Click here to switch to the map view.

The map label for this gene is trpE(G) [H]

Identifier: 209550391

GI number: 209550391

Start: 2856213

End: 2858402

Strand: Reverse

Name: trpE(G) [H]

Synonym: Rleg2_2812

Alternate gene names: 209550391

Gene position: 2858402-2856213 (Counterclockwise)

Preceding gene: 209550392

Following gene: 209550390

Centisome position: 62.99

GC content: 62.37

Gene sequence:

>2190_bases
ATGGTAACGATCCTGCGGGATGATGGTGCGGAAATCTACGAGACCAAGGGCGGGATATCGGTCACGCGGCAGCGGCGTGC
GATCCCCTACGGCGACGCGGTCTCTTCCTATATCGACAAGCTCGATGAGCGCCGCGGCGCGGTGTTTTCGTCGAACTACG
AATATCCGGGACGTTATACGCGCTGGGATACCGCCGTCGTCGATCCGCCGCTCGGCATCTCCTCCTTCGGGCGCAACGTC
TGGATCGAAGCCTATAACGAACGCGGCGAGGTACTTCTCGGCTTCGTGACCGAGCGGCTGAAAACGGTGTCCGATCTCGT
GCTCGGCGCTTCCTCCGCCCGCCGTCTCGACCTCTCGGTCAAAGCGCCGGATCGGGTGTTCACCGAGGAAGAGCGCTCGA
AGATGCCGACGGTCTTTACCGTGCTGCGTGCCGTCACCGACCTCTTCTATTCGCAGGCGGATGCGAGCCTTGGGCTTTAC
GGCGCCTTCGGCTATGACATCGCCTTCCAGTTCGATGCAATCGATCTGAAGCTGACCCGGCCCTCCGACCAGCGCGATAT
GGTGCTCTACCTGCCGGACGAAATCCTCGTCGTCGACAACTATGCCGCCAAGGCCTGGATCGACCGTTACGATTTCGAAA
AGGGCGGCGTGACGACCGAGGGCAAGGCGCAAGACATCGCGCCGGAGCCTTTCAAGCAAACGGATGCCATTCCCCCGAAG
AGCGATCACCGGCCGGGCGAATATGCCGATCTCGTCGTCAAGGCGAAGGAAAGTTTCCGCAAGGGCGATCTCTTCGAAGT
CGTGCCCGGGCAGAAATTCATGGAGCGCTGCGAAAGCAAGCCTTCCGACATTTCCAAGCGGCTGAAGGCGATCAATCCGT
CTCCCTATTCCTTCTTCATCAATCTCGGCCATCAGGAATATCTGGTCGGTGCCTCGCCCGAGATGTTCGTGCGCGTGTCC
GGCCGCCGGATCGAGACCTGCCCGATCTCGGGCACGATCAAGCGCGGTGACGACCCGATCGCCGACAGCGAGCAGATCCT
GAAGCTCTTGAATTCCAAGAAGGACGAATCCGAGCTGACCATGTGCTCCGACGTCGACCGCAACGACAAGAGCCGCGTCT
GCGAGCCTGGCTCGGTCAAGGTGATCGGCCGCCGGCAGATCGAGATGTATTCGCGCCTCATCCACACGGTCGACCATATC
GAGGGCCGGCTACGCGACGATATGGACGCCTTCGACGGGTTCTTGAGCCATGCCTGGGCCGTGACCGTCACCGGCGCTCC
GAAGCTCTGGGCGATGCGCTTTATCGAGAGCCGTGAAAAGAGCCCGCGCGCATGGTATGGTGGAGCGATCGGCATGGTCG
GCTTCAACGGCGACATGAACACCGGCCTGACGCTGCGCACGGTGCGCATCAAGGACGGTATCGCCGAGGTACGCGCCGGG
GCGACGCTGCTCAACGATTCCATTCCTGAAGAAGAAGAAGCCGAAACAGAATTGAAGGCCTCTGCCATGCTTTCCGCCAT
CCGCGACGCCAAGACCGGCAATTCCGGCAAGACCCAGCGCGATGTCGCAAGCGTCGGCAAGGGCGTTAACATCCTGCTCG
TCGACCATGAGGACAGCTTCGTCCACACGCTTGCCAATTATTTCCGCCAGACGGGGGCGAGCGTTTCGACCGTGCGCACG
CCGGTGCCGGAGGAAATCTTCGACCGGCTGAACCCGGACCTCGTCGTGCTGTCGCCCGGGCCTGGAACGCCCAAGGATTT
CGACTGCAAGGCGACGATTAAGAAAGCGCGGGCGCGCAACCTGCCGATCTTCGGCGTCTGCCTCGGCCTGCAGGCGCTCG
CCGAGGCCTATGGCGGGGAACTGCGCCATCTGGCGCTGCCGATGCACGGCAAACCCTCGCGCATCCGCGTGCTGGAGCCG
GGCATCGTCTTCTCCGGCCTGTCGAAGGAGGTGACGGTCGGCCGCTACCACTCGATCTTCGCCGATCCCTCGACGCTGCC
GCGCGACTTCATCATCACCGCGGAAAGCGAAGACGGCACGATCATGGGCATCGAGCACGCCAAGGAGCCGATCGCCGCGG
TGCAGTTCCACCCGGAATCGATCATGACGCTCGGCGGCGATGCTGGCATGCGGATGATCGAAAATGTCGTGGCGCATCTG
GCCCGCAAGGCGAAGACCAAGGCTGCCTGA

Upstream 100 bases:

>100_bases
TCGTGTCCAAAGACGACGACGAGTGAGCCGCCCGAAACTTCGAGGCGGCTTTTTTATTTATCGCCGCCTGTCGTTCGGGC
CCCGATAACGGAGTGGAACT

Downstream 100 bases:

>100_bases
GCGCAACTCGGCCTTGCCCTTCCTGCTTGCGGGAAGGGCGGGCGAGGTGTGCATTCCGACGTGGATACGACGAGCAGCGA
AGGAACGCGGGCAAGCATGG

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 729; Mature: 729

Protein sequence:

>729_residues
MVTILRDDGAEIYETKGGISVTRQRRAIPYGDAVSSYIDKLDERRGAVFSSNYEYPGRYTRWDTAVVDPPLGISSFGRNV
WIEAYNERGEVLLGFVTERLKTVSDLVLGASSARRLDLSVKAPDRVFTEEERSKMPTVFTVLRAVTDLFYSQADASLGLY
GAFGYDIAFQFDAIDLKLTRPSDQRDMVLYLPDEILVVDNYAAKAWIDRYDFEKGGVTTEGKAQDIAPEPFKQTDAIPPK
SDHRPGEYADLVVKAKESFRKGDLFEVVPGQKFMERCESKPSDISKRLKAINPSPYSFFINLGHQEYLVGASPEMFVRVS
GRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHI
EGRLRDDMDAFDGFLSHAWAVTVTGAPKLWAMRFIESREKSPRAWYGGAIGMVGFNGDMNTGLTLRTVRIKDGIAEVRAG
ATLLNDSIPEEEEAETELKASAMLSAIRDAKTGNSGKTQRDVASVGKGVNILLVDHEDSFVHTLANYFRQTGASVSTVRT
PVPEEIFDRLNPDLVVLSPGPGTPKDFDCKATIKKARARNLPIFGVCLGLQALAEAYGGELRHLALPMHGKPSRIRVLEP
GIVFSGLSKEVTVGRYHSIFADPSTLPRDFIITAESEDGTIMGIEHAKEPIAAVQFHPESIMTLGGDAGMRMIENVVAHL
ARKAKTKAA

Sequences:

>Translated_729_residues
MVTILRDDGAEIYETKGGISVTRQRRAIPYGDAVSSYIDKLDERRGAVFSSNYEYPGRYTRWDTAVVDPPLGISSFGRNV
WIEAYNERGEVLLGFVTERLKTVSDLVLGASSARRLDLSVKAPDRVFTEEERSKMPTVFTVLRAVTDLFYSQADASLGLY
GAFGYDIAFQFDAIDLKLTRPSDQRDMVLYLPDEILVVDNYAAKAWIDRYDFEKGGVTTEGKAQDIAPEPFKQTDAIPPK
SDHRPGEYADLVVKAKESFRKGDLFEVVPGQKFMERCESKPSDISKRLKAINPSPYSFFINLGHQEYLVGASPEMFVRVS
GRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHI
EGRLRDDMDAFDGFLSHAWAVTVTGAPKLWAMRFIESREKSPRAWYGGAIGMVGFNGDMNTGLTLRTVRIKDGIAEVRAG
ATLLNDSIPEEEEAETELKASAMLSAIRDAKTGNSGKTQRDVASVGKGVNILLVDHEDSFVHTLANYFRQTGASVSTVRT
PVPEEIFDRLNPDLVVLSPGPGTPKDFDCKATIKKARARNLPIFGVCLGLQALAEAYGGELRHLALPMHGKPSRIRVLEP
GIVFSGLSKEVTVGRYHSIFADPSTLPRDFIITAESEDGTIMGIEHAKEPIAAVQFHPESIMTLGGDAGMRMIENVVAHL
ARKAKTKAA
>Mature_729_residues
MVTILRDDGAEIYETKGGISVTRQRRAIPYGDAVSSYIDKLDERRGAVFSSNYEYPGRYTRWDTAVVDPPLGISSFGRNV
WIEAYNERGEVLLGFVTERLKTVSDLVLGASSARRLDLSVKAPDRVFTEEERSKMPTVFTVLRAVTDLFYSQADASLGLY
GAFGYDIAFQFDAIDLKLTRPSDQRDMVLYLPDEILVVDNYAAKAWIDRYDFEKGGVTTEGKAQDIAPEPFKQTDAIPPK
SDHRPGEYADLVVKAKESFRKGDLFEVVPGQKFMERCESKPSDISKRLKAINPSPYSFFINLGHQEYLVGASPEMFVRVS
GRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHI
EGRLRDDMDAFDGFLSHAWAVTVTGAPKLWAMRFIESREKSPRAWYGGAIGMVGFNGDMNTGLTLRTVRIKDGIAEVRAG
ATLLNDSIPEEEEAETELKASAMLSAIRDAKTGNSGKTQRDVASVGKGVNILLVDHEDSFVHTLANYFRQTGASVSTVRT
PVPEEIFDRLNPDLVVLSPGPGTPKDFDCKATIKKARARNLPIFGVCLGLQALAEAYGGELRHLALPMHGKPSRIRVLEP
GIVFSGLSKEVTVGRYHSIFADPSTLPRDFIITAESEDGTIMGIEHAKEPIAAVQFHPESIMTLGGDAGMRMIENVVAHL
ARKAKTKAA

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=475, Percent_Identity=30.1052631578947, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1788114, Length=364, Percent_Identity=29.9450549450549, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI1789760, Length=188, Percent_Identity=42.5531914893617, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1787517, Length=199, Percent_Identity=36.6834170854271, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1786809, Length=225, Percent_Identity=29.3333333333333, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1786215, Length=151, Percent_Identity=31.7880794701987, Blast_Score=76, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=272, Percent_Identity=37.8676470588235, Blast_Score=160, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6322638, Length=191, Percent_Identity=39.2670157068063, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR001317
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 80606; Mature: 80606

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTILRDDGAEIYETKGGISVTRQRRAIPYGDAVSSYIDKLDERRGAVFSSNYEYPGRYT
CEEEEECCCCEEEECCCCCEEHHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCC
RWDTAVVDPPLGISSFGRNVWIEAYNERGEVLLGFVTERLKTVSDLVLGASSARRLDLSV
CCCCEECCCCCCHHHCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEE
KAPDRVFTEEERSKMPTVFTVLRAVTDLFYSQADASLGLYGAFGYDIAFQFDAIDLKLTR
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEEEEEEEEEEEC
PSDQRDMVLYLPDEILVVDNYAAKAWIDRYDFEKGGVTTEGKAQDIAPEPFKQTDAIPPK
CCCCCCEEEEECCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC
SDHRPGEYADLVVKAKESFRKGDLFEVVPGQKFMERCESKPSDISKRLKAINPSPYSFFI
CCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCHHHHHHHHHHCCCCCEEEEE
NLGHQEYLVGASPEMFVRVSGRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELT
ECCCEEEEECCCCCEEEEECCCEEEECCCCCEEECCCCCCCCHHHHHHHHHCCCCCCHHH
MCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHIEGRLRDDMDAFDGFLSHAWA
EHHCCCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VTVTGAPKLWAMRFIESREKSPRAWYGGAIGMVGFNGDMNTGLTLRTVRIKDGIAEVRAG
EEECCCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHHHHCC
ATLLNDSIPEEEEAETELKASAMLSAIRDAKTGNSGKTQRDVASVGKGVNILLVDHEDSF
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCCHH
VHTLANYFRQTGASVSTVRTPVPEEIFDRLNPDLVVLSPGPGTPKDFDCKATIKKARARN
HHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
LPIFGVCLGLQALAEAYGGELRHLALPMHGKPSRIRVLEPGIVFSGLSKEVTVGRYHSIF
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCEEECCCCCCEECCHHHEEE
ADPSTLPRDFIITAESEDGTIMGIEHAKEPIAAVQFHPESIMTLGGDAGMRMIENVVAHL
CCCCCCCCEEEEEEECCCCEEEEHHHCCCCCEEEEECCHHHEEECCHHHHHHHHHHHHHH
ARKAKTKAA
HHHHHCCCC
>Mature Secondary Structure
MVTILRDDGAEIYETKGGISVTRQRRAIPYGDAVSSYIDKLDERRGAVFSSNYEYPGRYT
CEEEEECCCCEEEECCCCCEEHHHCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCC
RWDTAVVDPPLGISSFGRNVWIEAYNERGEVLLGFVTERLKTVSDLVLGASSARRLDLSV
CCCCEECCCCCCHHHCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEE
KAPDRVFTEEERSKMPTVFTVLRAVTDLFYSQADASLGLYGAFGYDIAFQFDAIDLKLTR
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEEEEEEEEEEEEEC
PSDQRDMVLYLPDEILVVDNYAAKAWIDRYDFEKGGVTTEGKAQDIAPEPFKQTDAIPPK
CCCCCCEEEEECCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC
SDHRPGEYADLVVKAKESFRKGDLFEVVPGQKFMERCESKPSDISKRLKAINPSPYSFFI
CCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCHHHHHHHHHHCCCCCEEEEE
NLGHQEYLVGASPEMFVRVSGRRIETCPISGTIKRGDDPIADSEQILKLLNSKKDESELT
ECCCEEEEECCCCCEEEEECCCEEEECCCCCEEECCCCCCCCHHHHHHHHHCCCCCCHHH
MCSDVDRNDKSRVCEPGSVKVIGRRQIEMYSRLIHTVDHIEGRLRDDMDAFDGFLSHAWA
EHHCCCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VTVTGAPKLWAMRFIESREKSPRAWYGGAIGMVGFNGDMNTGLTLRTVRIKDGIAEVRAG
EEECCCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHHHHCC
ATLLNDSIPEEEEAETELKASAMLSAIRDAKTGNSGKTQRDVASVGKGVNILLVDHEDSF
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEECCCHH
VHTLANYFRQTGASVSTVRTPVPEEIFDRLNPDLVVLSPGPGTPKDFDCKATIKKARARN
HHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
LPIFGVCLGLQALAEAYGGELRHLALPMHGKPSRIRVLEPGIVFSGLSKEVTVGRYHSIF
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCEEECCCCCCEECCHHHEEE
ADPSTLPRDFIITAESEDGTIMGIEHAKEPIAAVQFHPESIMTLGGDAGMRMIENVVAHL
CCCCCCCCEEEEEEECCCCEEEEHHHCCCCCEEEEECCHHHEEECCHHHHHHHHHHHHHH
ARKAKTKAA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656657; 11481430 [H]