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The map label for this gene is acsA [H]

Identifier: 209551119

GI number: 209551119

Start: 3673001

End: 3674632

Strand: Reverse

Name: acsA [H]

Synonym: Rleg2_3543

Alternate gene names: 209551119

Gene position: 3674632-3673001 (Counterclockwise)

Preceding gene: 209551121

Following gene: 209551115

Centisome position: 80.98

GC content: 64.09

Gene sequence:

>1632_bases
ATGATACTGCCGTCCCACAATCGCTACGACGATCTCTATCGCGATTTCTCGTGGCGGATTCCCGAGGATTTCAATATCGG
CCGCGCCGTCAGCGACGACTGGGCGGCGAGGGCGCCGAAGCGCGTCTGCCTCGAACATTTCAGCCCGGACGGGCATCACC
GTACTTTGACCTATCGTGCGCTCGCCGACCGTTCCTCGGCCTTTGCAAATGCGCTGGTTTCGCTTGGCATTAAACGTGGC
GATCGCGTCGCGCTGCTCCTGCCGCAATCCTTCGAAACGGTGATCGCGCATGTGGCGATCTACAAGACCGGCGTGATCGC
GCTGCCGCTGGCGCTGCTTTTCGGCGTCGAGGCGCTGGAGTACCGGCTGAAGGCGGCAGGCGTGGCGGCCGTCGTCACCA
ATGGCTTCGGTCTCGAGCGCATCCGGCAGATCCGCGAGCACCTGCCGATGTTGAAACATATTGTCAGCATCGATGGGGCG
AGTGCCGATGCGGCCGCCTTCGGCGAACTGGTGGATGGCCATTCCCCCGTCTTCGATGTCGTCAAGACCGGTCCGGACGA
TCCGGCGTTGATGATTTTCACCTCGGGAACGACGGGGCCGCCGAAGGGCGCACTGCATGGCCACCGTGTCCTGCCCGGCC
ATATTCCCGGCATGCAGTTTGCCCATGAGGGTTTTCCGAAAGCGGGCGACAAGGTCTGGACGCCGTCCGACTGGGCCTGG
GCCGGGGGGCTGCTCAACGCGCTGCTGCCGAGCCTGCTGCTCGGCGTTCCCGTCGTCTCGTCGCCGGCGCAGAAATTCGA
CGCCGATATGGCCTACCGCATCATGGCCGAGATGGGGGTGCGCAACGCCTTCATTCCGCCGACGGCATTGAGGCTGATGC
GCTCTGTTGCCGATCCGCGTTCGAAATACGATCTGGTGCTGCGCACCATCGGCTCGGCCGGAGAGGCGCTCGGCCGTGAG
ACCTATGAATGGGCACAGCGCACGCTTGGCATCACCGTCAACGAATTCTACGGCCAGACGGAGTGCAATTTCGTGCTCTC
GTCGAGCGCTGCCTACGGGGTGACCAGGGCCGGCGCCATCGGCCGGGCAGTGCCCGGGCATCGCGTCGCAATCGTCAGCG
AAGCCGGCGACGAATTGCCGGCGGGCGAACCGGGTCAGATCGCCATCGCCAGTCCCGATCCGGTCATGTTCCTCGGCTAT
TGGGACGATGCGGCGGCGACGGCGCGAAAATTCGCCAAAGGCTGGCTGCTCACCGGCGATATCGGCCGGCAGGATGCTGA
TGGCTACGTTACCTTCGAAGGCCGCGACGACGATGTCATCACCTCGTCCGGATATCGCATCGGCCCGGCCGAAATCGAGG
ATTGCCTGATCGGCCACCCCGCCGTGCAGCTTGCCGCCGCGGTCGGCAAACCCGATGCGGTGCGCACCGAGATCGTCAAG
GCCTATATCGTTCTGTCGCCGGGCCATCAGCCAAGCGAGGCGCTGGCTGCCGATATCAGGGAATGGGTAAAGACGCGGCT
TTCAATGCATGAATATCCGCGCGAGGTGGAATTCATCGACGCCTTGCCGCTGACGACCACCGGCAAGGTGATCCGCCGGC
TGCTGCGCGAGAAGGCGGCGGCCGAAGATTAA

Upstream 100 bases:

>100_bases
TGGGCTTTGAAGCTGGGCCCGGTCATCGCCGTCCGGTCCGGTCGCTTGCGCAATTGCAGCACAAGGTGTTAGCTGGTGCA
GCATTCACTGGATTTCGCCT

Downstream 100 bases:

>100_bases
AGACCAGGGGTTCAGCCCCGGCCGGCGAGCGACATGATCTTCTCGCGCAGCATCTTCGCCATGTTGCGGGTGCTGCGGTA
CATGTGGAGCTGGGCTGAGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acetyl-CoA synthase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MILPSHNRYDDLYRDFSWRIPEDFNIGRAVSDDWAARAPKRVCLEHFSPDGHHRTLTYRALADRSSAFANALVSLGIKRG
DRVALLLPQSFETVIAHVAIYKTGVIALPLALLFGVEALEYRLKAAGVAAVVTNGFGLERIRQIREHLPMLKHIVSIDGA
SADAAAFGELVDGHSPVFDVVKTGPDDPALMIFTSGTTGPPKGALHGHRVLPGHIPGMQFAHEGFPKAGDKVWTPSDWAW
AGGLLNALLPSLLLGVPVVSSPAQKFDADMAYRIMAEMGVRNAFIPPTALRLMRSVADPRSKYDLVLRTIGSAGEALGRE
TYEWAQRTLGITVNEFYGQTECNFVLSSSAAYGVTRAGAIGRAVPGHRVAIVSEAGDELPAGEPGQIAIASPDPVMFLGY
WDDAAATARKFAKGWLLTGDIGRQDADGYVTFEGRDDDVITSSGYRIGPAEIEDCLIGHPAVQLAAAVGKPDAVRTEIVK
AYIVLSPGHQPSEALAADIREWVKTRLSMHEYPREVEFIDALPLTTTGKVIRRLLREKAAAED

Sequences:

>Translated_543_residues
MILPSHNRYDDLYRDFSWRIPEDFNIGRAVSDDWAARAPKRVCLEHFSPDGHHRTLTYRALADRSSAFANALVSLGIKRG
DRVALLLPQSFETVIAHVAIYKTGVIALPLALLFGVEALEYRLKAAGVAAVVTNGFGLERIRQIREHLPMLKHIVSIDGA
SADAAAFGELVDGHSPVFDVVKTGPDDPALMIFTSGTTGPPKGALHGHRVLPGHIPGMQFAHEGFPKAGDKVWTPSDWAW
AGGLLNALLPSLLLGVPVVSSPAQKFDADMAYRIMAEMGVRNAFIPPTALRLMRSVADPRSKYDLVLRTIGSAGEALGRE
TYEWAQRTLGITVNEFYGQTECNFVLSSSAAYGVTRAGAIGRAVPGHRVAIVSEAGDELPAGEPGQIAIASPDPVMFLGY
WDDAAATARKFAKGWLLTGDIGRQDADGYVTFEGRDDDVITSSGYRIGPAEIEDCLIGHPAVQLAAAVGKPDAVRTEIVK
AYIVLSPGHQPSEALAADIREWVKTRLSMHEYPREVEFIDALPLTTTGKVIRRLLREKAAAED
>Mature_543_residues
MILPSHNRYDDLYRDFSWRIPEDFNIGRAVSDDWAARAPKRVCLEHFSPDGHHRTLTYRALADRSSAFANALVSLGIKRG
DRVALLLPQSFETVIAHVAIYKTGVIALPLALLFGVEALEYRLKAAGVAAVVTNGFGLERIRQIREHLPMLKHIVSIDGA
SADAAAFGELVDGHSPVFDVVKTGPDDPALMIFTSGTTGPPKGALHGHRVLPGHIPGMQFAHEGFPKAGDKVWTPSDWAW
AGGLLNALLPSLLLGVPVVSSPAQKFDADMAYRIMAEMGVRNAFIPPTALRLMRSVADPRSKYDLVLRTIGSAGEALGRE
TYEWAQRTLGITVNEFYGQTECNFVLSSSAAYGVTRAGAIGRAVPGHRVAIVSEAGDELPAGEPGQIAIASPDPVMFLGY
WDDAAATARKFAKGWLLTGDIGRQDADGYVTFEGRDDDVITSSGYRIGPAEIEDCLIGHPAVQLAAAVGKPDAVRTEIVK
AYIVLSPGHQPSEALAADIREWVKTRLSMHEYPREVEFIDALPLTTTGKVIRRLLREKAAAED

Specific function: Has a role in growth and sporulation on acetate [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157311624, Length=535, Percent_Identity=32.3364485981308, Blast_Score=253, Evalue=4e-67,
Organism=Homo sapiens, GI157311622, Length=535, Percent_Identity=32.3364485981308, Blast_Score=253, Evalue=4e-67,
Organism=Homo sapiens, GI122937307, Length=536, Percent_Identity=32.8358208955224, Blast_Score=251, Evalue=2e-66,
Organism=Homo sapiens, GI58082049, Length=535, Percent_Identity=32.3364485981308, Blast_Score=249, Evalue=6e-66,
Organism=Homo sapiens, GI115511026, Length=540, Percent_Identity=31.4814814814815, Blast_Score=249, Evalue=7e-66,
Organism=Homo sapiens, GI42544132, Length=549, Percent_Identity=31.511839708561, Blast_Score=246, Evalue=6e-65,
Organism=Homo sapiens, GI38505220, Length=539, Percent_Identity=31.9109461966605, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI28416953, Length=587, Percent_Identity=28.4497444633731, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI13375727, Length=523, Percent_Identity=28.4894837476099, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI8923896, Length=619, Percent_Identity=23.7479806138934, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI187761345, Length=531, Percent_Identity=26.9303201506591, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI187761343, Length=531, Percent_Identity=26.9303201506591, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI156151445, Length=542, Percent_Identity=25.830258302583, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI253970489, Length=373, Percent_Identity=28.1501340482574, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI42544134, Length=378, Percent_Identity=25.6613756613757, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI195947378, Length=450, Percent_Identity=24, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI42794756, Length=502, Percent_Identity=24.9003984063745, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI42794760, Length=502, Percent_Identity=24.9003984063745, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI42794758, Length=502, Percent_Identity=24.9003984063745, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI13325057, Length=380, Percent_Identity=27.3684210526316, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1790505, Length=531, Percent_Identity=29.1902071563089, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1788107, Length=523, Percent_Identity=27.7246653919694, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI145693145, Length=508, Percent_Identity=27.1653543307087, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1786529, Length=569, Percent_Identity=27.0650263620387, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI221142682, Length=529, Percent_Identity=26.4650283553875, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789201, Length=512, Percent_Identity=24.4140625, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=23.9766081871345, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1786801, Length=425, Percent_Identity=26.8235294117647, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788595, Length=523, Percent_Identity=24.0917782026769, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17557194, Length=504, Percent_Identity=28.3730158730159, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71982997, Length=545, Percent_Identity=25.6880733944954, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71983001, Length=545, Percent_Identity=25.6880733944954, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=511, Percent_Identity=27.0058708414873, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=505, Percent_Identity=25.5445544554455, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=495, Percent_Identity=26.8686868686869, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=523, Percent_Identity=26.5774378585086, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71994703, Length=525, Percent_Identity=24.952380952381, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=525, Percent_Identity=24.952380952381, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI71994694, Length=525, Percent_Identity=24.952380952381, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=504, Percent_Identity=24.4047619047619, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=508, Percent_Identity=22.8346456692913, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=533, Percent_Identity=25.515947467167, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=376, Percent_Identity=26.063829787234, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323182, Length=576, Percent_Identity=28.125, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319264, Length=576, Percent_Identity=26.5625, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319699, Length=368, Percent_Identity=27.1739130434783, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24667955, Length=602, Percent_Identity=25.0830564784053, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI62472339, Length=539, Percent_Identity=25.7884972170686, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI21355225, Length=547, Percent_Identity=28.1535648994516, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24667959, Length=371, Percent_Identity=28.0323450134771, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI21355181, Length=531, Percent_Identity=25.4237288135593, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24581924, Length=524, Percent_Identity=24.8091603053435, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859661, Length=511, Percent_Identity=25.2446183953033, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922652, Length=505, Percent_Identity=24.950495049505, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356947, Length=366, Percent_Identity=25.6830601092896, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648260, Length=363, Percent_Identity=25.3443526170799, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24656500, Length=510, Percent_Identity=24.3137254901961, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653035, Length=363, Percent_Identity=24.2424242424242, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648253, Length=464, Percent_Identity=21.7672413793103, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648255, Length=464, Percent_Identity=21.9827586206897, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI161076582, Length=375, Percent_Identity=23.7333333333333, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI20130357, Length=414, Percent_Identity=24.8792270531401, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281365686, Length=333, Percent_Identity=22.8228228228228, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356441, Length=464, Percent_Identity=21.3362068965517, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 58754; Mature: 58754

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPSHNRYDDLYRDFSWRIPEDFNIGRAVSDDWAARAPKRVCLEHFSPDGHHRTLTYRA
CCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCCEEEEEEHH
LADRSSAFANALVSLGIKRGDRVALLLPQSFETVIAHVAIYKTGVIALPLALLFGVEALE
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YRLKAAGVAAVVTNGFGLERIRQIREHLPMLKHIVSIDGASADAAAFGELVDGHSPVFDV
HHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHH
VKTGPDDPALMIFTSGTTGPPKGALHGHRVLPGHIPGMQFAHEGFPKAGDKVWTPSDWAW
HHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHH
AGGLLNALLPSLLLGVPVVSSPAQKFDADMAYRIMAEMGVRNAFIPPTALRLMRSVADPR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCH
SKYDLVLRTIGSAGEALGRETYEWAQRTLGITVNEFYGQTECNFVLSSSAAYGVTRAGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEECCCCCCCHHCCCC
GRAVPGHRVAIVSEAGDELPAGEPGQIAIASPDPVMFLGYWDDAAATARKFAKGWLLTGD
CCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHCCCEEECC
IGRQDADGYVTFEGRDDDVITSSGYRIGPAEIEDCLIGHPAVQLAAAVGKPDAVRTEIVK
CCCCCCCCEEEEECCCCCEEECCCCEECHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHEE
AYIVLSPGHQPSEALAADIREWVKTRLSMHEYPREVEFIDALPLTTTGKVIRRLLREKAA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHC
AED
CCC
>Mature Secondary Structure
MILPSHNRYDDLYRDFSWRIPEDFNIGRAVSDDWAARAPKRVCLEHFSPDGHHRTLTYRA
CCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCCEEEEEEHH
LADRSSAFANALVSLGIKRGDRVALLLPQSFETVIAHVAIYKTGVIALPLALLFGVEALE
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
YRLKAAGVAAVVTNGFGLERIRQIREHLPMLKHIVSIDGASADAAAFGELVDGHSPVFDV
HHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHH
VKTGPDDPALMIFTSGTTGPPKGALHGHRVLPGHIPGMQFAHEGFPKAGDKVWTPSDWAW
HHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHH
AGGLLNALLPSLLLGVPVVSSPAQKFDADMAYRIMAEMGVRNAFIPPTALRLMRSVADPR
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCH
SKYDLVLRTIGSAGEALGRETYEWAQRTLGITVNEFYGQTECNFVLSSSAAYGVTRAGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEECCCCCCCHHCCCC
GRAVPGHRVAIVSEAGDELPAGEPGQIAIASPDPVMFLGYWDDAAATARKFAKGWLLTGD
CCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHCCCEEECC
IGRQDADGYVTFEGRDDDVITSSGYRIGPAEIEDCLIGHPAVQLAAAVGKPDAVRTEIVK
CCCCCCCCEEEEECCCCCEEECCCCEECHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHEE
AYIVLSPGHQPSEALAADIREWVKTRLSMHEYPREVEFIDALPLTTTGKVIRRLLREKAA
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHC
AED
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934817; 9387221; 9384377 [H]