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The map label for this gene is pgk [H]

Identifier: 209550817

GI number: 209550817

Start: 3330562

End: 3331764

Strand: Direct

Name: pgk [H]

Synonym: Rleg2_3241

Alternate gene names: 209550817

Gene position: 3330562-3331764 (Clockwise)

Preceding gene: 209550816

Following gene: 209550818

Centisome position: 73.39

GC content: 64.42

Gene sequence:

>1203_bases
ATGCCTTCCTTCAAGACCCTCGACGATCTTTCCGACATCCGCGGCAAGCGCGTTCTCGTCCGCGTCGATCTCAACGTCCC
GGTCAAGGACGGCAAGGTCACCGATACGACCCGCATCGAGCGTGTGGCGCCGACGATCCTCGAATTGTCCGAAAAGGGCG
CCAAGGTCATCCTGCTGGCCCATTTCGGCCGGCCGAAGGACGGCCCTTCGCCTGAACTGTCGCTGTCGCTGATCGCCCCT
TCCGTCGAGGAAGTACTGGATCATGCCGTGTCGACCGCCTCCGACTGCATCGGCGAGGCCGCCGCCTCCGCCGTCGCCGC
GATGAATGACGGCGATATCCTGCTTCTGGAAAACACCCGCTTCCACAAGGGCGAGGAAAAGAACGATCCCGACTTCACCA
AGGCGCTTGCCGCCAACGGCGACATCTATGTCAATGACGCCTTCTCCGCCGCCCACCGCGCCCATGCCTCGACCGAGGGG
CTTGCCCACCATCTGCCGGCCTATGCCGGCCGCACCATGCAGGCCGAGCTGGAAGCACTGGAAAAGGGTCTGGGTGATCC
CGCCCGCCCGGTCGTCGCAATTGTCGGCGGCGCCAAGGTCTCGACCAAGATCGACCTGCTGATGAACCTGGTGAAAAAAG
TCGATGCGCTGGTCATCGGCGGCGGCATGGCCAATACCTTCATCGCCGCCCGCGGCACCAATGTCGGCAAATCGCTCTGC
GAGCATGATCTTGCCGAAACCGCCAGGCAGATCATGATCGAGGCCGCGACATCGGGCTGCGCCATCATCCTGCCGGAAGA
CGGCGTGATTGCCCGCGAGTTCAAGGCGGGTGCCGCCAACGAGACGGTCGATATCGACGCCATTCCCGCCGATGCCATGG
TGCTCGATGTCGGCCCGAAATCCGTCCAGGCCATCAGCGCCTGGATCGAGCGCGCTTCGACCCTCGTCTGGAACGGCCCG
CTCGGCGCCTTCGAGATCGAGCCCTTCGATGCCGCAACCGTCGGCGCTGCGAAATACGCAGCCGAGCGCACCACAGCCGG
CAAGCTCACCTCCGTTGCCGGCGGCGGCGACACGGTTTCTGCGCTGAACCACGCCGGTGTTGCCGACGATTTCACCTATG
TCTCCACGGCCGGCGGCGCTTTCCTCGAATGGATGGAAGGCAAGGAGCTTCCCGGCGTCGCCGTCCTCAACGCCGCTCGG
TAA

Upstream 100 bases:

>100_bases
CGACGAAGGCACACACGAAAACGGTCGCTGAACGCCTTGCGTCTCGAATGATCCTTGCTATGGTCGGCGCAATTTCCGCC
AACCAAGACTGGATCTCCCC

Downstream 100 bases:

>100_bases
CTGCCTCATGCATCGGCCTGACAATCGGGCCGATGCATCCGTTTAAAATGTTGGCAGCCTCCTTTGCGCAGGCAAAAGGC
GCGCGGCGCGGCAAGCCCGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MPSFKTLDDLSDIRGKRVLVRVDLNVPVKDGKVTDTTRIERVAPTILELSEKGAKVILLAHFGRPKDGPSPELSLSLIAP
SVEEVLDHAVSTASDCIGEAAASAVAAMNDGDILLLENTRFHKGEEKNDPDFTKALAANGDIYVNDAFSAAHRAHASTEG
LAHHLPAYAGRTMQAELEALEKGLGDPARPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFIAARGTNVGKSLC
EHDLAETARQIMIEAATSGCAIILPEDGVIAREFKAGAANETVDIDAIPADAMVLDVGPKSVQAISAWIERASTLVWNGP
LGAFEIEPFDAATVGAAKYAAERTTAGKLTSVAGGGDTVSALNHAGVADDFTYVSTAGGAFLEWMEGKELPGVAVLNAAR

Sequences:

>Translated_400_residues
MPSFKTLDDLSDIRGKRVLVRVDLNVPVKDGKVTDTTRIERVAPTILELSEKGAKVILLAHFGRPKDGPSPELSLSLIAP
SVEEVLDHAVSTASDCIGEAAASAVAAMNDGDILLLENTRFHKGEEKNDPDFTKALAANGDIYVNDAFSAAHRAHASTEG
LAHHLPAYAGRTMQAELEALEKGLGDPARPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFIAARGTNVGKSLC
EHDLAETARQIMIEAATSGCAIILPEDGVIAREFKAGAANETVDIDAIPADAMVLDVGPKSVQAISAWIERASTLVWNGP
LGAFEIEPFDAATVGAAKYAAERTTAGKLTSVAGGGDTVSALNHAGVADDFTYVSTAGGAFLEWMEGKELPGVAVLNAAR
>Mature_399_residues
PSFKTLDDLSDIRGKRVLVRVDLNVPVKDGKVTDTTRIERVAPTILELSEKGAKVILLAHFGRPKDGPSPELSLSLIAPS
VEEVLDHAVSTASDCIGEAAASAVAAMNDGDILLLENTRFHKGEEKNDPDFTKALAANGDIYVNDAFSAAHRAHASTEGL
AHHLPAYAGRTMQAELEALEKGLGDPARPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFIAARGTNVGKSLCE
HDLAETARQIMIEAATSGCAIILPEDGVIAREFKAGAANETVDIDAIPADAMVLDVGPKSVQAISAWIERASTLVWNGPL
GAFEIEPFDAATVGAAKYAAERTTAGKLTSVAGGGDTVSALNHAGVADDFTYVSTAGGAFLEWMEGKELPGVAVLNAAR

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=40.0485436893204, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=40.0485436893204, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1789294, Length=387, Percent_Identity=44.1860465116279, Blast_Score=302, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17508823, Length=410, Percent_Identity=41.7073170731707, Blast_Score=289, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=40.4358353510896, Blast_Score=271, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24581203, Length=407, Percent_Identity=41.5233415233415, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI17136394, Length=406, Percent_Identity=39.4088669950739, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41652; Mature: 41520

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFKTLDDLSDIRGKRVLVRVDLNVPVKDGKVTDTTRIERVAPTILELSEKGAKVILLA
CCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
HFGRPKDGPSPELSLSLIAPSVEEVLDHAVSTASDCIGEAAASAVAAMNDGDILLLENTR
ECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCC
FHKGEEKNDPDFTKALAANGDIYVNDAFSAAHRAHASTEGLAHHLPAYAGRTMQAELEAL
CCCCCCCCCCHHHHHHHCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
EKGLGDPARPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFIAARGTNVGKSLC
HHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCHHHEEEECCCHHHHHHH
EHDLAETARQIMIEAATSGCAIILPEDGVIAREFKAGAANETVDIDAIPADAMVLDVGPK
HHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHCCCCCCCEEEEECCCCCEEEEECCCH
SVQAISAWIERASTLVWNGPLGAFEIEPFDAATVGAAKYAAERTTAGKLTSVAGGGDTVS
HHHHHHHHHHHHCEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHH
ALNHAGVADDFTYVSTAGGAFLEWMEGKELPGVAVLNAAR
HHHCCCCCCCCCEEECCCCHHHHHHCCCCCCCEEEEECCC
>Mature Secondary Structure 
PSFKTLDDLSDIRGKRVLVRVDLNVPVKDGKVTDTTRIERVAPTILELSEKGAKVILLA
CCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
HFGRPKDGPSPELSLSLIAPSVEEVLDHAVSTASDCIGEAAASAVAAMNDGDILLLENTR
ECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCC
FHKGEEKNDPDFTKALAANGDIYVNDAFSAAHRAHASTEGLAHHLPAYAGRTMQAELEAL
CCCCCCCCCCHHHHHHHCCCCEEEECHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHH
EKGLGDPARPVVAIVGGAKVSTKIDLLMNLVKKVDALVIGGGMANTFIAARGTNVGKSLC
HHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCHHHEEEECCCHHHHHHH
EHDLAETARQIMIEAATSGCAIILPEDGVIAREFKAGAANETVDIDAIPADAMVLDVGPK
HHHHHHHHHHHHHHCCCCCCEEEECCCCEEEHHHCCCCCCCEEEEECCCCCEEEEECCCH
SVQAISAWIERASTLVWNGPLGAFEIEPFDAATVGAAKYAAERTTAGKLTSVAGGGDTVS
HHHHHHHHHHHHCEEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHH
ALNHAGVADDFTYVSTAGGAFLEWMEGKELPGVAVLNAAR
HHHCCCCCCCCCEEECCCCHHHHHHCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA