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The map label for this gene is citA [H]

Identifier: 209549424

GI number: 209549424

Start: 1878271

End: 1879359

Strand: Direct

Name: citA [H]

Synonym: Rleg2_1830

Alternate gene names: 209549424

Gene position: 1878271-1879359 (Clockwise)

Preceding gene: 209549418

Following gene: 209549430

Centisome position: 41.39

GC content: 66.85

Gene sequence:

>1089_bases
ATGAAAAATGGCTTGGAAGACGTCATTGCCGCCGAAACGCAGCTCTCGGATGTCGATGGCGAAGCGGGGCGGCTGATCAT
CCGCGGCGTCTCGCTGGACCATCTGGTTGCAGACTGCAGCTATGAAGGTGTCGCCGCATTGCTGCTCGACGGGCTGATCG
AAAGAAGCTTCGATCAGGCGCAGTTGCGCGCGTGGCTGGCGAATGCCCGAACACGGATTTTCCACCACATCGGGGCCGCC
GATGCCGCTCTGCTGACGCTGCCGCCGGTTGATGCGATGCGGGCGCTGATCGCCCGACTGCCCGACGGCGAGGATCTCGA
TACCGCGCTCAGCCTTCTGGCGGCGCCTGCGGTCTTCCTGCCGGCGATTCTCAGCCTGCAGCGCGGTGAAAGACCGATCG
CGCCCGACGCTTCGCTGCCGCAAGCTGCCGATATCCTGCGCATGTTGACCGGACAATTGCCGAGCAAGGAGCAGACGGCC
GCGCTCGACGCCTACCTCGTGACCATTTCGGATCACGGCCTCAACGCCTCGACCTTCGCATCGCGCGTCATTGCCTCGAC
GCAGGCCGGCCTCACCTCTTCGGTCCTGGCCGCGCTGAGCGCCCTGAAGGGACCGCTGCATGGCGGTGCGCCCGGCCCGG
TGCTCGACATGCTGGATGCCGTGGGAACGGCAGAGAATGCCGGCCAATGGCTCAGCGAAGCGCTCGATCGCGGCGAAAGA
CTGATGGGTTTCGGCCACCGCATCTACCGCGTCCGCGATCCGCGCGCCGATGCGCTGAAGGGCGCTCTGAAGCCGCTGAT
ATCAACCGGGCAGGTCGACCCCGCCCGCGGCACGCTGGCGCAAGCCGTGGAAGCCGCCGCATTGGCAATCCTGAAGGCGC
GCAAGCCGAACCGGCCGCTCGACGTCAACGTCGAATTCTACACGGCGCTGCTGCTCGAAGCGCTCGGCTTCCCGCGGGAG
GCCTTCACCGGCGTCTTTGCAATCGGCCGCACTGTCGGGTGGCTGGCGCATGCCCGCGAACAGGCGCTCGACGGCCGGCT
GATTCGCCCGCGCTCGGTCTATATCGGCCCGCTGCCGGCCGCAGCCTGA

Upstream 100 bases:

>100_bases
GCGGTCAGCCACGGCATCGGAGCCTCATATTGATCAATAGCATCAAGATTGACGTCAATGAAACTAGGCCGGATCATCCG
GGCGGTAAAGGAGAAACAAT

Downstream 100 bases:

>100_bases
CGATCGAATTCGGCGGCGGGGCATGCTCCTGCCGCCCAGGCTTCAACCGCCGACGAAAGCGGCGAGGTTGTCGAGCGTCA
AACGGCAGCCTTCCTCATTG

Product: citrate synthase 2

Products: NA

Alternate protein names: Citrate synthase I [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKNGLEDVIAAETQLSDVDGEAGRLIIRGVSLDHLVADCSYEGVAALLLDGLIERSFDQAQLRAWLANARTRIFHHIGAA
DAALLTLPPVDAMRALIARLPDGEDLDTALSLLAAPAVFLPAILSLQRGERPIAPDASLPQAADILRMLTGQLPSKEQTA
ALDAYLVTISDHGLNASTFASRVIASTQAGLTSSVLAALSALKGPLHGGAPGPVLDMLDAVGTAENAGQWLSEALDRGER
LMGFGHRIYRVRDPRADALKGALKPLISTGQVDPARGTLAQAVEAAALAILKARKPNRPLDVNVEFYTALLLEALGFPRE
AFTGVFAIGRTVGWLAHAREQALDGRLIRPRSVYIGPLPAAA

Sequences:

>Translated_362_residues
MKNGLEDVIAAETQLSDVDGEAGRLIIRGVSLDHLVADCSYEGVAALLLDGLIERSFDQAQLRAWLANARTRIFHHIGAA
DAALLTLPPVDAMRALIARLPDGEDLDTALSLLAAPAVFLPAILSLQRGERPIAPDASLPQAADILRMLTGQLPSKEQTA
ALDAYLVTISDHGLNASTFASRVIASTQAGLTSSVLAALSALKGPLHGGAPGPVLDMLDAVGTAENAGQWLSEALDRGER
LMGFGHRIYRVRDPRADALKGALKPLISTGQVDPARGTLAQAVEAAALAILKARKPNRPLDVNVEFYTALLLEALGFPRE
AFTGVFAIGRTVGWLAHAREQALDGRLIRPRSVYIGPLPAAA
>Mature_362_residues
MKNGLEDVIAAETQLSDVDGEAGRLIIRGVSLDHLVADCSYEGVAALLLDGLIERSFDQAQLRAWLANARTRIFHHIGAA
DAALLTLPPVDAMRALIARLPDGEDLDTALSLLAAPAVFLPAILSLQRGERPIAPDASLPQAADILRMLTGQLPSKEQTA
ALDAYLVTISDHGLNASTFASRVIASTQAGLTSSVLAALSALKGPLHGGAPGPVLDMLDAVGTAENAGQWLSEALDRGER
LMGFGHRIYRVRDPRADALKGALKPLISTGQVDPARGTLAQAVEAAALAILKARKPNRPLDVNVEFYTALLLEALGFPRE
AFTGVFAIGRTVGWLAHAREQALDGRLIRPRSVYIGPLPAAA

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786939, Length=374, Percent_Identity=28.0748663101604, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1786527, Length=378, Percent_Identity=30.952380952381, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17555174, Length=200, Percent_Identity=30.5, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 38187; Mature: 38187

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNGLEDVIAAETQLSDVDGEAGRLIIRGVSLDHLVADCSYEGVAALLLDGLIERSFDQA
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHCCHHH
QLRAWLANARTRIFHHIGAADAALLTLPPVDAMRALIARLPDGEDLDTALSLLAAPAVFL
HHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
PAILSLQRGERPIAPDASLPQAADILRMLTGQLPSKEQTAALDAYLVTISDHGLNASTFA
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHEEEEEEECCCCCHHHHH
SRVIASTQAGLTSSVLAALSALKGPLHGGAPGPVLDMLDAVGTAENAGQWLSEALDRGER
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LMGFGHRIYRVRDPRADALKGALKPLISTGQVDPARGTLAQAVEAAALAILKARKPNRPL
HHHCCHHEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
DVNVEFYTALLLEALGFPREAFTGVFAIGRTVGWLAHAREQALDGRLIRPRSVYIGPLPA
EECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEECCCCC
AA
CC
>Mature Secondary Structure
MKNGLEDVIAAETQLSDVDGEAGRLIIRGVSLDHLVADCSYEGVAALLLDGLIERSFDQA
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHCCHHH
QLRAWLANARTRIFHHIGAADAALLTLPPVDAMRALIARLPDGEDLDTALSLLAAPAVFL
HHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
PAILSLQRGERPIAPDASLPQAADILRMLTGQLPSKEQTAALDAYLVTISDHGLNASTFA
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHEEEEEEECCCCCHHHHH
SRVIASTQAGLTSSVLAALSALKGPLHGGAPGPVLDMLDAVGTAENAGQWLSEALDRGER
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LMGFGHRIYRVRDPRADALKGALKPLISTGQVDPARGTLAQAVEAAALAILKARKPNRPL
HHHCCHHEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
DVNVEFYTALLLEALGFPREAFTGVFAIGRTVGWLAHAREQALDGRLIRPRSVYIGPLPA
EECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEECCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377 [H]