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The map label for this gene is yfkN [H]

Identifier: 209550879

GI number: 209550879

Start: 3391128

End: 3393107

Strand: Direct

Name: yfkN [H]

Synonym: Rleg2_3303

Alternate gene names: 209550879

Gene position: 3391128-3393107 (Clockwise)

Preceding gene: 209550878

Following gene: 209550880

Centisome position: 74.73

GC content: 63.13

Gene sequence:

>1980_bases
ATGACGAAGTCTTTCAGCTTCGGTCTTTTGACCGCGTCCATGCTGGCGCTGAGCACGGGCGCCGCTTTTGCGGATTACGA
ACTCAATATTCTTCATATCAACGATTTCCATTCGCGCATCGAATCGATCAACAAGTTCGACTCCACCTGCTCGGCCGAGG
AAGAAGGCAAGAAGGAATGCTTCGGCGGTGCTGCCCGCCTGAAGACCGCGATCGACCAGCGCCGTCAGGCGCTTTCCGGC
AAGAATGTCCTCCTGCTGAATGCCGGCGACAATTTCCAGGGCTCGCTGTTCTACACGACCTACAAGGGCGCGGCCGAAGC
CGAATTCCTCAACCTGATGAAGTTCGACGCCATGACCGTCGGCAACCATGAATTCGACGACAGCGAGGACGGGCTTGCGA
CCTTCCTCGACAAGGTGCAATTCCCCGTCGTTACCGCAAACGTCAAGGCGGCAGCCGCCTCAAAGCTCGGCGACCGCATC
AAGCCCTCGCTGGTGCTCGATGTCGGTGGCCAGAAGATCGGCATCGTCGGCGCCGTCACCAATGACACGGCCGAACTTTC
CTCCCCCGGCCCGAATGTCACGATATCAGATGACGTCCAGGCCATTACGTCAGCCGTTCAGGATCTGAAGGGCCAGGGCG
TCAACAAGATCATCGCGCTGACCCATGTCGGTTATCCCCGCGATCTCGCTTTGATCGCCAAGATCCCGGACGTCGACGTC
GTCGTCGGCGGCCACTCCCACAGCCTGCTCTCCAATACCGACCCCAAGGCCGAAGGCCCCTATCCGACGATGGTCGACAA
TCCGGGCGGCTACAAGGTGCCGGTCGTCCAGGCCGCCTCCTACAGCAAGTATCTCGGCGATCTCGTCGTCAATTTCGACG
ATAGCGGCGTCGTCAAGGATGCCAAGGGCGATCCGATCCTGATCGATTCCAGCTTTACGCCCGATCCGGCTGTCGTTGCC
CGCATCGCGGAACTGGCCAAACCGATCGAGGAACTGCGCAAGAAGGTCATCGGCTCCTCCGACGCCCCGATCGACGGCGA
CCGCAAGGTCTGCCGCGTCAAGGAATGCTCGATGGGCAATCTGGTGGCCGATGCCATGCTCGACCGCACGAAGAACCAGG
GTGTTGCCATCGCTTTCCAGAACGGCGGCGGCCTGCGCGCTTCGATCGATGGCGGTGAGGTCACCCAGGGCGAAGTCATC
ACCGTCCTGCCCTTCCAGAACACGCTCGCCACCTTCGAGGCCACCGGCGCAGATGTTGTCAAGGCGCTCGAAAACGGCGT
CAGCCAGATCGATCAGGGCGCCGGCCGCTTCCCGCAGGTCGCCGGCCTGAAATTCTCCTTCGACCAGTCCAAGCCTGTCG
GCAGCCGCGTCAGCGATGTCCAGGTGAAGGAGGGCGACACCTTCGCTCCGATCGACCAGGCCAAGACTTATAAGGTCGCC
ACCAACAACTTCATGCGGGCCGGCGGCGACGGTTATTCGATCTTCAAGGAAGGCAAGAACGCCTATGATTTCGGTCCGGA
TCTGGCCGACGTGACCGCCGAATATCTGGCCGCCCACTCGCCCTATAAGCCCTATACCGACGGCCGCATCACCGAAAACG
GCGCGGCAGTCGCGCAGGCACCGGCTTCCGAACCGGCCGCTCCCGCACCAGCAACGCCGGCAGCCCCTGCACCAACCACA
GAACCTGCCCCCGCTCCTGCTGCACCGGCAACGCCTGCGCCTGCGGCACCGGCAGCACCAGCGCCCGCAACACCAGCAAC
GCCCGCGCCCGCCGCGGAGCCGACACCGGCGCCGGCAGCCTCCGCACCTGCCGGAACGACGCCGTCCACCCACGTCATCG
CCGCCGGCGACACCTTCTGGGATCTCGCCGTGACCTTCTATGGCGACGGCACGCTGTGGCGGAAGCTTTCGGAGGCCAAC
GGCAGCCCGAACCCGCGTCACCTGACGGTCGGCAAGGAGATCGAGGTTCCCGCCAAGTAA

Upstream 100 bases:

>100_bases
TCGTCTGATGGAACATCCGCAGGCTGGGAACCGCCCCGGCGGATTAGGAAGGGATCGGCGAGGATCAGGGGGATATCTCG
CCTCAATCAGGAGAACAGGG

Downstream 100 bases:

>100_bases
GACGATTTGTCCCGATGGCACTGGCCGGCCCGGGCTTTCCCGGGCCGGCTTTTCTTTCTATAGGGTGAACCACGATCTCG
GGGCTGCGTATGAGCCGACG

Product: 5'-nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MTKSFSFGLLTASMLALSTGAAFADYELNILHINDFHSRIESINKFDSTCSAEEEGKKECFGGAARLKTAIDQRRQALSG
KNVLLLNAGDNFQGSLFYTTYKGAAEAEFLNLMKFDAMTVGNHEFDDSEDGLATFLDKVQFPVVTANVKAAAASKLGDRI
KPSLVLDVGGQKIGIVGAVTNDTAELSSPGPNVTISDDVQAITSAVQDLKGQGVNKIIALTHVGYPRDLALIAKIPDVDV
VVGGHSHSLLSNTDPKAEGPYPTMVDNPGGYKVPVVQAASYSKYLGDLVVNFDDSGVVKDAKGDPILIDSSFTPDPAVVA
RIAELAKPIEELRKKVIGSSDAPIDGDRKVCRVKECSMGNLVADAMLDRTKNQGVAIAFQNGGGLRASIDGGEVTQGEVI
TVLPFQNTLATFEATGADVVKALENGVSQIDQGAGRFPQVAGLKFSFDQSKPVGSRVSDVQVKEGDTFAPIDQAKTYKVA
TNNFMRAGGDGYSIFKEGKNAYDFGPDLADVTAEYLAAHSPYKPYTDGRITENGAAVAQAPASEPAAPAPATPAAPAPTT
EPAPAPAAPATPAPAAPAAPAPATPATPAPAAEPTPAPAASAPAGTTPSTHVIAAGDTFWDLAVTFYGDGTLWRKLSEAN
GSPNPRHLTVGKEIEVPAK

Sequences:

>Translated_659_residues
MTKSFSFGLLTASMLALSTGAAFADYELNILHINDFHSRIESINKFDSTCSAEEEGKKECFGGAARLKTAIDQRRQALSG
KNVLLLNAGDNFQGSLFYTTYKGAAEAEFLNLMKFDAMTVGNHEFDDSEDGLATFLDKVQFPVVTANVKAAAASKLGDRI
KPSLVLDVGGQKIGIVGAVTNDTAELSSPGPNVTISDDVQAITSAVQDLKGQGVNKIIALTHVGYPRDLALIAKIPDVDV
VVGGHSHSLLSNTDPKAEGPYPTMVDNPGGYKVPVVQAASYSKYLGDLVVNFDDSGVVKDAKGDPILIDSSFTPDPAVVA
RIAELAKPIEELRKKVIGSSDAPIDGDRKVCRVKECSMGNLVADAMLDRTKNQGVAIAFQNGGGLRASIDGGEVTQGEVI
TVLPFQNTLATFEATGADVVKALENGVSQIDQGAGRFPQVAGLKFSFDQSKPVGSRVSDVQVKEGDTFAPIDQAKTYKVA
TNNFMRAGGDGYSIFKEGKNAYDFGPDLADVTAEYLAAHSPYKPYTDGRITENGAAVAQAPASEPAAPAPATPAAPAPTT
EPAPAPAAPATPAPAAPAAPAPATPATPAPAAEPTPAPAASAPAGTTPSTHVIAAGDTFWDLAVTFYGDGTLWRKLSEAN
GSPNPRHLTVGKEIEVPAK
>Mature_658_residues
TKSFSFGLLTASMLALSTGAAFADYELNILHINDFHSRIESINKFDSTCSAEEEGKKECFGGAARLKTAIDQRRQALSGK
NVLLLNAGDNFQGSLFYTTYKGAAEAEFLNLMKFDAMTVGNHEFDDSEDGLATFLDKVQFPVVTANVKAAAASKLGDRIK
PSLVLDVGGQKIGIVGAVTNDTAELSSPGPNVTISDDVQAITSAVQDLKGQGVNKIIALTHVGYPRDLALIAKIPDVDVV
VGGHSHSLLSNTDPKAEGPYPTMVDNPGGYKVPVVQAASYSKYLGDLVVNFDDSGVVKDAKGDPILIDSSFTPDPAVVAR
IAELAKPIEELRKKVIGSSDAPIDGDRKVCRVKECSMGNLVADAMLDRTKNQGVAIAFQNGGGLRASIDGGEVTQGEVIT
VLPFQNTLATFEATGADVVKALENGVSQIDQGAGRFPQVAGLKFSFDQSKPVGSRVSDVQVKEGDTFAPIDQAKTYKVAT
NNFMRAGGDGYSIFKEGKNAYDFGPDLADVTAEYLAAHSPYKPYTDGRITENGAAVAQAPASEPAAPAPATPAAPAPTTE
PAPAPAAPATPAPAAPAAPAPATPATPAPAAEPTPAPAASAPAGTTPSTHVIAAGDTFWDLAVTFYGDGTLWRKLSEANG
SPNPRHLTVGKEIEVPAK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=541, Percent_Identity=37.7079482439926, Blast_Score=337, Evalue=3e-92,
Organism=Escherichia coli, GI1786687, Length=571, Percent_Identity=28.1961471103327, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1790658, Length=274, Percent_Identity=28.4671532846715, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28573524, Length=534, Percent_Identity=35.7677902621723, Blast_Score=337, Evalue=2e-92,
Organism=Drosophila melanogaster, GI19922446, Length=528, Percent_Identity=34.8484848484849, Blast_Score=323, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24654424, Length=528, Percent_Identity=34.8484848484849, Blast_Score=323, Evalue=3e-88,
Organism=Drosophila melanogaster, GI19922444, Length=545, Percent_Identity=35.045871559633, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24641187, Length=525, Percent_Identity=33.7142857142857, Blast_Score=311, Evalue=9e-85,
Organism=Drosophila melanogaster, GI221329836, Length=525, Percent_Identity=31.6190476190476, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI19921980, Length=498, Percent_Identity=28.9156626506024, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI161076508, Length=498, Percent_Identity=28.9156626506024, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24652512, Length=498, Percent_Identity=28.9156626506024, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 68597; Mature: 68466

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSFSFGLLTASMLALSTGAAFADYELNILHINDFHSRIESINKFDSTCSAEEEGKKEC
CCCCCCHHHHHHHHHHHHCCCEEECEEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHH
FGGAARLKTAIDQRRQALSGKNVLLLNAGDNFQGSLFYTTYKGAAEAEFLNLMKFDAMTV
HCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCEEEC
GNHEFDDSEDGLATFLDKVQFPVVTANVKAAAASKLGDRIKPSLVLDVGGQKIGIVGAVT
CCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCEEEEEECCCEEEEEEEEC
NDTAELSSPGPNVTISDDVQAITSAVQDLKGQGVNKIIALTHVGYPRDLALIAKIPDVDV
CCCHHCCCCCCCEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCEE
VVGGHSHSLLSNTDPKAEGPYPTMVDNPGGYKVPVVQAASYSKYLGDLVVNFDDSGVVKD
EECCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEECCHHHHHHCCEEEEECCCCCEEC
AKGDPILIDSSFTPDPAVVARIAELAKPIEELRKKVIGSSDAPIDGDRKVCRVKECSMGN
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCH
LVADAMLDRTKNQGVAIAFQNGGGLRASIDGGEVTQGEVITVLPFQNTLATFEATGADVV
HHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCCHHHEECCCHHHH
KALENGVSQIDQGAGRFPQVAGLKFSFDQSKPVGSRVSDVQVKEGDTFAPIDQAKTYKVA
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEE
TNNFMRAGGDGYSIFKEGKNAYDFGPDLADVTAEYLAAHSPYKPYTDGRITENGAAVAQA
CCCEEECCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEC
PASEPAAPAPATPAAPAPTTEPAPAPAAPATPAPAAPAAPAPATPATPAPAAEPTPAPAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SAPAGTTPSTHVIAAGDTFWDLAVTFYGDGTLWRKLSEANGSPNPRHLTVGKEIEVPAK
CCCCCCCCCCEEEECCCCEEEEEEEEECCCHHHHHHHHCCCCCCCCEEECCCEECCCCC
>Mature Secondary Structure 
TKSFSFGLLTASMLALSTGAAFADYELNILHINDFHSRIESINKFDSTCSAEEEGKKEC
CCCCCHHHHHHHHHHHHCCCEEECEEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHH
FGGAARLKTAIDQRRQALSGKNVLLLNAGDNFQGSLFYTTYKGAAEAEFLNLMKFDAMTV
HCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCEEEC
GNHEFDDSEDGLATFLDKVQFPVVTANVKAAAASKLGDRIKPSLVLDVGGQKIGIVGAVT
CCCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCEEEEEECCCEEEEEEEEC
NDTAELSSPGPNVTISDDVQAITSAVQDLKGQGVNKIIALTHVGYPRDLALIAKIPDVDV
CCCHHCCCCCCCEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCEE
VVGGHSHSLLSNTDPKAEGPYPTMVDNPGGYKVPVVQAASYSKYLGDLVVNFDDSGVVKD
EECCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEECCHHHHHHCCEEEEECCCCCEEC
AKGDPILIDSSFTPDPAVVARIAELAKPIEELRKKVIGSSDAPIDGDRKVCRVKECSMGN
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCH
LVADAMLDRTKNQGVAIAFQNGGGLRASIDGGEVTQGEVITVLPFQNTLATFEATGADVV
HHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCCHHHEECCCHHHH
KALENGVSQIDQGAGRFPQVAGLKFSFDQSKPVGSRVSDVQVKEGDTFAPIDQAKTYKVA
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEE
TNNFMRAGGDGYSIFKEGKNAYDFGPDLADVTAEYLAAHSPYKPYTDGRITENGAAVAQA
CCCEEECCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEC
PASEPAAPAPATPAAPAPTTEPAPAPAAPATPAPAAPAAPAPATPATPAPAAEPTPAPAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SAPAGTTPSTHVIAAGDTFWDLAVTFYGDGTLWRKLSEANGSPNPRHLTVGKEIEVPAK
CCCCCCCCCCEEEECCCCEEEEEEEEECCCHHHHHHHHCCCCCCCCEEECCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]