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The map label for this gene is pdtaS [H]

Identifier: 209549757

GI number: 209549757

Start: 2220407

End: 2221483

Strand: Reverse

Name: pdtaS [H]

Synonym: Rleg2_2165

Alternate gene names: 209549757

Gene position: 2221483-2220407 (Counterclockwise)

Preceding gene: 209549758

Following gene: 209549756

Centisome position: 48.95

GC content: 62.95

Gene sequence:

>1077_bases
ATGAGCACTTCTAGCGGCGATGCGGATGCCGTGATCCACATCCTCTATATCGATGACGACGAGGGGCTCGCCCTCTTGAT
GCAAAAGAACCTTGGCCGGCGCGGCTTTTCGGTCGAATGGGCCGAGAACGGTGCTGCCGGCCTTGCCAGACTCGGCAAAG
GCGGCTTCGACGCCGTCGTGCTCGATCATGTCCTGGCTGGCGAGACCGGTCTCGATATCCTGCCCTGCATCACGGCGCTG
CCGGATCATCCGCCGGTCATCTATGCGACCGGTTCGGACGATACCAGCATCGCGGTCGCAGCGCTGAAAGCCGGCGCCGA
CGACTACATGTTCAAGGGGATCTCGGCCGATTATTTCGACCTGCTCGCCGCCGCCCTCGAACAGGCGCTGGAGCGGGCGC
GCTTTCGCCGCGAGACGGCGCAGGCGCAGGAGGTGATCCGCCAGCAGCGCGACCTTGCCGAGATGCTGCTTGCCGAGGTC
AATCACCGCATCGCCAACAGCCTCGGCCTTGTCGGCGCGCTGATCAGGATGCAATCCTCGATGACCAAGGACCAGGTGGC
GATCGACGCGCTGCACGAAACCCAGATGCGCATCAACGCGATCGCCAGCGTGCACCGCCGGCTCTATACCAACCGGCAGG
TCGGCTCGGTGCAGGTGGATGAATATCTGAACAGCCTGCTTACCGAACTCGAAGCCTCGATGCGCGACGACAAGCGGCCG
CACCGCGTCGTGCTCACGGCTGAGCCCGTCAATCTGGCGACCGACAAGGTGATCACGCTCGGCTTGATCGTCAGCGAACT
CGTGACCAACGCTTTCAAATACGCCTATCCGGACAGTGTGCCGGGCGAAATCCGCGTCTTTGTCGATCAGACGGACGAAG
CGCTGAGGGTCATTGTCGAAGACGATGGGGCGGGGTTCGATCCGGAGGGCCCGGCGAGGGGAACGGGGCTCGGGACGCGG
ATATTGACGGCCATGGCTGCGAGCTTGAAATCGGATTTTGCCTATGATCCCGGGCATGATGGGACGAAGGCGACGCTGGT
GTTTTCGTTGAACGAGGGGAAGCAGGGGGCGGTGTAG

Upstream 100 bases:

>100_bases
CGCTGTTTACCGCAGGCGGCCGATGCAATACGACATTGCCATGCGGACGACTCGGACGCTTGCGGGCGGTCGTCGCTGCG
AGAGAAAGCCGGGAGACGGA

Downstream 100 bases:

>100_bases
GGTGTGCCGTACGGCCCCCTCATCCGCCCTACGGGCACCTTCTCCCCGAGGGGAGAAGAGATATGCCGCGACGCCTCGAT
TCTCTCTTCTCCCCTTGGGG

Product: signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSTSSGDADAVIHILYIDDDEGLALLMQKNLGRRGFSVEWAENGAAGLARLGKGGFDAVVLDHVLAGETGLDILPCITAL
PDHPPVIYATGSDDTSIAVAALKAGADDYMFKGISADYFDLLAAALEQALERARFRRETAQAQEVIRQQRDLAEMLLAEV
NHRIANSLGLVGALIRMQSSMTKDQVAIDALHETQMRINAIASVHRRLYTNRQVGSVQVDEYLNSLLTELEASMRDDKRP
HRVVLTAEPVNLATDKVITLGLIVSELVTNAFKYAYPDSVPGEIRVFVDQTDEALRVIVEDDGAGFDPEGPARGTGLGTR
ILTAMAASLKSDFAYDPGHDGTKATLVFSLNEGKQGAV

Sequences:

>Translated_358_residues
MSTSSGDADAVIHILYIDDDEGLALLMQKNLGRRGFSVEWAENGAAGLARLGKGGFDAVVLDHVLAGETGLDILPCITAL
PDHPPVIYATGSDDTSIAVAALKAGADDYMFKGISADYFDLLAAALEQALERARFRRETAQAQEVIRQQRDLAEMLLAEV
NHRIANSLGLVGALIRMQSSMTKDQVAIDALHETQMRINAIASVHRRLYTNRQVGSVQVDEYLNSLLTELEASMRDDKRP
HRVVLTAEPVNLATDKVITLGLIVSELVTNAFKYAYPDSVPGEIRVFVDQTDEALRVIVEDDGAGFDPEGPARGTGLGTR
ILTAMAASLKSDFAYDPGHDGTKATLVFSLNEGKQGAV
>Mature_357_residues
STSSGDADAVIHILYIDDDEGLALLMQKNLGRRGFSVEWAENGAAGLARLGKGGFDAVVLDHVLAGETGLDILPCITALP
DHPPVIYATGSDDTSIAVAALKAGADDYMFKGISADYFDLLAAALEQALERARFRRETAQAQEVIRQQRDLAEMLLAEVN
HRIANSLGLVGALIRMQSSMTKDQVAIDALHETQMRINAIASVHRRLYTNRQVGSVQVDEYLNSLLTELEASMRDDKRPH
RVVLTAEPVNLATDKVITLGLIVSELVTNAFKYAYPDSVPGEIRVFVDQTDEALRVIVEDDGAGFDPEGPARGTGLGTRI
LTAMAASLKSDFAYDPGHDGTKATLVFSLNEGKQGAV

Specific function: Member of the two-component regulatory system pdtaR/pdtaS. Autophosphorylates, probably on a histidine residue, and transfers its phosphate group to pdtaR [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=28.6363636363636, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR022066
- InterPro:   IPR011495
- InterPro:   IPR005467 [H]

Pfam domain/function: PF12282 H_kinase_N; PF02518 HATPase_c; PF07568 HisKA_2 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38579; Mature: 38448

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSSGDADAVIHILYIDDDEGLALLMQKNLGRRGFSVEWAENGAAGLARLGKGGFDAVV
CCCCCCCCCEEEEEEEEECCCCEEEEEHHCCCCCCCEEEECCCCCHHHHHHCCCCCHHHH
LDHVLAGETGLDILPCITALPDHPPVIYATGSDDTSIAVAALKAGADDYMFKGISADYFD
HHHHHCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCEEEHHHHCCCCHHHHCCCCCHHHH
LLAAALEQALERARFRRETAQAQEVIRQQRDLAEMLLAEVNHRIANSLGLVGALIRMQSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTKDQVAIDALHETQMRINAIASVHRRLYTNRQVGSVQVDEYLNSLLTELEASMRDDKRP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCC
HRVVLTAEPVNLATDKVITLGLIVSELVTNAFKYAYPDSVPGEIRVFVDQTDEALRVIVE
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEE
DDGAGFDPEGPARGTGLGTRILTAMAASLKSDFAYDPGHDGTKATLVFSLNEGKQGAV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
STSSGDADAVIHILYIDDDEGLALLMQKNLGRRGFSVEWAENGAAGLARLGKGGFDAVV
CCCCCCCCEEEEEEEEECCCCEEEEEHHCCCCCCCEEEECCCCCHHHHHHCCCCCHHHH
LDHVLAGETGLDILPCITALPDHPPVIYATGSDDTSIAVAALKAGADDYMFKGISADYFD
HHHHHCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCEEEHHHHCCCCHHHHCCCCCHHHH
LLAAALEQALERARFRRETAQAQEVIRQQRDLAEMLLAEVNHRIANSLGLVGALIRMQSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTKDQVAIDALHETQMRINAIASVHRRLYTNRQVGSVQVDEYLNSLLTELEASMRDDKRP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCC
HRVVLTAEPVNLATDKVITLGLIVSELVTNAFKYAYPDSVPGEIRVFVDQTDEALRVIVE
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEEE
DDGAGFDPEGPARGTGLGTRILTAMAASLKSDFAYDPGHDGTKATLVFSLNEGKQGAV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]