Click here to switch to the map view.

The map label for this gene is purA [H]

Identifier: 209551289

GI number: 209551289

Start: 3844697

End: 3845905

Strand: Direct

Name: purA [H]

Synonym: Rleg2_3717

Alternate gene names: 209551289

Gene position: 3844697-3845905 (Clockwise)

Preceding gene: 209551288

Following gene: 209551291

Centisome position: 84.72

GC content: 67.33

Gene sequence:

>1209_bases
ATGAGCACTCGAGCACAGGCCGTGATCGGCGCGCTTTATGGCGACGAAGGCAAGGGGCTGATGGTCGATCGCCTTGCCGC
AGCCGCGCCGGGAGCCCTGGTAGTTCGCAGCAATGGCGGCGCTCAGGCCGGCCACACCGTGGTCGGTTCCGCCGGCCTGC
GCCATGTCTTCCATCATATCGGCTCGGGCAGCTTTGCCGGGGCCGCCACGCATTTCAGCCGCTTTTTCGTCGCCCATCCG
ATGCTGCTGCTCGACGAGCTGGCGGCCCTCAGCCAGCTCGGCGTGAAGCCCGAGATCAGCAGCGACCCGCGTGCCCCGGT
CACCACGCCGTTCGATGTTATCATCAACCAGGCCTTGGAGCTGGCGCGTGGCGCTGCCCGTCATGGCAGTTGCGGCCTCG
GCTTCGGCGAGACCATCGAACGCAATCTGCGCCCGGAATTCGCCCTCTCGACCAAGGACCTGTTCCGCCCCGATCTGCAC
GCCCGCCTTGTTTTTATCCGCGATGCCTGGGTGCCGATGCGGCTGGCCGCGCTCGGCATCACCGCCCTGCCCGCAGAACT
GGCCACCGCGCTGGCTGACGACACCACCATCGCCCGCTTCGAGGCCGATTGCGCCGCCTATCTCGACCGCGTCACGCTCT
GGCCCGACCGGCGGCTGTGCGAACGCGGCACCGTCATCTTCGAAGCGGCCCAGGGCCTCGGGCTTGATCAGGACCGTGGC
GTCTTTCCCCACGTCACCCGCTCGAATACCGGGCTTGCCAACATGCTCGCTATCGCCGCCGAGGCCGGCATCACCGAGCT
GGAGGCGACATATGCGACTCGCTGCTACACCACCCGGCACGGCGCAGGGCCCCTCAAGGGGGAAGTTCCGACGCTTCCCG
GCGTCAATGTCGTCGACCCCACCAACGCGCCGAACGAGTGGCAGGGGTGCCTGAGGCTTGCGCCGCTCGTTCTCGGCCTG
TTGCGTGAGGCCATCACGGGCGATCTGAGCTTGGACCGCGGCGGTATCGCCGTCAGAGCCGGACTGGCGGTGACCTGCCT
CGATCAGGCGGAGGATGGTTTTGCGGTGACGGATTGCGGAGAGGTGATGCCGCTTGATCCTGCCAAAGCGGCGTCCGAGA
TAGCCGATCTGGTCGGCCTGCCGCTATGGGCCGAAAGCTGGGGACCGCGTCGCGGTGACGTCAGACTACGTGTCGATGCC
GCCGCGTAA

Upstream 100 bases:

>100_bases
AGCGGTTCTGAGTTCCCATAAAAAGTTGAACCGCTCCGGCCTCATATCGTAGAGAACAAAAGCCCCGGCGCGCTGCGGGA
ACCATCTTGAGGAGCAGAAG

Downstream 100 bases:

>100_bases
AAAAGAGCCCGCGCGGCAATGCCGGCGGGCTCATATCCGGTACGAAAGCGACCGTGACTACTTCTTGAGATTGCGCTTGC
CGAGTGTGCGCAGGCGCAGC

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSTRAQAVIGALYGDEGKGLMVDRLAAAAPGALVVRSNGGAQAGHTVVGSAGLRHVFHHIGSGSFAGAATHFSRFFVAHP
MLLLDELAALSQLGVKPEISSDPRAPVTTPFDVIINQALELARGAARHGSCGLGFGETIERNLRPEFALSTKDLFRPDLH
ARLVFIRDAWVPMRLAALGITALPAELATALADDTTIARFEADCAAYLDRVTLWPDRRLCERGTVIFEAAQGLGLDQDRG
VFPHVTRSNTGLANMLAIAAEAGITELEATYATRCYTTRHGAGPLKGEVPTLPGVNVVDPTNAPNEWQGCLRLAPLVLGL
LREAITGDLSLDRGGIAVRAGLAVTCLDQAEDGFAVTDCGEVMPLDPAKAASEIADLVGLPLWAESWGPRRGDVRLRVDA
AA

Sequences:

>Translated_402_residues
MSTRAQAVIGALYGDEGKGLMVDRLAAAAPGALVVRSNGGAQAGHTVVGSAGLRHVFHHIGSGSFAGAATHFSRFFVAHP
MLLLDELAALSQLGVKPEISSDPRAPVTTPFDVIINQALELARGAARHGSCGLGFGETIERNLRPEFALSTKDLFRPDLH
ARLVFIRDAWVPMRLAALGITALPAELATALADDTTIARFEADCAAYLDRVTLWPDRRLCERGTVIFEAAQGLGLDQDRG
VFPHVTRSNTGLANMLAIAAEAGITELEATYATRCYTTRHGAGPLKGEVPTLPGVNVVDPTNAPNEWQGCLRLAPLVLGL
LREAITGDLSLDRGGIAVRAGLAVTCLDQAEDGFAVTDCGEVMPLDPAKAASEIADLVGLPLWAESWGPRRGDVRLRVDA
AA
>Mature_401_residues
STRAQAVIGALYGDEGKGLMVDRLAAAAPGALVVRSNGGAQAGHTVVGSAGLRHVFHHIGSGSFAGAATHFSRFFVAHPM
LLLDELAALSQLGVKPEISSDPRAPVTTPFDVIINQALELARGAARHGSCGLGFGETIERNLRPEFALSTKDLFRPDLHA
RLVFIRDAWVPMRLAALGITALPAELATALADDTTIARFEADCAAYLDRVTLWPDRRLCERGTVIFEAAQGLGLDQDRGV
FPHVTRSNTGLANMLAIAAEAGITELEATYATRCYTTRHGAGPLKGEVPTLPGVNVVDPTNAPNEWQGCLRLAPLVLGLL
REAITGDLSLDRGGIAVRAGLAVTCLDQAEDGFAVTDCGEVMPLDPAKAASEIADLVGLPLWAESWGPRRGDVRLRVDAA
A

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790620, Length=294, Percent_Identity=30.6122448979592, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 42386; Mature: 42255

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRAQAVIGALYGDEGKGLMVDRLAAAAPGALVVRSNGGAQAGHTVVGSAGLRHVFHHI
CCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEEECHHHHHHHHHHC
GSGSFAGAATHFSRFFVAHPMLLLDELAALSQLGVKPEISSDPRAPVTTPFDVIINQALE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LARGAARHGSCGLGFGETIERNLRPEFALSTKDLFRPDLHARLVFIRDAWVPMRLAALGI
HHHCCCCCCCCCCCCHHHHHHCCCCCEEECHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
TALPAELATALADDTTIARFEADCAAYLDRVTLWPDRRLCERGTVIFEAAQGLGLDQDRG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEHHHCCCCCCCCC
VFPHVTRSNTGLANMLAIAAEAGITELEATYATRCYTTRHGAGPLKGEVPTLPGVNVVDP
CCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCEECC
TNAPNEWQGCLRLAPLVLGLLREAITGDLSLDRGGIAVRAGLAVTCLDQAEDGFAVTDCG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCEEEEEECCCCCCEEEECCC
EVMPLDPAKAASEIADLVGLPLWAESWGPRRGDVRLRVDAAA
CCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
STRAQAVIGALYGDEGKGLMVDRLAAAAPGALVVRSNGGAQAGHTVVGSAGLRHVFHHI
CCHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEEECHHHHHHHHHHC
GSGSFAGAATHFSRFFVAHPMLLLDELAALSQLGVKPEISSDPRAPVTTPFDVIINQALE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LARGAARHGSCGLGFGETIERNLRPEFALSTKDLFRPDLHARLVFIRDAWVPMRLAALGI
HHHCCCCCCCCCCCCHHHHHHCCCCCEEECHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
TALPAELATALADDTTIARFEADCAAYLDRVTLWPDRRLCERGTVIFEAAQGLGLDQDRG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEEHHHCCCCCCCCC
VFPHVTRSNTGLANMLAIAAEAGITELEATYATRCYTTRHGAGPLKGEVPTLPGVNVVDP
CCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCEECC
TNAPNEWQGCLRLAPLVLGLLREAITGDLSLDRGGIAVRAGLAVTCLDQAEDGFAVTDCG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEECCEEEEEECCCCCCEEEECCC
EVMPLDPAKAASEIADLVGLPLWAESWGPRRGDVRLRVDAAA
CCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA