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The map label for this gene is nrdE [H]

Identifier: 209551074

GI number: 209551074

Start: 3615231

End: 3617435

Strand: Reverse

Name: nrdE [H]

Synonym: Rleg2_3498

Alternate gene names: 209551074

Gene position: 3617435-3615231 (Counterclockwise)

Preceding gene: 209551075

Following gene: 209551073

Centisome position: 79.72

GC content: 57.87

Gene sequence:

>2205_bases
TTGGACACGGGAACAACTCTGACGCGGGATGCGGGCGCAAAATCGCATAACAGTTTTGCTCATCCCGCCGGCGAACGCCC
TTTGAAAGCGGCGGAAGCGCTCGATTATCACGCGCTGAACGCCATGCTGAACCTCTATGACGATGAGGGCAGGATCCAGC
TCGACAAGGACCGCATGGCGGCGAAGCAGTATTTCCTGCAGCATGTGAACCAGAACACCGTGTTCTTTCACAATCTGAGG
GAAAAGCTCGATTACCTCGTGACCGAGGGCTATTACGAGCAGGAGGTTCTCGACCAATATTCCTTCAATTTCGTCCGCGA
CCTGTTCGACCAGGCCTATGCCAGGAAATTCCGTTTTCCGACTTTCCTCGGCGCCTTCAAATATTACACCAGCTATACGC
TGAAGACCTTCGACGGAAAGCGCTATCTCGAGCGCTACGAGGATCGCATCTGCATGGTCGCGCTGAGTTTGGCGCGTGGC
GACGAGGCCCTTGCCCGCGACATGGTCGACGAGATCATTTCCGGCCGTTTGCAGCCGGCGACGCCGACTTTCCTCAATGC
CGGCAAGAAACAGCGCGGCGAACTCGTCTCCTGCTTCCTGCTGCGGGTCGAGGACAATATGGAATCGATCGGCCGCTCGA
TCAATTCGGCGCTGCAGCTGTCCAAGCGCGGTGGCGGCGTAGCGCTGTCGCTGACGAACATCCGCGAGGCCAGCGCGCCG
ATCAAGCAGATCGAGAACCAGTCGTCGGGCATCATCCCGGTGATGAAGCTGCTCGAAGACAGTTTCTCCTATGCCAACCA
GCTCGGCGCCCGCCAGGGGGCGGGTGCCGTCTATCTCAACGCCCATCACCCCGATATCATGCGCTTCCTCGACACCAAGC
GCGAGAATGCAGACGAGAAGATCCGCATCAAGACGCTATCGCTCGGCGTCGTCGTGCCCGACATCAGCTTCGAACTCGCG
AGGAATAACGAGGACATGTACCTGTTCTCGCCCTATGACGTGGAGCGCATCTATGGGGTGCCCTTTACCGAGATTTCGGT
GACGGAAAAGTATCGGGAGATGGTGGCGGACGCCCGTATCTCGAAGAAGAAGATCAAGGCGCGCGAATTCTTCCAGGTGA
TCGCCGAAATCCAGTTCGAGAGCGGTTATCCCTACATCATGTTCGAGGACACGGTGAACCGGGCGAACCCGATTGCCGGG
CGGATTTCAATGAGCAATCTCTGCTCGGAGATCCTGCAGGTCAGCGAGGCGAGCGAATACAATGACGACCTCTCCTACAA
ACATCTCGGCAAGGATATTTCCTGCAATCTCGGCTCGCTGAACATTGCCGCAGCCATGGATTCCGCCGATTTCGGCAAGA
CGATCGAGACCTCGATCCGGGCGCTGACGGCGGTCTCCGACATGAGCCACATCGCCTCGGTTCCCTCGATCGAAAAGGGC
AACGACGAGAGCCACGCGATCGGCCTCGGCCAGATGAACCTGCACGGATACCTCGCCCGCGAACGTATCTTTTACGGTTC
CGAGGAAGGCGTCGATTTCACCAACATCTATTTCTATACGGTGACCTATCACGCGATCCGCGCCTCGAACCTGTTGGCGG
TCGAGCGCGGCACAAGCTTCAAGGGGTTCGAGAACTCGAAATATGCCTCCGGCGAATATTTCGACAAATATACCGAGCAG
GAATGGCAGCCGGCGACGGAGAAGGTGCGGGCGCTGTTCGATAATGCCGGCATCCCTGTTCCGACACAGGAAGACTGGTT
GGCGCTGAAGACGGCTGTCATGACCTCCGGTCTCTATAACCAGAATCTGCAGGCGGTGCCGCCGACCGGGTCGATCTCCT
ATATCAACCACTCGACCTCCTCGATCCATCCGATCGTTTCGAAGATCGAGATCCGCAAGGAAGGCAAGATCGGCCGCGTC
TATTATCCGGCGCCGTTCATGACCAACGACAATCTCGATTATTACCAGGATGCCTACGAGATCGGGCCGGAGAAGATTAT
CGACACCTATGCGGCGGCGACCCAGCATGTCGACCAGGGCCTGTCGCTAACCTTGTTCTTCCGCGACACGGCGACGACGC
GCGACATCAACAAGGCGCAGATCTACGCCTGGAAGAAGGGCATCAAGACGATCTACTACATCCGCCTTCGCCAGATGGCG
CTGTCCGGCACCGAGGTGCAGGGCTGCGTGTCTTGCACGCTGTAA

Upstream 100 bases:

>100_bases
ACGTGATCTCGCAGAAATGCCGGGTGCCGTACCTCTACAGGTTCGAGCTCATCGGCACGGAGGAGGATGTCGCCAACGTC
AAACACGGAATGGAACGATT

Downstream 100 bases:

>100_bases
CCGATCGGGGAACATCATGAACATGCAGATCAAACCGGTGTCCCGCGTGCGTGCCATCAACTGGAACCGCATCGAGGACG
ACAAGGATCTCGAAGTCTGG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 734; Mature: 734

Protein sequence:

>734_residues
MDTGTTLTRDAGAKSHNSFAHPAGERPLKAAEALDYHALNAMLNLYDDEGRIQLDKDRMAAKQYFLQHVNQNTVFFHNLR
EKLDYLVTEGYYEQEVLDQYSFNFVRDLFDQAYARKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALSLARG
DEALARDMVDEIISGRLQPATPTFLNAGKKQRGELVSCFLLRVEDNMESIGRSINSALQLSKRGGGVALSLTNIREASAP
IKQIENQSSGIIPVMKLLEDSFSYANQLGARQGAGAVYLNAHHPDIMRFLDTKRENADEKIRIKTLSLGVVVPDISFELA
RNNEDMYLFSPYDVERIYGVPFTEISVTEKYREMVADARISKKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPIAG
RISMSNLCSEILQVSEASEYNDDLSYKHLGKDISCNLGSLNIAAAMDSADFGKTIETSIRALTAVSDMSHIASVPSIEKG
NDESHAIGLGQMNLHGYLARERIFYGSEEGVDFTNIYFYTVTYHAIRASNLLAVERGTSFKGFENSKYASGEYFDKYTEQ
EWQPATEKVRALFDNAGIPVPTQEDWLALKTAVMTSGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRV
YYPAPFMTNDNLDYYQDAYEIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMA
LSGTEVQGCVSCTL

Sequences:

>Translated_734_residues
MDTGTTLTRDAGAKSHNSFAHPAGERPLKAAEALDYHALNAMLNLYDDEGRIQLDKDRMAAKQYFLQHVNQNTVFFHNLR
EKLDYLVTEGYYEQEVLDQYSFNFVRDLFDQAYARKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALSLARG
DEALARDMVDEIISGRLQPATPTFLNAGKKQRGELVSCFLLRVEDNMESIGRSINSALQLSKRGGGVALSLTNIREASAP
IKQIENQSSGIIPVMKLLEDSFSYANQLGARQGAGAVYLNAHHPDIMRFLDTKRENADEKIRIKTLSLGVVVPDISFELA
RNNEDMYLFSPYDVERIYGVPFTEISVTEKYREMVADARISKKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPIAG
RISMSNLCSEILQVSEASEYNDDLSYKHLGKDISCNLGSLNIAAAMDSADFGKTIETSIRALTAVSDMSHIASVPSIEKG
NDESHAIGLGQMNLHGYLARERIFYGSEEGVDFTNIYFYTVTYHAIRASNLLAVERGTSFKGFENSKYASGEYFDKYTEQ
EWQPATEKVRALFDNAGIPVPTQEDWLALKTAVMTSGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRV
YYPAPFMTNDNLDYYQDAYEIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMA
LSGTEVQGCVSCTL
>Mature_734_residues
MDTGTTLTRDAGAKSHNSFAHPAGERPLKAAEALDYHALNAMLNLYDDEGRIQLDKDRMAAKQYFLQHVNQNTVFFHNLR
EKLDYLVTEGYYEQEVLDQYSFNFVRDLFDQAYARKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALSLARG
DEALARDMVDEIISGRLQPATPTFLNAGKKQRGELVSCFLLRVEDNMESIGRSINSALQLSKRGGGVALSLTNIREASAP
IKQIENQSSGIIPVMKLLEDSFSYANQLGARQGAGAVYLNAHHPDIMRFLDTKRENADEKIRIKTLSLGVVVPDISFELA
RNNEDMYLFSPYDVERIYGVPFTEISVTEKYREMVADARISKKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPIAG
RISMSNLCSEILQVSEASEYNDDLSYKHLGKDISCNLGSLNIAAAMDSADFGKTIETSIRALTAVSDMSHIASVPSIEKG
NDESHAIGLGQMNLHGYLARERIFYGSEEGVDFTNIYFYTVTYHAIRASNLLAVERGTSFKGFENSKYASGEYFDKYTEQ
EWQPATEKVRALFDNAGIPVPTQEDWLALKTAVMTSGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRV
YYPAPFMTNDNLDYYQDAYEIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMA
LSGTEVQGCVSCTL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=656, Percent_Identity=25.9146341463415, Blast_Score=184, Evalue=3e-46,
Organism=Escherichia coli, GI1789030, Length=703, Percent_Identity=73.1152204836415, Blast_Score=1103, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=568, Percent_Identity=25.5281690140845, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17555242, Length=651, Percent_Identity=26.7281105990783, Blast_Score=185, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6320914, Length=654, Percent_Identity=26.2996941896024, Blast_Score=183, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6322123, Length=663, Percent_Identity=26.0935143288084, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17352481, Length=673, Percent_Identity=25.55720653789, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 82856; Mature: 82856

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGTTLTRDAGAKSHNSFAHPAGERPLKAAEALDYHALNAMLNLYDDEGRIQLDKDRMA
CCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHH
AKQYFLQHVNQNTVFFHNLREKLDYLVTEGYYEQEVLDQYSFNFVRDLFDQAYARKFRFP
HHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TFLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALSLARGDEALARDMVDEIISGRLQPA
HHHHHHHHHHHEEEEECCCHHHHHHHHHHHEEEHHHHHCCHHHHHHHHHHHHHCCCCCCC
TPTFLNAGKKQRGELVSCFLLRVEDNMESIGRSINSALQLSKRGGGVALSLTNIREASAP
CCHHHCCCCHHHCCCEEHEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHCCH
IKQIENQSSGIIPVMKLLEDSFSYANQLGARQGAGAVYLNAHHPDIMRFLDTKRENADEK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCE
IRIKTLSLGVVVPDISFELARNNEDMYLFSPYDVERIYGVPFTEISVTEKYREMVADARI
EEEEEEEEEEEECCCEEEEECCCCCEEEECCCCCHHCCCCCCCEEHHHHHHHHHHHHHHH
SKKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPIAGRISMSNLCSEILQVSEASEY
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
NDDLSYKHLGKDISCNLGSLNIAAAMDSADFGKTIETSIRALTAVSDMSHIASVPSIEKG
CCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
NDESHAIGLGQMNLHGYLARERIFYGSEEGVDFTNIYFYTVTYHAIRASNLLAVERGTSF
CCCCCEEEECCCCHHHHHHHHHEEECCCCCCCEEEEEEEEEEEEEHHHCCEEEEECCCCC
KGFENSKYASGEYFDKYTEQEWQPATEKVRALFDNAGIPVPTQEDWLALKTAVMTSGLYN
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC
QNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPAPFMTNDNLDYYQDAYE
CCCEECCCCCCEEEEECCCCHHHHHHHHHHEECCCCCCEEEECCCCCCCCCCHHHHHHHH
IGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMA
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEHHHHHHHH
LSGTEVQGCVSCTL
CCCCCCCCEEEECC
>Mature Secondary Structure
MDTGTTLTRDAGAKSHNSFAHPAGERPLKAAEALDYHALNAMLNLYDDEGRIQLDKDRMA
CCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHH
AKQYFLQHVNQNTVFFHNLREKLDYLVTEGYYEQEVLDQYSFNFVRDLFDQAYARKFRFP
HHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TFLGAFKYYTSYTLKTFDGKRYLERYEDRICMVALSLARGDEALARDMVDEIISGRLQPA
HHHHHHHHHHHEEEEECCCHHHHHHHHHHHEEEHHHHHCCHHHHHHHHHHHHHCCCCCCC
TPTFLNAGKKQRGELVSCFLLRVEDNMESIGRSINSALQLSKRGGGVALSLTNIREASAP
CCHHHCCCCHHHCCCEEHEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHCCH
IKQIENQSSGIIPVMKLLEDSFSYANQLGARQGAGAVYLNAHHPDIMRFLDTKRENADEK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCE
IRIKTLSLGVVVPDISFELARNNEDMYLFSPYDVERIYGVPFTEISVTEKYREMVADARI
EEEEEEEEEEEECCCEEEEECCCCCEEEECCCCCHHCCCCCCCEEHHHHHHHHHHHHHHH
SKKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPIAGRISMSNLCSEILQVSEASEY
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
NDDLSYKHLGKDISCNLGSLNIAAAMDSADFGKTIETSIRALTAVSDMSHIASVPSIEKG
CCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
NDESHAIGLGQMNLHGYLARERIFYGSEEGVDFTNIYFYTVTYHAIRASNLLAVERGTSF
CCCCCEEEECCCCHHHHHHHHHEEECCCCCCCEEEEEEEEEEEEEHHHCCEEEEECCCCC
KGFENSKYASGEYFDKYTEQEWQPATEKVRALFDNAGIPVPTQEDWLALKTAVMTSGLYN
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCC
QNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPAPFMTNDNLDYYQDAYE
CCCEECCCCCCEEEEECCCCHHHHHHHHHHEECCCCCCEEEECCCCCCCCCCHHHHHHHH
IGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINKAQIYAWKKGIKTIYYIRLRQMA
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEHHHHHHHH
LSGTEVQGCVSCTL
CCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]