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The map label for this gene is phoA [H]

Identifier: 209551477

GI number: 209551477

Start: 4047159

End: 4048913

Strand: Reverse

Name: phoA [H]

Synonym: Rleg2_3906

Alternate gene names: 209551477

Gene position: 4048913-4047159 (Counterclockwise)

Preceding gene: 209551481

Following gene: 209551476

Centisome position: 89.22

GC content: 61.2

Gene sequence:

>1755_bases
ATGCGTACGCTTCTTGCCGCTTTTGCGGCCACCACCATCCTTGCGGGCGCCGCTCAGGCGACGACGGTCTATCCGCTCGA
TCGCGCGACGATCCTTGCCGGTTCGCCGTTCGATTTCAAGGTCGAACTCAACAAGCAGGTCAAGCCCGAAGACGTGAAGA
TCACGGTCAACGGCCAGGATTACAAGACCGTCCTCGGCGGCGAGGCGCAGTTCGTCGAGCTGGAAAAGGGCAAAGAGGAC
AAGGCCCTCGGCTCCGCGCTTCTGCTGCGCGGCCTGAAGATCTCCGCTCCGGGCGCCTATAAGGTCGAGGTCGCCGCCGG
CGACGAAACGAAGTCCGTCACCTGGAACGTTTACGAAACCGCTGGCCAGCCGAAAGCCAAGAACATCATCTTCCTGCTCG
GTGACGGTCTTTCCGTTGCCCATCGCACCGCTGCCCGCATCATGTCGAAGGGCATGACCGAAGGCAAGGCGAACGGTCGC
CTGAACATGGACGATCTCGATCGCATGGCTTTCATCGGCACCTCGTCGACGAACGCCGTCTCCACGGACTCGGCCAACAC
CATGTCGGCCTACATGACGGGCCACAAGACCGCCGTCAACGCGCTCGGCGTCTACGCGGACCGCACCCCGGCCTCGCTCG
ATGACCCGCGCGTCGAAACCTTCGCGGAAGCCGTTCGCCGGACGACCAAGAAGTCGATCGGTATCGTCGCAACCGCCGAA
GTCGAAGACGCGACGCCGGCTGCCGTCGTTTCCCATACCCGCAACCGCAACGACAAGGCCGATGTCGTCGGCATGCTGCT
CGACGTCAAGCCGGAAGTCCTGCTCGGCGGCGGGTCGGCCTATTTCCTCGGCAAAGAGGTCCCCGGCTCCAAGCGCAAGG
ACAACCAGGACTACATTAAGCTGTTCCAGGATGCCGGCTATAAGCTTGCGACCGACAAGAACGAGCTCGCCGCCAATGCG
TCGGCTGAAGGCAATCTCCTCGGTCTGTTCCACACCGGCAATATGGACGTCACGCTCGATCGCGAGTTCCTGAAGAAGGG
TACCGTCGACAAGTTCCCGAACCAGCCGGGTCTCGTCGCCATGACCAAGGTCGCGCTCGACCGTCTCTCCAAGAATCCGG
ACGGTTTCTTCCTGATGGTCGAAGGTTCCTCGATCGACAAGATGTCCCATCCGCTCGATTGGGATCGCGCCGTCTTCGAA
ACCATCGAATTCGACCAGGCGATCGGCGTTGCCCGCGAATTCCAGAAGGCCCATCCGGACACGCTGATCGTCGTTACCGG
CGACCACACCCACGGTGTATCGATCATCGGCACCGTCGATGACGAGAAGCCCGGCACGGAAATGCGCGAAAAGGTCGGCA
CCTATGCGGAAGCCGGCTTCCCAAACTACAAGGACGAAAATGGCGACGGTTATCCTGACAAGGTCGACGTCAGCCGCCGC
CTGTTCCTGAGCGCCACCAACGGTCCCGACCACTACGAGACCTTCCGTCCGAAGCTCGATGGTCCATTCGTTCCGGCTGT
CCAGAACGAAAAGAAGGAATATGTCGCCAACGAACAGTACAAGGATGTTCCCGGCGCGGTCTTCGTTCAGGGCAATCTTC
CGAAGAGCAGCGAAAGCGGCGTCCATGCGGTCGACGACGTCGTCCTGCAGTCGGCTGGTCCTGGTTCGGAAGAGTTCCAT
GGCTACATGGAACAGAGCGACGTCTATCGCGTGCTCGCCGACACCTTCGCTCTCGGCGCCAAGCAGACGAACTGA

Upstream 100 bases:

>100_bases
GCGGCGGCCGCGATGCTGCGACGTTTTGTCATGCCGCTGTCATCCTCGGCAGGTATCCCGGCCGGGTTCTCAATGTCTGC
AACGAAAACGGGGGTTTTCC

Downstream 100 bases:

>100_bases
TCCGAAGCCGATCCGGCAGGCCGCTTGCCGGCCTTGCCTGTTTCATCGATCATGGTCCCGGCCGTTCAGCGGCCGGGATT
TCATTCTTGGAGATCGCCAT

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MRTLLAAFAATTILAGAAQATTVYPLDRATILAGSPFDFKVELNKQVKPEDVKITVNGQDYKTVLGGEAQFVELEKGKED
KALGSALLLRGLKISAPGAYKVEVAAGDETKSVTWNVYETAGQPKAKNIIFLLGDGLSVAHRTAARIMSKGMTEGKANGR
LNMDDLDRMAFIGTSSTNAVSTDSANTMSAYMTGHKTAVNALGVYADRTPASLDDPRVETFAEAVRRTTKKSIGIVATAE
VEDATPAAVVSHTRNRNDKADVVGMLLDVKPEVLLGGGSAYFLGKEVPGSKRKDNQDYIKLFQDAGYKLATDKNELAANA
SAEGNLLGLFHTGNMDVTLDREFLKKGTVDKFPNQPGLVAMTKVALDRLSKNPDGFFLMVEGSSIDKMSHPLDWDRAVFE
TIEFDQAIGVAREFQKAHPDTLIVVTGDHTHGVSIIGTVDDEKPGTEMREKVGTYAEAGFPNYKDENGDGYPDKVDVSRR
LFLSATNGPDHYETFRPKLDGPFVPAVQNEKKEYVANEQYKDVPGAVFVQGNLPKSSESGVHAVDDVVLQSAGPGSEEFH
GYMEQSDVYRVLADTFALGAKQTN

Sequences:

>Translated_584_residues
MRTLLAAFAATTILAGAAQATTVYPLDRATILAGSPFDFKVELNKQVKPEDVKITVNGQDYKTVLGGEAQFVELEKGKED
KALGSALLLRGLKISAPGAYKVEVAAGDETKSVTWNVYETAGQPKAKNIIFLLGDGLSVAHRTAARIMSKGMTEGKANGR
LNMDDLDRMAFIGTSSTNAVSTDSANTMSAYMTGHKTAVNALGVYADRTPASLDDPRVETFAEAVRRTTKKSIGIVATAE
VEDATPAAVVSHTRNRNDKADVVGMLLDVKPEVLLGGGSAYFLGKEVPGSKRKDNQDYIKLFQDAGYKLATDKNELAANA
SAEGNLLGLFHTGNMDVTLDREFLKKGTVDKFPNQPGLVAMTKVALDRLSKNPDGFFLMVEGSSIDKMSHPLDWDRAVFE
TIEFDQAIGVAREFQKAHPDTLIVVTGDHTHGVSIIGTVDDEKPGTEMREKVGTYAEAGFPNYKDENGDGYPDKVDVSRR
LFLSATNGPDHYETFRPKLDGPFVPAVQNEKKEYVANEQYKDVPGAVFVQGNLPKSSESGVHAVDDVVLQSAGPGSEEFH
GYMEQSDVYRVLADTFALGAKQTN
>Mature_584_residues
MRTLLAAFAATTILAGAAQATTVYPLDRATILAGSPFDFKVELNKQVKPEDVKITVNGQDYKTVLGGEAQFVELEKGKED
KALGSALLLRGLKISAPGAYKVEVAAGDETKSVTWNVYETAGQPKAKNIIFLLGDGLSVAHRTAARIMSKGMTEGKANGR
LNMDDLDRMAFIGTSSTNAVSTDSANTMSAYMTGHKTAVNALGVYADRTPASLDDPRVETFAEAVRRTTKKSIGIVATAE
VEDATPAAVVSHTRNRNDKADVVGMLLDVKPEVLLGGGSAYFLGKEVPGSKRKDNQDYIKLFQDAGYKLATDKNELAANA
SAEGNLLGLFHTGNMDVTLDREFLKKGTVDKFPNQPGLVAMTKVALDRLSKNPDGFFLMVEGSSIDKMSHPLDWDRAVFE
TIEFDQAIGVAREFQKAHPDTLIVVTGDHTHGVSIIGTVDDEKPGTEMREKVGTYAEAGFPNYKDENGDGYPDKVDVSRR
LFLSATNGPDHYETFRPKLDGPFVPAVQNEKKEYVANEQYKDVPGAVFVQGNLPKSSESGVHAVDDVVLQSAGPGSEEFH
GYMEQSDVYRVLADTFALGAKQTN

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=495, Percent_Identity=29.0909090909091, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI94721246, Length=495, Percent_Identity=28.8888888888889, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI157266292, Length=498, Percent_Identity=28.3132530120482, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI116734717, Length=494, Percent_Identity=28.1376518218623, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI294660770, Length=489, Percent_Identity=27.6073619631902, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI294660772, Length=445, Percent_Identity=26.9662921348315, Blast_Score=104, Evalue=3e-22,
Organism=Escherichia coli, GI48994877, Length=396, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6320689, Length=334, Percent_Identity=29.3413173652695, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21358067, Length=495, Percent_Identity=30.5050505050505, Blast_Score=152, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24657835, Length=501, Percent_Identity=29.940119760479, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24657842, Length=502, Percent_Identity=30.8764940239044, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19921912, Length=499, Percent_Identity=28.8577154308617, Blast_Score=136, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21355149, Length=490, Percent_Identity=28.1632653061224, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24657827, Length=503, Percent_Identity=29.0258449304175, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355981, Length=493, Percent_Identity=28.8032454361055, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI18859923, Length=475, Percent_Identity=26.7368421052632, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21355151, Length=487, Percent_Identity=28.3367556468172, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24651554, Length=517, Percent_Identity=26.6924564796905, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI85725200, Length=350, Percent_Identity=30, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI85815833, Length=350, Percent_Identity=30, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24651556, Length=470, Percent_Identity=25.7446808510638, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585213, Length=499, Percent_Identity=25.4509018036072, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 63006; Mature: 63006

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLLAAFAATTILAGAAQATTVYPLDRATILAGSPFDFKVELNKQVKPEDVKITVNGQD
CHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCEEEEEECCCCCCCEEEEEECCCC
YKTVLGGEAQFVELEKGKEDKALGSALLLRGLKISAPGAYKVEVAAGDETKSVTWNVYET
EEEEECCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCEEEEEEEEC
AGQPKAKNIIFLLGDGLSVAHRTAARIMSKGMTEGKANGRLNMDDLDRMAFIGTSSTNAV
CCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECCCCCCE
STDSANTMSAYMTGHKTAVNALGVYADRTPASLDDPRVETFAEAVRRTTKKSIGIVATAE
ECCCCCCEEEECCCCHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEC
VEDATPAAVVSHTRNRNDKADVVGMLLDVKPEVLLGGGSAYFLGKEVPGSKRKDNQDYIK
CCCCCCHHHHHHHCCCCCHHHHHHHEECCCCCEEEECCCEEEEECCCCCCCCCCCHHHHH
LFQDAGYKLATDKNELAANASAEGNLLGLFHTGNMDVTLDREFLKKGTVDKFPNQPGLVA
HHHHCCCEEECCCHHHCCCCCCCCCEEEEEEECCEEEEECHHHHHCCCHHCCCCCCCHHH
MTKVALDRLSKNPDGFFLMVEGSSIDKMSHPLDWDRAVFETIEFDQAIGVAREFQKAHPD
HHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TLIVVTGDHTHGVSIIGTVDDEKPGTEMREKVGTYAEAGFPNYKDENGDGYPDKVDVSRR
EEEEEECCCCCCEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCEE
LFLSATNGPDHYETFRPKLDGPFVPAVQNEKKEYVANEQYKDVPGAVFVQGNLPKSSESG
EEEEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCC
VHAVDDVVLQSAGPGSEEFHGYMEQSDVYRVLADTFALGAKQTN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRTLLAAFAATTILAGAAQATTVYPLDRATILAGSPFDFKVELNKQVKPEDVKITVNGQD
CHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCEEEEEECCCCCCCEEEEEECCCC
YKTVLGGEAQFVELEKGKEDKALGSALLLRGLKISAPGAYKVEVAAGDETKSVTWNVYET
EEEEECCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCEEEEEEEEC
AGQPKAKNIIFLLGDGLSVAHRTAARIMSKGMTEGKANGRLNMDDLDRMAFIGTSSTNAV
CCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECCCCCCE
STDSANTMSAYMTGHKTAVNALGVYADRTPASLDDPRVETFAEAVRRTTKKSIGIVATAE
ECCCCCCEEEECCCCHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEC
VEDATPAAVVSHTRNRNDKADVVGMLLDVKPEVLLGGGSAYFLGKEVPGSKRKDNQDYIK
CCCCCCHHHHHHHCCCCCHHHHHHHEECCCCCEEEECCCEEEEECCCCCCCCCCCHHHHH
LFQDAGYKLATDKNELAANASAEGNLLGLFHTGNMDVTLDREFLKKGTVDKFPNQPGLVA
HHHHCCCEEECCCHHHCCCCCCCCCEEEEEEECCEEEEECHHHHHCCCHHCCCCCCCHHH
MTKVALDRLSKNPDGFFLMVEGSSIDKMSHPLDWDRAVFETIEFDQAIGVAREFQKAHPD
HHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TLIVVTGDHTHGVSIIGTVDDEKPGTEMREKVGTYAEAGFPNYKDENGDGYPDKVDVSRR
EEEEEECCCCCCEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCEE
LFLSATNGPDHYETFRPKLDGPFVPAVQNEKKEYVANEQYKDVPGAVFVQGNLPKSSESG
EEEEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCC
VHAVDDVVLQSAGPGSEEFHGYMEQSDVYRVLADTFALGAKQTN
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]