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The map label for this gene is braD [H]

Identifier: 209550638

GI number: 209550638

Start: 3120070

End: 3121722

Strand: Reverse

Name: braD [H]

Synonym: Rleg2_3062

Alternate gene names: 209550638

Gene position: 3121722-3120070 (Counterclockwise)

Preceding gene: 209550639

Following gene: 209550637

Centisome position: 68.79

GC content: 62.73

Gene sequence:

>1653_bases
ATGTATCGCGCCATAAAGATTTTCCTCATCACTGTCTGCCTGACATTCTCGGGCCCGACACTTTCCGGCCTCGCATTTCC
GGGCCTGGCAGCATCGAGCGAAGTTCGGGCCGAGGACGATATCCACGTGCTCATCGATGCGCTCGGCATCGGCGACTTCC
CGGAGCGCGAAGCGGCCATCAAGACGCTGGTCGCCTCCAAGGACCCGCATGTCAACCAGATCCTGCAGCAGTTGAGCGAC
GGCCTGCTCTACGTCAATTCCGACGGCGGCCCGGTGCTCCTCCAGGGCGGCACCGATGACGAACCGACCTATTCCGATCC
AATCACCGGCGAAGCCGCCGCCGATGTCGACCCTGACATGATGACCAAGGTCAAGATCAACAACGCACTTCGCGGTGTGA
TCAGCGCCGCCACCAGCCAGCTGACGCTGATGAGCCCAGACCGCTCGGCGCGGCTCGCCGCGGCACAGGGTCTGCTGAAA
GACGCCGATCCCGCCAACCTCGACCTGCTGAACGCGGCGCTTGCGGCCGAAAAGGACGCCGAGATCAAGAATACGATGGA
AGCTGCGCGTGCGGTGCTGCTGCTCAAGACGGATGCGAGCGTCGAAGACAAGAAGGCTGCGATCGATACGATCGCGGCTC
GCGGCAATCGCGATGCGCTGACCATCCTCACGACCACGCTCGAAACCGCGCCTGACGATCTGAAGCCTGCGATCCAGGCC
GATATCAGCACCATCAATCGCAGCTTGGCGCTTTGGGATGTCGCCCAGAACGTCTGGTACGGCTTGTCGCTCGGCTCGGT
GCTGCTGCTTGCCGCGATCGGCCTTGCCATCACCTTCGGCGTTATGGGCGTCATCAACATGGCGCATGGCGAAATGGTGA
TGATCGGGGCCTATACGACCTATGTCGTGCAGGAATACATCACCTCGGCCTTCCCCGGCGTGGCGGACTATTCGCTTGCC
TTTGCCGTGCCTGCCGCCTTCGTCTTTACCGGTTTCGTCGGCCTGGTGATTGAGCGCGCCGTCATTCGCTATCTCTACGG
CCGCCCGCTGGAAACGCTGCTCGCCACCTGGGGCGTGTCGCTGATCCTGCAGCAGGCCGTGCGCAGCATCTTCGGCCCGA
CCAACCGCGAGGTCCGCAATCCAAGCTGGATGTCCGGCGTCTTCGATCTCGGCGGGCTCTCGATCACCTGGAACCGACTG
TGGATCATCGTCTTTTCGATGGTCGTCTTCGTGATGCTGCTCCTGCTGCTCAAGCGGTCGGCCTTCGGCCTGCAGATGCG
CGCCGTCACGCAGAACCGCCGCATGGCGTCCTCGATGGGCATCCGCACCGGCTGGGTCGATGCCTTCACCTTCGCGCTCG
GTTCGGGCATTGCCGGTATTGCCGGCGTGGCGCTCAGCCAGATCGACAACGTCTCGCCGAATCTCGGCCAATCCTACATC
ATCGACAGCTTCATGGTCGTCGTCTTCGGCGGTGTCGGCAATCTCTGGGGCACGCTGGTCGGCGCCCTTTCGCTCGGCGT
CGTCAACAAGTTCCTCGAGCCCTTCGCCGGCGCCGTGCTCGGCAAGATCCTGGTGCTCGTCCTCATCATTCTCTTCATCC
AGAAGCGTCCGCGCGGGCTCTTCGCACTCAAAGGAAGGGCGGTGGAAGCATGA

Upstream 100 bases:

>100_bases
ATGATTGCCTGAAGACTTCCGTCCGGGTTTGACACCCGGGCGGAAGCTCCGTCCCAGCGATTGCGCCGCGAGGCCAACAC
ACTCAAGGGCGAGAAAGCCG

Downstream 100 bases:

>100_bases
TAACGGCCTTCCTTCTCCGGTCACTCGATCGCAAGATCGTCATTGCCATCGCGCTGCTGCTACTGGTCGCCGTTCTCGTG
CCGGTCCTGAACCTGATGAC

Product: urea ABC transporter permease UrtB

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MYRAIKIFLITVCLTFSGPTLSGLAFPGLAASSEVRAEDDIHVLIDALGIGDFPEREAAIKTLVASKDPHVNQILQQLSD
GLLYVNSDGGPVLLQGGTDDEPTYSDPITGEAAADVDPDMMTKVKINNALRGVISAATSQLTLMSPDRSARLAAAQGLLK
DADPANLDLLNAALAAEKDAEIKNTMEAARAVLLLKTDASVEDKKAAIDTIAARGNRDALTILTTTLETAPDDLKPAIQA
DISTINRSLALWDVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMIGAYTTYVVQEYITSAFPGVADYSLA
FAVPAAFVFTGFVGLVIERAVIRYLYGRPLETLLATWGVSLILQQAVRSIFGPTNREVRNPSWMSGVFDLGGLSITWNRL
WIIVFSMVVFVMLLLLLKRSAFGLQMRAVTQNRRMASSMGIRTGWVDAFTFALGSGIAGIAGVALSQIDNVSPNLGQSYI
IDSFMVVVFGGVGNLWGTLVGALSLGVVNKFLEPFAGAVLGKILVLVLIILFIQKRPRGLFALKGRAVEA

Sequences:

>Translated_550_residues
MYRAIKIFLITVCLTFSGPTLSGLAFPGLAASSEVRAEDDIHVLIDALGIGDFPEREAAIKTLVASKDPHVNQILQQLSD
GLLYVNSDGGPVLLQGGTDDEPTYSDPITGEAAADVDPDMMTKVKINNALRGVISAATSQLTLMSPDRSARLAAAQGLLK
DADPANLDLLNAALAAEKDAEIKNTMEAARAVLLLKTDASVEDKKAAIDTIAARGNRDALTILTTTLETAPDDLKPAIQA
DISTINRSLALWDVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMIGAYTTYVVQEYITSAFPGVADYSLA
FAVPAAFVFTGFVGLVIERAVIRYLYGRPLETLLATWGVSLILQQAVRSIFGPTNREVRNPSWMSGVFDLGGLSITWNRL
WIIVFSMVVFVMLLLLLKRSAFGLQMRAVTQNRRMASSMGIRTGWVDAFTFALGSGIAGIAGVALSQIDNVSPNLGQSYI
IDSFMVVVFGGVGNLWGTLVGALSLGVVNKFLEPFAGAVLGKILVLVLIILFIQKRPRGLFALKGRAVEA
>Mature_550_residues
MYRAIKIFLITVCLTFSGPTLSGLAFPGLAASSEVRAEDDIHVLIDALGIGDFPEREAAIKTLVASKDPHVNQILQQLSD
GLLYVNSDGGPVLLQGGTDDEPTYSDPITGEAAADVDPDMMTKVKINNALRGVISAATSQLTLMSPDRSARLAAAQGLLK
DADPANLDLLNAALAAEKDAEIKNTMEAARAVLLLKTDASVEDKKAAIDTIAARGNRDALTILTTTLETAPDDLKPAIQA
DISTINRSLALWDVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMIGAYTTYVVQEYITSAFPGVADYSLA
FAVPAAFVFTGFVGLVIERAVIRYLYGRPLETLLATWGVSLILQQAVRSIFGPTNREVRNPSWMSGVFDLGGLSITWNRL
WIIVFSMVVFVMLLLLLKRSAFGLQMRAVTQNRRMASSMGIRTGWVDAFTFALGSGIAGIAGVALSQIDNVSPNLGQSYI
IDSFMVVVFGGVGNLWGTLVGALSLGVVNKFLEPFAGAVLGKILVLVLIILFIQKRPRGLFALKGRAVEA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=294, Percent_Identity=30.2721088435374, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 58624; Mature: 58624

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRAIKIFLITVCLTFSGPTLSGLAFPGLAASSEVRAEDDIHVLIDALGIGDFPEREAAI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHCCCCCCCHHHHH
KTLVASKDPHVNQILQQLSDGLLYVNSDGGPVLLQGGTDDEPTYSDPITGEAAADVDPDM
HHHHHCCCCCHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHH
MTKVKINNALRGVISAATSQLTLMSPDRSARLAAAQGLLKDADPANLDLLNAALAAEKDA
HHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCH
EIKNTMEAARAVLLLKTDASVEDKKAAIDTIAARGNRDALTILTTTLETAPDDLKPAIQA
HHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCHHHHHHHH
DISTINRSLALWDVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMIGAYTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHH
YVVQEYITSAFPGVADYSLAFAVPAAFVFTGFVGLVIERAVIRYLYGRPLETLLATWGVS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LILQQAVRSIFGPTNREVRNPSWMSGVFDLGGLSITWNRLWIIVFSMVVFVMLLLLLKRS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCH
AFGLQMRAVTQNRRMASSMGIRTGWVDAFTFALGSGIAGIAGVALSQIDNVSPNLGQSYI
HCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
IDSFMVVVFGGVGNLWGTLVGALSLGVVNKFLEPFAGAVLGKILVLVLIILFIQKRPRGL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FALKGRAVEA
EEECCCCCCC
>Mature Secondary Structure
MYRAIKIFLITVCLTFSGPTLSGLAFPGLAASSEVRAEDDIHVLIDALGIGDFPEREAAI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEHHCCCCCCCHHHHH
KTLVASKDPHVNQILQQLSDGLLYVNSDGGPVLLQGGTDDEPTYSDPITGEAAADVDPDM
HHHHHCCCCCHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHH
MTKVKINNALRGVISAATSQLTLMSPDRSARLAAAQGLLKDADPANLDLLNAALAAEKDA
HHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCH
EIKNTMEAARAVLLLKTDASVEDKKAAIDTIAARGNRDALTILTTTLETAPDDLKPAIQA
HHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCHHHHHHHH
DISTINRSLALWDVAQNVWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMIGAYTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHH
YVVQEYITSAFPGVADYSLAFAVPAAFVFTGFVGLVIERAVIRYLYGRPLETLLATWGVS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LILQQAVRSIFGPTNREVRNPSWMSGVFDLGGLSITWNRLWIIVFSMVVFVMLLLLLKRS
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCH
AFGLQMRAVTQNRRMASSMGIRTGWVDAFTFALGSGIAGIAGVALSQIDNVSPNLGQSYI
HCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
IDSFMVVVFGGVGNLWGTLVGALSLGVVNKFLEPFAGAVLGKILVLVLIILFIQKRPRGL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FALKGRAVEA
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]