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The map label for this gene is gcvA [H]

Identifier: 209549379

GI number: 209549379

Start: 1827748

End: 1828653

Strand: Direct

Name: gcvA [H]

Synonym: Rleg2_1784

Alternate gene names: 209549379

Gene position: 1827748-1828653 (Clockwise)

Preceding gene: 209549374

Following gene: 209549386

Centisome position: 40.28

GC content: 63.47

Gene sequence:

>906_bases
ATGAGAGACCTGACCAGATTGAAGTCCCTGCAGGCGCTGGAGGCTTCCGCGAGACATGGCAGCTTTGTCGGAGCGGCAGC
CGAGCTGGATGTCACGCCTCCTGCCGTCGGGCAGCTCGTCCGTTCGCTGGAGGATTGGGTCGGCTATCCGCTGCTCAGAA
GAAGCCGTTCCGGCACGGAAAGGCTGACGCCCGTCGACGAGGCCCAGTATGCGCTCAATGATATCGCTGAAGGGCTCGAT
CGTCTCGAGACCGGGCTGCGGAAACTTCGCGGCAGGAAAGCCCGGTCGGTCGTGGTGGTCACCGCGTCCCAGGCGCTGGT
TGCCAACTGGCTGCTGGCAAGATTGGACGACTTCTCGACGAAACACCCGACGATCGATGTCCGGCTCGATGTCTCCGACA
GGGTCATCGATCTGGCCCAGGGCGAGGCCGATATCGGCATTCGCTGCGGTCTTGGTGCCTGGAAGGGTGTGAAATCGACA
TTCCTCATGAGCGAAGAGATTATCGCGGTTTGCCACAATCTGTTGCTCCAGGCGGGTCGGGAGGTGACCGCCGGCTGGAT
CGTCGAACAGACGCTGATCCATGACGGCACGCCGCATCCGGGCGGCGACTTTCCGACGTGGGCGGAATGGCTGGCCAAAT
CCGGCGCAAACCAGGTTCCGGCGGAACGCGGGCTGAAGATCAATTCCACGGCGGCAGTGATCCAGGCTGCCGTCGCGGCG
AGGGGCGTCGCCCTCGTGCGCAAGGCGCTCGTGGCACAGGAACTCGACAGCGGCAGGCTGGTCCATCTGTTGCCGGATGT
CCGCTGGCCTGTGAAGTGGGCGTATTACATCGTCGCAGCGCCAAAGGCGTTGCGTCGATACGAGGTTGCAGCCTTTCACG
ACTGGCTGGCCTCGCGCCCGAGATAG

Upstream 100 bases:

>100_bases
CGCAAACCTCTTTGTCTTGCCTCCAGGGTAGCGCGCAAGATTGCATTGGCGAGGAAAACCGGGCATACCGCAAAGAAAGA
AAATCTTTGTCGTGAGTGAG

Downstream 100 bases:

>100_bases
ACGCCGAGGAATAATCCCCGAACGAAAGACTACTCGCTTCAAACAGCCATATTCGACGCTGCCGACATCACTTCCCGAGG
GGCCGGACCACCGAACATAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRDLTRLKSLQALEASARHGSFVGAAAELDVTPPAVGQLVRSLEDWVGYPLLRRSRSGTERLTPVDEAQYALNDIAEGLD
RLETGLRKLRGRKARSVVVVTASQALVANWLLARLDDFSTKHPTIDVRLDVSDRVIDLAQGEADIGIRCGLGAWKGVKST
FLMSEEIIAVCHNLLLQAGREVTAGWIVEQTLIHDGTPHPGGDFPTWAEWLAKSGANQVPAERGLKINSTAAVIQAAVAA
RGVALVRKALVAQELDSGRLVHLLPDVRWPVKWAYYIVAAPKALRRYEVAAFHDWLASRPR

Sequences:

>Translated_301_residues
MRDLTRLKSLQALEASARHGSFVGAAAELDVTPPAVGQLVRSLEDWVGYPLLRRSRSGTERLTPVDEAQYALNDIAEGLD
RLETGLRKLRGRKARSVVVVTASQALVANWLLARLDDFSTKHPTIDVRLDVSDRVIDLAQGEADIGIRCGLGAWKGVKST
FLMSEEIIAVCHNLLLQAGREVTAGWIVEQTLIHDGTPHPGGDFPTWAEWLAKSGANQVPAERGLKINSTAAVIQAAVAA
RGVALVRKALVAQELDSGRLVHLLPDVRWPVKWAYYIVAAPKALRRYEVAAFHDWLASRPR
>Mature_301_residues
MRDLTRLKSLQALEASARHGSFVGAAAELDVTPPAVGQLVRSLEDWVGYPLLRRSRSGTERLTPVDEAQYALNDIAEGLD
RLETGLRKLRGRKARSVVVVTASQALVANWLLARLDDFSTKHPTIDVRLDVSDRVIDLAQGEADIGIRCGLGAWKGVKST
FLMSEEIIAVCHNLLLQAGREVTAGWIVEQTLIHDGTPHPGGDFPTWAEWLAKSGANQVPAERGLKINSTAAVIQAAVAA
RGVALVRKALVAQELDSGRLVHLLPDVRWPVKWAYYIVAAPKALRRYEVAAFHDWLASRPR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=33.1103678929766, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=27.7027027027027, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=29.0102389078498, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1786401, Length=283, Percent_Identity=23.6749116607774, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081978, Length=288, Percent_Identity=25.3472222222222, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32951; Mature: 32951

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50931 HTH_LYSR ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLTRLKSLQALEASARHGSFVGAAAELDVTPPAVGQLVRSLEDWVGYPLLRRSRSGTE
CCHHHHHHHHHHHHHHHCCCCEECCEEECCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCC
RLTPVDEAQYALNDIAEGLDRLETGLRKLRGRKARSVVVVTASQALVANWLLARLDDFST
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCC
KHPTIDVRLDVSDRVIDLAQGEADIGIRCGLGAWKGVKSTFLMSEEIIAVCHNLLLQAGR
CCCEEEEEECCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVTAGWIVEQTLIHDGTPHPGGDFPTWAEWLAKSGANQVPAERGLKINSTAAVIQAAVAA
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHCCCEECHHHHHHHHHHHH
RGVALVRKALVAQELDSGRLVHLLPDVRWPVKWAYYIVAAPKALRRYEVAAFHDWLASRP
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHEEECHHHHHHHHHHHHHHHHHCCC
R
C
>Mature Secondary Structure
MRDLTRLKSLQALEASARHGSFVGAAAELDVTPPAVGQLVRSLEDWVGYPLLRRSRSGTE
CCHHHHHHHHHHHHHHHCCCCEECCEEECCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCC
RLTPVDEAQYALNDIAEGLDRLETGLRKLRGRKARSVVVVTASQALVANWLLARLDDFST
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCC
KHPTIDVRLDVSDRVIDLAQGEADIGIRCGLGAWKGVKSTFLMSEEIIAVCHNLLLQAGR
CCCEEEEEECCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVTAGWIVEQTLIHDGTPHPGGDFPTWAEWLAKSGANQVPAERGLKINSTAAVIQAAVAA
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCHHCCCEECHHHHHHHHHHHH
RGVALVRKALVAQELDSGRLVHLLPDVRWPVKWAYYIVAAPKALRRYEVAAFHDWLASRP
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHEEECHHHHHHHHHHHHHHHHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]