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The map label for this gene is 209550108

Identifier: 209550108

GI number: 209550108

Start: 2556422

End: 2557081

Strand: Reverse

Name: 209550108

Synonym: Rleg2_2525

Alternate gene names: NA

Gene position: 2557081-2556422 (Counterclockwise)

Preceding gene: 209550109

Following gene: 209550107

Centisome position: 56.35

GC content: 62.88

Gene sequence:

>660_bases
ATGAACGGACCGCACGATCTCGGCGGGCAGATGGGCTTCGGGCCCGTCGCGCCCGAAACGGACGAACCCTATTTCCATGC
CGAATGGGAAAAGCGGGCGCTCGGCATCACGCTTTCCTGCGGCGCCTTCGGCGCATGGACGATCGATGAAAGCCGGCATG
CGCGCGAAAACATTCCACCGGCCGATTATCTCGCTGCCAGCTATTACGAGATCTGGATCCGCGGCGTCGAGACGTTGCTG
GCGCGGCACGGTTTTGCGACGCGCGAGGAGTTGCTGTCCGGCCGCAAGATGCAGGAAGGTGCTGCGCCGAAACGGGTGCT
GAAGGCGGAGATGGTGCCGGGCGCGTTGGCGAGAGGCGGGCCTTGCGATCGTCCTGTCGAAACCACACCGCGCTTTGCGG
TCGGCGAGACGGTGAGGACGAAGAATTTCAATCCAGCCACCCATACGCGGCTGCCATGTTATGCGCGCCCCAAGACGGGC
GTCGTCGAGACGGTGCAAGGAGCTTTCGTCTTCCCCGACGACAATGCGCATGGCCGAGGCGAAAATCCGCAATGGCTCTA
TACGGTCGTCTTTGGCGGTGGTGAGATCTGGGGCGAGGGAGCGGATCCGTCGCTGACGGTCTCCATCGATGCGTGGGAGA
GCTATCTTGAGCGTTTGTGA

Upstream 100 bases:

>100_bases
GCCGCGATGCGATGATCGGCACGGCCATCGCAGGCAGCTGGAAGGTCTCAGCTGCCGGCAGTTCGAAGATTTCAGCTGCC
GGCAGCCCGGAGGCTCTTCC

Downstream 100 bases:

>100_bases
GACGCCGTCTCAGCTGGCGCAATCGCTTGGGTTGCCGAGGTCGCCCGAAGGTGATCCGGTTTTTCCCGAGCCTTGGGCGG
CGGACGCTTTCGCCATGACG

Product: nitrile hydratase

Products: NA

Alternate protein names: L-NHase; L-nitrilase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNGPHDLGGQMGFGPVAPETDEPYFHAEWEKRALGITLSCGAFGAWTIDESRHARENIPPADYLAASYYEIWIRGVETLL
ARHGFATREELLSGRKMQEGAAPKRVLKAEMVPGALARGGPCDRPVETTPRFAVGETVRTKNFNPATHTRLPCYARPKTG
VVETVQGAFVFPDDNAHGRGENPQWLYTVVFGGGEIWGEGADPSLTVSIDAWESYLERL

Sequences:

>Translated_219_residues
MNGPHDLGGQMGFGPVAPETDEPYFHAEWEKRALGITLSCGAFGAWTIDESRHARENIPPADYLAASYYEIWIRGVETLL
ARHGFATREELLSGRKMQEGAAPKRVLKAEMVPGALARGGPCDRPVETTPRFAVGETVRTKNFNPATHTRLPCYARPKTG
VVETVQGAFVFPDDNAHGRGENPQWLYTVVFGGGEIWGEGADPSLTVSIDAWESYLERL
>Mature_219_residues
MNGPHDLGGQMGFGPVAPETDEPYFHAEWEKRALGITLSCGAFGAWTIDESRHARENIPPADYLAASYYEIWIRGVETLL
ARHGFATREELLSGRKMQEGAAPKRVLKAEMVPGALARGGPCDRPVETTPRFAVGETVRTKNFNPATHTRLPCYARPKTG
VVETVQGAFVFPDDNAHGRGENPQWLYTVVFGGGEIWGEGADPSLTVSIDAWESYLERL

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit beta family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008990
- InterPro:   IPR003168 [H]

Pfam domain/function: NA

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 24058; Mature: 24058

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGPHDLGGQMGFGPVAPETDEPYFHAEWEKRALGITLSCGAFGAWTIDESRHARENIPP
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCEEEEECCCHHHHCCCCC
ADYLAASYYEIWIRGVETLLARHGFATREELLSGRKMQEGAAPKRVLKAEMVPGALARGG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHCCC
PCDRPVETTPRFAVGETVRTKNFNPATHTRLPCYARPKTGVVETVQGAFVFPDDNAHGRG
CCCCCCCCCCCEECCCCEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCC
ENPQWLYTVVFGGGEIWGEGADPSLTVSIDAWESYLERL
CCCCEEEEEEEECCEEECCCCCCEEEEEHHHHHHHHHCC
>Mature Secondary Structure
MNGPHDLGGQMGFGPVAPETDEPYFHAEWEKRALGITLSCGAFGAWTIDESRHARENIPP
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCEEEEECCCHHHHCCCCC
ADYLAASYYEIWIRGVETLLARHGFATREELLSGRKMQEGAAPKRVLKAEMVPGALARGG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHCCC
PCDRPVETTPRFAVGETVRTKNFNPATHTRLPCYARPKTGVVETVQGAFVFPDDNAHGRG
CCCCCCCCCCCEECCCCEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCCCCC
ENPQWLYTVVFGGGEIWGEGADPSLTVSIDAWESYLERL
CCCCEEEEEEEECCEEECCCCCCEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1840499 [H]