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The map label for this gene is gltX [H]

Identifier: 209551131

GI number: 209551131

Start: 3684113

End: 3684994

Strand: Direct

Name: gltX [H]

Synonym: Rleg2_3555

Alternate gene names: 209551131

Gene position: 3684113-3684994 (Clockwise)

Preceding gene: 209551128

Following gene: 209551133

Centisome position: 81.18

GC content: 65.65

Gene sequence:

>882_bases
ATGACCACGAGCGAGCGTCAAAAACCTGTCTTTCGTTTTGCGCCGAGCCCCAACGGGCAGCTGCATCTCGGTCATGCCTT
CTCGGCCCTCTTGAACCGCGACATGGCGGAGGCCGAGCATGGCCGCCTGCTGTTGCGCATCGAGGATATCGACCAGACGC
GCTGCACGCCCGAGTTCGAGGCCGGCATCCTCAGGGATCTCGACTGGCTGGGGATTGGCTGGGAAAGCCCGGTGCGGCGA
CAGTCGGAACATTTCCCCGAATATCAGGCGGCACTGCACGCGCTGATCGAACGCGGCCTGGTCTATCCGGCCTTCCTGAC
GCGCGGCGAGGTGAAGACGCGCGTTACAGCCTATGAGGCGGCCGGCAGGTCCTGGCCGCGCGATCCCGACGGCACGCCGC
ATTACCCCGCAAGCGATCGCGAGCGCCCGGAAGACGAATGGCGGGAGATGCTGACCTCGGGGAAGAAACATGCCTGGCGC
CTCGATATGCGCAAGGCGCTCGACCTGATCGGCGAGATGCTGTTCTGGACCGAAACCGGCGACGGCAGATCGGGCGAGAT
CGCCGCCGAGCCGGACGTCTGGGGCGATGTCATCCTGTCGCGCTCGGACGCCCCGTCGAGCTATCATCTTTCGGTGGTGG
TCGACGATGCGCTCCAGGGTGTCACCCATGTGGTGCGCGGGCTCGACCTCTTCCCCGCCACCTCGGTGCACCGGCTGCTG
CAAGTCCTGCTCGGCCTGCCGCAGCCCCTCTATCACCACCATCGTCTCATTCTCGACGATGCCGGCCGCAAGCTTTCGAA
AAGCCAGGGCGACAGCGGGATTGGCGAATTGCGGGCCCGCGGCATGTCAGGGGCCGATATTCGCCGCCTTGTCGGGCTCT
GA

Upstream 100 bases:

>100_bases
AAGCAGCGCTTCGAGCCCTTGCCTGACGTCGTCTTCGTTGCGGATGATCTGCACATCGCCTCGCAGTGGTTCGAAACCTA
AATCCATGGCAGAAGAAAGC

Downstream 100 bases:

>100_bases
TGCCCGTTCGGCACGCTCTCTGCCGACGATCGAAAGCGTGTGGGAAAACATCCGGAAGACGCGGAACTTGATCAGCGCCG
CATTGATCTCGGCGCCGATG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTTSERQKPVFRFAPSPNGQLHLGHAFSALLNRDMAEAEHGRLLLRIEDIDQTRCTPEFEAGILRDLDWLGIGWESPVRR
QSEHFPEYQAALHALIERGLVYPAFLTRGEVKTRVTAYEAAGRSWPRDPDGTPHYPASDRERPEDEWREMLTSGKKHAWR
LDMRKALDLIGEMLFWTETGDGRSGEIAAEPDVWGDVILSRSDAPSSYHLSVVVDDALQGVTHVVRGLDLFPATSVHRLL
QVLLGLPQPLYHHHRLILDDAGRKLSKSQGDSGIGELRARGMSGADIRRLVGL

Sequences:

>Translated_293_residues
MTTSERQKPVFRFAPSPNGQLHLGHAFSALLNRDMAEAEHGRLLLRIEDIDQTRCTPEFEAGILRDLDWLGIGWESPVRR
QSEHFPEYQAALHALIERGLVYPAFLTRGEVKTRVTAYEAAGRSWPRDPDGTPHYPASDRERPEDEWREMLTSGKKHAWR
LDMRKALDLIGEMLFWTETGDGRSGEIAAEPDVWGDVILSRSDAPSSYHLSVVVDDALQGVTHVVRGLDLFPATSVHRLL
QVLLGLPQPLYHHHRLILDDAGRKLSKSQGDSGIGELRARGMSGADIRRLVGL
>Mature_292_residues
TTSERQKPVFRFAPSPNGQLHLGHAFSALLNRDMAEAEHGRLLLRIEDIDQTRCTPEFEAGILRDLDWLGIGWESPVRRQ
SEHFPEYQAALHALIERGLVYPAFLTRGEVKTRVTAYEAAGRSWPRDPDGTPHYPASDRERPEDEWREMLTSGKKHAWRL
DMRKALDLIGEMLFWTETGDGRSGEIAAEPDVWGDVILSRSDAPSSYHLSVVVDDALQGVTHVVRGLDLFPATSVHRLLQ
VLLGLPQPLYHHHRLILDDAGRKLSKSQGDSGIGELRARGMSGADIRRLVGL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=280, Percent_Identity=33.5714285714286, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1788743, Length=270, Percent_Identity=30.7407407407407, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI226510925, Length=259, Percent_Identity=34.3629343629344, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786895, Length=115, Percent_Identity=34.7826086956522, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=283, Percent_Identity=26.5017667844523, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324540, Length=278, Percent_Identity=31.6546762589928, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321192, Length=93, Percent_Identity=39.7849462365591, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324742, Length=102, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665321, Length=279, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=6e-28,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020752
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 32917; Mature: 32785

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSERQKPVFRFAPSPNGQLHLGHAFSALLNRDMAEAEHGRLLLRIEDIDQTRCTPEFE
CCCCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHH
AGILRDLDWLGIGWESPVRRQSEHFPEYQAALHALIERGLVYPAFLTRGEVKTRVTAYEA
HHHHHCCHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHH
AGRSWPRDPDGTPHYPASDRERPEDEWREMLTSGKKHAWRLDMRKALDLIGEMLFWTETG
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECC
DGRSGEIAAEPDVWGDVILSRSDAPSSYHLSVVVDDALQGVTHVVRGLDLFPATSVHRLL
CCCCCCEEECCCCHHHHHEECCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHH
QVLLGLPQPLYHHHRLILDDAGRKLSKSQGDSGIGELRARGMSGADIRRLVGL
HHHHCCCCHHHHHCEEEEHHHCCHHHHCCCCCCHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure 
TTSERQKPVFRFAPSPNGQLHLGHAFSALLNRDMAEAEHGRLLLRIEDIDQTRCTPEFE
CCCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHH
AGILRDLDWLGIGWESPVRRQSEHFPEYQAALHALIERGLVYPAFLTRGEVKTRVTAYEA
HHHHHCCHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHH
AGRSWPRDPDGTPHYPASDRERPEDEWREMLTSGKKHAWRLDMRKALDLIGEMLFWTETG
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECC
DGRSGEIAAEPDVWGDVILSRSDAPSSYHLSVVVDDALQGVTHVVRGLDLFPATSVHRLL
CCCCCCEEECCCCHHHHHEECCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHH
QVLLGLPQPLYHHHRLILDDAGRKLSKSQGDSGIGELRARGMSGADIRRLVGL
HHHHCCCCHHHHHCEEEEHHHCCHHHHCCCCCCHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]