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The map label for this gene is thiE [H]

Identifier: 209550845

GI number: 209550845

Start: 3361087

End: 3361740

Strand: Reverse

Name: thiE [H]

Synonym: Rleg2_3269

Alternate gene names: 209550845

Gene position: 3361740-3361087 (Counterclockwise)

Preceding gene: 209550848

Following gene: 209550844

Centisome position: 74.08

GC content: 63.3

Gene sequence:

>654_bases
ATGACCGAACCGGAAAACCGCTGCCGCCTTGTGCTCATCGTCCCCGATATTGCCGATGCCGATGAACAGGCAAGGATCGT
TGCCGACGCGCTGAAGGGCGGCGATGTCGCTTCGGTGATCGTGCCGCAATACGGGCTCGACGACGGTACCTTCCAGAAAC
ATGCGGAGAAGCTGGTGCCGCTGATCCAGGATGCCGGGGCGGCCGCCCTGATTGCAGGCGACAGCCGCGTGGCAGGACGG
GCAAAGGCCGACGGCCTGCACCTTTCCGGCAATGCCGAGGCACTTTCGGAAGCGATCGAAAAACATGCGCCGAAGCTGAT
CGTCGGCGGCGGCAACGCAGCCGACCGGCACAATGCGCTGGAGATCGGCGAGGCCAGGCCGGATTATATTTTCTTCGGCA
AGCTCGACGGCGATATCAAGCCGGAGGTCCATCCGAAGAACCTTGCGCTCGGCGAATGGTGGGCCTCGATGATCGAGATT
CCCTGCATCGTCATGGGCGGCACCGATCCGGCCTCGGCGCTTGCCGTCGCCGAGACCGGAGCGGAATTCGTGGCGCTGCG
GCTGGCTGTCTTCGGCGAGCCCAACCGGGCGCCGTCTATCGTCGCCGAAGTCAACGCTTTGCTTGACGAAAAAGCGCCAC
GGTTTGAGGATTGA

Upstream 100 bases:

>100_bases
CGTCCTCCTCTTGCAAGCCTCGTCTAACGGAACTACTCACACAAAACGAGTGCGGATCCCTCCTCTCAACGGGGAATTCG
CGAGGAAACGGAAAGACCTG

Downstream 100 bases:

>100_bases
AATCGCGCTCATGCCGATCCCCACCGCCCGCCCGTTGAAATTTATCCTTGCCAGCATGGCCGCCTTCGTTGCGGCCGGAC
CGGCTGTCGTCTTGGCGCAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTEPENRCRLVLIVPDIADADEQARIVADALKGGDVASVIVPQYGLDDGTFQKHAEKLVPLIQDAGAAALIAGDSRVAGR
AKADGLHLSGNAEALSEAIEKHAPKLIVGGGNAADRHNALEIGEARPDYIFFGKLDGDIKPEVHPKNLALGEWWASMIEI
PCIVMGGTDPASALAVAETGAEFVALRLAVFGEPNRAPSIVAEVNALLDEKAPRFED

Sequences:

>Translated_217_residues
MTEPENRCRLVLIVPDIADADEQARIVADALKGGDVASVIVPQYGLDDGTFQKHAEKLVPLIQDAGAAALIAGDSRVAGR
AKADGLHLSGNAEALSEAIEKHAPKLIVGGGNAADRHNALEIGEARPDYIFFGKLDGDIKPEVHPKNLALGEWWASMIEI
PCIVMGGTDPASALAVAETGAEFVALRLAVFGEPNRAPSIVAEVNALLDEKAPRFED
>Mature_216_residues
TEPENRCRLVLIVPDIADADEQARIVADALKGGDVASVIVPQYGLDDGTFQKHAEKLVPLIQDAGAAALIAGDSRVAGRA
KADGLHLSGNAEALSEAIEKHAPKLIVGGGNAADRHNALEIGEARPDYIFFGKLDGDIKPEVHPKNLALGEWWASMIEIP
CIVMGGTDPASALAVAETGAEFVALRLAVFGEPNRAPSIVAEVNALLDEKAPRFED

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22844; Mature: 22713

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPENRCRLVLIVPDIADADEQARIVADALKGGDVASVIVPQYGLDDGTFQKHAEKLVP
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
LIQDAGAAALIAGDSRVAGRAKADGLHLSGNAEALSEAIEKHAPKLIVGGGNAADRHNAL
HHHCCCCEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCEEEEECCCCCCCCCCE
EIGEARPDYIFFGKLDGDIKPEVHPKNLALGEWWASMIEIPCIVMGGTDPASALAVAETG
EECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHHEEHHHCC
AEFVALRLAVFGEPNRAPSIVAEVNALLDEKAPRFED
HHHEEEEHEEECCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEPENRCRLVLIVPDIADADEQARIVADALKGGDVASVIVPQYGLDDGTFQKHAEKLVP
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
LIQDAGAAALIAGDSRVAGRAKADGLHLSGNAEALSEAIEKHAPKLIVGGGNAADRHNAL
HHHCCCCEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCEEEEECCCCCCCCCCE
EIGEARPDYIFFGKLDGDIKPEVHPKNLALGEWWASMIEIPCIVMGGTDPASALAVAETG
EECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHHEEHHHCC
AEFVALRLAVFGEPNRAPSIVAEVNALLDEKAPRFED
HHHEEEEHEEECCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA