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The map label for this gene is rutR [H]

Identifier: 209550611

GI number: 209550611

Start: 3094813

End: 3095490

Strand: Reverse

Name: rutR [H]

Synonym: Rleg2_3035

Alternate gene names: 209550611

Gene position: 3095490-3094813 (Counterclockwise)

Preceding gene: 209550615

Following gene: 209550607

Centisome position: 68.21

GC content: 61.06

Gene sequence:

>678_bases
TTGAATTTCAAGGGGAAACGACAGCCTGTGACCATACCGAGAGCAGCGAAAACCCTGAGGCGGACGCGAATTCAGGAGGA
GAAGGAAGAGCAGATCCTGGAGGCGGCGCTCGACGTGTTTTCGGCAAGCGGCTTTCGCGGCTCGACCATCGACCAGATCG
CCGAAGTGGCCGGCATGTCGAAACCCAACCTGCTCTATTATTTCCGCACCAAGGAAGCGATGCATCGGGCGCTGATCGAC
CGGGTGCTCTATACTTGGCTGGAGCCGCTGCGTGCCTTCGACGCCGAGGGCAATCCGGAAGAGGAAATCCGCAGCTACAT
CAGACGCAAGCTGGAGATGGCCCGCGATTTTCCGCGTGAAAGCCGGCTCTTCGCCAATGAGGTGCTGCAGGGCGCGCCGC
ATATCGAGGACGAGCTGAAGGGGCCGCTGAAGGAACTGGTCGACGAGAAGGCGGAGGTCATCCGCGCCTGGACGAAATCC
GGCAAGATCGCCAAATGTGATCCCTACCACCTGATCTTCTCCATCTGGTCGACGACGCAGCATTACGCGGATTTCGACGT
GCAGGTGCGTGCGGTGCTCGGGCAGGAGCATTCCGGCGAGGGGCGCTTCGAGGATGCGGCGCGGTTTCTGGAACAGCTCT
TCATCGGCGGGCTGCGCCCGGATCGCTCTGAGAGCTGA

Upstream 100 bases:

>100_bases
CCGGGCTCAAGACGAAAATCACGAAAATTGCCGATAAAAATACGGGCAGTTGCTCAAGAATTCGGCAGGGTCGCCTCCTG
CCAAAACTTGAGCGAAGAAG

Downstream 100 bases:

>100_bases
CGTTTATTGCTTGTTCAAAACGGGCAGGCGAGCGAGGGTGTCCGTCACACCGTCCATCGTATAGGGCTTCTGCAGCACGG
GCGCGTCCGAGTGGCTGTTT

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MNFKGKRQPVTIPRAAKTLRRTRIQEEKEEQILEAALDVFSASGFRGSTIDQIAEVAGMSKPNLLYYFRTKEAMHRALID
RVLYTWLEPLRAFDAEGNPEEEIRSYIRRKLEMARDFPRESRLFANEVLQGAPHIEDELKGPLKELVDEKAEVIRAWTKS
GKIAKCDPYHLIFSIWSTTQHYADFDVQVRAVLGQEHSGEGRFEDAARFLEQLFIGGLRPDRSES

Sequences:

>Translated_225_residues
MNFKGKRQPVTIPRAAKTLRRTRIQEEKEEQILEAALDVFSASGFRGSTIDQIAEVAGMSKPNLLYYFRTKEAMHRALID
RVLYTWLEPLRAFDAEGNPEEEIRSYIRRKLEMARDFPRESRLFANEVLQGAPHIEDELKGPLKELVDEKAEVIRAWTKS
GKIAKCDPYHLIFSIWSTTQHYADFDVQVRAVLGQEHSGEGRFEDAARFLEQLFIGGLRPDRSES
>Mature_225_residues
MNFKGKRQPVTIPRAAKTLRRTRIQEEKEEQILEAALDVFSASGFRGSTIDQIAEVAGMSKPNLLYYFRTKEAMHRALID
RVLYTWLEPLRAFDAEGNPEEEIRSYIRRKLEMARDFPRESRLFANEVLQGAPHIEDELKGPLKELVDEKAEVIRAWTKS
GKIAKCDPYHLIFSIWSTTQHYADFDVQVRAVLGQEHSGEGRFEDAARFLEQLFIGGLRPDRSES

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=203, Percent_Identity=50.7389162561576, Blast_Score=210, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 25956; Mature: 25956

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKGKRQPVTIPRAAKTLRRTRIQEEKEEQILEAALDVFSASGFRGSTIDQIAEVAGMS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
KPNLLYYFRTKEAMHRALIDRVLYTWLEPLRAFDAEGNPEEEIRSYIRRKLEMARDFPRE
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHH
SRLFANEVLQGAPHIEDELKGPLKELVDEKAEVIRAWTKSGKIAKCDPYHLIFSIWSTTQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHH
HYADFDVQVRAVLGQEHSGEGRFEDAARFLEQLFIGGLRPDRSES
HHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNFKGKRQPVTIPRAAKTLRRTRIQEEKEEQILEAALDVFSASGFRGSTIDQIAEVAGMS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
KPNLLYYFRTKEAMHRALIDRVLYTWLEPLRAFDAEGNPEEEIRSYIRRKLEMARDFPRE
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHH
SRLFANEVLQGAPHIEDELKGPLKELVDEKAEVIRAWTKSGKIAKCDPYHLIFSIWSTTQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHH
HYADFDVQVRAVLGQEHSGEGRFEDAARFLEQLFIGGLRPDRSES
HHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]