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The map label for this gene is dusA [H]

Identifier: 209549798

GI number: 209549798

Start: 2268760

End: 2269761

Strand: Direct

Name: dusA [H]

Synonym: Rleg2_2209

Alternate gene names: 209549798

Gene position: 2268760-2269761 (Clockwise)

Preceding gene: 209549797

Following gene: 209549800

Centisome position: 50.0

GC content: 65.07

Gene sequence:

>1002_bases
ATGACGCAGAAGAACCCGATTTTCGCGGTCGCCCCGATGATCGACTGGACGGATCGGCATTGCCGTTATTTCCATCGCCA
GATCAGCCGGCAGGCGCTGCTCTATACCGAGATGGTGGTGGCCGATGCGATCATTCACGGCCCGCGCGAGCGGCTGCTCG
GCCATGACGCCGCGGAACATCCGCTGGCGCTGCAGCTCGGCGGGTCGGACCCGTCCAAGCTTGCCGAGGCGGTGAAGATA
TCGGAACCCTACGGTTATGACGAAATCAACCTGAATGTCGGCTGCCCCTCCGACCGGGTGCAGTCGGGCACCTTCGGCGC
CTGCCTGATGCTGACGCCGGAAACGGTGGCCGATTGCATCGCGGCGATGAAGAAGGTTGCAACCGTGCCGGTGACGGTGA
AATGCCGGATCGGGGTCGATGAGCAGGAACCGGAACAGGCCTTGCCGGAGCTCATCACTCGCGTTCTCGATGCCGGTGCC
GACGCGATCTGGATCCATGCCCGCAAGGCCTGGCTGAAGGGCCTGAGCCCCAAGGAAAATCGCGAGATCCCGCCGCTCGA
CTATGAGATCGTCTACCGGATGAAACAGCGCTGGCCCGATGTCTTCATCGGCATCAACGGCGGCATCCGCACGCTTGATG
AAGCCGCCGCCCATCTCGCCCATGTCGACGGCGTCATGCTTGGCCGCGCCGCCTATCAGAATGCGGCGATCCTTGCCGAA
GTCGACCAGCGGTTCTTCGGCGCTCCGGCGGGAGAGCCGGACTGGGAAGGCCTGCGCGACCGGATGATGGCCTATGCCGA
ACGCCATATCGCCAGTGGCGGACGGCTGCAGCACGTGGCCCGCCACATGGTCGGCCTGTTCACCGGCCTGCCCGGCGCCA
GGCGCTACCGCCAGATCCTGTCGACCGACGCGGCCAAAAGCGGTGCCGGGCCGGAAGTATTGGCGGCAGCCTTTGCGGCT
GTGGATTTTTCGGGGACGGAGCAGCAGGCGGTCAGCGCCTGA

Upstream 100 bases:

>100_bases
CGCACTGCCAGATGTTATATGCGCGCTCAAGAGATGATCCCGCCCCGTGGCGGTGCGGCGCCCATGCGGCCCCCAGAGAA
CAACGAATTATGGAACGTCG

Downstream 100 bases:

>100_bases
CGGGACGTGACGTCTTTGTCAGGACGTCCGCGCCATCGGCAGCAAGGTCTTCTTTTCGATCCGACCCGCCACCGAGAACA
CCAGTATCTCCGTATCCCTG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTQKNPIFAVAPMIDWTDRHCRYFHRQISRQALLYTEMVVADAIIHGPRERLLGHDAAEHPLALQLGGSDPSKLAEAVKI
SEPYGYDEINLNVGCPSDRVQSGTFGACLMLTPETVADCIAAMKKVATVPVTVKCRIGVDEQEPEQALPELITRVLDAGA
DAIWIHARKAWLKGLSPKENREIPPLDYEIVYRMKQRWPDVFIGINGGIRTLDEAAAHLAHVDGVMLGRAAYQNAAILAE
VDQRFFGAPAGEPDWEGLRDRMMAYAERHIASGGRLQHVARHMVGLFTGLPGARRYRQILSTDAAKSGAGPEVLAAAFAA
VDFSGTEQQAVSA

Sequences:

>Translated_333_residues
MTQKNPIFAVAPMIDWTDRHCRYFHRQISRQALLYTEMVVADAIIHGPRERLLGHDAAEHPLALQLGGSDPSKLAEAVKI
SEPYGYDEINLNVGCPSDRVQSGTFGACLMLTPETVADCIAAMKKVATVPVTVKCRIGVDEQEPEQALPELITRVLDAGA
DAIWIHARKAWLKGLSPKENREIPPLDYEIVYRMKQRWPDVFIGINGGIRTLDEAAAHLAHVDGVMLGRAAYQNAAILAE
VDQRFFGAPAGEPDWEGLRDRMMAYAERHIASGGRLQHVARHMVGLFTGLPGARRYRQILSTDAAKSGAGPEVLAAAFAA
VDFSGTEQQAVSA
>Mature_332_residues
TQKNPIFAVAPMIDWTDRHCRYFHRQISRQALLYTEMVVADAIIHGPRERLLGHDAAEHPLALQLGGSDPSKLAEAVKIS
EPYGYDEINLNVGCPSDRVQSGTFGACLMLTPETVADCIAAMKKVATVPVTVKCRIGVDEQEPEQALPELITRVLDAGAD
AIWIHARKAWLKGLSPKENREIPPLDYEIVYRMKQRWPDVFIGINGGIRTLDEAAAHLAHVDGVMLGRAAYQNAAILAEV
DQRFFGAPAGEPDWEGLRDRMMAYAERHIASGGRLQHVARHMVGLFTGLPGARRYRQILSTDAAKSGAGPEVLAAAFAAV
DFSGTEQQAVSA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=28.8135593220339, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI145693211, Length=315, Percent_Identity=56.5079365079365, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1789660, Length=245, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=26.9461077844311, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=242, Percent_Identity=27.6859504132231, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24580595, Length=246, Percent_Identity=25.609756097561, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI19920448, Length=246, Percent_Identity=25.609756097561, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36413; Mature: 36282

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKNPIFAVAPMIDWTDRHCRYFHRQISRQALLYTEMVVADAIIHGPRERLLGHDAAEH
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
PLALQLGGSDPSKLAEAVKISEPYGYDEINLNVGCPSDRVQSGTFGACLMLTPETVADCI
CEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCCCCEEEEECHHHHHHHH
AAMKKVATVPVTVKCRIGVDEQEPEQALPELITRVLDAGADAIWIHARKAWLKGLSPKEN
HHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCC
REIPPLDYEIVYRMKQRWPDVFIGINGGIRTLDEAAAHLAHVDGVMLGRAAYQNAAILAE
CCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VDQRFFGAPAGEPDWEGLRDRMMAYAERHIASGGRLQHVARHMVGLFTGLPGARRYRQIL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
STDAAKSGAGPEVLAAAFAAVDFSGTEQQAVSA
HHHHHCCCCCHHHHHHHHHHHCCCCCHHHHCCC
>Mature Secondary Structure 
TQKNPIFAVAPMIDWTDRHCRYFHRQISRQALLYTEMVVADAIIHGPRERLLGHDAAEH
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
PLALQLGGSDPSKLAEAVKISEPYGYDEINLNVGCPSDRVQSGTFGACLMLTPETVADCI
CEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHCCCCCCCEEEEECHHHHHHHH
AAMKKVATVPVTVKCRIGVDEQEPEQALPELITRVLDAGADAIWIHARKAWLKGLSPKEN
HHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCC
REIPPLDYEIVYRMKQRWPDVFIGINGGIRTLDEAAAHLAHVDGVMLGRAAYQNAAILAE
CCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VDQRFFGAPAGEPDWEGLRDRMMAYAERHIASGGRLQHVARHMVGLFTGLPGARRYRQIL
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
STDAAKSGAGPEVLAAAFAAVDFSGTEQQAVSA
HHHHHCCCCCHHHHHHHHHHHCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]