Click here to switch to the map view.

The map label for this gene is ligT [C]

Identifier: 209551301

GI number: 209551301

Start: 3856092

End: 3856685

Strand: Reverse

Name: ligT [C]

Synonym: Rleg2_3730

Alternate gene names: 209551301

Gene position: 3856685-3856092 (Counterclockwise)

Preceding gene: 209551302

Following gene: 209551300

Centisome position: 84.99

GC content: 63.97

Gene sequence:

>594_bases
ATGCCGAGACTGTTTACCGCCCTCGAAATTCCGCGCAATGCGGCGATGAGCCTTTCATTGCTGCGCGGTGGCCTCCCCGG
AGCACGATGGATCGATGTGGAGAATTACCACATCACCCTGCGCTTCATCGGTGATGTCGACGGCCGTACGGCCGATGAAA
TCGTAGAACGCCTTGACCGGATCGATCGGCCGGAATTCCAGTTCCGGCTCGAAGGCATCGGTTCCTTCGGCTCGAAGAAG
CCGCATTCGGTCTGGGCCGGCGTTTCGCAATCGCCGGAGATGTACGCGCTGCAGGGCGAGATCGAGCGGATCTGCCAGCG
CATCGGCCTGCCGCCGGATCCGCGCAAGTTCATGCCGCATGTAACGCTGGCGCGGCTCAAATCGTCGCGGCTCGACGATG
TCGTGCAATATCTGGCCGGACGCGGCAACTTCCACACGTCGACCTTCACGGCGCCGCGCTTCGTGCTGCTGTCGTCGCGC
GAATCGGTCGGCGGCGGGCCCTACCTCACCGAGGAAGTGTTCCCGCTGCACGAGGCGCGCTCGGCGCCCAGCGCTTCGAG
CAGGCTGCTACAGCCGGTCAAGAGCCTGGTGTAG

Upstream 100 bases:

>100_bases
TCGTCGGAACGATTTCTACTGTGAAGAAATAGGGGCTTGCGCGGTTATTGATTGCATGATTCCGTGTAAGCACCTGCAAA
AGATTCGGGGAGTGCTCGTT

Downstream 100 bases:

>100_bases
ACCAGCTCGAAACCTGCCGGGCCTCCATAATAGGGATCGGGAATATCCTCTCCCGTTCCAAGCGCGATATCGCCGAACAG
GCGGATGCTCGCCTCAGGCG

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MPRLFTALEIPRNAAMSLSLLRGGLPGARWIDVENYHITLRFIGDVDGRTADEIVERLDRIDRPEFQFRLEGIGSFGSKK
PHSVWAGVSQSPEMYALQGEIERICQRIGLPPDPRKFMPHVTLARLKSSRLDDVVQYLAGRGNFHTSTFTAPRFVLLSSR
ESVGGGPYLTEEVFPLHEARSAPSASSRLLQPVKSLV

Sequences:

>Translated_197_residues
MPRLFTALEIPRNAAMSLSLLRGGLPGARWIDVENYHITLRFIGDVDGRTADEIVERLDRIDRPEFQFRLEGIGSFGSKK
PHSVWAGVSQSPEMYALQGEIERICQRIGLPPDPRKFMPHVTLARLKSSRLDDVVQYLAGRGNFHTSTFTAPRFVLLSSR
ESVGGGPYLTEEVFPLHEARSAPSASSRLLQPVKSLV
>Mature_196_residues
PRLFTALEIPRNAAMSLSLLRGGLPGARWIDVENYHITLRFIGDVDGRTADEIVERLDRIDRPEFQFRLEGIGSFGSKKP
HSVWAGVSQSPEMYALQGEIERICQRIGLPPDPRKFMPHVTLARLKSSRLDDVVQYLAGRGNFHTSTFTAPRFVLLSSRE
SVGGGPYLTEEVFPLHEARSAPSASSRLLQPVKSLV

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21999; Mature: 21867

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFTALEIPRNAAMSLSLLRGGLPGARWIDVENYHITLRFIGDVDGRTADEIVERLDR
CCCHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHH
IDRPEFQFRLEGIGSFGSKKPHSVWAGVSQSPEMYALQGEIERICQRIGLPPDPRKFMPH
CCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHCCH
VTLARLKSSRLDDVVQYLAGRGNFHTSTFTAPRFVLLSSRESVGGGPYLTEEVFPLHEAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHC
SAPSASSRLLQPVKSLV
CCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
PRLFTALEIPRNAAMSLSLLRGGLPGARWIDVENYHITLRFIGDVDGRTADEIVERLDR
CCHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHH
IDRPEFQFRLEGIGSFGSKKPHSVWAGVSQSPEMYALQGEIERICQRIGLPPDPRKFMPH
CCCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHCCH
VTLARLKSSRLDDVVQYLAGRGNFHTSTFTAPRFVLLSSRESVGGGPYLTEEVFPLHEAR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHC
SAPSASSRLLQPVKSLV
CCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]