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The map label for this gene is tyrS [H]

Identifier: 209549500

GI number: 209549500

Start: 1950352

End: 1951608

Strand: Direct

Name: tyrS [H]

Synonym: Rleg2_1906

Alternate gene names: 209549500

Gene position: 1950352-1951608 (Clockwise)

Preceding gene: 209549497

Following gene: 209549502

Centisome position: 42.98

GC content: 61.73

Gene sequence:

>1257_bases
ATGTCCGAGTTCAAGTCCGATTTCCTCCGCACGCTGAAAGAGCGCGGCTTCATTCATCAGGTCTCCGATGAACGCGGCCT
CGACGATATGTTCGCCAAGGAAACCGTGACGGCCTATATCGGTTACGACCCGACGGCATCCAGCCTCCATGTCGGCCATC
TCACCCAGATCATGATGCTGCACTGGCTGCAGGCGACGGGCCACCGCCCGATTTCGCTGATGGGCGGCGGCACCGGCATG
GTCGGCGATCCCTCCTTCAAGGAGGAGGCCCGCCAGCTGATGACGATCGACACGATCGAGCAGAATATCGCCTCGATCAA
GAACTGCTTCTCGAACTATCTCGACTACGACAAGTCAGGCAATGCCGCGCTGATGATCAACAATGCCGAATGGCTGCGTT
CGTTGAATTACCTCGAGTTCCTGCGCGATGTCGGCCGTCATTTCTCGGTCAACCGCATGCTGTCCTTCGACAGCGTGAAG
ACGCGCCTTGACCGCGAACAGTCGCTGTCCTTCCTGGAATTCAACTACATGATCCTCCAGGCCTACGACTTCGTCGAGCT
TGCCAAGCGCTACGACTGCCGTCTGCAGATGGGCGGATCGGATCAGTGGGGCAATATCGTCAACGGCATCGATCTCGGTC
ACCGCATGGGGACACAGCAGCTCTACGCGCTGACTTCGCCGCTGCTGACGACGTCATCCGGCGCCAAGATGGGCAAGTCG
GCGTCCGGCGCGGTCTGGCTGAATGCCGACCTGCTCCCCGTCTATGACTTCTGGCAATATTGGCGCAACACCGAGGATGC
CGACGTGCCGCGCTTCCTCAAGCTCTTCACCACGCTGCCGATGGATGAAATCGCACGGCTTTCGGCACTCGGCGGTTCGG
AGCTGAACGAGGTCAAGAAGATCCTGGCAACCGAAGTTACCACAATCCTGCACGGCCGCCCGGCCGCCGAGCAGGCCGCG
GAAACGGCGCGCAAGACCTTCGAAGAAGGCGGGCTCTCGGAAAACCTGCCGTCGGTCGATATCCCCGCCACCGAACTCGA
CGGCGGCATCGGCCTGCTCTCGCTGATGGTCCGCGCCGGCCTTGCCGGCTCGAACGGCGAAGCCCGCCGCCACGTCCAGG
GCGGCGCGGTCCGCGTCAACGACGAGGCGGTCAGCGACGAACGCCGTCTGATCGGCAGCAGTGAAATCACCGCCGACGGC
GTCATCAAGCTCTCGCTGGGTAAAAAGAAGCACATCCTGATCCGCCGCGCCGCGTAA

Upstream 100 bases:

>100_bases
ACTTTTATAAAAACAGTGCTAAACGCGCCGCGACAGATCAACAACAACGAACTTGCGTAAAGCCATATGGGTCAAGCGCA
GACACCAAGAGACGAAAGCC

Downstream 100 bases:

>100_bases
GCGGATTGAGTGTCGAACGGATATCAAAGCCTGCGTCCGCGCCGGCTTTTCTTTGTCCAACTCCTGAATTGGCGGGTAAC
CCTCACTCAACGCCTGGCCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSEFKSDFLRTLKERGFIHQVSDERGLDDMFAKETVTAYIGYDPTASSLHVGHLTQIMMLHWLQATGHRPISLMGGGTGM
VGDPSFKEEARQLMTIDTIEQNIASIKNCFSNYLDYDKSGNAALMINNAEWLRSLNYLEFLRDVGRHFSVNRMLSFDSVK
TRLDREQSLSFLEFNYMILQAYDFVELAKRYDCRLQMGGSDQWGNIVNGIDLGHRMGTQQLYALTSPLLTTSSGAKMGKS
ASGAVWLNADLLPVYDFWQYWRNTEDADVPRFLKLFTTLPMDEIARLSALGGSELNEVKKILATEVTTILHGRPAAEQAA
ETARKTFEEGGLSENLPSVDIPATELDGGIGLLSLMVRAGLAGSNGEARRHVQGGAVRVNDEAVSDERRLIGSSEITADG
VIKLSLGKKKHILIRRAA

Sequences:

>Translated_418_residues
MSEFKSDFLRTLKERGFIHQVSDERGLDDMFAKETVTAYIGYDPTASSLHVGHLTQIMMLHWLQATGHRPISLMGGGTGM
VGDPSFKEEARQLMTIDTIEQNIASIKNCFSNYLDYDKSGNAALMINNAEWLRSLNYLEFLRDVGRHFSVNRMLSFDSVK
TRLDREQSLSFLEFNYMILQAYDFVELAKRYDCRLQMGGSDQWGNIVNGIDLGHRMGTQQLYALTSPLLTTSSGAKMGKS
ASGAVWLNADLLPVYDFWQYWRNTEDADVPRFLKLFTTLPMDEIARLSALGGSELNEVKKILATEVTTILHGRPAAEQAA
ETARKTFEEGGLSENLPSVDIPATELDGGIGLLSLMVRAGLAGSNGEARRHVQGGAVRVNDEAVSDERRLIGSSEITADG
VIKLSLGKKKHILIRRAA
>Mature_417_residues
SEFKSDFLRTLKERGFIHQVSDERGLDDMFAKETVTAYIGYDPTASSLHVGHLTQIMMLHWLQATGHRPISLMGGGTGMV
GDPSFKEEARQLMTIDTIEQNIASIKNCFSNYLDYDKSGNAALMINNAEWLRSLNYLEFLRDVGRHFSVNRMLSFDSVKT
RLDREQSLSFLEFNYMILQAYDFVELAKRYDCRLQMGGSDQWGNIVNGIDLGHRMGTQQLYALTSPLLTTSSGAKMGKSA
SGAVWLNADLLPVYDFWQYWRNTEDADVPRFLKLFTTLPMDEIARLSALGGSELNEVKKILATEVTTILHGRPAAEQAAE
TARKTFEEGGLSENLPSVDIPATELDGGIGLLSLMVRAGLAGSNGEARRHVQGGAVRVNDEAVSDERRLIGSSEITADGV
IKLSLGKKKHILIRRAA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=432, Percent_Identity=33.3333333333333, Blast_Score=219, Evalue=5e-57,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=40.5727923627685, Blast_Score=333, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25145802, Length=375, Percent_Identity=31.7333333333333, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25145805, Length=293, Percent_Identity=30.3754266211604, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325160, Length=442, Percent_Identity=35.5203619909502, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI20130383, Length=435, Percent_Identity=33.1034482758621, Blast_Score=242, Evalue=3e-64,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46395; Mature: 46264

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFKSDFLRTLKERGFIHQVSDERGLDDMFAKETVTAYIGYDPTASSLHVGHLTQIMML
CCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHEEEEECCCCCCCCEEHHHHHHHHHH
HWLQATGHRPISLMGGGTGMVGDPSFKEEARQLMTIDTIEQNIASIKNCFSNYLDYDKSG
HHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NAALMINNAEWLRSLNYLEFLRDVGRHFSVNRMLSFDSVKTRLDREQSLSFLEFNYMILQ
CEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AYDFVELAKRYDCRLQMGGSDQWGNIVNGIDLGHRMGTQQLYALTSPLLTTSSGAKMGKS
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHCHHCCCCCCCCCCC
ASGAVWLNADLLPVYDFWQYWRNTEDADVPRFLKLFTTLPMDEIARLSALGGSELNEVKK
CCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
ILATEVTTILHGRPAAEQAAETARKTFEEGGLSENLPSVDIPATELDGGIGLLSLMVRAG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHC
LAGSNGEARRHVQGGAVRVNDEAVSDERRLIGSSEITADGVIKLSLGKKKHILIRRAA
CCCCCCCHHHHCCCCEEEECCHHHCHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECC
>Mature Secondary Structure 
SEFKSDFLRTLKERGFIHQVSDERGLDDMFAKETVTAYIGYDPTASSLHVGHLTQIMML
CHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHEEEEECCCCCCCCEEHHHHHHHHHH
HWLQATGHRPISLMGGGTGMVGDPSFKEEARQLMTIDTIEQNIASIKNCFSNYLDYDKSG
HHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NAALMINNAEWLRSLNYLEFLRDVGRHFSVNRMLSFDSVKTRLDREQSLSFLEFNYMILQ
CEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AYDFVELAKRYDCRLQMGGSDQWGNIVNGIDLGHRMGTQQLYALTSPLLTTSSGAKMGKS
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHCHHCCCCCCCCCCC
ASGAVWLNADLLPVYDFWQYWRNTEDADVPRFLKLFTTLPMDEIARLSALGGSELNEVKK
CCCEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
ILATEVTTILHGRPAAEQAAETARKTFEEGGLSENLPSVDIPATELDGGIGLLSLMVRAG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHC
LAGSNGEARRHVQGGAVRVNDEAVSDERRLIGSSEITADGVIKLSLGKKKHILIRRAA
CCCCCCCHHHHCCCCEEEECCHHHCHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]