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The map label for this gene is ilvI [H]

Identifier: 209549865

GI number: 209549865

Start: 2336491

End: 2338212

Strand: Reverse

Name: ilvI [H]

Synonym: Rleg2_2280

Alternate gene names: 209549865

Gene position: 2338212-2336491 (Counterclockwise)

Preceding gene: 209549866

Following gene: 209549863

Centisome position: 51.53

GC content: 62.6

Gene sequence:

>1722_bases
ATGAGACCCCGTCATTCCATCGACACCCCGCGCGCCCAGATGACCGGCGCCGAAATCGTTCTCCAGGCGCTGAGAGACAA
TGGCGTCAAGCATATATTCGGCTATCCCGGCGCAGCCGTGCTGCCGATCTACGACGAGCTTTTCCAGCAGGAAGACATCA
GGCACATTCTGGTTCGCCATGAACAGGGAGCCGGCCATGCCGCCGAGGGCTATGCCCGCTCGACGGGCAAGGTCGGCGTC
ATGCTGGTGACCTCTGGACCCGGCGTCACCAACGCGATCACGCCGCTTCAGGATGCCTTGATGGATTCCGTGCCGCTCGT
CTGCCTGTCCGGCCAGGTGCCGACGACGCTGATCGGCTCCGATGCGTTTCAGGAATGCGATACCGTCGGCATCACCCGAG
CCTGCACAAAACACAATCGGCTGGTCAACGACGTCAATGAGCTCGCGGCGGTCATCCACGAGGCCTTTCGCATCGCCGCA
TCAGGCCGGCCCGGACCCGTTCTGGTCGACATGCCCAAAGACGTGCTGTTTGCAAGCGGCAGCTATGCACCGCCTGAGGC
TGTCGCCCCGAGACCGAGCTACCAGCCGGACCACAGCTGCGGCATCAGGGAGATCGAGGCTGCAATCGCTCTGATGGCAA
CGGCACGCAGGCCGGTTATCTGCGTGGGCGGCGGCGTCATCAATTCCGGCCCCGAAGCGGTAAATCGGCTGCGCGAGCTG
GTCGATCTCACCGATTTTCCGGTCACGTCGACGCTGATGGGGCTCGGCGCTCATCCGGCTTCCAGCCCGAACTGGCTGAA
GATGGCCGGGCTTCACGGCTCCCATGAGGCCAATATGGCAATGCAGGACTGCGACCTCATGCTCTGCATCGGCGCGCGCT
TCGACGGCGGGCTTGCCGCCGACGTCGATGGTTTTTCACCGAATTCCCGGAAGATTCAGATCGATATCGATGCTGCCTCG
CTCAACAAGACTGTACGCGCCGATATCGGTATCCGGGGCGATGCGGGCGACGTGCTTTCCGAGCTCGTTCGTCTTTGGCG
GGCGCTTCAGCGGGCGCCGGACCGGGCGAGATTGGCCGAGTGGTGGAGCATGATTGCAGGCTGGCGCGCACGGCGATCGT
TTTCCTATGCGATGCGCGAGGACGTCATCATGCCGCAATATGCCATCGAGCGGCTTTACGAACTGACGAAGGATCGGGAT
ACCTACATCACGACGGAGGTCGGCCGGCATCAGATCTGGGCGGCGCAATATTACGGCTTCGAGCAGCCGAACCGCTGGAT
GATATCAGGCGGCCTGGGGACGATGGGATACGGGCTGCCCGCCGCCCTCGGCGTGCAGATCGCCAATCCCGGCAGCCTCG
TCATCGACATAGCCGGCGATGCCTCCGTGCAGATGACCATGAAGGAAATGTCGGCCGCCGTGCAGCACAACGCGCCGATC
AAGATCTTCATGCTCAATAATGGCGATCAGGGAGCGGTTCGGCTGCACGGAAATCCGGATTTCGTGAAACTGGCGGAAGC
CTATGGCGGACACGGCATTCGCTGTGAGAGGCCGGCCGAACTCGACGATGCGATCCTGGAGATGATCGAGGTGAACAAGC
CTGTTCTGTTCGATTGCCGTGTTGTCGAACTCGCCGATCGCCTGCCGACGATTGGGGCGGGTAGGGCAACCGCCGGTGGC
CACCCTCGTCCTTCGAGGCCCCTTCGGGGCACTTCAGGATGA

Upstream 100 bases:

>100_bases
TAAGCAGGCTCCTGACGGGGCCTTTTTTTATGCCCTGAATCCGGGGCGGGCGCCTCGTCGGACTTTCCACACATCGTCAT
ACTGATCAAGGAACGGAACC

Downstream 100 bases:

>100_bases
GGGCGGAGAGAGGGCGCGGCGCACAGCGTCAACCTATCCCAGCGGCACGGCACACCGATCAGCCCTCATCCTGAGGTGCG
CAGGCCAAAAGGCCGAAGCC

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MRPRHSIDTPRAQMTGAEIVLQALRDNGVKHIFGYPGAAVLPIYDELFQQEDIRHILVRHEQGAGHAAEGYARSTGKVGV
MLVTSGPGVTNAITPLQDALMDSVPLVCLSGQVPTTLIGSDAFQECDTVGITRACTKHNRLVNDVNELAAVIHEAFRIAA
SGRPGPVLVDMPKDVLFASGSYAPPEAVAPRPSYQPDHSCGIREIEAAIALMATARRPVICVGGGVINSGPEAVNRLREL
VDLTDFPVTSTLMGLGAHPASSPNWLKMAGLHGSHEANMAMQDCDLMLCIGARFDGGLAADVDGFSPNSRKIQIDIDAAS
LNKTVRADIGIRGDAGDVLSELVRLWRALQRAPDRARLAEWWSMIAGWRARRSFSYAMREDVIMPQYAIERLYELTKDRD
TYITTEVGRHQIWAAQYYGFEQPNRWMISGGLGTMGYGLPAALGVQIANPGSLVIDIAGDASVQMTMKEMSAAVQHNAPI
KIFMLNNGDQGAVRLHGNPDFVKLAEAYGGHGIRCERPAELDDAILEMIEVNKPVLFDCRVVELADRLPTIGAGRATAGG
HPRPSRPLRGTSG

Sequences:

>Translated_573_residues
MRPRHSIDTPRAQMTGAEIVLQALRDNGVKHIFGYPGAAVLPIYDELFQQEDIRHILVRHEQGAGHAAEGYARSTGKVGV
MLVTSGPGVTNAITPLQDALMDSVPLVCLSGQVPTTLIGSDAFQECDTVGITRACTKHNRLVNDVNELAAVIHEAFRIAA
SGRPGPVLVDMPKDVLFASGSYAPPEAVAPRPSYQPDHSCGIREIEAAIALMATARRPVICVGGGVINSGPEAVNRLREL
VDLTDFPVTSTLMGLGAHPASSPNWLKMAGLHGSHEANMAMQDCDLMLCIGARFDGGLAADVDGFSPNSRKIQIDIDAAS
LNKTVRADIGIRGDAGDVLSELVRLWRALQRAPDRARLAEWWSMIAGWRARRSFSYAMREDVIMPQYAIERLYELTKDRD
TYITTEVGRHQIWAAQYYGFEQPNRWMISGGLGTMGYGLPAALGVQIANPGSLVIDIAGDASVQMTMKEMSAAVQHNAPI
KIFMLNNGDQGAVRLHGNPDFVKLAEAYGGHGIRCERPAELDDAILEMIEVNKPVLFDCRVVELADRLPTIGAGRATAGG
HPRPSRPLRGTSG
>Mature_573_residues
MRPRHSIDTPRAQMTGAEIVLQALRDNGVKHIFGYPGAAVLPIYDELFQQEDIRHILVRHEQGAGHAAEGYARSTGKVGV
MLVTSGPGVTNAITPLQDALMDSVPLVCLSGQVPTTLIGSDAFQECDTVGITRACTKHNRLVNDVNELAAVIHEAFRIAA
SGRPGPVLVDMPKDVLFASGSYAPPEAVAPRPSYQPDHSCGIREIEAAIALMATARRPVICVGGGVINSGPEAVNRLREL
VDLTDFPVTSTLMGLGAHPASSPNWLKMAGLHGSHEANMAMQDCDLMLCIGARFDGGLAADVDGFSPNSRKIQIDIDAAS
LNKTVRADIGIRGDAGDVLSELVRLWRALQRAPDRARLAEWWSMIAGWRARRSFSYAMREDVIMPQYAIERLYELTKDRD
TYITTEVGRHQIWAAQYYGFEQPNRWMISGGLGTMGYGLPAALGVQIANPGSLVIDIAGDASVQMTMKEMSAAVQHNAPI
KIFMLNNGDQGAVRLHGNPDFVKLAEAYGGHGIRCERPAELDDAILEMIEVNKPVLFDCRVVELADRLPTIGAGRATAGG
HPRPSRPLRGTSG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=583, Percent_Identity=25.0428816466552, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI21361361, Length=583, Percent_Identity=24.5283018867925, Blast_Score=135, Evalue=9e-32,
Organism=Escherichia coli, GI87081685, Length=548, Percent_Identity=46.5328467153285, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=39.0763765541741, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1786717, Length=577, Percent_Identity=32.2357019064125, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1787096, Length=554, Percent_Identity=27.9783393501805, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788716, Length=562, Percent_Identity=26.1565836298932, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=584, Percent_Identity=25, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=584, Percent_Identity=25, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=513, Percent_Identity=26.1208576998051, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=593, Percent_Identity=41.8212478920742, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320816, Length=511, Percent_Identity=24.853228962818, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922626, Length=579, Percent_Identity=25.73402417962, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61843; Mature: 61843

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRHSIDTPRAQMTGAEIVLQALRDNGVKHIFGYPGAAVLPIYDELFQQEDIRHILVRH
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHCC
EQGAGHAAEGYARSTGKVGVMLVTSGPGVTNAITPLQDALMDSVPLVCLSGQVPTTLIGS
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEECC
DAFQECDTVGITRACTKHNRLVNDVNELAAVIHEAFRIAASGRPGPVLVDMPKDVLFASG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHEEECC
SYAPPEAVAPRPSYQPDHSCGIREIEAAIALMATARRPVICVGGGVINSGPEAVNRLREL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
VDLTDFPVTSTLMGLGAHPASSPNWLKMAGLHGSHEANMAMQDCDLMLCIGARFDGGLAA
HHCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHCCCEEEEEECCCCCCEEE
DVDGFSPNSRKIQIDIDAASLNKTVRADIGIRGDAGDVLSELVRLWRALQRAPDRARLAE
CCCCCCCCCCEEEEEEEHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
WWSMIAGWRARRSFSYAMREDVIMPQYAIERLYELTKDRDTYITTEVGRHQIWAAQYYGF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEHHHCCC
EQPNRWMISGGLGTMGYGLPAALGVQIANPGSLVIDIAGDASVQMTMKEMSAAVQHNAPI
CCCCCEEEECCCCCCCCCCCHHHEEEEECCCCEEEEECCCCCEEHHHHHHHHHHHCCCCE
KIFMLNNGDQGAVRLHGNPDFVKLAEAYGGHGIRCERPAELDDAILEMIEVNKPVLFDCR
EEEEEECCCCCEEEECCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEH
VVELADRLPTIGAGRATAGGHPRPSRPLRGTSG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPRHSIDTPRAQMTGAEIVLQALRDNGVKHIFGYPGAAVLPIYDELFQQEDIRHILVRH
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHCC
EQGAGHAAEGYARSTGKVGVMLVTSGPGVTNAITPLQDALMDSVPLVCLSGQVPTTLIGS
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEECC
DAFQECDTVGITRACTKHNRLVNDVNELAAVIHEAFRIAASGRPGPVLVDMPKDVLFASG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHEEECC
SYAPPEAVAPRPSYQPDHSCGIREIEAAIALMATARRPVICVGGGVINSGPEAVNRLREL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHH
VDLTDFPVTSTLMGLGAHPASSPNWLKMAGLHGSHEANMAMQDCDLMLCIGARFDGGLAA
HHCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCHHHCCCEEEEEECCCCCCEEE
DVDGFSPNSRKIQIDIDAASLNKTVRADIGIRGDAGDVLSELVRLWRALQRAPDRARLAE
CCCCCCCCCCEEEEEEEHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
WWSMIAGWRARRSFSYAMREDVIMPQYAIERLYELTKDRDTYITTEVGRHQIWAAQYYGF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEHHHCCC
EQPNRWMISGGLGTMGYGLPAALGVQIANPGSLVIDIAGDASVQMTMKEMSAAVQHNAPI
CCCCCEEEECCCCCCCCCCCHHHEEEEECCCCEEEEECCCCCEEHHHHHHHHHHHCCCCE
KIFMLNNGDQGAVRLHGNPDFVKLAEAYGGHGIRCERPAELDDAILEMIEVNKPVLFDCR
EEEEEECCCCCEEEECCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEH
VVELADRLPTIGAGRATAGGHPRPSRPLRGTSG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10555290 [H]