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The map label for this gene is yjeA [H]

Identifier: 209551369

GI number: 209551369

Start: 3929467

End: 3930528

Strand: Reverse

Name: yjeA [H]

Synonym: Rleg2_3798

Alternate gene names: 209551369

Gene position: 3930528-3929467 (Counterclockwise)

Preceding gene: 209551371

Following gene: 209551368

Centisome position: 86.61

GC content: 63.47

Gene sequence:

>1062_bases
ATGAACTCTTCGGCGAAAGCGTCCCCCTGGTGGACCCCGTCCGTGCATGCAGATCGCCGCCCGTTCCTGATCGGGCGCAA
TGCGATCCAGGCGGCGTTGCGCGGCTTTTTCGCGCGCGAAGATTTCCTCGAGGTGGATACGGCGACGCTGCAAATCTCGC
CGGGCAACGAGGCGCATCTGCATGCCTTCGCCACGGAAGCGCTGACGACGGACGGGCAAAGGGCGCCGTTCTATCTGCAC
ACCTCGCCGGAATTCGCCTGCAAGAAGCTGCTTGCGGCGGGCGAGGAACGTATCTCTTGTTTTGCCCATGTCTATCGCAA
CCGCGAGCGCGGGCCGCTGCACCATCCCGAATTCACCATGCTCGAATGGTACCGGGCCGGCGAAAGCTATGAGAGCCTGA
TGATGGACTGCGTGCGGATCGTGGCGCTGGCCGCCGAAACGGCAAAGACCGAAAAGCTTGCCTATCGCGGCGGCGAGAGT
GATCCTTTTGCCGGTCCGGAGCGGATCAGCGTTGCCGAAGCCTTTGAGCGCCATGCCGGTATCGATCTTCTCTCTTCGGT
CGCCGCCGGCGGTTCGACCGATCGCGATCATCTGGCGGCCGAGATGAAGCGTGTCGGCATGCGTGTTGCCGATGACGACG
GCTGGGCCGATTTGTTCAGCCGGGTGCTGGTCGAAAAGATCGAGCCGCATCTCGGCTTCGGCCGCATCACCATCCTAGAC
GAATATCCGGTCTCGGAAGCAGCGCTCGCGCGCCCTTCGGCCCGCGATCCAAGGGTTGCCGAGCGTTTCGAGCTCTATGC
CTGCGGCGTCGAAATTGCCAACGGCTTCGGCGAACTGACCAACGCTGCCGAGCAGCGCCGGCGGTTCGAGATCGAGATGG
CGGAGAAGGCGCGTATCTATGGCGAGACCTATCCGCTCGACGAGGATTTCCTCGCCGCACTTTCGCTGATGCCCGAGGCA
AGCGGCATCGCGCTCGGCTTCGACCGGCTGGTAATGCTGGCGACGGGAGCGTCGCGCATCGATCAGGTGCTCTGGGCGCC
GGTAGCGGAGTATGGACGATGA

Upstream 100 bases:

>100_bases
ACGCCTCTGAGACGCAAATATTTTTCTTTGGGGCGCAACTAACCTAAAATCCGGGAAATAGCCATCCCCATACCGGCTTC
TCCGGCGAAGAAAGCTTGGA

Downstream 100 bases:

>100_bases
ATGTCGTCAAACCGCTCAAGAGTGCCGACGATCTGGTGATGGCGGGGCTGGTCGCGCCGGCCGATCGCAGAGCGCTCGAG
GAGGTTGCCGCGCGTTATGC

Product: lysine--tRNA ligase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNSSAKASPWWTPSVHADRRPFLIGRNAIQAALRGFFAREDFLEVDTATLQISPGNEAHLHAFATEALTTDGQRAPFYLH
TSPEFACKKLLAAGEERISCFAHVYRNRERGPLHHPEFTMLEWYRAGESYESLMMDCVRIVALAAETAKTEKLAYRGGES
DPFAGPERISVAEAFERHAGIDLLSSVAAGGSTDRDHLAAEMKRVGMRVADDDGWADLFSRVLVEKIEPHLGFGRITILD
EYPVSEAALARPSARDPRVAERFELYACGVEIANGFGELTNAAEQRRRFEIEMAEKARIYGETYPLDEDFLAALSLMPEA
SGIALGFDRLVMLATGASRIDQVLWAPVAEYGR

Sequences:

>Translated_353_residues
MNSSAKASPWWTPSVHADRRPFLIGRNAIQAALRGFFAREDFLEVDTATLQISPGNEAHLHAFATEALTTDGQRAPFYLH
TSPEFACKKLLAAGEERISCFAHVYRNRERGPLHHPEFTMLEWYRAGESYESLMMDCVRIVALAAETAKTEKLAYRGGES
DPFAGPERISVAEAFERHAGIDLLSSVAAGGSTDRDHLAAEMKRVGMRVADDDGWADLFSRVLVEKIEPHLGFGRITILD
EYPVSEAALARPSARDPRVAERFELYACGVEIANGFGELTNAAEQRRRFEIEMAEKARIYGETYPLDEDFLAALSLMPEA
SGIALGFDRLVMLATGASRIDQVLWAPVAEYGR
>Mature_353_residues
MNSSAKASPWWTPSVHADRRPFLIGRNAIQAALRGFFAREDFLEVDTATLQISPGNEAHLHAFATEALTTDGQRAPFYLH
TSPEFACKKLLAAGEERISCFAHVYRNRERGPLHHPEFTMLEWYRAGESYESLMMDCVRIVALAAETAKTEKLAYRGGES
DPFAGPERISVAEAFERHAGIDLLSSVAAGGSTDRDHLAAEMKRVGMRVADDDGWADLFSRVLVEKIEPHLGFGRITILD
EYPVSEAALARPSARDPRVAERFELYACGVEIANGFGELTNAAEQRRRFEIEMAEKARIYGETYPLDEDFLAALSLMPEA
SGIALGFDRLVMLATGASRIDQVLWAPVAEYGR

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=339, Percent_Identity=27.4336283185841, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI5031815, Length=339, Percent_Identity=27.4336283185841, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87082379, Length=337, Percent_Identity=36.4985163204748, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1790571, Length=334, Percent_Identity=31.7365269461078, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789256, Length=333, Percent_Identity=30.6306306306306, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71994340, Length=339, Percent_Identity=28.9085545722714, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535927, Length=339, Percent_Identity=28.9085545722714, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535925, Length=339, Percent_Identity=28.9085545722714, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320242, Length=348, Percent_Identity=26.1494252873563, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24640851, Length=338, Percent_Identity=26.6272189349112, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24640849, Length=338, Percent_Identity=26.6272189349112, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 39129; Mature: 39129

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSAKASPWWTPSVHADRRPFLIGRNAIQAALRGFFAREDFLEVDTATLQISPGNEAHL
CCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCHH
HAFATEALTTDGQRAPFYLHTSPEFACKKLLAAGEERISCFAHVYRNRERGPLHHPEFTM
HHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHH
LEWYRAGESYESLMMDCVRIVALAAETAKTEKLAYRGGESDPFAGPERISVAEAFERHAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
IDLLSSVAAGGSTDRDHLAAEMKRVGMRVADDDGWADLFSRVLVEKIEPHLGFGRITILD
HHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
EYPVSEAALARPSARDPRVAERFELYACGVEIANGFGELTNAAEQRRRFEIEMAEKARIY
CCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GETYPLDEDFLAALSLMPEASGIALGFDRLVMLATGASRIDQVLWAPVAEYGR
CCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSSAKASPWWTPSVHADRRPFLIGRNAIQAALRGFFAREDFLEVDTATLQISPGNEAHL
CCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCHH
HAFATEALTTDGQRAPFYLHTSPEFACKKLLAAGEERISCFAHVYRNRERGPLHHPEFTM
HHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHH
LEWYRAGESYESLMMDCVRIVALAAETAKTEKLAYRGGESDPFAGPERISVAEAFERHAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
IDLLSSVAAGGSTDRDHLAAEMKRVGMRVADDDGWADLFSRVLVEKIEPHLGFGRITILD
HHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
EYPVSEAALARPSARDPRVAERFELYACGVEIANGFGELTNAAEQRRRFEIEMAEKARIY
CCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GETYPLDEDFLAALSLMPEASGIALGFDRLVMLATGASRIDQVLWAPVAEYGR
CCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA