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The map label for this gene is rna [H]

Identifier: 209550201

GI number: 209550201

Start: 2665458

End: 2666198

Strand: Reverse

Name: rna [H]

Synonym: Rleg2_2621

Alternate gene names: 209550201

Gene position: 2666198-2665458 (Counterclockwise)

Preceding gene: 209550206

Following gene: 209550200

Centisome position: 58.75

GC content: 63.83

Gene sequence:

>741_bases
ATGAGCAGCATGCAGTTGCGTACCTTGACGTTCGCCGTATCGATGGTTCTTGCCGGCGCCGCCGTGGCGCAGGAGGCTGG
CGGGCGCACCCGTTTTATTCTTTCGGCGAGCTGGCAGCCGGCTTTTTGCCAGACGAACCAGAAGAAGGCCGAATGTGCAA
GCCAGACCGGTGAGCGCGCCGATGCGACGAATTTTTCGCTGCACGGGCTTTGGCCAATGCGTCAGAACTATTGCGATGTC
AGTGCCGAGCAGAAGGCTGCCGACAAGGACGGCAAGTGGGACACATTGCCGGCGGTCACGCTGTCGGCCGAGACGCAGGC
CGCGCTGGCAAAGGCGATGCCCGGCACGCAATCCGGCCTGGAACGGCATGAATGGGTAAAGCACGGCACCTGCACGAAAA
TGAGCGCCGACGATTATTTCGGCGTCGGCGTGCATCTTGTCGGCGCGCTTAACAGCTCCGCGGTGCGTGATCTCTTCGCC
GCCAATATCGGCAAGCCGGTCAAGGCCGAGGCGATCAAGGAAGCTTTCGACAAGAGCTTCGGGCCGGGTGCCGGCGACCG
CGTCAAGATGAGCTGCCGGCGGGCCGGCAAGGTCAAGATGATCAGCGGGTTGACCATCGGGCTTTCGGAGGCCGCCGGGG
CGGCGTCGGCGAAGAGCGCCGGCCTTGGCGATCTGATCCAGGGCGCGGGAAAAACGACGTTCGGATGCGACGAGGGCGTC
GTCGACGCAGCGGGCTTCTGA

Upstream 100 bases:

>100_bases
GTTTATCCGATAACAAATTGCGTTTCTGCTGCGATTCAGGGTTGCGGGCTTTCGATCTTCGTGCTTATTTGTTCCTGTAT
TGTTCTTTTTGGGGGTTGAA

Downstream 100 bases:

>100_bases
CCGGAAATCCTGCGTAAACCGGGTTTCGGCGTTGCCCGCAGGCGCTGTTTCGCCTACATAGCGGCACAATCGAAGTTTCC
CGCCCGGAGCAGCGCAGCAT

Product: ribonuclease T2

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSSMQLRTLTFAVSMVLAGAAVAQEAGGRTRFILSASWQPAFCQTNQKKAECASQTGERADATNFSLHGLWPMRQNYCDV
SAEQKAADKDGKWDTLPAVTLSAETQAALAKAMPGTQSGLERHEWVKHGTCTKMSADDYFGVGVHLVGALNSSAVRDLFA
ANIGKPVKAEAIKEAFDKSFGPGAGDRVKMSCRRAGKVKMISGLTIGLSEAAGAASAKSAGLGDLIQGAGKTTFGCDEGV
VDAAGF

Sequences:

>Translated_246_residues
MSSMQLRTLTFAVSMVLAGAAVAQEAGGRTRFILSASWQPAFCQTNQKKAECASQTGERADATNFSLHGLWPMRQNYCDV
SAEQKAADKDGKWDTLPAVTLSAETQAALAKAMPGTQSGLERHEWVKHGTCTKMSADDYFGVGVHLVGALNSSAVRDLFA
ANIGKPVKAEAIKEAFDKSFGPGAGDRVKMSCRRAGKVKMISGLTIGLSEAAGAASAKSAGLGDLIQGAGKTTFGCDEGV
VDAAGF
>Mature_245_residues
SSMQLRTLTFAVSMVLAGAAVAQEAGGRTRFILSASWQPAFCQTNQKKAECASQTGERADATNFSLHGLWPMRQNYCDVS
AEQKAADKDGKWDTLPAVTLSAETQAALAKAMPGTQSGLERHEWVKHGTCTKMSADDYFGVGVHLVGALNSSAVRDLFAA
NIGKPVKAEAIKEAFDKSFGPGAGDRVKMSCRRAGKVKMISGLTIGLSEAAGAASAKSAGLGDLIQGAGKTTFGCDEGVV
DAAGF

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=248, Percent_Identity=29.4354838709677, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 25642; Mature: 25511

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSMQLRTLTFAVSMVLAGAAVAQEAGGRTRFILSASWQPAFCQTNQKKAECASQTGERA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHHHHHHHCCCCC
DATNFSLHGLWPMRQNYCDVSAEQKAADKDGKWDTLPAVTLSAETQAALAKAMPGTQSGL
CCCCCEEEECCHHHHCCCCCCHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHH
ERHEWVKHGTCTKMSADDYFGVGVHLVGALNSSAVRDLFAANIGKPVKAEAIKEAFDKSF
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
GPGAGDRVKMSCRRAGKVKMISGLTIGLSEAAGAASAKSAGLGDLIQGAGKTTFGCDEGV
CCCCCCHHHHHHHHCCCEEEECCEEEEHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCC
VDAAGF
CCCCCC
>Mature Secondary Structure 
SSMQLRTLTFAVSMVLAGAAVAQEAGGRTRFILSASWQPAFCQTNQKKAECASQTGERA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCHHHHHHHHCCCCC
DATNFSLHGLWPMRQNYCDVSAEQKAADKDGKWDTLPAVTLSAETQAALAKAMPGTQSGL
CCCCCEEEECCHHHHCCCCCCHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHH
ERHEWVKHGTCTKMSADDYFGVGVHLVGALNSSAVRDLFAANIGKPVKAEAIKEAFDKSF
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
GPGAGDRVKMSCRRAGKVKMISGLTIGLSEAAGAASAKSAGLGDLIQGAGKTTFGCDEGV
CCCCCCHHHHHHHHCCCEEEECCEEEEHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCC
VDAAGF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]