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The map label for this gene is iciA [C]

Identifier: 209549736

GI number: 209549736

Start: 2204253

End: 2205146

Strand: Direct

Name: iciA [C]

Synonym: Rleg2_2144

Alternate gene names: 209549736

Gene position: 2204253-2205146 (Clockwise)

Preceding gene: 209549734

Following gene: 209549739

Centisome position: 48.57

GC content: 62.64

Gene sequence:

>894_bases
ATGCTCGATTATCCCGCCCTCCGCGCGGTCGCAACCGTCGTCCAAACAGGCAGCTTTGAACGCGCCGCCGCCATCCTCAA
CGTCACACCTTCGGCGATTTCCCAGCGCGTGAAGCAACTTGAAGAGCGTCTCGGCACCGTCCTCATCGTCAGAGGCACGC
CCTGCACGGCAACGGAAAAAGGAGAATGGCTCTGTCGTCACATGGAGAATGTCGGCATGTTGGAGGCCGAACTGCTCGGG
CAGTTGCCGGCGCTCGTCGATCCCGACGAGCCGCGCCAGCGGGTCACCCTTCAGATCGCAACCAATGCCGACAGTCTCGG
AACGTGGTTCGTCGAGGCCATGGCGAATTTCGCGAAGAACGCGTCCTACCTCCTGAACATCGCCGTCGACGACCAGGATC
ACACCGCCGAATGGCTTGAGCGCGGACGGGTGATCGCCGCCGTTACCAGTCTGGAAAAGCCGATCAGGGGATGCCGTCGT
TTCGAACTCGGCGTTCTCCGCTATCACGCCACAGCAAGCCCCGATTTCGTTGCGCGCTACTTTCCTGACGGCGTCACCGC
CGAAGCGATCCGCAATGCGCCGACGCTGACCTTCAATCAGAAGGACAGGTTGCAGAGCGGCTGGATAAGGCAGACCTTCG
GACACGATCTCGACCACCCCACCCACTGGCTGCCCTCGACGCAAAGCTTCGTCGAGGCCGCCCTTTCGGGTATGGGCTGG
GGAATGAACCCGACCCAGCTGACCCGCGAACATTTCGCCTCAGGCCGGCTGGTGGAACTCGTTCCCGATACGCCGCTGGA
TATTCCACTCTATTGGCAGATCAACCGCCTCGCCGCCGACCGCCTGGCGGAGCTGACGCGCGAAGTCGTCACGGTGGCGA
AGCGCCGCCTCTGA

Upstream 100 bases:

>100_bases
GCTTCGGCGAACACCCCTCACTTCGGTCGTGGCTGGGGTATCGCCATTTTCTGAATTAGGCTAGTTAATCTGACTTATCC
CAATTAAGCGATACTAATCC

Downstream 100 bases:

>100_bases
TTGCGATCAAACGTAAGCCGTGCGGCTGCCGAATTCGCTGGTTTCGTCGAAATTCTTCGTGCCGATACGGTCGCTTTCGC
CGGAGAGCTCCTGCGGAGGC

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLDYPALRAVATVVQTGSFERAAAILNVTPSAISQRVKQLEERLGTVLIVRGTPCTATEKGEWLCRHMENVGMLEAELLG
QLPALVDPDEPRQRVTLQIATNADSLGTWFVEAMANFAKNASYLLNIAVDDQDHTAEWLERGRVIAAVTSLEKPIRGCRR
FELGVLRYHATASPDFVARYFPDGVTAEAIRNAPTLTFNQKDRLQSGWIRQTFGHDLDHPTHWLPSTQSFVEAALSGMGW
GMNPTQLTREHFASGRLVELVPDTPLDIPLYWQINRLAADRLAELTREVVTVAKRRL

Sequences:

>Translated_297_residues
MLDYPALRAVATVVQTGSFERAAAILNVTPSAISQRVKQLEERLGTVLIVRGTPCTATEKGEWLCRHMENVGMLEAELLG
QLPALVDPDEPRQRVTLQIATNADSLGTWFVEAMANFAKNASYLLNIAVDDQDHTAEWLERGRVIAAVTSLEKPIRGCRR
FELGVLRYHATASPDFVARYFPDGVTAEAIRNAPTLTFNQKDRLQSGWIRQTFGHDLDHPTHWLPSTQSFVEAALSGMGW
GMNPTQLTREHFASGRLVELVPDTPLDIPLYWQINRLAADRLAELTREVVTVAKRRL
>Mature_297_residues
MLDYPALRAVATVVQTGSFERAAAILNVTPSAISQRVKQLEERLGTVLIVRGTPCTATEKGEWLCRHMENVGMLEAELLG
QLPALVDPDEPRQRVTLQIATNADSLGTWFVEAMANFAKNASYLLNIAVDDQDHTAEWLERGRVIAAVTSLEKPIRGCRR
FELGVLRYHATASPDFVARYFPDGVTAEAIRNAPTLTFNQKDRLQSGWIRQTFGHDLDHPTHWLPSTQSFVEAALSGMGW
GMNPTQLTREHFASGRLVELVPDTPLDIPLYWQINRLAADRLAELTREVVTVAKRRL

Specific function: Specific Inhibitor Of Chromosomal Initiation Of Replication In VItro. Binds The Three 13-Mers In The Origin (Oric) To Block Initiation Of Replication. [C]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=274, Percent_Identity=36.4963503649635, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33168; Mature: 33168

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYPALRAVATVVQTGSFERAAAILNVTPSAISQRVKQLEERLGTVLIVRGTPCTATEK
CCCCHHHHHHHHHHHCCCHHHHHHHEECCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCC
GEWLCRHMENVGMLEAELLGQLPALVDPDEPRQRVTLQIATNADSLGTWFVEAMANFAKN
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
ASYLLNIAVDDQDHTAEWLERGRVIAAVTSLEKPIRGCRRFELGVLRYHATASPDFVARY
CEEEEEEEECCCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHH
FPDGVTAEAIRNAPTLTFNQKDRLQSGWIRQTFGHDLDHPTHWLPSTQSFVEAALSGMGW
CCCCCCHHHHCCCCCEEECCHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
GMNPTQLTREHFASGRLVELVPDTPLDIPLYWQINRLAADRLAELTREVVTVAKRRL
CCCHHHHHHHHHCCCCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDYPALRAVATVVQTGSFERAAAILNVTPSAISQRVKQLEERLGTVLIVRGTPCTATEK
CCCCHHHHHHHHHHHCCCHHHHHHHEECCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCC
GEWLCRHMENVGMLEAELLGQLPALVDPDEPRQRVTLQIATNADSLGTWFVEAMANFAKN
HHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
ASYLLNIAVDDQDHTAEWLERGRVIAAVTSLEKPIRGCRRFELGVLRYHATASPDFVARY
CEEEEEEEECCCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHH
FPDGVTAEAIRNAPTLTFNQKDRLQSGWIRQTFGHDLDHPTHWLPSTQSFVEAALSGMGW
CCCCCCHHHHCCCCCEEECCHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCC
GMNPTQLTREHFASGRLVELVPDTPLDIPLYWQINRLAADRLAELTREVVTVAKRRL
CCCHHHHHHHHHCCCCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]