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The map label for this gene is yafC [C]

Identifier: 209549667

GI number: 209549667

Start: 2133959

End: 2134855

Strand: Reverse

Name: yafC [C]

Synonym: Rleg2_2074

Alternate gene names: 209549667

Gene position: 2134855-2133959 (Counterclockwise)

Preceding gene: 209549668

Following gene: 209549664

Centisome position: 47.04

GC content: 62.1

Gene sequence:

>897_bases
ATGGACGATCTCAATGACTACTATTATTTCGCCGCTGTCGTCTCCAGCGGCGGCTTCGCCTCTGCAAGTCGCGATCTGAA
GATACCGCGATCGAAGCTGAGCAGACGGGTCAGCCGGCTCGAGGACGGGCTTGGGGCAAGGCTGATCGAACGCTCGACTC
GTCACTTCCGGGTGACGGAGATCGGCCAGGCTTTCTACGAAAGATGCCAGACCATCTTGCAGGAGGCCGACCGCGCCAAG
TCAATCGTCAGTGAGGCTCAATCCGATCCCCAAGGCGCGGTGCGGATGGGCTGCCCGCTCGGCCTCGTCGACATCTCGGT
CGGCGGAATCCTGCCGGAGTTCCTCGAGCGTTATCCAAAGATCAAACTGCAGATCATCGGCTCCGACCGGCGCGCCGACC
TCATCAACAAACGGATCGATCTTGAGGTGAGGGCGACCAACGAGCCGGAGACGCAGACGAGCCTGACGATGCGCAAGCTT
GACCGGGCGCGGCGCATCCTGGTCGCAAGTCCCTCGCTGGTCGAGCGCACCGGCAGCCTGAATTGCGTGGACGAGCTCGC
CGACCTTCCGACGCTGGCAATGACGTCATGGGTGTCTTTTCACAGTTGGGAATTGATCGGTCCAAATGACGCCAAACGAG
TGATTCGGCATCAGCCGCGGCTCACCTGCCGCAGCATGACCGCCATCCTTGACGCGGCCCGGGCCGGTCTCGGCTTCGGG
CTTTTGCTCGAGAGCGCCTGCGAAGCCGATCTCCAGGCCGGCAAGCTGGTGCGCGTGCTGCCGGAATGGCAGTCGGAGGA
GAGCCAGTTCTATATCGTCTTCACGACCGCCAAGGGCATGCCGCCGGCAGTGCGGGTGCTGATCGATTTCCTGGTCGAAA
AATCCCGGCAGCATTGA

Upstream 100 bases:

>100_bases
AAATCCAATGCCGACAATCTGATTTCAGCTTTTATGGTGAGGGGATACAACCTCTGCAGGGGGCCGGCATTCGTTTCCTT
CTCGCGGGCAGCTTGTTCTG

Downstream 100 bases:

>100_bases
TAAAGCGGCATTGACGAACAAGGGCTTCGCCTAGAAACCGGCATGCGCTTATTTCAGCCATGCCAGCAGCTTTTCGCCCG
CCAGTTCCTTGGCAGCATCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDDLNDYYYFAAVVSSGGFASASRDLKIPRSKLSRRVSRLEDGLGARLIERSTRHFRVTEIGQAFYERCQTILQEADRAK
SIVSEAQSDPQGAVRMGCPLGLVDISVGGILPEFLERYPKIKLQIIGSDRRADLINKRIDLEVRATNEPETQTSLTMRKL
DRARRILVASPSLVERTGSLNCVDELADLPTLAMTSWVSFHSWELIGPNDAKRVIRHQPRLTCRSMTAILDAARAGLGFG
LLLESACEADLQAGKLVRVLPEWQSEESQFYIVFTTAKGMPPAVRVLIDFLVEKSRQH

Sequences:

>Translated_298_residues
MDDLNDYYYFAAVVSSGGFASASRDLKIPRSKLSRRVSRLEDGLGARLIERSTRHFRVTEIGQAFYERCQTILQEADRAK
SIVSEAQSDPQGAVRMGCPLGLVDISVGGILPEFLERYPKIKLQIIGSDRRADLINKRIDLEVRATNEPETQTSLTMRKL
DRARRILVASPSLVERTGSLNCVDELADLPTLAMTSWVSFHSWELIGPNDAKRVIRHQPRLTCRSMTAILDAARAGLGFG
LLLESACEADLQAGKLVRVLPEWQSEESQFYIVFTTAKGMPPAVRVLIDFLVEKSRQH
>Mature_298_residues
MDDLNDYYYFAAVVSSGGFASASRDLKIPRSKLSRRVSRLEDGLGARLIERSTRHFRVTEIGQAFYERCQTILQEADRAK
SIVSEAQSDPQGAVRMGCPLGLVDISVGGILPEFLERYPKIKLQIIGSDRRADLINKRIDLEVRATNEPETQTSLTMRKL
DRARRILVASPSLVERTGSLNCVDELADLPTLAMTSWVSFHSWELIGPNDAKRVIRHQPRLTCRSMTAILDAARAGLGFG
LLLESACEADLQAGKLVRVLPEWQSEESQFYIVFTTAKGMPPAVRVLIDFLVEKSRQH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=29.4117647058824, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=27.891156462585, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=27.7027027027027, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789639, Length=302, Percent_Identity=26.8211920529801, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=24.6666666666667, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787589, Length=295, Percent_Identity=25.4237288135593, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=24.3150684931507, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI157672245, Length=117, Percent_Identity=29.9145299145299, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33334; Mature: 33334

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLNDYYYFAAVVSSGGFASASRDLKIPRSKLSRRVSRLEDGLGARLIERSTRHFRVTE
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCEEHHH
IGQAFYERCQTILQEADRAKSIVSEAQSDPQGAVRMGCPLGLVDISVGGILPEFLERYPK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHCCC
IKLQIIGSDRRADLINKRIDLEVRATNEPETQTSLTMRKLDRARRILVASPSLVERTGSL
EEEEEECCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCHHHHHCCCC
NCVDELADLPTLAMTSWVSFHSWELIGPNDAKRVIRHQPRLTCRSMTAILDAARAGLGFG
HHHHHHHCCCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHH
LLLESACEADLQAGKLVRVLPEWQSEESQFYIVFTTAKGMPPAVRVLIDFLVEKSRQH
HHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDDLNDYYYFAAVVSSGGFASASRDLKIPRSKLSRRVSRLEDGLGARLIERSTRHFRVTE
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCEEHHH
IGQAFYERCQTILQEADRAKSIVSEAQSDPQGAVRMGCPLGLVDISVGGILPEFLERYPK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHCCC
IKLQIIGSDRRADLINKRIDLEVRATNEPETQTSLTMRKLDRARRILVASPSLVERTGSL
EEEEEECCCHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCHHHHHCCCC
NCVDELADLPTLAMTSWVSFHSWELIGPNDAKRVIRHQPRLTCRSMTAILDAARAGLGFG
HHHHHHHCCCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHH
LLLESACEADLQAGKLVRVLPEWQSEESQFYIVFTTAKGMPPAVRVLIDFLVEKSRQH
HHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]