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The map label for this gene is lacZ [H]

Identifier: 209551063

GI number: 209551063

Start: 3603007

End: 3605253

Strand: Direct

Name: lacZ [H]

Synonym: Rleg2_3487

Alternate gene names: 209551063

Gene position: 3603007-3605253 (Clockwise)

Preceding gene: 209551062

Following gene: 209551068

Centisome position: 79.4

GC content: 62.35

Gene sequence:

>2247_bases
ATGCGTTCCGTCGTTTCCTTCAACGAAGGCTGGAGTTTCCATGAGGGCTTCGGACAGCGTCTGCTCGAAAGCTTCGACGC
CGCCAAGTCGGTCAGCCTGCCCCATACCGCCGTCGAACTGCCTTTCAACTATTTCGACGAAAAGAGCTATCAGCGCGCCT
TCACCTATCAAAAGGTGCTGCGCTGGCTGCCGGAATTCGAGGGCCGCGAGGTGTCGCTCGTCTTCGACGCCGCCATGGCC
GACAGCGTCGTCTACCTCAACGGCGAAGAGATCATCGCCCATAAGGACGGCTACACCCCCTTCGAGGCTCGCCTCACCGG
CAAGCTCGTCAAGGGCGAGAACCTGGTGACGGTCAAGATCGACGGCAGCGAAAACCCTGAGATCCCGCCCTTCGGCGGCC
GCATCGATTATCTGACTTATGCCGGCATCTACCGCGACGTCTGGCTGAAGGTCACCGACCCCGTCTCGATCCGCAATCTC
AAGATCGAAACCGCAGACGTTCTTGCCCCGGAAAAATCGGCAACCATCCGTGTCGATATCGCCAACCCCGATGGCCGCAG
CTTCTCGGCAACCGTCACCGCCACGCTGAAGCAGGCAGACGGCACGGTGATCGCCACCGCAGCAACCGAGACGATCGGCG
ACCGCACCAGGCTTTCTTTCGGCGGCCTCACCGGCATCACGCTCTGGGACATTACCGATCCCACCCTCTACGACGTCACC
GTCGAACTCAGGACCGAGCACGGCTCCGACCGTATCTCCACCCGCTTCGGCTTCCGCACCGCTGAATTCACCCCGGAAGG
TTTCCTTCTCAACGGCAAGCCGCTGAAGCTGCGCGGCCTCAACCGCCACCAGTCCTTCCCCTATGTCGGTTATGCCGCCG
GCCGCTCCGCCCAGGAGCGTGACGCCGACATCATGAAGACGGTGCTGAAGTGCAATATCGTCCGCACCTCGCATTATCCG
CAATCGAAATGGTTCCTCGACCGCTGCGACGCCATCGGCCTGCTGGTTTTCGAGGAAATCCCCGGCTGGCAGCATATCGG
CGATGCCGACTGGCAGAAGGAATCGATCGAAAACGTCCGCCGGATGATCGAGCGCGACTGGAACCATCCCTCGATCATCA
TCTGGGGCGTGCGCATCAACGAGTCCCAGGATAGTCACGATTTCTACGTCGAGACCAATCGCCTCGCCCGTGAGCTCGAC
AGCACCCGCCAGACCGGCGGCGTGCGTTACATCACCGAGAGCGAGCTGCTCGAAGACGTCTACACGATGAATGACTTCAT
CCTCGGCAACGAGGAGCTGCCGGGCGCCAACCGCCCGCGCACCGCATTGCGCGGCCAGGAGGAAAATACCGGTCTGTCCC
GGAAGGTGCCGTACCTCATCACCGAGTTCAACGGCCACATGCATCCGACGAAAATCTACGACCAGGAGCAGCGCCAGGCC
GAGCATGTGCGCCGTCACCTGGAGGTGCTGAATGCCGCCTATGGCGATCCCGATATCTCCGGCGCCATCGGCTGGTGCAT
GTTCGATTACAACACCCACAAGGATTTCGGCTCCGGCGACCGCATCTGCTATCACGGCGTCATGGACATGTTCCGCGAAC
CGAAATTCGCGGCCTATGCCTATATCAGCCAGTGCGACCCGTCCGACGAGATCGTCATGAAGCCGGTGACCTTCTGGGCC
CGCGGCGAGCGCAATATCGGCGGCGTGCTGCCGCTGATCATCCTGACCAATTGCGACGAGGTGGAACTGCAATATGGCGG
GCTCAGCAAGCGCATCGGCCCGGACCGCGAGAACTATCCGCATCTGCCGCATCCGCCTGTCGTGCTCGATCATCGCCACT
TCACCGCCGATGAACTCGGCACCTGGGGTCTGGAATGGATCGACGGCACCTTGACCGGCTATATCGGCGGCGAGCCGGTG
ACGAGCCTGACCTTTGCCGCCGATCCCTTGCCGACGACGCTGGAAATCGTCGCCGACAGCCACACGCTGAGGGCGCGCGA
ACGCGACAGCACCCGCGTCATCATTCGCGCCCTCGACCAGTGCGGCCAGCGCCTGCCATTCATGAACGACAGCATTTCGC
TGAAGGTGCATGGGCCCGCAAAGATCGTCGGCCCGGCCAATGTGCCGCTACAGGGCGGTACCGCGGGCTTCTGGCTGGAG
GCGACGGGGATGGTCGGCGAGATCACGATCGAAGCGGTCTCCTCGCGTTTCCCCCCGGTGACCCTCAATGTGACGGCCGC
CGCCTGA

Upstream 100 bases:

>100_bases
CTCGCAGCTTATTGCATCAAGGAGGAATACAAAATGACCCGATTTTCACGCGCCGAAAGGCGCCGCACCGCTCCTGATGC
CGTGATCGGGAGAGCCTGAG

Downstream 100 bases:

>100_bases
CGGCTGTCACGAAATCCTGAGCTGGCTCTCAGGATCGAAGAACACGGCCTTGTCGAGATTGAAGGCAAGGCGTGTGTTTT
GTCCGGGGGCGATGCCGGCA

Product: glycoside hydrolase family 2 TIM barrel

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MRSVVSFNEGWSFHEGFGQRLLESFDAAKSVSLPHTAVELPFNYFDEKSYQRAFTYQKVLRWLPEFEGREVSLVFDAAMA
DSVVYLNGEEIIAHKDGYTPFEARLTGKLVKGENLVTVKIDGSENPEIPPFGGRIDYLTYAGIYRDVWLKVTDPVSIRNL
KIETADVLAPEKSATIRVDIANPDGRSFSATVTATLKQADGTVIATAATETIGDRTRLSFGGLTGITLWDITDPTLYDVT
VELRTEHGSDRISTRFGFRTAEFTPEGFLLNGKPLKLRGLNRHQSFPYVGYAAGRSAQERDADIMKTVLKCNIVRTSHYP
QSKWFLDRCDAIGLLVFEEIPGWQHIGDADWQKESIENVRRMIERDWNHPSIIIWGVRINESQDSHDFYVETNRLARELD
STRQTGGVRYITESELLEDVYTMNDFILGNEELPGANRPRTALRGQEENTGLSRKVPYLITEFNGHMHPTKIYDQEQRQA
EHVRRHLEVLNAAYGDPDISGAIGWCMFDYNTHKDFGSGDRICYHGVMDMFREPKFAAYAYISQCDPSDEIVMKPVTFWA
RGERNIGGVLPLIILTNCDEVELQYGGLSKRIGPDRENYPHLPHPPVVLDHRHFTADELGTWGLEWIDGTLTGYIGGEPV
TSLTFAADPLPTTLEIVADSHTLRARERDSTRVIIRALDQCGQRLPFMNDSISLKVHGPAKIVGPANVPLQGGTAGFWLE
ATGMVGEITIEAVSSRFPPVTLNVTAAA

Sequences:

>Translated_748_residues
MRSVVSFNEGWSFHEGFGQRLLESFDAAKSVSLPHTAVELPFNYFDEKSYQRAFTYQKVLRWLPEFEGREVSLVFDAAMA
DSVVYLNGEEIIAHKDGYTPFEARLTGKLVKGENLVTVKIDGSENPEIPPFGGRIDYLTYAGIYRDVWLKVTDPVSIRNL
KIETADVLAPEKSATIRVDIANPDGRSFSATVTATLKQADGTVIATAATETIGDRTRLSFGGLTGITLWDITDPTLYDVT
VELRTEHGSDRISTRFGFRTAEFTPEGFLLNGKPLKLRGLNRHQSFPYVGYAAGRSAQERDADIMKTVLKCNIVRTSHYP
QSKWFLDRCDAIGLLVFEEIPGWQHIGDADWQKESIENVRRMIERDWNHPSIIIWGVRINESQDSHDFYVETNRLARELD
STRQTGGVRYITESELLEDVYTMNDFILGNEELPGANRPRTALRGQEENTGLSRKVPYLITEFNGHMHPTKIYDQEQRQA
EHVRRHLEVLNAAYGDPDISGAIGWCMFDYNTHKDFGSGDRICYHGVMDMFREPKFAAYAYISQCDPSDEIVMKPVTFWA
RGERNIGGVLPLIILTNCDEVELQYGGLSKRIGPDRENYPHLPHPPVVLDHRHFTADELGTWGLEWIDGTLTGYIGGEPV
TSLTFAADPLPTTLEIVADSHTLRARERDSTRVIIRALDQCGQRLPFMNDSISLKVHGPAKIVGPANVPLQGGTAGFWLE
ATGMVGEITIEAVSSRFPPVTLNVTAAA
>Mature_748_residues
MRSVVSFNEGWSFHEGFGQRLLESFDAAKSVSLPHTAVELPFNYFDEKSYQRAFTYQKVLRWLPEFEGREVSLVFDAAMA
DSVVYLNGEEIIAHKDGYTPFEARLTGKLVKGENLVTVKIDGSENPEIPPFGGRIDYLTYAGIYRDVWLKVTDPVSIRNL
KIETADVLAPEKSATIRVDIANPDGRSFSATVTATLKQADGTVIATAATETIGDRTRLSFGGLTGITLWDITDPTLYDVT
VELRTEHGSDRISTRFGFRTAEFTPEGFLLNGKPLKLRGLNRHQSFPYVGYAAGRSAQERDADIMKTVLKCNIVRTSHYP
QSKWFLDRCDAIGLLVFEEIPGWQHIGDADWQKESIENVRRMIERDWNHPSIIIWGVRINESQDSHDFYVETNRLARELD
STRQTGGVRYITESELLEDVYTMNDFILGNEELPGANRPRTALRGQEENTGLSRKVPYLITEFNGHMHPTKIYDQEQRQA
EHVRRHLEVLNAAYGDPDISGAIGWCMFDYNTHKDFGSGDRICYHGVMDMFREPKFAAYAYISQCDPSDEIVMKPVTFWA
RGERNIGGVLPLIILTNCDEVELQYGGLSKRIGPDRENYPHLPHPPVVLDHRHFTADELGTWGLEWIDGTLTGYIGGEPV
TSLTFAADPLPTTLEIVADSHTLRARERDSTRVIIRALDQCGQRLPFMNDSISLKVHGPAKIVGPANVPLQGGTAGFWLE
ATGMVGEITIEAVSSRFPPVTLNVTAAA

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=539, Percent_Identity=24.1187384044527, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1787903, Length=449, Percent_Identity=24.7216035634744, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI48994920, Length=343, Percent_Identity=27.4052478134111, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1786539, Length=480, Percent_Identity=27.5, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17510825, Length=530, Percent_Identity=24.5283018867925, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922582, Length=517, Percent_Identity=24.3713733075435, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI62471735, Length=517, Percent_Identity=24.3713733075435, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24655438, Length=517, Percent_Identity=24.3713733075435, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24651745, Length=513, Percent_Identity=25.1461988304094, Blast_Score=109, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 83901; Mature: 83901

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1 ; PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVVSFNEGWSFHEGFGQRLLESFDAAKSVSLPHTAVELPFNYFDEKSYQRAFTYQKVL
CCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHCCCHHHHHHHHHHHHH
RWLPEFEGREVSLVFDAAMADSVVYLNGEEIIAHKDGYTPFEARLTGKLVKGENLVTVKI
HHCCCCCCCEEEEEEECCCCCCEEEECCCEEEEECCCCCCEEEEEEEEEECCCCEEEEEE
DGSENPEIPPFGGRIDYLTYAGIYRDVWLKVTDPVSIRNLKIETADVLAPEKSATIRVDI
CCCCCCCCCCCCCEECEEEECCEEEEEEEEEECCEEEEEEEEEHHHHCCCCCCCEEEEEE
ANPDGRSFSATVTATLKQADGTVIATAATETIGDRTRLSFGGLTGITLWDITDPTLYDVT
CCCCCCCEEEEEEEEEECCCCCEEEEECHHHCCCCCEEEECCCCEEEEEECCCCEEEEEE
VELRTEHGSDRISTRFGFRTAEFTPEGFLLNGKPLKLRGLNRHQSFPYVGYAAGRSAQER
EEEEECCCCCCHHHCCCCEEECCCCCCEEECCCCEEEECCCCCCCCCEEEEECCCCCCHH
DADIMKTVLKCNIVRTSHYPQSKWFLDRCDAIGLLVFEEIPGWQHIGDADWQKESIENVR
HHHHHHHHHHEEEEEECCCCCCCHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHH
RMIERDWNHPSIIIWGVRINESQDSHDFYVETNRLARELDSTRQTGGVRYITESELLEDV
HHHHHCCCCCEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
YTMNDFILGNEELPGANRPRTALRGQEENTGLSRKVPYLITEFNGHMHPTKIYDQEQRQA
HHHHHEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCHHHHHH
EHVRRHLEVLNAAYGDPDISGAIGWCMFDYNTHKDFGSGDRICYHGVMDMFREPKFAAYA
HHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHCCCCCEEEE
YISQCDPSDEIVMKPVTFWARGERNIGGVLPLIILTNCDEVELQYGGLSKRIGPDRENYP
EECCCCCCCCEEEEEEEEEECCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCC
HLPHPPVVLDHRHFTADELGTWGLEWIDGTLTGYIGGEPVTSLTFAADPLPTTLEIVADS
CCCCCCEEECCCCCCHHHHCCCCCEEECCEEEEEECCCCCCEEEEECCCCCCEEEEEECC
HTLRARERDSTRVIIRALDQCGQRLPFMNDSISLKVHGPAKIVGPANVPLQGGTAGFWLE
CEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCEEEEE
ATGMVGEITIEAVSSRFPPVTLNVTAAA
ECCCEEEEEEEEHHCCCCCEEEEEEECC
>Mature Secondary Structure
MRSVVSFNEGWSFHEGFGQRLLESFDAAKSVSLPHTAVELPFNYFDEKSYQRAFTYQKVL
CCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHCCCHHHHHHHHHHHHH
RWLPEFEGREVSLVFDAAMADSVVYLNGEEIIAHKDGYTPFEARLTGKLVKGENLVTVKI
HHCCCCCCCEEEEEEECCCCCCEEEECCCEEEEECCCCCCEEEEEEEEEECCCCEEEEEE
DGSENPEIPPFGGRIDYLTYAGIYRDVWLKVTDPVSIRNLKIETADVLAPEKSATIRVDI
CCCCCCCCCCCCCEECEEEECCEEEEEEEEEECCEEEEEEEEEHHHHCCCCCCCEEEEEE
ANPDGRSFSATVTATLKQADGTVIATAATETIGDRTRLSFGGLTGITLWDITDPTLYDVT
CCCCCCCEEEEEEEEEECCCCCEEEEECHHHCCCCCEEEECCCCEEEEEECCCCEEEEEE
VELRTEHGSDRISTRFGFRTAEFTPEGFLLNGKPLKLRGLNRHQSFPYVGYAAGRSAQER
EEEEECCCCCCHHHCCCCEEECCCCCCEEECCCCEEEECCCCCCCCCEEEEECCCCCCHH
DADIMKTVLKCNIVRTSHYPQSKWFLDRCDAIGLLVFEEIPGWQHIGDADWQKESIENVR
HHHHHHHHHHEEEEEECCCCCCCHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHH
RMIERDWNHPSIIIWGVRINESQDSHDFYVETNRLARELDSTRQTGGVRYITESELLEDV
HHHHHCCCCCEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
YTMNDFILGNEELPGANRPRTALRGQEENTGLSRKVPYLITEFNGHMHPTKIYDQEQRQA
HHHHHEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCHHHHHH
EHVRRHLEVLNAAYGDPDISGAIGWCMFDYNTHKDFGSGDRICYHGVMDMFREPKFAAYA
HHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHCCCCCEEEE
YISQCDPSDEIVMKPVTFWARGERNIGGVLPLIILTNCDEVELQYGGLSKRIGPDRENYP
EECCCCCCCCEEEEEEEEEECCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCC
HLPHPPVVLDHRHFTADELGTWGLEWIDGTLTGYIGGEPVTSLTFAADPLPTTLEIVADS
CCCCCCEEECCCCCCHHHHCCCCCEEECCEEEEEECCCCCCEEEEECCCCCCEEEEEECC
HTLRARERDSTRVIIRALDQCGQRLPFMNDSISLKVHGPAKIVGPANVPLQGGTAGFWLE
CEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCEEEEE
ATGMVGEITIEAVSSRFPPVTLNVTAAA
ECCCEEEEEEEEHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8163182 [H]