Click here to switch to the map view.

The map label for this gene is nthA [H]

Identifier: 209550109

GI number: 209550109

Start: 2557078

End: 2557773

Strand: Reverse

Name: nthA [H]

Synonym: Rleg2_2526

Alternate gene names: 209550109

Gene position: 2557773-2557078 (Counterclockwise)

Preceding gene: 209550112

Following gene: 209550108

Centisome position: 56.36

GC content: 63.07

Gene sequence:

>696_bases
TTGCACGACGATCACGACCACGACCACCACGATCATGACCACGACCACCACCACGATAACCACTATTCCGACATGCAGGC
CCGCGTAAAAGCGCTGGAAACGCTGCTGACCGAGAAGGGGCTGATCGATCCGGCGGCGATCGATGCGATTGTCGAGACTT
ATGAAACGAAGGTGGGGCCGCGGAATGGGGCGCATGTCGTCGCCAAGGCTTGGAGCGATGCCGATTTCGCCGCGTGGCTG
AAGCGCGATGCGACGGCGGCGATTGCGAGCCTTGGGTTTACCGGTCGGCAGGGCGAGCATATGCGCGCCGTCTTCAACAC
GCCCGAGACCCATAATCTCATCGTCTGCACGCTTTGCTCCTGTTATCCCTGGTCGGTGCTCGGCCTGCCGCCGGTCTGGT
ACAAGGCGCCGGCTTATCGCTCCCGTGCGGTCATCGATCCGCGTGGCGTGCTTGCCGAATTCGGTCTGACGCTGCCGGAG
GAGAAGAAGATCCGCGTCTGGGATTCGACGGCGGAGCTGCGTTATCTCGTTATCCCCGAGCGGCCGGACGGCACGGACGG
AATGGACGAGGCAGCACTTGCCGGCCTCGTCAGCCGCGATGCGATGATCGGCACGGCCATCGCAGGCAGCTGGAAGGTCT
CAGCTGCCGGCAGTTCGAAGATTTCAGCTGCCGGCAGCCCGGAGGCTCTTCCATGA

Upstream 100 bases:

>100_bases
CTCATCCGACCCTTCGGGCCACCTTCTCCCCACTGGGGAGAAGAGGGAGCAGGCCGCGAACCTTGCCTATCCTTTAGACT
GCCGTTTTGGAGGACATGCC

Downstream 100 bases:

>100_bases
ACGGACCGCACGATCTCGGCGGGCAGATGGGCTTCGGGCCCGTCGCGCCCGAAACGGACGAACCCTATTTCCATGCCGAA
TGGGAAAAGCGGGCGCTCGG

Product: nitrile hydratase, subunit alpha

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MHDDHDHDHHDHDHDHHHDNHYSDMQARVKALETLLTEKGLIDPAAIDAIVETYETKVGPRNGAHVVAKAWSDADFAAWL
KRDATAAIASLGFTGRQGEHMRAVFNTPETHNLIVCTLCSCYPWSVLGLPPVWYKAPAYRSRAVIDPRGVLAEFGLTLPE
EKKIRVWDSTAELRYLVIPERPDGTDGMDEAALAGLVSRDAMIGTAIAGSWKVSAAGSSKISAAGSPEALP

Sequences:

>Translated_231_residues
MHDDHDHDHHDHDHDHHHDNHYSDMQARVKALETLLTEKGLIDPAAIDAIVETYETKVGPRNGAHVVAKAWSDADFAAWL
KRDATAAIASLGFTGRQGEHMRAVFNTPETHNLIVCTLCSCYPWSVLGLPPVWYKAPAYRSRAVIDPRGVLAEFGLTLPE
EKKIRVWDSTAELRYLVIPERPDGTDGMDEAALAGLVSRDAMIGTAIAGSWKVSAAGSSKISAAGSPEALP
>Mature_231_residues
MHDDHDHDHHDHDHDHHHDNHYSDMQARVKALETLLTEKGLIDPAAIDAIVETYETKVGPRNGAHVVAKAWSDADFAAWL
KRDATAAIASLGFTGRQGEHMRAVFNTPETHNLIVCTLCSCYPWSVLGLPPVWYKAPAYRSRAVIDPRGVLAEFGLTLPE
EKKIRVWDSTAELRYLVIPERPDGTDGMDEAALAGLVSRDAMIGTAIAGSWKVSAAGSSKISAAGSPEALP

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 25189; Mature: 25189

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDDHDHDHHDHDHDHHHDNHYSDMQARVKALETLLTEKGLIDPAAIDAIVETYETKVGP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
RNGAHVVAKAWSDADFAAWLKRDATAAIASLGFTGRQGEHMRAVFNTPETHNLIVCTLCS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEECC
CYPWSVLGLPPVWYKAPAYRSRAVIDPRGVLAEFGLTLPEEKKIRVWDSTAELRYLVIPE
CCCCHHCCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEC
RPDGTDGMDEAALAGLVSRDAMIGTAIAGSWKVSAAGSSKISAAGSPEALP
CCCCCCCHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCEECCCCCCCCC
>Mature Secondary Structure
MHDDHDHDHHDHDHDHHHDNHYSDMQARVKALETLLTEKGLIDPAAIDAIVETYETKVGP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
RNGAHVVAKAWSDADFAAWLKRDATAAIASLGFTGRQGEHMRAVFNTPETHNLIVCTLCS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEECC
CYPWSVLGLPPVWYKAPAYRSRAVIDPRGVLAEFGLTLPEEKKIRVWDSTAELRYLVIPE
CCCCHHCCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEC
RPDGTDGMDEAALAGLVSRDAMIGTAIAGSWKVSAAGSSKISAAGSPEALP
CCCCCCCHHHHHHHHHHHHHHHHHHEECCCEEEECCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]