Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 209550176

GI number: 209550176

Start: 2636070

End: 2637482

Strand: Reverse

Name: murD [H]

Synonym: Rleg2_2596

Alternate gene names: 209550176

Gene position: 2637482-2636070 (Counterclockwise)

Preceding gene: 209550177

Following gene: 209550175

Centisome position: 58.12

GC content: 65.53

Gene sequence:

>1413_bases
ATGATCCCGGTCACGACGCTCAAGGACAGGAAGGTCGCGCTCTTCGGGCTCGGCGGCTCGGGCCTTGCCACCGCCCGGGC
GCTGGTTTCGGGCGGCGCCGAGGTGACCGCCTGGGACGACAATCCCGACAGCGTCGCCAAGGCTTCCGCCGAAGGCATCC
GCACCGAGGACCTGCACACCATCGACTGGAGCCAGCAGGCGCTGTTCGTGCTCTCGCCCGGCGTGCCGCTCACCCATCCG
AAGCCGCACTGGACCGTCGATCTTGCGCGCGCCGCCGGTGTCGATATCGTCGGCGATGTCGAGCTCTTCGTGCGCGAGCG
CCGCGCCCATGCGCCGGATTGCCCGTTCATCGCCATCACGGGCACTAACGGCAAATCGACGACGACGGCGCTGATTGCCC
ATATCCTGAAATCCGCAGGCTACGATACGCAGCTCGGCGGCAATATCGGCACCGCGGTTCTGACGCTCGATCCGCCGAAG
GCCGGGCGTTATTACGTCGTCGAATGCTCCTCCTACCAGATCGATCTGGCGCCGACGCTGAACCCTTCCGCCGGCATCCT
GCTCAACCTGACGCCCGATCATCTCGACCGTCACGGCACGATGCAGCATTACGCCGATGTCAAGGAACGGCTGGTTGCCG
GCAGCGATGTGGCAATCGTCGGCGTCGACGACAGCCATTCGGCGCTGATTGCCGACCGCGTCGAGCGGGCGGATGTCAAG
GTGGTGCGCATTTCGCGCCGCAGCGTGGTGGCATCAGGCATTTATGCCGAGGGCAGCAGGCTCATTCAGGCCGCCGGCGG
CGCCATGCTGCCCTTCGCCGATCTCGATGGTATCCAGACGCTGCGCGGCAGCCATAATGCGCAGAACGCCGCCGCAGCTG
TCGCCGCCTGCCTTGCCGTCGGTGTTTCCGCCGACGACATCCGCGCCGGCCTTGCCTCGTTCCCCGGCCTCAAGCACCGC
ATGCAGCCGGTGGGGCAGCGAGGCCGCGTCGTTTTCGTCAATGATTCCAAGGCGACCAATGCCGATGCTGCGGCACCGGC
GCTTTCGAGCTATGATCGCATCTACTGGATTGCCGGTGGCCTGCCAAAGGCCGGCGGCATCACCACGCTCGCTCCCTATT
TTCCACGCATCGCCAAGGCCTATCTGATCGGCGAGGCGGCGGCGGAATTTGCAGCGACGCTCGGCGAGGCCGTGCCTTAC
GAGATCTCGGGCACGCTGGAGCGCGCGGTCGTGCATGCGGCGGCCGATGCCGAGCGCGACGAGGCCGCCGCTTCGGCCGT
CATGTTATCCCCGGCTTGCGCAAGCTTCGATCAGTATAAGAATTTCGAAGTCAGGGGTGAAGCCTTCGTCGGACACGTGG
CAGCGCTTGACGGGATTGCGATGCTGATCGGTTCGGCAACAGGAGAGAAATGA

Upstream 100 bases:

>100_bases
AAGGGCTGGACCGAAAGCCAGGTGGTGATCCGCTTCTGGATCATCGCCGTCGGCCTTGCCATGCTCGGCCTCTCGACGCT
GAAGCTGCGGTGAGGCGGCG

Downstream 100 bases:

>100_bases
CATGGTAAGCCGCGCGGAACGTGGGCCTCTGGCCGATTGGTTCTGGACCATCGACCGCTTCTTCCTGGCGATGTTCATCT
TCCTGATGGGCATCGGCTTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MIPVTTLKDRKVALFGLGGSGLATARALVSGGAEVTAWDDNPDSVAKASAEGIRTEDLHTIDWSQQALFVLSPGVPLTHP
KPHWTVDLARAAGVDIVGDVELFVRERRAHAPDCPFIAITGTNGKSTTTALIAHILKSAGYDTQLGGNIGTAVLTLDPPK
AGRYYVVECSSYQIDLAPTLNPSAGILLNLTPDHLDRHGTMQHYADVKERLVAGSDVAIVGVDDSHSALIADRVERADVK
VVRISRRSVVASGIYAEGSRLIQAAGGAMLPFADLDGIQTLRGSHNAQNAAAAVAACLAVGVSADDIRAGLASFPGLKHR
MQPVGQRGRVVFVNDSKATNADAAAPALSSYDRIYWIAGGLPKAGGITTLAPYFPRIAKAYLIGEAAAEFAATLGEAVPY
EISGTLERAVVHAAADAERDEAAASAVMLSPACASFDQYKNFEVRGEAFVGHVAALDGIAMLIGSATGEK

Sequences:

>Translated_470_residues
MIPVTTLKDRKVALFGLGGSGLATARALVSGGAEVTAWDDNPDSVAKASAEGIRTEDLHTIDWSQQALFVLSPGVPLTHP
KPHWTVDLARAAGVDIVGDVELFVRERRAHAPDCPFIAITGTNGKSTTTALIAHILKSAGYDTQLGGNIGTAVLTLDPPK
AGRYYVVECSSYQIDLAPTLNPSAGILLNLTPDHLDRHGTMQHYADVKERLVAGSDVAIVGVDDSHSALIADRVERADVK
VVRISRRSVVASGIYAEGSRLIQAAGGAMLPFADLDGIQTLRGSHNAQNAAAAVAACLAVGVSADDIRAGLASFPGLKHR
MQPVGQRGRVVFVNDSKATNADAAAPALSSYDRIYWIAGGLPKAGGITTLAPYFPRIAKAYLIGEAAAEFAATLGEAVPY
EISGTLERAVVHAAADAERDEAAASAVMLSPACASFDQYKNFEVRGEAFVGHVAALDGIAMLIGSATGEK
>Mature_470_residues
MIPVTTLKDRKVALFGLGGSGLATARALVSGGAEVTAWDDNPDSVAKASAEGIRTEDLHTIDWSQQALFVLSPGVPLTHP
KPHWTVDLARAAGVDIVGDVELFVRERRAHAPDCPFIAITGTNGKSTTTALIAHILKSAGYDTQLGGNIGTAVLTLDPPK
AGRYYVVECSSYQIDLAPTLNPSAGILLNLTPDHLDRHGTMQHYADVKERLVAGSDVAIVGVDDSHSALIADRVERADVK
VVRISRRSVVASGIYAEGSRLIQAAGGAMLPFADLDGIQTLRGSHNAQNAAAAVAACLAVGVSADDIRAGLASFPGLKHR
MQPVGQRGRVVFVNDSKATNADAAAPALSSYDRIYWIAGGLPKAGGITTLAPYFPRIAKAYLIGEAAAEFAATLGEAVPY
EISGTLERAVVHAAADAERDEAAASAVMLSPACASFDQYKNFEVRGEAFVGHVAALDGIAMLIGSATGEK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=448, Percent_Identity=33.0357142857143, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48990; Mature: 48990

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVTTLKDRKVALFGLGGSGLATARALVSGGAEVTAWDDNPDSVAKASAEGIRTEDLHT
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCEEE
IDWSQQALFVLSPGVPLTHPKPHWTVDLARAAGVDIVGDVELFVRERRAHAPDCPFIAIT
ECCCCCEEEEEECCCCCCCCCCCEEEEEHHHCCCCEEECHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHILKSAGYDTQLGGNIGTAVLTLDPPKAGRYYVVECSSYQIDLAPTL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
NPSAGILLNLTPDHLDRHGTMQHYADVKERLVAGSDVAIVGVDDSHSALIADRVERADVK
CCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEE
VVRISRRSVVASGIYAEGSRLIQAAGGAMLPFADLDGIQTLRGSHNAQNAAAAVAACLAV
EEEEECHHHHHCCCHHCCCHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHH
GVSADDIRAGLASFPGLKHRMQPVGQRGRVVFVNDSKATNADAAAPALSSYDRIYWIAGG
CCCHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHCCCEEEEEECC
LPKAGGITTLAPYFPRIAKAYLIGEAAAEFAATLGEAVPYEISGTLERAVVHAAADAERD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCHH
EAAASAVMLSPACASFDQYKNFEVRGEAFVGHVAALDGIAMLIGSATGEK
HHHHHHEEECHHHHCHHHHCCEEECCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIPVTTLKDRKVALFGLGGSGLATARALVSGGAEVTAWDDNPDSVAKASAEGIRTEDLHT
CCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCEEE
IDWSQQALFVLSPGVPLTHPKPHWTVDLARAAGVDIVGDVELFVRERRAHAPDCPFIAIT
ECCCCCEEEEEECCCCCCCCCCCEEEEEHHHCCCCEEECHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHILKSAGYDTQLGGNIGTAVLTLDPPKAGRYYVVECSSYQIDLAPTL
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
NPSAGILLNLTPDHLDRHGTMQHYADVKERLVAGSDVAIVGVDDSHSALIADRVERADVK
CCCCCEEEECCHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEE
VVRISRRSVVASGIYAEGSRLIQAAGGAMLPFADLDGIQTLRGSHNAQNAAAAVAACLAV
EEEEECHHHHHCCCHHCCCHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHH
GVSADDIRAGLASFPGLKHRMQPVGQRGRVVFVNDSKATNADAAAPALSSYDRIYWIAGG
CCCHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHCCCEEEEEECC
LPKAGGITTLAPYFPRIAKAYLIGEAAAEFAATLGEAVPYEISGTLERAVVHAAADAERD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHCCCCHH
EAAASAVMLSPACASFDQYKNFEVRGEAFVGHVAALDGIAMLIGSATGEK
HHHHHHEEECHHHHCHHHHCCEEECCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA