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The map label for this gene is nth [H]

Identifier: 209551017

GI number: 209551017

Start: 3555975

End: 3556757

Strand: Direct

Name: nth [H]

Synonym: Rleg2_3441

Alternate gene names: 209551017

Gene position: 3555975-3556757 (Clockwise)

Preceding gene: 209551014

Following gene: 209551019

Centisome position: 78.36

GC content: 63.22

Gene sequence:

>783_bases
ATGGCGAATCCGAAACTCAAATCCGTGACCAGACCGTCGCAAAACTCGAATGTGATCGCTCGCAGCAAACCGGCGACGGC
GGTGAAGACCGCCTATTCGCTCGCCGAGCGTGAGGAGATCTTCCGCCGCTTCTCGGTGCAGCGGCCGGAGCCGAGGGGCG
AACTTGAGCATAGCAATCCCTTCACCCTCGTCGTCGCCGTCGCACTGTCGGCGCAGGCGACCGATGTCGGCGTCAACAAG
GCGACCCGCGCCCTCTTCAAGGTTGCCGATACGCCGGAAAAGATGCTCGATCTCGGCGAGGAGCGGCTGCGCGACTATAT
CAGGACGATCGGCCTCTATCGCAACAAGGCGAAAAACGTCATCGCCCTCTCGCAGATGCTGGTCGATGATTTCGCCGGCA
AGGTGCCGGAGACGCGCGACGAACTGGTGAAACTGCCGGGCGTCGGCCGCAAGACCGCCAATGTCGTGCTCTCCATGGCC
TTCGGCCAGGCGACGATGGCCGTCGACACCCATATCTTCCGCATCGCCAATCGCATCAAGCTTGCCCCCGGCAAGACACC
CGACGAGGTCGAGGCGCGGCTGATGAAGGTGATCCCGCAACATTACCTCTACCATGCCCATCACTGGCTGATCCTGCACG
GCCGCCACACCTGCAAGGCGCGCCGCCCCGAATGTGAACGCTGCGTCATCGCCGATCTCTGCAAGTCGCCGGAAAAGAGT
TGGGATGTGCCGGCCCCGCTCGTCGAGCTACCGCCGCAGGTGATCGGCGAGGCCGTCGAGTAG

Upstream 100 bases:

>100_bases
GCCGAGCGATCGGTGGGGGAAAAGCTCGGCAGCGAAAACAGGCTGCTCGTTGAGGCTGTCGGCGTTGCTTTCCATCATGG
CTGCTGAGTATAGATTCACC

Downstream 100 bases:

>100_bases
AGCAGGTCGCCGCCCTGCCCGGCTGAACCTCACACCCGAAAACCGGCGTCGCGGGCGGAAACCATCTTGTGCAGATGCAC
CATCATGCCGGCGGCAAAGA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MANPKLKSVTRPSQNSNVIARSKPATAVKTAYSLAEREEIFRRFSVQRPEPRGELEHSNPFTLVVAVALSAQATDVGVNK
ATRALFKVADTPEKMLDLGEERLRDYIRTIGLYRNKAKNVIALSQMLVDDFAGKVPETRDELVKLPGVGRKTANVVLSMA
FGQATMAVDTHIFRIANRIKLAPGKTPDEVEARLMKVIPQHYLYHAHHWLILHGRHTCKARRPECERCVIADLCKSPEKS
WDVPAPLVELPPQVIGEAVE

Sequences:

>Translated_260_residues
MANPKLKSVTRPSQNSNVIARSKPATAVKTAYSLAEREEIFRRFSVQRPEPRGELEHSNPFTLVVAVALSAQATDVGVNK
ATRALFKVADTPEKMLDLGEERLRDYIRTIGLYRNKAKNVIALSQMLVDDFAGKVPETRDELVKLPGVGRKTANVVLSMA
FGQATMAVDTHIFRIANRIKLAPGKTPDEVEARLMKVIPQHYLYHAHHWLILHGRHTCKARRPECERCVIADLCKSPEKS
WDVPAPLVELPPQVIGEAVE
>Mature_259_residues
ANPKLKSVTRPSQNSNVIARSKPATAVKTAYSLAEREEIFRRFSVQRPEPRGELEHSNPFTLVVAVALSAQATDVGVNKA
TRALFKVADTPEKMLDLGEERLRDYIRTIGLYRNKAKNVIALSQMLVDDFAGKVPETRDELVKLPGVGRKTANVVLSMAF
GQATMAVDTHIFRIANRIKLAPGKTPDEVEARLMKVIPQHYLYHAHHWLILHGRHTCKARRPECERCVIADLCKSPEKSW
DVPAPLVELPPQVIGEAVE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=56.0975609756098, Blast_Score=242, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.7683615819209, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324530, Length=189, Percent_Identity=26.4550264550265, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319304, Length=135, Percent_Identity=31.8518518518519, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=29.608938547486, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 29029; Mature: 28898

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPKLKSVTRPSQNSNVIARSKPATAVKTAYSLAEREEIFRRFSVQRPEPRGELEHSNP
CCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
FTLVVAVALSAQATDVGVNKATRALFKVADTPEKMLDLGEERLRDYIRTIGLYRNKAKNV
CEEEHHHHHHCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALSQMLVDDFAGKVPETRDELVKLPGVGRKTANVVLSMAFGQATMAVDTHIFRIANRIK
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHE
LAPGKTPDEVEARLMKVIPQHYLYHAHHWLILHGRHTCKARRPECERCVIADLCKSPEKS
ECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCC
WDVPAPLVELPPQVIGEAVE
CCCCCCHHHCCHHHHHHCCC
>Mature Secondary Structure 
ANPKLKSVTRPSQNSNVIARSKPATAVKTAYSLAEREEIFRRFSVQRPEPRGELEHSNP
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
FTLVVAVALSAQATDVGVNKATRALFKVADTPEKMLDLGEERLRDYIRTIGLYRNKAKNV
CEEEHHHHHHCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALSQMLVDDFAGKVPETRDELVKLPGVGRKTANVVLSMAFGQATMAVDTHIFRIANRIK
HHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHE
LAPGKTPDEVEARLMKVIPQHYLYHAHHWLILHGRHTCKARRPECERCVIADLCKSPEKS
ECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCC
WDVPAPLVELPPQVIGEAVE
CCCCCCHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA