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The map label for this gene is cusS [H]

Identifier: 209549805

GI number: 209549805

Start: 2274571

End: 2275914

Strand: Reverse

Name: cusS [H]

Synonym: Rleg2_2216

Alternate gene names: 209549805

Gene position: 2275914-2274571 (Counterclockwise)

Preceding gene: 209549806

Following gene: 209549804

Centisome position: 50.15

GC content: 65.4

Gene sequence:

>1344_bases
ATGGGCGAAAAACGCAAGCCTAACCCTTCGCTCTGGTGGCGGCTCAGCTGGCAGCTCAGCCTGGTCTTCGTCGGCGTGGT
GACGGCTGTCACCATCGGGCTCTGCTTCTACGGCGCGACGATCCTCTCACCGAACGTCGCGCTGGAGGACCATGTCACCG
CCGCATTGGCTCCGGCCGTTGCGCTCAATGCCGAGGGACGGCTCGAAATAAAGGATACGCCGGAGCTGGATGCACTCAAG
CGGCAGTATGACGGGCTTTGGTATGTCGCGGCCACGACGAACGGTTCTTTCGTATCTTACGGGACCATTCCTGCAGCCTA
CAGCGAGCTTGCGCGTCTGACCTACCTGTTCGAAGGGGCCGATATCAGGGGATCGGCCGGCACGGGCGAGATCGCCTCGG
TCGAAAGCGTGGAGACCGCGATCGGCGAGCTACGGGTTCTGTTTGGCGGCGTTGCCGACAAGGGCTGGCCGGTTCTTACC
CTGATGGGCGCCGTTTATCCGATCTACGTCCCGCTGTTCGTCATCGCCTTGCCCGCGGTTTTTCTCGCCGTTCCCAGGAT
CGTCCGGCGTGGGCTGGCCAGCGTCAGCGAGGTCGCCCGCAAGGCATCCGAGATCGACGCCCGCCGCTACGATGCGCGCC
TGCCGCTCGACGGCATTCCGCGGGAGGTCGCGCCGCTGGTGATCGCCTTCAACGAGGCGCTCGACAGGCTGGAAAACGAG
TACCGCAAACGGCAACACTTCCTGATCGATGCGGCGCATGAACTGAGGACGCCGATCGCGATCATGCAGACCCGGATCGA
TGGAACGGCCGAAGGACAGGACCGCCGGCGATTGCTCGACGACGTCGCGCGTTTGGCCGAGACGGCCGAGCAGCTGCTCG
ATTTCGAGCGCAACGACCAGGCGACCGATCTCCACGAAATGGTCGATCTCGTCGAGATTGCCCGCTCCGTCGTTGCCGAC
CTCGCGCCGCTGGCGATCGGCGCTGGCTACCAGATCTCGTTTCAGAGCGAGGTCGAGAGCCTGAAGCGCAAGGGCAGCCC
CTCGGCGCTGCCGCGGGCCGTGAGCAACCTCGTTCGCAATGCCATCGACCATGGCGGCGGGAGCGGCATGATCCCCGTCT
CCGTGTCGGCCTTCGGTGGCGGTCGGATCAGCGTCGCCGATGAGGGGCCGGGCATTCCGGCCGAGCACCGCGAGCTGGTG
TTCGAACCGTTCTATCGCGTCACTCCGAAGAGCACGGGGGCGGGGCTCGGCCTCAGCCTCGTCAAGCAGATCGCCGCAAA
CCATGGCGGCGAAGTCAGCATCGAGAGCGGGGACGCCGGCACCGAGGTGGCGATCCGGCTGTGA

Upstream 100 bases:

>100_bases
ATGCGCACATTTCGCGACTGCGCAGGCGGCTTGCCGAGGCCGGCGCCGAGGTTTCGATCCACAATATCCGCGGCGTCGGC
TATCTCCTGAAGGAAGAATG

Downstream 100 bases:

>100_bases
TGACGCGGGAAAGGATGTAATGCTGCGGCAATTCTCGACCGCCACTTTTGCCTGAGATCATCGGCGATGGAGGCCAGACA
GAGTGCGCAGCAATCCATTC

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MGEKRKPNPSLWWRLSWQLSLVFVGVVTAVTIGLCFYGATILSPNVALEDHVTAALAPAVALNAEGRLEIKDTPELDALK
RQYDGLWYVAATTNGSFVSYGTIPAAYSELARLTYLFEGADIRGSAGTGEIASVESVETAIGELRVLFGGVADKGWPVLT
LMGAVYPIYVPLFVIALPAVFLAVPRIVRRGLASVSEVARKASEIDARRYDARLPLDGIPREVAPLVIAFNEALDRLENE
YRKRQHFLIDAAHELRTPIAIMQTRIDGTAEGQDRRRLLDDVARLAETAEQLLDFERNDQATDLHEMVDLVEIARSVVAD
LAPLAIGAGYQISFQSEVESLKRKGSPSALPRAVSNLVRNAIDHGGGSGMIPVSVSAFGGGRISVADEGPGIPAEHRELV
FEPFYRVTPKSTGAGLGLSLVKQIAANHGGEVSIESGDAGTEVAIRL

Sequences:

>Translated_447_residues
MGEKRKPNPSLWWRLSWQLSLVFVGVVTAVTIGLCFYGATILSPNVALEDHVTAALAPAVALNAEGRLEIKDTPELDALK
RQYDGLWYVAATTNGSFVSYGTIPAAYSELARLTYLFEGADIRGSAGTGEIASVESVETAIGELRVLFGGVADKGWPVLT
LMGAVYPIYVPLFVIALPAVFLAVPRIVRRGLASVSEVARKASEIDARRYDARLPLDGIPREVAPLVIAFNEALDRLENE
YRKRQHFLIDAAHELRTPIAIMQTRIDGTAEGQDRRRLLDDVARLAETAEQLLDFERNDQATDLHEMVDLVEIARSVVAD
LAPLAIGAGYQISFQSEVESLKRKGSPSALPRAVSNLVRNAIDHGGGSGMIPVSVSAFGGGRISVADEGPGIPAEHRELV
FEPFYRVTPKSTGAGLGLSLVKQIAANHGGEVSIESGDAGTEVAIRL
>Mature_446_residues
GEKRKPNPSLWWRLSWQLSLVFVGVVTAVTIGLCFYGATILSPNVALEDHVTAALAPAVALNAEGRLEIKDTPELDALKR
QYDGLWYVAATTNGSFVSYGTIPAAYSELARLTYLFEGADIRGSAGTGEIASVESVETAIGELRVLFGGVADKGWPVLTL
MGAVYPIYVPLFVIALPAVFLAVPRIVRRGLASVSEVARKASEIDARRYDARLPLDGIPREVAPLVIAFNEALDRLENEY
RKRQHFLIDAAHELRTPIAIMQTRIDGTAEGQDRRRLLDDVARLAETAEQLLDFERNDQATDLHEMVDLVEIARSVVADL
APLAIGAGYQISFQSEVESLKRKGSPSALPRAVSNLVRNAIDHGGGSGMIPVSVSAFGGGRISVADEGPGIPAEHRELVF
EPFYRVTPKSTGAGLGLSLVKQIAANHGGEVSIESGDAGTEVAIRL

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=291, Percent_Identity=30.5841924398625, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1789403, Length=281, Percent_Identity=29.8932384341637, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790346, Length=237, Percent_Identity=30.8016877637131, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786600, Length=209, Percent_Identity=32.0574162679426, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787894, Length=224, Percent_Identity=29.4642857142857, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789808, Length=289, Percent_Identity=27.681660899654, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87082128, Length=245, Percent_Identity=30.2040816326531, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788279, Length=285, Percent_Identity=28.7719298245614, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790551, Length=296, Percent_Identity=26.3513513513513, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=28.5067873303167, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788393, Length=232, Percent_Identity=24.1379310344828, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=230, Percent_Identity=26.5217391304348, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48089; Mature: 47958

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKRKPNPSLWWRLSWQLSLVFVGVVTAVTIGLCFYGATILSPNVALEDHVTAALAPAV
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALNAEGRLEIKDTPELDALKRQYDGLWYVAATTNGSFVSYGTIPAAYSELARLTYLFEGA
EECCCCEEEECCCCCHHHHHHHHCCEEEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCC
DIRGSAGTGEIASVESVETAIGELRVLFGGVADKGWPVLTLMGAVYPIYVPLFVIALPAV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLAVPRIVRRGLASVSEVARKASEIDARRYDARLPLDGIPREVAPLVIAFNEALDRLENE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YRKRQHFLIDAAHELRTPIAIMQTRIDGTAEGQDRRRLLDDVARLAETAEQLLDFERNDQ
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ATDLHEMVDLVEIARSVVADLAPLAIGAGYQISFQSEVESLKRKGSPSALPRAVSNLVRN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHH
AIDHGGGSGMIPVSVSAFGGGRISVADEGPGIPAEHRELVFEPFYRVTPKSTGAGLGLSL
HHHCCCCCCEEEEEEEECCCCEEEECCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCHHHH
VKQIAANHGGEVSIESGDAGTEVAIRL
HHHHHHCCCCEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
GEKRKPNPSLWWRLSWQLSLVFVGVVTAVTIGLCFYGATILSPNVALEDHVTAALAPAV
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALNAEGRLEIKDTPELDALKRQYDGLWYVAATTNGSFVSYGTIPAAYSELARLTYLFEGA
EECCCCEEEECCCCCHHHHHHHHCCEEEEEEECCCCEEEECCCHHHHHHHHHHHHHCCCC
DIRGSAGTGEIASVESVETAIGELRVLFGGVADKGWPVLTLMGAVYPIYVPLFVIALPAV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FLAVPRIVRRGLASVSEVARKASEIDARRYDARLPLDGIPREVAPLVIAFNEALDRLENE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YRKRQHFLIDAAHELRTPIAIMQTRIDGTAEGQDRRRLLDDVARLAETAEQLLDFERNDQ
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ATDLHEMVDLVEIARSVVADLAPLAIGAGYQISFQSEVESLKRKGSPSALPRAVSNLVRN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHH
AIDHGGGSGMIPVSVSAFGGGRISVADEGPGIPAEHRELVFEPFYRVTPKSTGAGLGLSL
HHHCCCCCCEEEEEEEECCCCEEEECCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCHHHH
VKQIAANHGGEVSIESGDAGTEVAIRL
HHHHHHCCCCEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]