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The map label for this gene is gyrB [H]

Identifier: 209549430

GI number: 209549430

Start: 1884424

End: 1886538

Strand: Direct

Name: gyrB [H]

Synonym: Rleg2_1836

Alternate gene names: 209549430

Gene position: 1884424-1886538 (Clockwise)

Preceding gene: 209549424

Following gene: 209549431

Centisome position: 41.53

GC content: 64.26

Gene sequence:

>2115_bases
ATGGACGATAGCAACGACCTCTTTTCCGGAATGCCCCTCTCTGAAAAACGTGAGGAGGCGCAGAAGCCTGCGGCTGCGGC
CGAACGGCCGCCGGTGGCGGCTGCGCCCGCTGCTGCCCCACCTGCTGCTGGAGCCCCCTCTGCTGCGGGAGCCTCTTCGC
GCCCGGCACCCACCACGTCGAACGCCGACGAATACGGCGCCTCGTCGATCCGCGTCCTCGAAGGCCTCGAGCCGGTGCGC
ATGCGCCCGGGCATGTATATCGGCGGCACCGATGAAAAGGCGCTGCATCACCTCTTTGCCGAAGTCATCGACAACGCGAT
GGACGAGGCGGTCGCCGGACACGCCAATTTCATCGAGGTCTATCTCGACCTCGAGGGTTACCTCACCGTCTCCGACAACG
GCCGCGGCATCCCGGTCGAGAACCATCCGCAGGTGCCGGGTAAGTCCACGCTCGAAGTCATCATGACCAAGCTGCATGCC
GGCGGCAAATTCGACGGCAAGGCCTACGAGACCTCGGGCGGCCTGCATGGCGTCGGCGTCTCCGTCGTCAACGCGCTCTC
TGACGATCTCGAAGTGGAGGTCGCGCGCAACCGCAAGCTCTACCGCCAGCGCTTCTCCCGCGGCCTGCCGCAGGGCGGGC
TTGAAGAATTGGGCGACGTCCACAATCGCCGCGGCACCCGCGTGCGCTTCCATCCCGACCCCCAGATCTTTGGGGATCAC
ATGAAGTTCGATGCCGCCCGGGTGTTCCGCATGGCGCGCTCGAAGGCCTATCTGTTCGGCGGCGTCGAGATCCGCTGGAG
CTGCGAGCAGGGCGTCTTGCCCGAAGGCTCCGAGGTCCCTGACAAGGCGGTCTTCCACTTCCCCGGCGGCCTGAAGGATT
ATCTCCAGGCGACGATGGGCAAGGAATTCACCGTCACCCGCGAGATCTTCGCCGGCAAGACCGAGAAAACCAGCGGCCAT
GGCTCGATGGAATGGGCGATCACCTGGTATGGCGGCGATCCGCAGGTGCATTCCTATTGCAACACCATCCCGACCCCCGA
AGGCGGCACGCATGAGGCCGGCCTGCGCATCGCGCTGACCAAGGGCCTGAAGGCCTATGCCGAGCTGACGCAGAACAAGC
GCGCCGCGCAGATCACCACGGATGACGTGATGATCTCGGCCGTCGGCATGCTGTCGGTCTTCATCCGCGAACCGGAATTC
GTCGGCCAGACCAAGGATAAGCTCGCGACCGTCGAGGCCCAGCGCATCGTCGAGAACGCGCTACGCGATCCTTTCGACCA
CTACCTTGCCGACAATCCGAACGAGTCGGCCAAGCTGCTCGACTGGGTGATCGAGCGCGCCGAGGAGCGCCTGCGCCGCC
GCAAGGAAAAGGAAGTCAACCGCAAGACCGCGGTGCGTAAGCTGCGCCTGCCCGGCAAGCTCGCCGACTGCTCGCAGAAC
ACCGCAGAAGGCGCTGAACTCTTCATCGTCGAAGGTGACTCGGCAGGCGGTTCGGCCAAGCAGGCGCGCAACCGCGCCAA
CCAGGCAATTCTGCCGCTGCGCGGCAAGATCCTCAACGTTGCCAGCGCCGGCCGCGAAAAACTCGGCGCCAACCAGCAGC
TCGCCGATCTCATCCAGGCGCTCGGCTGCGGCACCCGCTCGAAATACCGCGACGAAGATCTGCGCTACGAGCGCATCATC
GTCATGACCGATGCCGACGTCGACGGCGCCCACATCGCCTCGCTGCTCATCACCTTCTTCTATCAGGAGATGCCGGAACT
GGTGCGCGGCGGCCACCTCTTCCTAGCCGTGCCGCCGCTCTACAAGATCACCCAAGGGTCGAAATCCGCCTATGCCCGCG
ACGACAATCATCGCGCCGAGCTGATGCAGACGGAGTTCAAGGGCAAGGCCAAGGTCGAGATCAGCCGCTTCAAAGGTCTC
GGCGAAATGATGCCCGCCCAGCTCAAGGAAACCACGATGGATCCGTCCAAACGCACCCTGCTCAAGGTGCTGATCGACGA
GGTGGATTTCGAGGGCACTCGCAGCGCCGTCGACGATCTGATGGGCACCAAGCCGGAAGCCCGCTTCCGCTTCATCCAGG
AACGCGCGGCCTTCGCCGAAAACCTCGATATTTAA

Upstream 100 bases:

>100_bases
CCTGATTCGCAAACGTGACATGAAATGCGGTGATTTTGGGTCGGCGGAGGATGCAAGCCCCAAGCCAGACCCAAGACAGG
ATGAAGATCTGAACGGCTCC

Downstream 100 bases:

>100_bases
GGCATCTGCGGGCCGAGCCGAACGGGAGTAGATACCGTTTGGCGCGGCCCTCATTTTAGCATCTGCGATTTCACGGAACT
GACGAAGAACCGTAACAACT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MDDSNDLFSGMPLSEKREEAQKPAAAAERPPVAAAPAAAPPAAGAPSAAGASSRPAPTTSNADEYGASSIRVLEGLEPVR
MRPGMYIGGTDEKALHHLFAEVIDNAMDEAVAGHANFIEVYLDLEGYLTVSDNGRGIPVENHPQVPGKSTLEVIMTKLHA
GGKFDGKAYETSGGLHGVGVSVVNALSDDLEVEVARNRKLYRQRFSRGLPQGGLEELGDVHNRRGTRVRFHPDPQIFGDH
MKFDAARVFRMARSKAYLFGGVEIRWSCEQGVLPEGSEVPDKAVFHFPGGLKDYLQATMGKEFTVTREIFAGKTEKTSGH
GSMEWAITWYGGDPQVHSYCNTIPTPEGGTHEAGLRIALTKGLKAYAELTQNKRAAQITTDDVMISAVGMLSVFIREPEF
VGQTKDKLATVEAQRIVENALRDPFDHYLADNPNESAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGKLADCSQN
TAEGAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVASAGREKLGANQQLADLIQALGCGTRSKYRDEDLRYERII
VMTDADVDGAHIASLLITFFYQEMPELVRGGHLFLAVPPLYKITQGSKSAYARDDNHRAELMQTEFKGKAKVEISRFKGL
GEMMPAQLKETTMDPSKRTLLKVLIDEVDFEGTRSAVDDLMGTKPEARFRFIQERAAFAENLDI

Sequences:

>Translated_704_residues
MDDSNDLFSGMPLSEKREEAQKPAAAAERPPVAAAPAAAPPAAGAPSAAGASSRPAPTTSNADEYGASSIRVLEGLEPVR
MRPGMYIGGTDEKALHHLFAEVIDNAMDEAVAGHANFIEVYLDLEGYLTVSDNGRGIPVENHPQVPGKSTLEVIMTKLHA
GGKFDGKAYETSGGLHGVGVSVVNALSDDLEVEVARNRKLYRQRFSRGLPQGGLEELGDVHNRRGTRVRFHPDPQIFGDH
MKFDAARVFRMARSKAYLFGGVEIRWSCEQGVLPEGSEVPDKAVFHFPGGLKDYLQATMGKEFTVTREIFAGKTEKTSGH
GSMEWAITWYGGDPQVHSYCNTIPTPEGGTHEAGLRIALTKGLKAYAELTQNKRAAQITTDDVMISAVGMLSVFIREPEF
VGQTKDKLATVEAQRIVENALRDPFDHYLADNPNESAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGKLADCSQN
TAEGAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVASAGREKLGANQQLADLIQALGCGTRSKYRDEDLRYERII
VMTDADVDGAHIASLLITFFYQEMPELVRGGHLFLAVPPLYKITQGSKSAYARDDNHRAELMQTEFKGKAKVEISRFKGL
GEMMPAQLKETTMDPSKRTLLKVLIDEVDFEGTRSAVDDLMGTKPEARFRFIQERAAFAENLDI
>Mature_704_residues
MDDSNDLFSGMPLSEKREEAQKPAAAAERPPVAAAPAAAPPAAGAPSAAGASSRPAPTTSNADEYGASSIRVLEGLEPVR
MRPGMYIGGTDEKALHHLFAEVIDNAMDEAVAGHANFIEVYLDLEGYLTVSDNGRGIPVENHPQVPGKSTLEVIMTKLHA
GGKFDGKAYETSGGLHGVGVSVVNALSDDLEVEVARNRKLYRQRFSRGLPQGGLEELGDVHNRRGTRVRFHPDPQIFGDH
MKFDAARVFRMARSKAYLFGGVEIRWSCEQGVLPEGSEVPDKAVFHFPGGLKDYLQATMGKEFTVTREIFAGKTEKTSGH
GSMEWAITWYGGDPQVHSYCNTIPTPEGGTHEAGLRIALTKGLKAYAELTQNKRAAQITTDDVMISAVGMLSVFIREPEF
VGQTKDKLATVEAQRIVENALRDPFDHYLADNPNESAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGKLADCSQN
TAEGAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVASAGREKLGANQQLADLIQALGCGTRSKYRDEDLRYERII
VMTDADVDGAHIASLLITFFYQEMPELVRGGHLFLAVPPLYKITQGSKSAYARDDNHRAELMQTEFKGKAKVEISRFKGL
GEMMPAQLKETTMDPSKRTLLKVLIDEVDFEGTRSAVDDLMGTKPEARFRFIQERAAFAENLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=619, Percent_Identity=26.4943457189015, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI19913408, Length=632, Percent_Identity=24.6835443037975, Blast_Score=136, Evalue=6e-32,
Organism=Escherichia coli, GI48994957, Length=560, Percent_Identity=44.6428571428571, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789408, Length=645, Percent_Identity=40.1550387596899, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17535065, Length=623, Percent_Identity=24.0770465489567, Blast_Score=129, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=597, Percent_Identity=23.785594639866, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=212, Percent_Identity=26.4150943396226, Blast_Score=70, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=630, Percent_Identity=25.0793650793651, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17136538, Length=636, Percent_Identity=24.8427672955975, Blast_Score=129, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 77248; Mature: 77248

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDSNDLFSGMPLSEKREEAQKPAAAAERPPVAAAPAAAPPAAGAPSAAGASSRPAPTTS
CCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NADEYGASSIRVLEGLEPVRMRPGMYIGGTDEKALHHLFAEVIDNAMDEAVAGHANFIEV
CCHHHCCCHHHHHHCCCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
YLDLEGYLTVSDNGRGIPVENHPQVPGKSTLEVIMTKLHAGGKFDGKAYETSGGLHGVGV
EEEECEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHH
SVVNALSDDLEVEVARNRKLYRQRFSRGLPQGGLEELGDVHNRRGTRVRFHPDPQIFGDH
HHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHCCH
MKFDAARVFRMARSKAYLFGGVEIRWSCEQGVLPEGSEVPDKAVFHFPGGLKDYLQATMG
HHHHHHHHHHHHHCCEEEEECEEEEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCC
KEFTVTREIFAGKTEKTSGHGSMEWAITWYGGDPQVHSYCNTIPTPEGGTHEAGLRIALT
CCEEEHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCEEEEEH
KGLKAYAELTQNKRAAQITTDDVMISAVGMLSVFIREPEFVGQTKDKLATVEAQRIVENA
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHH
LRDPFDHYLADNPNESAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGKLADCSQN
HHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
TAEGAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVASAGREKLGANQQLADLIQA
CCCCCEEEEEECCCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHH
LGCGTRSKYRDEDLRYERIIVMTDADVDGAHIASLLITFFYQEMPELVRGGHLFLAVPPL
HCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
YKITQGSKSAYARDDNHRAELMQTEFKGKAKVEISRFKGLGEMMPAQLKETTMDPSKRTL
CEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHCCCCHHHHH
LKVLIDEVDFEGTRSAVDDLMGTKPEARFRFIQERAAFAENLDI
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDDSNDLFSGMPLSEKREEAQKPAAAAERPPVAAAPAAAPPAAGAPSAAGASSRPAPTTS
CCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NADEYGASSIRVLEGLEPVRMRPGMYIGGTDEKALHHLFAEVIDNAMDEAVAGHANFIEV
CCHHHCCCHHHHHHCCCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
YLDLEGYLTVSDNGRGIPVENHPQVPGKSTLEVIMTKLHAGGKFDGKAYETSGGLHGVGV
EEEECEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHH
SVVNALSDDLEVEVARNRKLYRQRFSRGLPQGGLEELGDVHNRRGTRVRFHPDPQIFGDH
HHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHCCH
MKFDAARVFRMARSKAYLFGGVEIRWSCEQGVLPEGSEVPDKAVFHFPGGLKDYLQATMG
HHHHHHHHHHHHHCCEEEEECEEEEEECCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCC
KEFTVTREIFAGKTEKTSGHGSMEWAITWYGGDPQVHSYCNTIPTPEGGTHEAGLRIALT
CCEEEHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCEEEEEH
KGLKAYAELTQNKRAAQITTDDVMISAVGMLSVFIREPEFVGQTKDKLATVEAQRIVENA
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHH
LRDPFDHYLADNPNESAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGKLADCSQN
HHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCC
TAEGAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVASAGREKLGANQQLADLIQA
CCCCCEEEEEECCCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHH
LGCGTRSKYRDEDLRYERIIVMTDADVDGAHIASLLITFFYQEMPELVRGGHLFLAVPPL
HCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
YKITQGSKSAYARDDNHRAELMQTEFKGKAKVEISRFKGLGEMMPAQLKETTMDPSKRTL
CEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHCCCCHHHHH
LKVLIDEVDFEGTRSAVDDLMGTKPEARFRFIQERAAFAENLDI
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]