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The map label for this gene is hsrA [H]

Identifier: 209549758

GI number: 209549758

Start: 2221725

End: 2223170

Strand: Reverse

Name: hsrA [H]

Synonym: Rleg2_2166

Alternate gene names: 209549758

Gene position: 2223170-2221725 (Counterclockwise)

Preceding gene: 209549760

Following gene: 209549757

Centisome position: 48.99

GC content: 63.69

Gene sequence:

>1446_bases
ATGACCACGGCAGAGACGAGCGAGAATGGGCAGGAGGCGGCAAGCGGCCGCCAGGCGAAGATCGTGGCGCTGGTCGTTGC
CGTTTCCTTCTTCATGCAGATCCTCGACGGCACGATCGTCGCCACCTCGCTGCCGCAGATGGCGGCGAGCTTTGACGTGC
AGCCGGTGTCGATGAGCATCGGCATTACCGTCTACATGCTGACGATGGCCGCTTTCATTCCGCTCTCGGGCTGGCTCGGC
GACAGGTTCGGTGCGCGCCGTATCTTCCTGATGTCGATCGCCGTCTTTACCGCCGCCTCGCTATTCTGCGGCCTGTCCGG
CAGCCTCACGGAATTTGTGCTTTGGCGCGCCGTCCAAGGCGCGGGCAGCGCGCTGATGACGCCGGTCGGGCGGATCATCG
TGCTGAAGAACGCCCGCAAATCCGAACTCGTCCAGGCGATCGCGCTGATCACCTGGCCGGCGCTGACCGCACCGGTGATC
GGCCCGGTGCTCGGCGGCTTCATCACGACCTATGCCAGCTGGCACTGGAACTTCCTCATAAACATCCCGATCGGCATTCT
CGGCATGGCGCTGGTGCTGCGCTTCGTGCCGGAGCAGCGCGAGACAGATCCCGGCCGGTTGGATTTCGCCGGCTTCGTTC
TGAGTGCTGCGGGGCTGACCTTCCTGCTCGCCGCCCTGGAGCTTTCGGTCAAATGGGACGGCGGGCTTTTGCCGGTCATA
TCCATGCTTGCAGCCGGCATCGTGCTGTCTGTGATGGCGACCCGGCATTTCCTCGCTGTCGACAATCCGCTGCTCGACCT
TTCGGCCTTCCGCGTCCAGACCTTCTCGATGTCGACGCTGTCGGCGGGCACGGCCTGCCGGGTGGCGATCAATGCGACGC
CGTTCCTGCTGCCGCTGCTGTTCCAGCTCGGTTTCGGGCTGAGCTCGATTGCCGCCGGCACCTATCTGCTAGTCTACTTC
CTCGGCAATCTCGGCATGAAGACGGTGACGACGCCGCTCTTGCGCTTCTTCGGCTTCCGCATCGTGCTTGTGGTCAACGG
GCTGATTGCGGCGCTTTCAATCGCCGCCTGCGGTTTCCTCACGCCGGATACGCCGCAGTTTTTCATCCATGCGCTGCTTT
TTCTCGCCGGCCTGTCGCGGTCGATGGAATTCACCGCGCTGAACACACTCGCCTTCGCCGATATCGGTCCGGCGCAGCGA
AGCTCGGCCTCGACGCTGTCGAGCATGCTGCAGCAGGTGTCGATGCTGCTCGGCGTCGCCGTTGCGGCGGCCGTGCTGAA
TATCGCTTCGGCTCTCAGGGGCGCCGACAATCCCGTTCTTGCCGATTTCCGCTGGGCTTTCGTCGTGGTCGGCGCGATCG
GCGTCATGTCGTCGCTGCGCTTCCTGCAATTGCCGGCGGAGGCAGGCGCCGAAGTATCCGGCCATCGGAAATTCCAGAAA
AATTAG

Upstream 100 bases:

>100_bases
AGTAGGCGGTTCATATCCGCATATCCCCGCGCGGCGGATTTCCAGTCAGCTTCCTTCCCGTTTCCCTGCCGCGGTATCCT
GAATTTTCATTGGCGGATCG

Downstream 100 bases:

>100_bases
CCCGCGGCGGAACCAATCGGGCCGCTCCAATCTTCTTCAACGGCTTCCATCGGATCCTCCTCCAGGCCAGGTCGTGTTCT
GAAAATGCCGGCTTCCCGCC

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTTAETSENGQEAASGRQAKIVALVVAVSFFMQILDGTIVATSLPQMAASFDVQPVSMSIGITVYMLTMAAFIPLSGWLG
DRFGARRIFLMSIAVFTAASLFCGLSGSLTEFVLWRAVQGAGSALMTPVGRIIVLKNARKSELVQAIALITWPALTAPVI
GPVLGGFITTYASWHWNFLINIPIGILGMALVLRFVPEQRETDPGRLDFAGFVLSAAGLTFLLAALELSVKWDGGLLPVI
SMLAAGIVLSVMATRHFLAVDNPLLDLSAFRVQTFSMSTLSAGTACRVAINATPFLLPLLFQLGFGLSSIAAGTYLLVYF
LGNLGMKTVTTPLLRFFGFRIVLVVNGLIAALSIAACGFLTPDTPQFFIHALLFLAGLSRSMEFTALNTLAFADIGPAQR
SSASTLSSMLQQVSMLLGVAVAAAVLNIASALRGADNPVLADFRWAFVVVGAIGVMSSLRFLQLPAEAGAEVSGHRKFQK
N

Sequences:

>Translated_481_residues
MTTAETSENGQEAASGRQAKIVALVVAVSFFMQILDGTIVATSLPQMAASFDVQPVSMSIGITVYMLTMAAFIPLSGWLG
DRFGARRIFLMSIAVFTAASLFCGLSGSLTEFVLWRAVQGAGSALMTPVGRIIVLKNARKSELVQAIALITWPALTAPVI
GPVLGGFITTYASWHWNFLINIPIGILGMALVLRFVPEQRETDPGRLDFAGFVLSAAGLTFLLAALELSVKWDGGLLPVI
SMLAAGIVLSVMATRHFLAVDNPLLDLSAFRVQTFSMSTLSAGTACRVAINATPFLLPLLFQLGFGLSSIAAGTYLLVYF
LGNLGMKTVTTPLLRFFGFRIVLVVNGLIAALSIAACGFLTPDTPQFFIHALLFLAGLSRSMEFTALNTLAFADIGPAQR
SSASTLSSMLQQVSMLLGVAVAAAVLNIASALRGADNPVLADFRWAFVVVGAIGVMSSLRFLQLPAEAGAEVSGHRKFQK
N
>Mature_480_residues
TTAETSENGQEAASGRQAKIVALVVAVSFFMQILDGTIVATSLPQMAASFDVQPVSMSIGITVYMLTMAAFIPLSGWLGD
RFGARRIFLMSIAVFTAASLFCGLSGSLTEFVLWRAVQGAGSALMTPVGRIIVLKNARKSELVQAIALITWPALTAPVIG
PVLGGFITTYASWHWNFLINIPIGILGMALVLRFVPEQRETDPGRLDFAGFVLSAAGLTFLLAALELSVKWDGGLLPVIS
MLAAGIVLSVMATRHFLAVDNPLLDLSAFRVQTFSMSTLSAGTACRVAINATPFLLPLLFQLGFGLSSIAAGTYLLVYFL
GNLGMKTVTTPLLRFFGFRIVLVVNGLIAALSIAACGFLTPDTPQFFIHALLFLAGLSRSMEFTALNTLAFADIGPAQRS
SASTLSSMLQQVSMLLGVAVAAAVLNIASALRGADNPVLADFRWAFVVVGAIGVMSSLRFLQLPAEAGAEVSGHRKFQKN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=458, Percent_Identity=36.6812227074236, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1788392, Length=460, Percent_Identity=36.7391304347826, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1789042, Length=442, Percent_Identity=26.0180995475113, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1788710, Length=416, Percent_Identity=27.8846153846154, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87081983, Length=342, Percent_Identity=29.8245614035088, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1790146, Length=165, Percent_Identity=28.4848484848485, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=253, Percent_Identity=28.0632411067194, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=284, Percent_Identity=25.3521126760563, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=394, Percent_Identity=24.8730964467005, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50953; Mature: 50822

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAETSENGQEAASGRQAKIVALVVAVSFFMQILDGTIVATSLPQMAASFDVQPVSMSI
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHCCCCEEEEHH
GITVYMLTMAAFIPLSGWLGDRFGARRIFLMSIAVFTAASLFCGLSGSLTEFVLWRAVQG
HHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
AGSALMTPVGRIIVLKNARKSELVQAIALITWPALTAPVIGPVLGGFITTYASWHWNFLI
CCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
NIPIGILGMALVLRFVPEQRETDPGRLDFAGFVLSAAGLTFLLAALELSVKWDGGLLPVI
ECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHH
SMLAAGIVLSVMATRHFLAVDNPLLDLSAFRVQTFSMSTLSAGTACRVAINATPFLLPLL
HHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHEEEECHHHCCCCEEEEEECCCHHHHHHH
FQLGFGLSSIAAGTYLLVYFLGNLGMKTVTTPLLRFFGFRIVLVVNGLIAALSIAACGFL
HHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPDTPQFFIHALLFLAGLSRSMEFTALNTLAFADIGPAQRSSASTLSSMLQQVSMLLGVA
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VAAAVLNIASALRGADNPVLADFRWAFVVVGAIGVMSSLRFLQLPAEAGAEVSGHRKFQK
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCC
N
C
>Mature Secondary Structure 
TTAETSENGQEAASGRQAKIVALVVAVSFFMQILDGTIVATSLPQMAASFDVQPVSMSI
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHCCCCEEEEHH
GITVYMLTMAAFIPLSGWLGDRFGARRIFLMSIAVFTAASLFCGLSGSLTEFVLWRAVQG
HHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
AGSALMTPVGRIIVLKNARKSELVQAIALITWPALTAPVIGPVLGGFITTYASWHWNFLI
CCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
NIPIGILGMALVLRFVPEQRETDPGRLDFAGFVLSAAGLTFLLAALELSVKWDGGLLPVI
ECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHH
SMLAAGIVLSVMATRHFLAVDNPLLDLSAFRVQTFSMSTLSAGTACRVAINATPFLLPLL
HHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHEEEECHHHCCCCEEEEEECCCHHHHHHH
FQLGFGLSSIAAGTYLLVYFLGNLGMKTVTTPLLRFFGFRIVLVVNGLIAALSIAACGFL
HHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TPDTPQFFIHALLFLAGLSRSMEFTALNTLAFADIGPAQRSSASTLSSMLQQVSMLLGVA
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VAAAVLNIASALRGADNPVLADFRWAFVVVGAIGVMSSLRFLQLPAEAGAEVSGHRKFQK
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]