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The map label for this gene is tilS [H]

Identifier: 209550773

GI number: 209550773

Start: 3286183

End: 3287661

Strand: Reverse

Name: tilS [H]

Synonym: Rleg2_3197

Alternate gene names: 209550773

Gene position: 3287661-3286183 (Counterclockwise)

Preceding gene: 209550774

Following gene: 209550772

Centisome position: 72.45

GC content: 62.47

Gene sequence:

>1479_bases
ATGTCTCCGGAAGCCCGATCGCCGCGAGAGGCGATCCTCCAGTTTCTCAGCTCGCTTCAAAGCCCTGTACGCATTCTCGT
CGCCATATCGGGCGGCAGCGATTCCACCGGCCTGCTTCTCCTTCTCGATGAAGCCGTCAAGGCCGCGCCGCATCTCAAAA
TTTCCCTATGCGCTGCGACCGTCGATCATGCGTTGCGCGCCGGTTCCGCAGACGAGGCGCGAGAAGTTGCAGCCCTCTGC
GCGTCGCTCGGTATTGCCCATGCCATCATGACCTGGCAGGGCGACAAGCCGATAACCGGCATCATGGCCGCGGCCCGCGA
GGCGCGTTACAGCTTGCTCGCCGAAGCGGCGGAAGCTCTTGGCGCCGATCTCATCGTCACCGCACATACGCTCGACGATC
AGCGTGAAACGCTTGCCATGCGCGGCATGCGAACGGAGGAGGTTTCGACCGGTATTGCCGATGCGGTGCTCTTCGACCGG
CATTTCTGGATCTTGCGGCCGCTTCTCTTTTCCAGCCGGGCCGATATCCGCGCTTTCCTGAGCGAGCAGGGCGCGGGGTG
GATCGACGATCCGAGCAACGAAGATGTGAAATACGAGCGCGTGCGAATCCGGCAGCAGCTTTCGGCCGATGGAGGTGCCG
AGGCGAATATCAGTGCCGTCTGGGACGAGCGACTGGCTGTTTCGGCCAAGGGAGCGGAATGGCTGGATCACCATTTCAAG
CTTCACGGCGGTCTGCTCGGGCAAGTGATGCCTGAAGGGTTGCGGCAGGATCGCGCCGTTCTCGACTATGTGCTTGGTCG
TCTCACGGCCGTTTTCGGCGGACAGCCGTTTGCGCCGGGGAGGGCGCAAATGGAGCGTCTCGTTGCATTTGCCGCCGGCG
GAGAGCCGGCGCGGATGACGGTCGGCCGGGTGGTCTTCGATCTCAGGCGGGACGGGCTTTATCTGACGCGCGAAAGCCGG
GGGATCTTGCCGCTGGTCTTGCAGCCCGGGGAGGCGGGCGTGTGGGATGGGAGGTTTGAGGCGGTCAATAGGTCGGCGAT
TGATGTGAGGATAGAAGCGCAATCCGGCCGTTCCTCCCTCATTCCTGTGCTCGTCACAGGAATCCAGCCACCGCGCGTCG
GCGCGGTGAATGACTCGACAATACCCAAGGAGTCTCCCGCGCCCAAGGACTTGGGCGCGCTGGATTCCTGTGACATCCCT
CGGGTCAAGCCCGAGGACAGGAATGAGGGAGGGTGGGGCGCTGGCCTGCCCAAGGCGGCATGGAAGCGCGCCATCGCCTC
AGCGCCGGCCATATCGGCGCAAGAGGTCCCTTTATCTGCGGAAACCGCCGAGGCGATCCACCTCACGCCCTATTTCGCAC
CCTTCGACCGCTTTTTGACACGATTTGATTTCATCTTTGCCAACAGGCTTTCGGCGGTTTTCGCGACGGCGCCCTATGCG
AGGCCGCCTTTAAGAAGTATTGACGGAAAAACCATCTGA

Upstream 100 bases:

>100_bases
CGCTGCGCGAAGTCTCGAAGCGGTATCCGAAGGCTTCGCGTGCCGTCATAAGCAAGGTTGCGAGCGAACAGAAGCGCCTC
GCCTGCTAAGGTCTTCCGGC

Downstream 100 bases:

>100_bases
CCTGGGGTGTCGCCTTGGCAATCGCACGGCGCAACCCTATGTTAGAAACGAATAAGACGGTCGCCATGAGGCGGCTGTTC
CAGTGCTGGGGAGTTCAATG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSPEARSPREAILQFLSSLQSPVRILVAISGGSDSTGLLLLLDEAVKAAPHLKISLCAATVDHALRAGSADEAREVAALC
ASLGIAHAIMTWQGDKPITGIMAAAREARYSLLAEAAEALGADLIVTAHTLDDQRETLAMRGMRTEEVSTGIADAVLFDR
HFWILRPLLFSSRADIRAFLSEQGAGWIDDPSNEDVKYERVRIRQQLSADGGAEANISAVWDERLAVSAKGAEWLDHHFK
LHGGLLGQVMPEGLRQDRAVLDYVLGRLTAVFGGQPFAPGRAQMERLVAFAAGGEPARMTVGRVVFDLRRDGLYLTRESR
GILPLVLQPGEAGVWDGRFEAVNRSAIDVRIEAQSGRSSLIPVLVTGIQPPRVGAVNDSTIPKESPAPKDLGALDSCDIP
RVKPEDRNEGGWGAGLPKAAWKRAIASAPAISAQEVPLSAETAEAIHLTPYFAPFDRFLTRFDFIFANRLSAVFATAPYA
RPPLRSIDGKTI

Sequences:

>Translated_492_residues
MSPEARSPREAILQFLSSLQSPVRILVAISGGSDSTGLLLLLDEAVKAAPHLKISLCAATVDHALRAGSADEAREVAALC
ASLGIAHAIMTWQGDKPITGIMAAAREARYSLLAEAAEALGADLIVTAHTLDDQRETLAMRGMRTEEVSTGIADAVLFDR
HFWILRPLLFSSRADIRAFLSEQGAGWIDDPSNEDVKYERVRIRQQLSADGGAEANISAVWDERLAVSAKGAEWLDHHFK
LHGGLLGQVMPEGLRQDRAVLDYVLGRLTAVFGGQPFAPGRAQMERLVAFAAGGEPARMTVGRVVFDLRRDGLYLTRESR
GILPLVLQPGEAGVWDGRFEAVNRSAIDVRIEAQSGRSSLIPVLVTGIQPPRVGAVNDSTIPKESPAPKDLGALDSCDIP
RVKPEDRNEGGWGAGLPKAAWKRAIASAPAISAQEVPLSAETAEAIHLTPYFAPFDRFLTRFDFIFANRLSAVFATAPYA
RPPLRSIDGKTI
>Mature_491_residues
SPEARSPREAILQFLSSLQSPVRILVAISGGSDSTGLLLLLDEAVKAAPHLKISLCAATVDHALRAGSADEAREVAALCA
SLGIAHAIMTWQGDKPITGIMAAAREARYSLLAEAAEALGADLIVTAHTLDDQRETLAMRGMRTEEVSTGIADAVLFDRH
FWILRPLLFSSRADIRAFLSEQGAGWIDDPSNEDVKYERVRIRQQLSADGGAEANISAVWDERLAVSAKGAEWLDHHFKL
HGGLLGQVMPEGLRQDRAVLDYVLGRLTAVFGGQPFAPGRAQMERLVAFAAGGEPARMTVGRVVFDLRRDGLYLTRESRG
ILPLVLQPGEAGVWDGRFEAVNRSAIDVRIEAQSGRSSLIPVLVTGIQPPRVGAVNDSTIPKESPAPKDLGALDSCDIPR
VKPEDRNEGGWGAGLPKAAWKRAIASAPAISAQEVPLSAETAEAIHLTPYFAPFDRFLTRFDFIFANRLSAVFATAPYAR
PPLRSIDGKTI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=193, Percent_Identity=32.6424870466321, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 53012; Mature: 52881

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPEARSPREAILQFLSSLQSPVRILVAISGGSDSTGLLLLLDEAVKAAPHLKISLCAAT
CCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEHHHHCCCCCEEEHHHHH
VDHALRAGSADEAREVAALCASLGIAHAIMTWQGDKPITGIMAAAREARYSLLAEAAEAL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
GADLIVTAHTLDDQRETLAMRGMRTEEVSTGIADAVLFDRHFWILRPLLFSSRADIRAFL
CCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SEQGAGWIDDPSNEDVKYERVRIRQQLSADGGAEANISAVWDERLAVSAKGAEWLDHHFK
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHEEHHHHEEECCCHHHHHHHHH
LHGGLLGQVMPEGLRQDRAVLDYVLGRLTAVFGGQPFAPGRAQMERLVAFAAGGEPARMT
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHH
VGRVVFDLRRDGLYLTRESRGILPLVLQPGEAGVWDGRFEAVNRSAIDVRIEAQSGRSSL
HHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCC
IPVLVTGIQPPRVGAVNDSTIPKESPAPKDLGALDSCDIPRVKPEDRNEGGWGAGLPKAA
CHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
WKRAIASAPAISAQEVPLSAETAEAIHLTPYFAPFDRFLTRFDFIFANRLSAVFATAPYA
HHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
RPPLRSIDGKTI
CCCCCCCCCCCC
>Mature Secondary Structure 
SPEARSPREAILQFLSSLQSPVRILVAISGGSDSTGLLLLLDEAVKAAPHLKISLCAAT
CCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEHHHHCCCCCEEEHHHHH
VDHALRAGSADEAREVAALCASLGIAHAIMTWQGDKPITGIMAAAREARYSLLAEAAEAL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
GADLIVTAHTLDDQRETLAMRGMRTEEVSTGIADAVLFDRHFWILRPLLFSSRADIRAFL
CCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SEQGAGWIDDPSNEDVKYERVRIRQQLSADGGAEANISAVWDERLAVSAKGAEWLDHHFK
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHEEHHHHEEECCCHHHHHHHHH
LHGGLLGQVMPEGLRQDRAVLDYVLGRLTAVFGGQPFAPGRAQMERLVAFAAGGEPARMT
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHH
VGRVVFDLRRDGLYLTRESRGILPLVLQPGEAGVWDGRFEAVNRSAIDVRIEAQSGRSSL
HHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCC
IPVLVTGIQPPRVGAVNDSTIPKESPAPKDLGALDSCDIPRVKPEDRNEGGWGAGLPKAA
CHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
WKRAIASAPAISAQEVPLSAETAEAIHLTPYFAPFDRFLTRFDFIFANRLSAVFATAPYA
HHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
RPPLRSIDGKTI
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]