C]

Synonym: Rleg2_3922

Alternate gene names: 209551493

Gene position: 4071904-4070735 (Counterclockwise)

Preceding gene: 209551494

Following gene: 209551491

Centisome position: 89.73

GC content: 63.25

Gene sequence:

>1170_bases
ATGCAGAACGGCTATCATCAGGCTTATGCTGCCTGGAAACGGGATCCCGAAGCCTTCTGGCGCGAAGCCGCCGCCGACAT
CGACTGGTTTAAACCGCCGGCGCGGGTGTTTTCGCCCGAGGAAGGCGTCTATGGCCGCTGGTTCTCAGGGGCTGAAACCA
ATACCTGCCACAATTGCCTCGACCGGCATGTGACTGCCGGGCGTGGTGGCGAGATGGCGGTTATTTTCGACAGTGCGATG
ACCGGCGAGAAGCGCCGTTTCACCTACGACGAAGTCCTTGACGAAGTGAAGGCCATCGCCGCGACGCTTGTTGATCTCGG
GATCGGTCGGGGCGATCGCGTCATCCTCTATATGCCGATGGTGCCGCAGGCGGTGTTTTCGATGCTCGCCTGCGCCCGCA
TCGGTGCGGTTCACTCCGTCGTCTTCGGTGGTTTTGCCGCCAGCGAGCTTGCTGCCCGCATCGATGATTGCGGTGCGAAG
CTGGTGATCACCGCGAGCTGCGGGCTCGAGCCCGGCCGCATCGTTGCCTATAAGCCCCTGGTCGACCAGGCGCTCACGTT
GGCGCGTTCGAAGCCGGAGCGCTGTCTGGTGCTGCAGCGGCCGGAGCTTCGGGCGGATCTCGTCAGCGGCCGCGATCAGG
ATTTCGAGGCGGCGGTGGCGCAGCATCGCGGCGCCGAGATCGCCTGTGTTCCGGTCAAGGCGACCGACCCGCTCTATATC
CTTTACACCTCGGGAACCACCGGCCAGCCGAAGGGCGTCGTGCGCGACACCGGCGCGATCGAGACGAGCAGCGGGATCAT
CGGCACGGCCTTCAACGGAACCTATGGCGGCACCTTCATCGTTATGGCGTCGATCTATGATCTCGATATCACCGGTTTCA
CCCAGGGCCAGCTCGATTCAGCCCTGACCGGCGTCGAACTGGTTTTGGGTGCCATGACCGCCGCCGGCTCGGCTCTCGGC
TCGATCTCGACCCGTATCCAGCTGCAGGAAAATTTCGTCAGCGGTCTTCACGATTCGATCGACTCCGGCGTCGGCCGCCT
GGTCGATGCCGATATGGAAGAGGAATCGAGCAAGCTGTCGGCGCTGCAGACGCAGCAGCAGCTCGCCGTCCAGTCGCTGT
CGATCGCCAACAGCTCGGCGCAGAACATCCTCACCCTGTTCCGCAGCTAA

Upstream 100 bases:

>100_bases
ATTTTCGGCGCTCGCTGATACTCTGCTTTGGTCGGGCTTCCCGTTCCCCCTACTGCTGCCTAGATTGATATCGACGCTTG
CATATCCTGGGGAGGAGCAG

Downstream 100 bases:

>100_bases
TCCGCAACAAAAAACCCCGCCGGTCAGCTCATACCGGCGGGGTTTTTCATGAGATCGTCGATCTCTCAGTACTGATCGTC
GTCGTCCTCGTCCTTCGGCG

Product: flagellin domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MQNGYHQAYAAWKRDPEAFWREAAADIDWFKPPARVFSPEEGVYGRWFSGAETNTCHNCLDRHVTAGRGGEMAVIFDSAM
TGEKRRFTYDEVLDEVKAIAATLVDLGIGRGDRVILYMPMVPQAVFSMLACARIGAVHSVVFGGFAASELAARIDDCGAK
LVITASCGLEPGRIVAYKPLVDQALTLARSKPERCLVLQRPELRADLVSGRDQDFEAAVAQHRGAEIACVPVKATDPLYI
LYTSGTTGQPKGVVRDTGAIETSSGIIGTAFNGTYGGTFIVMASIYDLDITGFTQGQLDSALTGVELVLGAMTAAGSALG
SISTRIQLQENFVSGLHDSIDSGVGRLVDADMEEESSKLSALQTQQQLAVQSLSIANSSAQNILTLFRS

Sequences:

>Translated_389_residues
MQNGYHQAYAAWKRDPEAFWREAAADIDWFKPPARVFSPEEGVYGRWFSGAETNTCHNCLDRHVTAGRGGEMAVIFDSAM
TGEKRRFTYDEVLDEVKAIAATLVDLGIGRGDRVILYMPMVPQAVFSMLACARIGAVHSVVFGGFAASELAARIDDCGAK
LVITASCGLEPGRIVAYKPLVDQALTLARSKPERCLVLQRPELRADLVSGRDQDFEAAVAQHRGAEIACVPVKATDPLYI
LYTSGTTGQPKGVVRDTGAIETSSGIIGTAFNGTYGGTFIVMASIYDLDITGFTQGQLDSALTGVELVLGAMTAAGSALG
SISTRIQLQENFVSGLHDSIDSGVGRLVDADMEEESSKLSALQTQQQLAVQSLSIANSSAQNILTLFRS
>Mature_389_residues
MQNGYHQAYAAWKRDPEAFWREAAADIDWFKPPARVFSPEEGVYGRWFSGAETNTCHNCLDRHVTAGRGGEMAVIFDSAM
TGEKRRFTYDEVLDEVKAIAATLVDLGIGRGDRVILYMPMVPQAVFSMLACARIGAVHSVVFGGFAASELAARIDDCGAK
LVITASCGLEPGRIVAYKPLVDQALTLARSKPERCLVLQRPELRADLVSGRDQDFEAAVAQHRGAEIACVPVKATDPLYI
LYTSGTTGQPKGVVRDTGAIETSSGIIGTAFNGTYGGTFIVMASIYDLDITGFTQGQLDSALTGVELVLGAMTAAGSALG
SISTRIQLQENFVSGLHDSIDSGVGRLVDADMEEESSKLSALQTQQQLAVQSLSIANSSAQNILTLFRS

Specific function: Catalyzes The Synthesis Of Propionyl-CoA From Propionate And CoA. Also Converts Acetate To Acetyl-CoA But With A Lower Specific Activity (By Similarity). [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=255, Percent_Identity=49.4117647058824, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI28416953, Length=248, Percent_Identity=40.3225806451613, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI8923896, Length=275, Percent_Identity=35.6363636363636, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI253970489, Length=288, Percent_Identity=34.0277777777778, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI31982927, Length=226, Percent_Identity=26.9911504424779, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786529, Length=275, Percent_Identity=43.2727272727273, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1790505, Length=252, Percent_Identity=42.4603174603175, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71982997, Length=275, Percent_Identity=33.4545454545455, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71983001, Length=275, Percent_Identity=33.4545454545455, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319264, Length=264, Percent_Identity=36.3636363636364, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323182, Length=264, Percent_Identity=37.1212121212121, Blast_Score=159, Evalue=6e-40,
Organism=Drosophila melanogaster, GI21355225, Length=259, Percent_Identity=42.8571428571429, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24667955, Length=285, Percent_Identity=35.7894736842105, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI62472339, Length=187, Percent_Identity=41.1764705882353, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24667959, Length=154, Percent_Identity=39.6103896103896, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.17 [C]

Molecular weight: Translated: 41629; Mature: 41629

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNGYHQAYAAWKRDPEAFWREAAADIDWFKPPARVFSPEEGVYGRWFSGAETNTCHNCL
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEECCCCCCHHHHHH
DRHVTAGRGGEMAVIFDSAMTGEKRRFTYDEVLDEVKAIAATLVDLGIGRGDRVILYMPM
HHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
VPQAVFSMLACARIGAVHSVVFGGFAASELAARIDDCGAKLVITASCGLEPGRIVAYKPL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECHH
VDQALTLARSKPERCLVLQRPELRADLVSGRDQDFEAAVAQHRGAEIACVPVKATDPLYI
HHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEECCCCEEE
LYTSGTTGQPKGVVRDTGAIETSSGIIGTAFNGTYGGTFIVMASIYDLDITGFTQGQLDS
EEECCCCCCCCCCEECCCCEECCCCEEEEECCCCCCCEEEEEEEEEEEEECCCCCCHHHH
ALTGVELVLGAMTAAGSALGSISTRIQLQENFVSGLHDSIDSGVGRLVDADMEEESSKLS
HHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ALQTQQQLAVQSLSIANSSAQNILTLFRS
HHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MQNGYHQAYAAWKRDPEAFWREAAADIDWFKPPARVFSPEEGVYGRWFSGAETNTCHNCL
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEECCCCCCHHHHHH
DRHVTAGRGGEMAVIFDSAMTGEKRRFTYDEVLDEVKAIAATLVDLGIGRGDRVILYMPM
HHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
VPQAVFSMLACARIGAVHSVVFGGFAASELAARIDDCGAKLVITASCGLEPGRIVAYKPL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECHH
VDQALTLARSKPERCLVLQRPELRADLVSGRDQDFEAAVAQHRGAEIACVPVKATDPLYI
HHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEECCCCEEE
LYTSGTTGQPKGVVRDTGAIETSSGIIGTAFNGTYGGTFIVMASIYDLDITGFTQGQLDS
EEECCCCCCCCCCEECCCCEECCCCEEEEECCCCCCCEEEEEEEEEEEEECCCCCCHHHH
ALTGVELVLGAMTAAGSALGSISTRIQLQENFVSGLHDSIDSGVGRLVDADMEEESSKLS
HHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ALQTQQQLAVQSLSIANSSAQNILTLFRS
HHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 1339433 [H]