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The map label for this gene is potH [H]

Identifier: 209550237

GI number: 209550237

Start: 2705173

End: 2706078

Strand: Reverse

Name: potH [H]

Synonym: Rleg2_2657

Alternate gene names: 209550237

Gene position: 2706078-2705173 (Counterclockwise)

Preceding gene: 209550238

Following gene: 209550236

Centisome position: 59.63

GC content: 60.6

Gene sequence:

>906_bases
ATGGCAAGCATCGCAGCAGACACGGAGGAAGGGCGGCAGGCCGGGCGCAGACTGAGCCTCTCCCCCTCGGCCATCTCCTA
TCTCCAGGCGACGCCGCTGATCGCCATTCTCGGCTTCTTCTTCCTGCTGCCGATCGCCATGATCGCTGTCGTCAGTTTCT
GGGATTATGATTTCGCCGGCCTCTACCCCGATTTCCTCACCATGAACTACACCGATACGCTCGGTTCGTGGGTGACGTGG
AAGACCTATCTCAACACGTTGAAATTCACCGCGATCGTCTGGGCGCTGACGCTTCTCATCGGTTTCTGGGTCGCCTATTT
CCTGGCCTTCCACATCCGCAAGACCTCGACGCAGATGATCCTCTTTCTCGTCTGCACCGTGCCGTTCATGACCTCGAACA
TCATCCGCATGATCTCGTGGATCCCGGTGCTCGGGCGCAACGGCCTCGTCAATTCGACGCTGATCGAGTTGGGCATCATC
CCGCAGCCGATCGAATGGCTGCTCTATTCCGATTTCGCCGTCGTGCTCGCCATGGTGCATCTCTATACGCTGTTCATGGT
GACGCCGATCTTCAACACGCTGATGCGCATCGATCGGTCGCTGTTCGAGGCGGCGCGCGATGCCGGCGCCTCCGGCTGGC
AGGTGCTGTGGAATGTCGTCATTCCGCTGGCAAAACCCGGCATGGCGATCGGCACGATCTTCGTCGTCACCCTCGTCATG
GCCGATTTCTCGACGGTGCAGGTGATGTCCGGCGGCCAGAGCGCCTCGGTGGCGCTGATGATGAAGAACCAGATGTCGCT
GCTGCAATATCCGGCGGCCGCCGCCAACGCCGTGGTGCTGCTGGCCGTCGTGCTGCTGATGGTCGCGGCCATTCTGCGCG
TCGTCGACATCCGCAAGGAACTTTGA

Upstream 100 bases:

>100_bases
CGAGACGAGTGGCTCGACCCAGGTAAAGGTGCCGGCAGGCGGATGAGGGGGCGGCACGCACCTTCGTTCAACCTGACGTA
ATCAAAACGGAGAACCGGAT

Downstream 100 bases:

>100_bases
GATGAACCACGAAAAACGCGGTCTCGAATTCTATCTGCTGGCGATCTTCTTCGCCGTTTTCGTGCTGTTCCTCTACGGTC
CGCTGTCGGCGGTGCTGATC

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MASIAADTEEGRQAGRRLSLSPSAISYLQATPLIAILGFFFLLPIAMIAVVSFWDYDFAGLYPDFLTMNYTDTLGSWVTW
KTYLNTLKFTAIVWALTLLIGFWVAYFLAFHIRKTSTQMILFLVCTVPFMTSNIIRMISWIPVLGRNGLVNSTLIELGII
PQPIEWLLYSDFAVVLAMVHLYTLFMVTPIFNTLMRIDRSLFEAARDAGASGWQVLWNVVIPLAKPGMAIGTIFVVTLVM
ADFSTVQVMSGGQSASVALMMKNQMSLLQYPAAAANAVVLLAVVLLMVAAILRVVDIRKEL

Sequences:

>Translated_301_residues
MASIAADTEEGRQAGRRLSLSPSAISYLQATPLIAILGFFFLLPIAMIAVVSFWDYDFAGLYPDFLTMNYTDTLGSWVTW
KTYLNTLKFTAIVWALTLLIGFWVAYFLAFHIRKTSTQMILFLVCTVPFMTSNIIRMISWIPVLGRNGLVNSTLIELGII
PQPIEWLLYSDFAVVLAMVHLYTLFMVTPIFNTLMRIDRSLFEAARDAGASGWQVLWNVVIPLAKPGMAIGTIFVVTLVM
ADFSTVQVMSGGQSASVALMMKNQMSLLQYPAAAANAVVLLAVVLLMVAAILRVVDIRKEL
>Mature_300_residues
ASIAADTEEGRQAGRRLSLSPSAISYLQATPLIAILGFFFLLPIAMIAVVSFWDYDFAGLYPDFLTMNYTDTLGSWVTWK
TYLNTLKFTAIVWALTLLIGFWVAYFLAFHIRKTSTQMILFLVCTVPFMTSNIIRMISWIPVLGRNGLVNSTLIELGIIP
QPIEWLLYSDFAVVLAMVHLYTLFMVTPIFNTLMRIDRSLFEAARDAGASGWQVLWNVVIPLAKPGMAIGTIFVVTLVMA
DFSTVQVMSGGQSASVALMMKNQMSLLQYPAAAANAVVLLAVVLLMVAAILRVVDIRKEL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=211, Percent_Identity=34.5971563981043, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787369, Length=251, Percent_Identity=31.0756972111554, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787713, Length=223, Percent_Identity=27.8026905829596, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33369; Mature: 33238

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIAADTEEGRQAGRRLSLSPSAISYLQATPLIAILGFFFLLPIAMIAVVSFWDYDFAG
CCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LYPDFLTMNYTDTLGSWVTWKTYLNTLKFTAIVWALTLLIGFWVAYFLAFHIRKTSTQMI
CCHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFLVCTVPFMTSNIIRMISWIPVLGRNGLVNSTLIELGIIPQPIEWLLYSDFAVVLAMVH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LYTLFMVTPIFNTLMRIDRSLFEAARDAGASGWQVLWNVVIPLAKPGMAIGTIFVVTLVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ADFSTVQVMSGGQSASVALMMKNQMSLLQYPAAAANAVVLLAVVLLMVAAILRVVDIRKE
HCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure 
ASIAADTEEGRQAGRRLSLSPSAISYLQATPLIAILGFFFLLPIAMIAVVSFWDYDFAG
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]