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The map label for this gene is leuB [H]

Identifier: 209551471

GI number: 209551471

Start: 4040424

End: 4041536

Strand: Direct

Name: leuB [H]

Synonym: Rleg2_3900

Alternate gene names: 209551471

Gene position: 4040424-4041536 (Clockwise)

Preceding gene: 209551469

Following gene: 209551472

Centisome position: 89.04

GC content: 63.61

Gene sequence:

>1113_bases
ATGACTGTGCGCAATCTTTTCCTGCTGCCGGGTGACGGCATCGGTCCCGAGGCCATGGGCGAGGTCCGCAAGATCATCGC
CTATATGAACGAGGCGATGGGCGCCGGTTTCGTCACCGACGAAGGCCTTGTCGGTGGCTCCGCCTATGATGCGCATGGCG
CGGCGATCTCCGAAGCCGACATGGTAAAGGCGCTTGCCGCCGATGCCGTTCTCTTCGGCGCCGTCGGCGGTCCGAAATGG
GATAGCGTTCCCTACGAAGTCCGCCCGGAAGCCGGCCTGTTGCGGCTGCGCAAGGACCTGCAGCTGTTTGCCAACCTGCG
CCCCGCCATCTGCTATCCGGCCCTTGCCTCGGCCTCGTCGCTGAAGCCGGAACTGGTCGAAGGCCTTGATATCCTGATCA
TCCGCGAGCTGACCGGCGGCGTCTATTTCGGCGAGCCGAAGGAGATCATCGATCTCGGCAATGGCCAGAAGCGCGGCATC
GACACCCAGGTCTACGACACCTACGAGATCGAGCGCATCGCCGGCGTCGCCTTCGAAATGGCCCGCACCCGGCAGAACCG
CGTCTGCTCCATGGAAAAGCGCAACGTCATGAAGTCGGGTGTGCTCTGGAACCAGGTGGTGACCGAGACGCACAAGGCGA
AATATTCCGACGTCCAGCTCGAACATATGCTGGCCGATGCCGGCGGCATGCAGCTGGTGCGCCAGCCCAAGCAGTTCGAC
GTCATCGTCACCGACAATCTCTTCGGCGACATGCTCTCCGACGTCGCCGCCATGCTGACCGGCTCGCTCGGCATGCTGCC
ATCGGCCTCGCTCGGCGCGCCTGACGGTAAGACCGGCAAGCGCAAGGCGCTCTACGAGCCGGTGCACGGCTCCGCCCCCG
ACATCGCCGGCAAGGGCATCGCCAATCCGATCGCCATGATCGCCTCCTTCGCCATGTGCCTGCGTTACTCCTTCAATCTG
GTGAAGGAAGCCGACGATCTGGAAAAGGCGATCGCCAACGTGCTCGACAAGGGCATCCGCACCGGCGACATCATGGCCGA
TGGCGCAAGGCAGGTCGGCACCGTCGAGATGGGCGATGCGATCCTTGCCGAGTTCAAGACGCTTTCCGCCTGA

Upstream 100 bases:

>100_bases
ACGACTGAGAGCGAAGCGTACCGATTCACCTCTTCTCTCGCTTGAAATGCCCATTTCCGGGGTCTAAGAAACCGCAACTT
GTTTTTCCCAGGAGGGTTTC

Downstream 100 bases:

>100_bases
TATATTTCACGTGAAACAGTATCCGGCCCTTCGCATCGCAGATGCGGAGGGCTTTTTCATCGAGCGTCGAGAAGCCGCAA
CCCACTCGGTCGAATTCTCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTVRNLFLLPGDGIGPEAMGEVRKIIAYMNEAMGAGFVTDEGLVGGSAYDAHGAAISEADMVKALAADAVLFGAVGGPKW
DSVPYEVRPEAGLLRLRKDLQLFANLRPAICYPALASASSLKPELVEGLDILIIRELTGGVYFGEPKEIIDLGNGQKRGI
DTQVYDTYEIERIAGVAFEMARTRQNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFD
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDGKTGKRKALYEPVHGSAPDIAGKGIANPIAMIASFAMCLRYSFNL
VKEADDLEKAIANVLDKGIRTGDIMADGARQVGTVEMGDAILAEFKTLSA

Sequences:

>Translated_370_residues
MTVRNLFLLPGDGIGPEAMGEVRKIIAYMNEAMGAGFVTDEGLVGGSAYDAHGAAISEADMVKALAADAVLFGAVGGPKW
DSVPYEVRPEAGLLRLRKDLQLFANLRPAICYPALASASSLKPELVEGLDILIIRELTGGVYFGEPKEIIDLGNGQKRGI
DTQVYDTYEIERIAGVAFEMARTRQNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFD
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDGKTGKRKALYEPVHGSAPDIAGKGIANPIAMIASFAMCLRYSFNL
VKEADDLEKAIANVLDKGIRTGDIMADGARQVGTVEMGDAILAEFKTLSA
>Mature_369_residues
TVRNLFLLPGDGIGPEAMGEVRKIIAYMNEAMGAGFVTDEGLVGGSAYDAHGAAISEADMVKALAADAVLFGAVGGPKWD
SVPYEVRPEAGLLRLRKDLQLFANLRPAICYPALASASSLKPELVEGLDILIIRELTGGVYFGEPKEIIDLGNGQKRGID
TQVYDTYEIERIAGVAFEMARTRQNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFDV
IVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDGKTGKRKALYEPVHGSAPDIAGKGIANPIAMIASFAMCLRYSFNLV
KEADDLEKAIANVLDKGIRTGDIMADGARQVGTVEMGDAILAEFKTLSA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=339, Percent_Identity=33.6283185840708, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI28178821, Length=325, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI28178816, Length=325, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4758582, Length=347, Percent_Identity=29.3948126801153, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI28178838, Length=335, Percent_Identity=29.2537313432836, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI28178819, Length=150, Percent_Identity=36, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI87081683, Length=360, Percent_Identity=49.7222222222222, Blast_Score=327, Evalue=8e-91,
Organism=Escherichia coli, GI1788101, Length=368, Percent_Identity=31.7934782608696, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1787381, Length=308, Percent_Identity=27.9220779220779, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=350, Percent_Identity=33.4285714285714, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=346, Percent_Identity=29.4797687861272, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=344, Percent_Identity=30.5232558139535, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=363, Percent_Identity=30.0275482093664, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=373, Percent_Identity=41.5549597855228, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6322097, Length=356, Percent_Identity=34.8314606741573, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=344, Percent_Identity=31.9767441860465, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324291, Length=342, Percent_Identity=28.6549707602339, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24643270, Length=347, Percent_Identity=33.4293948126801, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24643268, Length=347, Percent_Identity=33.4293948126801, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24661184, Length=373, Percent_Identity=31.0991957104558, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078635, Length=370, Percent_Identity=28.6486486486486, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161078637, Length=370, Percent_Identity=28.6486486486486, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161078633, Length=370, Percent_Identity=28.6486486486486, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24650122, Length=370, Percent_Identity=28.6486486486486, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI161078639, Length=370, Percent_Identity=28.6486486486486, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI281362242, Length=351, Percent_Identity=27.9202279202279, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648872, Length=351, Percent_Identity=27.9202279202279, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20130355, Length=349, Percent_Identity=25.5014326647564, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39603; Mature: 39472

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRNLFLLPGDGIGPEAMGEVRKIIAYMNEAMGAGFVTDEGLVGGSAYDAHGAAISEAD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
MVKALAADAVLFGAVGGPKWDSVPYEVRPEAGLLRLRKDLQLFANLRPAICYPALASASS
HHHHHHHHHHHEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
LKPELVEGLDILIIRELTGGVYFGEPKEIIDLGNGQKRGIDTQVYDTYEIERIAGVAFEM
CCHHHHCCCCEEEEEECCCCEEECCCHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
ARTRQNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCCEE
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDGKTGKRKALYEPVHGSAPDIAGKGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
ANPIAMIASFAMCLRYSFNLVKEADDLEKAIANVLDKGIRTGDIMADGARQVGTVEMGDA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHCCCEEHHHH
ILAEFKTLSA
HHHHHHHCCC
>Mature Secondary Structure 
TVRNLFLLPGDGIGPEAMGEVRKIIAYMNEAMGAGFVTDEGLVGGSAYDAHGAAISEAD
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
MVKALAADAVLFGAVGGPKWDSVPYEVRPEAGLLRLRKDLQLFANLRPAICYPALASASS
HHHHHHHHHHHEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
LKPELVEGLDILIIRELTGGVYFGEPKEIIDLGNGQKRGIDTQVYDTYEIERIAGVAFEM
CCHHHHCCCCEEEEEECCCCEEECCCHHHHCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
ARTRQNRVCSMEKRNVMKSGVLWNQVVTETHKAKYSDVQLEHMLADAGGMQLVRQPKQFD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCCEE
VIVTDNLFGDMLSDVAAMLTGSLGMLPSASLGAPDGKTGKRKALYEPVHGSAPDIAGKGI
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
ANPIAMIASFAMCLRYSFNLVKEADDLEKAIANVLDKGIRTGDIMADGARQVGTVEMGDA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHCCCEEHHHH
ILAEFKTLSA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA