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The map label for this gene is trmD [H]

Identifier: 209551325

GI number: 209551325

Start: 3879029

End: 3879730

Strand: Direct

Name: trmD [H]

Synonym: Rleg2_3754

Alternate gene names: 209551325

Gene position: 3879029-3879730 (Clockwise)

Preceding gene: 209551324

Following gene: 209551326

Centisome position: 85.48

GC content: 65.67

Gene sequence:

>702_bases
ATGGCTTTCCGGGCGAGCGTGCTGACACTCTATCCGGAAATGTTTCCGGGGCATCTGGGCTTCTCGCTCGCCGGCAAGGC
GATGGAGCGCGGGCAATGGTCGCTGGATGCGGTGCAGATCCGCGATTTCGCCACCGACAAGCACCGCACCGTCGACGACA
CCCCGGCCGGCGGCGGCGCCGGCATGGTGCTGAAACCCGACGTTCTCGCCCGTGCGATCGACAGCACCGCGGAAAACGAT
ACCCGCCCGCGCCTGCTGATGAGCCCGCGCGGCCGGCCGCTGACACAGGAGCGTGTACGCGAGCTTGCTTCAGGCGACGG
CGTCATCATCGTCTGCGGCCGCTTCGAGGGCGTCGATCAGCGGGTGATCGAGGCGCGCGGCCTGGAAGAGGTTTCGATCG
GCGACTACGTGCTGTCAGGCGGCGAGCCGGCAGCGCTGATCGTGCTCGACGCGATCATCCGCATCCTGCCCGGTGTCATG
GGCAACGATCTTTCCGGCCTGCATGAAAGCTTCGAAGGCGGGCTGCTGGAACATCCGCATTATACCAGGCCGCAGGCGTG
GGAAGGCTGGGAAATTCCCTCGATCCTGACCTCCGGCAATCACGGCGCCATCGAAAAGTGGCGGCACCAGGAGGCGGTGC
GGCTGACGCGGGAACGGCGGCCGGATCTTCTGGAAAAGGCGAAAACCGAGAAAAACGGCTGA

Upstream 100 bases:

>100_bases
AGGCCGGAACGCTGCTGATCGACCCGCTGGCGGCAGGGCTGGTCGACGACCCCGAAGAGCTCTCGAAATTCACCGCGGAC
AAGCCGAAAAAGAAGAAGTG

Downstream 100 bases:

>100_bases
TTCCAGCCTCCTCTTCACAAAAATGTCGTGCGAGGGATGACAAGCCCCGGCCTTTCGTGTATTGGCGCACGCGGAAATGG
GGTTATCCCCGTCTGCCAGC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MAFRASVLTLYPEMFPGHLGFSLAGKAMERGQWSLDAVQIRDFATDKHRTVDDTPAGGGAGMVLKPDVLARAIDSTAEND
TRPRLLMSPRGRPLTQERVRELASGDGVIIVCGRFEGVDQRVIEARGLEEVSIGDYVLSGGEPAALIVLDAIIRILPGVM
GNDLSGLHESFEGGLLEHPHYTRPQAWEGWEIPSILTSGNHGAIEKWRHQEAVRLTRERRPDLLEKAKTEKNG

Sequences:

>Translated_233_residues
MAFRASVLTLYPEMFPGHLGFSLAGKAMERGQWSLDAVQIRDFATDKHRTVDDTPAGGGAGMVLKPDVLARAIDSTAEND
TRPRLLMSPRGRPLTQERVRELASGDGVIIVCGRFEGVDQRVIEARGLEEVSIGDYVLSGGEPAALIVLDAIIRILPGVM
GNDLSGLHESFEGGLLEHPHYTRPQAWEGWEIPSILTSGNHGAIEKWRHQEAVRLTRERRPDLLEKAKTEKNG
>Mature_232_residues
AFRASVLTLYPEMFPGHLGFSLAGKAMERGQWSLDAVQIRDFATDKHRTVDDTPAGGGAGMVLKPDVLARAIDSTAENDT
RPRLLMSPRGRPLTQERVRELASGDGVIIVCGRFEGVDQRVIEARGLEEVSIGDYVLSGGEPAALIVLDAIIRILPGVMG
NDLSGLHESFEGGLLEHPHYTRPQAWEGWEIPSILTSGNHGAIEKWRHQEAVRLTRERRPDLLEKAKTEKNG

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=230, Percent_Identity=44.7826086956522, Blast_Score=194, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25580; Mature: 25449

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFRASVLTLYPEMFPGHLGFSLAGKAMERGQWSLDAVQIRDFATDKHRTVDDTPAGGGA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
GMVLKPDVLARAIDSTAENDTRPRLLMSPRGRPLTQERVRELASGDGVIIVCGRFEGVDQ
CEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHH
RVIEARGLEEVSIGDYVLSGGEPAALIVLDAIIRILPGVMGNDLSGLHESFEGGLLEHPH
HHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
YTRPQAWEGWEIPSILTSGNHGAIEKWRHQEAVRLTRERRPDLLEKAKTEKNG
CCCCCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AFRASVLTLYPEMFPGHLGFSLAGKAMERGQWSLDAVQIRDFATDKHRTVDDTPAGGGA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
GMVLKPDVLARAIDSTAENDTRPRLLMSPRGRPLTQERVRELASGDGVIIVCGRFEGVDQ
CEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHH
RVIEARGLEEVSIGDYVLSGGEPAALIVLDAIIRILPGVMGNDLSGLHESFEGGLLEHPH
HHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
YTRPQAWEGWEIPSILTSGNHGAIEKWRHQEAVRLTRERRPDLLEKAKTEKNG
CCCCCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA