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The map label for this gene is trmJ [H]

Identifier: 209549544

GI number: 209549544

Start: 1993979

End: 1994809

Strand: Reverse

Name: trmJ [H]

Synonym: Rleg2_1950

Alternate gene names: 209549544

Gene position: 1994809-1993979 (Counterclockwise)

Preceding gene: 209549545

Following gene: 209549543

Centisome position: 43.96

GC content: 61.85

Gene sequence:

>831_bases
ATGGCAGGCACCAACAGCGAGCGTCAACTTCTGGCCGAAGGGCCGGCGATCATTCTGGTCGAGCCGCAAATGGGCGAGAA
TATCGGCATGGTGGCGCGCGCCATGGCGAATTTCGGCCTTGCTGAACTGCGCCTCGTTAATCCACGGGACGGCTGGCCGA
ACGAAAAAGCGCTGGCGACGGCCTCCAAGGCCGATCATGTGATCGCGGCAACGAAAGTCTACGACACGCTGGAGCAGGCC
GTCGCCGATCTCAACTTTGTCTATGCGACGACGGCGCGCGAGCGTGACGGTTATAAGCCGGTGCGATCCCCCGTGATCGC
CGCCGAGACCCTGAGGGCAAAATTCCGTGTGGGCGAGGGCACCGGCATCCTCTTCGGGCGCGAGCGCTGGGGGCTGACCA
ATGAGGAAGTGGCGCTTGCCGACGAGATCGTCACCTTTCCCGTCAACCCGGCTTTTGCTTCGCTGAACATCGCCCAGGCC
GTGCTGCTGATGTCCTATGAATGGATGAAGTCCGGCATGGAAGATGTCGGCGCGGTGCCGTTCCAGGCGATGGGGCAGAC
ACCCTCGACCAAGGAGCAACTGTTCGGCCTGTTCGACCAATTGGAAGAGGCGCTGGATGCGCGCGGATATTTCCATCCTG
CCGGGAAAAAGCCGAAAATGGTCGATAATCTGCGTGCTGTTCTATCCCGCCGGGCTTTCACGGAGCAAGAAATTAGCGTG
CTGCGCGGCGTCATCTCCTCCCTCGACCGCTTTTCGCGCAAGAGCCCGCGCGGTGGCAGGTTCCCGACACCGGCCAAGGA
AACACCGCCAGATGACAGCGACGACGCGTGA

Upstream 100 bases:

>100_bases
GAAATGATCGGTTCGGCGCTGGAAAGGCTCTATCCGCAGGCCGACGTCTTGCCGTTTGCGACGGGCGAGGCTATTGCGCG
CTCGAAACAAGGATAAGACG

Downstream 100 bases:

>100_bases
GCTGAAACCTATCCTTCTCTTCGATTCCGGCATCGGCGGGCTGACCGTCTTGCGCGAGGCGCGCGTCTTGATGCCTGAGC
GCGGCTTCATCTATGTCGCC

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAGTNSERQLLAEGPAIILVEPQMGENIGMVARAMANFGLAELRLVNPRDGWPNEKALATASKADHVIAATKVYDTLEQA
VADLNFVYATTARERDGYKPVRSPVIAAETLRAKFRVGEGTGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQA
VLLMSYEWMKSGMEDVGAVPFQAMGQTPSTKEQLFGLFDQLEEALDARGYFHPAGKKPKMVDNLRAVLSRRAFTEQEISV
LRGVISSLDRFSRKSPRGGRFPTPAKETPPDDSDDA

Sequences:

>Translated_276_residues
MAGTNSERQLLAEGPAIILVEPQMGENIGMVARAMANFGLAELRLVNPRDGWPNEKALATASKADHVIAATKVYDTLEQA
VADLNFVYATTARERDGYKPVRSPVIAAETLRAKFRVGEGTGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQA
VLLMSYEWMKSGMEDVGAVPFQAMGQTPSTKEQLFGLFDQLEEALDARGYFHPAGKKPKMVDNLRAVLSRRAFTEQEISV
LRGVISSLDRFSRKSPRGGRFPTPAKETPPDDSDDA
>Mature_275_residues
AGTNSERQLLAEGPAIILVEPQMGENIGMVARAMANFGLAELRLVNPRDGWPNEKALATASKADHVIAATKVYDTLEQAV
ADLNFVYATTARERDGYKPVRSPVIAAETLRAKFRVGEGTGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQAV
LLMSYEWMKSGMEDVGAVPFQAMGQTPSTKEQLFGLFDQLEEALDARGYFHPAGKKPKMVDNLRAVLSRRAFTEQEISVL
RGVISSLDRFSRKSPRGGRFPTPAKETPPDDSDDA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=251, Percent_Identity=32.6693227091633, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790865, Length=152, Percent_Identity=39.4736842105263, Blast_Score=98, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30187; Mature: 30055

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTNSERQLLAEGPAIILVEPQMGENIGMVARAMANFGLAELRLVNPRDGWPNEKALAT
CCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHH
ASKADHVIAATKVYDTLEQAVADLNFVYATTARERDGYKPVRSPVIAAETLRAKFRVGEG
HCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHCHHHHHHHHHHHEEECCC
TGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQAVLLMSYEWMKSGMEDVGAVP
CEEEEECHHCCCCCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
FQAMGQTPSTKEQLFGLFDQLEEALDARGYFHPAGKKPKMVDNLRAVLSRRAFTEQEISV
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
LRGVISSLDRFSRKSPRGGRFPTPAKETPPDDSDDA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGTNSERQLLAEGPAIILVEPQMGENIGMVARAMANFGLAELRLVNPRDGWPNEKALAT
CCCCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHH
ASKADHVIAATKVYDTLEQAVADLNFVYATTARERDGYKPVRSPVIAAETLRAKFRVGEG
HCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHCHHHHHHHHHHHEEECCC
TGILFGRERWGLTNEEVALADEIVTFPVNPAFASLNIAQAVLLMSYEWMKSGMEDVGAVP
CEEEEECHHCCCCCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
FQAMGQTPSTKEQLFGLFDQLEEALDARGYFHPAGKKPKMVDNLRAVLSRRAFTEQEISV
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
LRGVISSLDRFSRKSPRGGRFPTPAKETPPDDSDDA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA