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The map label for this gene is cobS [H]

Identifier: 209549675

GI number: 209549675

Start: 2142211

End: 2142993

Strand: Direct

Name: cobS [H]

Synonym: Rleg2_2082

Alternate gene names: 209549675

Gene position: 2142211-2142993 (Clockwise)

Preceding gene: 209549672

Following gene: 209549676

Centisome position: 47.21

GC content: 67.56

Gene sequence:

>783_bases
ATGAAGATCAAGGACCATGCGGTCGATACCGCCCGCGCCGTCGCCTTCCTCAGCCGCATTCCCATGCCGCAATCGCTGTT
CAAAACCTATGACGGCAGGCTCGGCCGGCTGGTGCGCGCCTTTCCCTTCGCCGGCATCGTCATCGGCTTTGTTCCCGCGC
TCGCCCTGCTCCTGCTTCTGGGACTGCGCGCCGATCCGCTGATGGCGGCCCTGATCGCGCTTTCCATCCAGGTTGTCGTC
ACCGGCGCACTGCACGAGGACGGCCTGGCCGATACTGCCGACGGCATCGGCGGCGGCAAGAGCCGCGAGCAGAGCCTCGA
GATCATGAAGGACAGCCGGATCGGCACCTATGGCGCGATCGCGTTGATCCTCTCCTTCGCGATCCGCGCGGCAGCACTTG
CCGCCATCGCCCGCCATTCCCTGCCGCTTACATCAGTCCTTGCCATTCCCGCCGTCGCAGCGCTGAGCCGCGGCGCCATC
GCCTGGCACTGGCAGCGGCTGCCGCCGGCCAAGGCCGATGGCGTCGCCGCCTCGAACGGCCAGCCGGATGAGGGGGCGAT
GCAGTTTGCCCTCGCCTCGGCCGGCCTCGTCGTAGCGCTTCTGATCTGGCCTGCCTTCGGCCTGCGCCCGCTGGTCGCAA
GCCTGCTTGCCTCAGGCATCGCAGCGCTTGCCTTCACCGCCTTCATCCGCCGCAAGCTTGCCGGCCATACCGGCGACACG
CTGGGCGCGACGCAGCAAATTTGCGAGATCGCCGCGCTTTGCGCCCTTGCCACAGCTCTTTGA

Upstream 100 bases:

>100_bases
AACTCTCTTAAAGCGGGCTGACGGGGCGAACAACAGCAAAAGCGCCGCCGCGGCTATAAGCCAGCGCGATAAGCCGAAAC
CTTGGGCAGAACGAAGCAGA

Downstream 100 bases:

>100_bases
AACTCCGATATGTCCCTCATGCAAACACCCTGCATTCATGTCTGCTCGATCGAGCCCGCCACCGGATTTTGCGCCGGATG
CGGCCGGACTCTTCAGGAAA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKIKDHAVDTARAVAFLSRIPMPQSLFKTYDGRLGRLVRAFPFAGIVIGFVPALALLLLLGLRADPLMAALIALSIQVVV
TGALHEDGLADTADGIGGGKSREQSLEIMKDSRIGTYGAIALILSFAIRAAALAAIARHSLPLTSVLAIPAVAALSRGAI
AWHWQRLPPAKADGVAASNGQPDEGAMQFALASAGLVVALLIWPAFGLRPLVASLLASGIAALAFTAFIRRKLAGHTGDT
LGATQQICEIAALCALATAL

Sequences:

>Translated_260_residues
MKIKDHAVDTARAVAFLSRIPMPQSLFKTYDGRLGRLVRAFPFAGIVIGFVPALALLLLLGLRADPLMAALIALSIQVVV
TGALHEDGLADTADGIGGGKSREQSLEIMKDSRIGTYGAIALILSFAIRAAALAAIARHSLPLTSVLAIPAVAALSRGAI
AWHWQRLPPAKADGVAASNGQPDEGAMQFALASAGLVVALLIWPAFGLRPLVASLLASGIAALAFTAFIRRKLAGHTGDT
LGATQQICEIAALCALATAL
>Mature_260_residues
MKIKDHAVDTARAVAFLSRIPMPQSLFKTYDGRLGRLVRAFPFAGIVIGFVPALALLLLLGLRADPLMAALIALSIQVVV
TGALHEDGLADTADGIGGGKSREQSLEIMKDSRIGTYGAIALILSFAIRAAALAAIARHSLPLTSVLAIPAVAALSRGAI
AWHWQRLPPAKADGVAASNGQPDEGAMQFALASAGLVVALLIWPAFGLRPLVASLLASGIAALAFTAFIRRKLAGHTGDT
LGATQQICEIAALCALATAL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=245, Percent_Identity=33.0612244897959, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26854; Mature: 26854

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKDHAVDTARAVAFLSRIPMPQSLFKTYDGRLGRLVRAFPFAGIVIGFVPALALLLLL
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLRADPLMAALIALSIQVVVTGALHEDGLADTADGIGGGKSREQSLEIMKDSRIGTYGAI
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
ALILSFAIRAAALAAIARHSLPLTSVLAIPAVAALSRGAIAWHWQRLPPAKADGVAASNG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCC
QPDEGAMQFALASAGLVVALLIWPAFGLRPLVASLLASGIAALAFTAFIRRKLAGHTGDT
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LGATQQICEIAALCALATAL
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIKDHAVDTARAVAFLSRIPMPQSLFKTYDGRLGRLVRAFPFAGIVIGFVPALALLLLL
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLRADPLMAALIALSIQVVVTGALHEDGLADTADGIGGGKSREQSLEIMKDSRIGTYGAI
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
ALILSFAIRAAALAAIARHSLPLTSVLAIPAVAALSRGAIAWHWQRLPPAKADGVAASNG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCC
QPDEGAMQFALASAGLVVALLIWPAFGLRPLVASLLASGIAALAFTAFIRRKLAGHTGDT
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LGATQQICEIAALCALATAL
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA