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The map label for this gene is gcvH [H]

Identifier: 209549484

GI number: 209549484

Start: 1933911

End: 1934273

Strand: Direct

Name: gcvH [H]

Synonym: Rleg2_1890

Alternate gene names: 209549484

Gene position: 1933911-1934273 (Clockwise)

Preceding gene: 209549483

Following gene: 209549485

Centisome position: 42.62

GC content: 57.85

Gene sequence:

>363_bases
ATGCTGAAATTTACCGAAGAACACGAATGGCTGCAGATCGAAGGCAGCGTTGCGACCGTTGGTATCACCAATTATGCCGT
CGATCAGCTCGGCGACCTGGTTTTCGTCGAACTGCCGGAAGTCGGCGCAACCTTCTCCAAGAACGGCAATGCCGCGACCG
TTGAATCCGTCAAGGCGGCGTCCGATGTCTATTGTCCGCTTGACGGTGAGATCACCGAGGTCAATCCGGCGATCGTTGCC
GATCCGTCGCTGGTCAATTCCGATCCTCAAGGGGCGGGCTGGTTCTTCAAGCTGAAGCTTGCCAATCCCGCGGATGCCGA
CGGCTTGCTCGACGAGGCCGCCTATAAGGAGCTCACCGCGTAA

Upstream 100 bases:

>100_bases
ACGCCGAAGTGCGCGGCAAATATTTGCCGATCACCGTCAGCGCCCTGCCCTTCGTCACACCGACCTACAAACGCTAAACC
TTTTCCAGAGAGGACGAACA

Downstream 100 bases:

>100_bases
TGACGACGCCGACCGAATTCCAGTTTACCGACTATCAGCCTTACGATTTTGCCAATCGCCGTCATATCGGCCCCTCGCCG
GCCGAGATGACCGATATGCT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MLKFTEEHEWLQIEGSVATVGITNYAVDQLGDLVFVELPEVGATFSKNGNAATVESVKAASDVYCPLDGEITEVNPAIVA
DPSLVNSDPQGAGWFFKLKLANPADADGLLDEAAYKELTA

Sequences:

>Translated_120_residues
MLKFTEEHEWLQIEGSVATVGITNYAVDQLGDLVFVELPEVGATFSKNGNAATVESVKAASDVYCPLDGEITEVNPAIVA
DPSLVNSDPQGAGWFFKLKLANPADADGLLDEAAYKELTA
>Mature_120_residues
MLKFTEEHEWLQIEGSVATVGITNYAVDQLGDLVFVELPEVGATFSKNGNAATVESVKAASDVYCPLDGEITEVNPAIVA
DPSLVNSDPQGAGWFFKLKLANPADADGLLDEAAYKELTA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=115, Percent_Identity=47.8260869565217, Blast_Score=122, Evalue=8e-29,
Organism=Homo sapiens, GI89057342, Length=115, Percent_Identity=47.8260869565217, Blast_Score=122, Evalue=9e-29,
Organism=Escherichia coli, GI1789271, Length=118, Percent_Identity=52.5423728813559, Blast_Score=124, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=44.6428571428571, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=117, Percent_Identity=41.8803418803419, Blast_Score=112, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=41.6666666666667, Blast_Score=106, Evalue=9e-25,
Organism=Drosophila melanogaster, GI17865652, Length=115, Percent_Identity=43.4782608695652, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 12745; Mature: 12745

Theoretical pI: Translated: 3.78; Mature: 3.78

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFTEEHEWLQIEGSVATVGITNYAVDQLGDLVFVELPEVGATFSKNGNAATVESVKAA
CCCCCCCCCEEEEECCEEEEEECHHHHHHCCCEEEEEECCCCCEECCCCCEEEHHHHHCC
SDVYCPLDGEITEVNPAIVADPSLVNSDPQGAGWFFKLKLANPADADGLLDEAAYKELTA
CCEECCCCCCEEECCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLKFTEEHEWLQIEGSVATVGITNYAVDQLGDLVFVELPEVGATFSKNGNAATVESVKAA
CCCCCCCCCEEEEECCEEEEEECHHHHHHCCCEEEEEECCCCCEECCCCCEEEHHHHHCC
SDVYCPLDGEITEVNPAIVADPSLVNSDPQGAGWFFKLKLANPADADGLLDEAAYKELTA
CCEECCCCCCEEECCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA