Click here to switch to the map view.

The map label for this gene is leuD [H]

Identifier: 209551469

GI number: 209551469

Start: 4038654

End: 4039262

Strand: Direct

Name: leuD [H]

Synonym: Rleg2_3898

Alternate gene names: 209551469

Gene position: 4038654-4039262 (Clockwise)

Preceding gene: 209551468

Following gene: 209551471

Centisome position: 89.0

GC content: 58.46

Gene sequence:

>609_bases
ATGGATAAATTCGTGAAGCTCACGGGCGTTGCCGCGCCGCTGCCGGTCGTCAACGTCGACACCGACATGATCATTCCGAA
GGATTATCTGAAGACCATCAAGCGCACCGGCCTCGGCACCGGCCTTTTCGCCGAAGCCCGTTATCACGAAGATGGCTCCG
AAAACGCCGATTTCGTGCTGAACAAGCCGGCCTATCGCGATGCCAAGATCCTGGTTGCCGGTGACAATTTCGGCTGCGGC
TCCTCGCGCGAACATGCTCCGTGGGCGCTGCTCGATTTCGGCATTCGCTGCGTGATCTCCACCAGCTTCGCCGACATTTT
CTACAACAACTGTTTCAAGAACGGCATCCTGCCGATCAAGGTCAGCCAGGAAAATCTCGACAAGCTGATGGACGACGCCT
CGCGCGGCTCCAACGCCATCCTGACCGTCGACCTTGAGAATCTCGAGATCACCGGTCCCGATGGCGGCTCGATCAAGTTC
GATCTCGACGAGTTCAAGCGCCACTGCCTGCTGAACGGCCTCGACGACATCGGCCTGACGCTTGAGAAGGGCAAGGCGAT
CGACAGCTTCGAAAAGAAGAACGCCGCTTCGCACCCCTGGGCAGCTTAA

Upstream 100 bases:

>100_bases
CGCGTGGCCGCCATTTTTCAAAGATTCTCCTTCATTTCGGCGGAAAACTGGACTAGATGCAAATTCAGGAAAGTAGATTT
GCTGAAATGGAGCATGGGTC

Downstream 100 bases:

>100_bases
GCGCTCTCCAAATTCTCGATCAAGCCGGGCCTTCGCCCGGCTTTTTCTTTGATATCAGGTCAGAGAAACTTCTCTTTCCA
GAGTCTCAGCTTTTCGAGCG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MDKFVKLTGVAAPLPVVNVDTDMIIPKDYLKTIKRTGLGTGLFAEARYHEDGSENADFVLNKPAYRDAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIKVSQENLDKLMDDASRGSNAILTVDLENLEITGPDGGSIKF
DLDEFKRHCLLNGLDDIGLTLEKGKAIDSFEKKNAASHPWAA

Sequences:

>Translated_202_residues
MDKFVKLTGVAAPLPVVNVDTDMIIPKDYLKTIKRTGLGTGLFAEARYHEDGSENADFVLNKPAYRDAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIKVSQENLDKLMDDASRGSNAILTVDLENLEITGPDGGSIKF
DLDEFKRHCLLNGLDDIGLTLEKGKAIDSFEKKNAASHPWAA
>Mature_202_residues
MDKFVKLTGVAAPLPVVNVDTDMIIPKDYLKTIKRTGLGTGLFAEARYHEDGSENADFVLNKPAYRDAKILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIKVSQENLDKLMDDASRGSNAILTVDLENLEITGPDGGSIKF
DLDEFKRHCLLNGLDDIGLTLEKGKAIDSFEKKNAASHPWAA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=49.746192893401, Blast_Score=196, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=54.5454545454545, Blast_Score=209, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22097; Mature: 22097

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFVKLTGVAAPLPVVNVDTDMIIPKDYLKTIKRTGLGTGLFAEARYHEDGSENADFVL
CCCEEEECCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEE
NKPAYRDAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIK
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCEEEEEEHHHHHHHHHHHHHCCEEEEE
VSQENLDKLMDDASRGSNAILTVDLENLEITGPDGGSIKFDLDEFKRHCLLNGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LEKGKAIDSFEKKNAASHPWAA
EECCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDKFVKLTGVAAPLPVVNVDTDMIIPKDYLKTIKRTGLGTGLFAEARYHEDGSENADFVL
CCCEEEECCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEE
NKPAYRDAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIK
CCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCEEEEEEHHHHHHHHHHHHHCCEEEEE
VSQENLDKLMDDASRGSNAILTVDLENLEITGPDGGSIKFDLDEFKRHCLLNGLDDIGLT
ECHHHHHHHHHHHCCCCCEEEEEEECCEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LEKGKAIDSFEKKNAASHPWAA
EECCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA