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The map label for this gene is ntrX [H]

Identifier: 209549229

GI number: 209549229

Start: 1660176

End: 1661540

Strand: Direct

Name: ntrX [H]

Synonym: Rleg2_1630

Alternate gene names: 209549229

Gene position: 1660176-1661540 (Clockwise)

Preceding gene: 209549228

Following gene: 209549230

Centisome position: 36.58

GC content: 63.44

Gene sequence:

>1365_bases
ATGGCCTCTGATATTCTCGTCGTCGACGACGAGCACGATATTCGCGAGATCGTTTCCGGCATTCTCTCCGACGAGGGACA
CGAAACGCGCACGGCCCATGACAGCGACAGTGCGCTGGCGGCGATCTCCGATCGTGTGCCGCGGCTGATCTTCCTCGATA
TCTGGATGCAGGGCAGCAAGCTCGATGGTCTGTCGCTGCTCGACGAGATCAAGACCCGCCACCCGGATCTGCCCGTCGTG
ATGATCTCGGGCCATGGCAATATCGAAACCGCCGTCTCGGCGATCAAGCGCGGCGCCTTCGATTTTATCGAAAAGCCTTT
CAAGGCCGACCGGCTGATCCTGATCGCCGAACGGGCGCTGGAGAATTCCAAGCTGAAGCGCGAGGTCTCCGAGCTGAAGC
GGCGCACCGGCGACGCCCTGGAGCTGATCGGCACCTCGGTCGCCGTGTCGCAGTTGCGCCAGACGATCGAGAAGGTTTCG
CCGACCAACAGCCGCATCATGATCCTCGGCGCATCCGGCTCCGGCAAGGAGCTGGTGGCGCGGATGATCCACAAGAAGTC
GACCCGCGCCAACGGTCCCTTCGTGGCGATCAACGCTGCCAACATCACGCCCGATCGCATGGAAGTGGCGCTGTTCGGCA
CCGAGGGCACGCCCGGCCAGGCGCGCAAGATCGGCGCGCTCGAGGAAGCCCATCGCGGCATCCTCTATCTCGACGAAGTC
GGCGAGATGCCGCGCGAGACGCAGAACAAGATCCTGCGCGTGCTGGTCGATCAGCAGTTCGAGCGCGTCGGCGGCTCCAA
GCGCGTCAAGGTCGACGTCCGCATCATCTCCTCGACCGCCTATAATCTCGAAAGCCGCATCACCGAGGGATGGTTCCGAG
AGGATCTCTATCATCGCCTCGCCGTCGTGCCGGTGCGCGTGCCGGCGCTGGCCGAGCGGCGCGAGGATATTCCCTTCCTC
GTCGATCAGCTGATGCGGCAGATTTCCGAGCAGGCCGGCATTCGTCCGCGCCGCATCGGCGACGACGCCATGGCGGTGCT
GCAGGCGCATGATTGGCCGGGCAATATCCGCCAGCTGCGCAACAATATCGAGCGACTGATGATCCTTGCCCGCACCGACG
GACCGGATGCACCGATCACCGCGGACATGCTGCCGACCGATCTCGGCGACATGCTGCCGAAGGTCTCGGCCAAGAACGAC
TATCACATCATGACGCTGCCGCTGCGCGAAGCCCGCGAGATGTTCGAGAAGGATTATCTGATCGCCCAGATCAACCGCTT
CGGCGGCAATATCTCGCGCACCGCCGAATTCGTCGGCATGGAGCGTTCGGCGCTGCACCGCAAGCTGAAGTCGCTCGGCG
TCTAG

Upstream 100 bases:

>100_bases
GCCGATTTTGACCGGGGAAGAGGGGCCATGATCCGCGTGCATCTGCCACGCCGGGATCCGACGCCCACCTCTCCCGCAGC
CAATGACAAGGAAAGCGTTT

Downstream 100 bases:

>100_bases
AGCATGATGCCGAAAACTGTCAGCGGTTTTCGGGCAACATCATGCTCCACTTTTTGATCTGCCAGGCGCCATTGCAGTGC
CGCATTCCGTTATCGCTTTC

Product: Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MASDILVVDDEHDIREIVSGILSDEGHETRTAHDSDSALAAISDRVPRLIFLDIWMQGSKLDGLSLLDEIKTRHPDLPVV
MISGHGNIETAVSAIKRGAFDFIEKPFKADRLILIAERALENSKLKREVSELKRRTGDALELIGTSVAVSQLRQTIEKVS
PTNSRIMILGASGSGKELVARMIHKKSTRANGPFVAINAANITPDRMEVALFGTEGTPGQARKIGALEEAHRGILYLDEV
GEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLESRITEGWFREDLYHRLAVVPVRVPALAERREDIPFL
VDQLMRQISEQAGIRPRRIGDDAMAVLQAHDWPGNIRQLRNNIERLMILARTDGPDAPITADMLPTDLGDMLPKVSAKND
YHIMTLPLREAREMFEKDYLIAQINRFGGNISRTAEFVGMERSALHRKLKSLGV

Sequences:

>Translated_454_residues
MASDILVVDDEHDIREIVSGILSDEGHETRTAHDSDSALAAISDRVPRLIFLDIWMQGSKLDGLSLLDEIKTRHPDLPVV
MISGHGNIETAVSAIKRGAFDFIEKPFKADRLILIAERALENSKLKREVSELKRRTGDALELIGTSVAVSQLRQTIEKVS
PTNSRIMILGASGSGKELVARMIHKKSTRANGPFVAINAANITPDRMEVALFGTEGTPGQARKIGALEEAHRGILYLDEV
GEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLESRITEGWFREDLYHRLAVVPVRVPALAERREDIPFL
VDQLMRQISEQAGIRPRRIGDDAMAVLQAHDWPGNIRQLRNNIERLMILARTDGPDAPITADMLPTDLGDMLPKVSAKND
YHIMTLPLREAREMFEKDYLIAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
>Mature_453_residues
ASDILVVDDEHDIREIVSGILSDEGHETRTAHDSDSALAAISDRVPRLIFLDIWMQGSKLDGLSLLDEIKTRHPDLPVVM
ISGHGNIETAVSAIKRGAFDFIEKPFKADRLILIAERALENSKLKREVSELKRRTGDALELIGTSVAVSQLRQTIEKVSP
TNSRIMILGASGSGKELVARMIHKKSTRANGPFVAINAANITPDRMEVALFGTEGTPGQARKIGALEEAHRGILYLDEVG
EMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLESRITEGWFREDLYHRLAVVPVRVPALAERREDIPFLV
DQLMRQISEQAGIRPRRIGDDAMAVLQAHDWPGNIRQLRNNIERLMILARTDGPDAPITADMLPTDLGDMLPKVSAKNDY
HIMTLPLREAREMFEKDYLIAQINRFGGNISRTAEFVGMERSALHRKLKSLGV

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=36.9098712446352, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1788550, Length=461, Percent_Identity=36.0086767895879, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1790437, Length=449, Percent_Identity=34.2984409799555, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1788905, Length=449, Percent_Identity=33.184855233853, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1789087, Length=434, Percent_Identity=32.7188940092166, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789233, Length=231, Percent_Identity=41.991341991342, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786524, Length=329, Percent_Identity=34.0425531914894, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87082117, Length=320, Percent_Identity=33.4375, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI87082152, Length=326, Percent_Identity=34.0490797546012, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1787583, Length=319, Percent_Identity=33.2288401253918, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081872, Length=315, Percent_Identity=32.6984126984127, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI87081858, Length=289, Percent_Identity=28.3737024221453, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=31.0043668122271, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=38.4615384615385, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50705; Mature: 50574

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDILVVDDEHDIREIVSGILSDEGHETRTAHDSDSALAAISDRVPRLIFLDIWMQGSK
CCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCC
LDGLSLLDEIKTRHPDLPVVMISGHGNIETAVSAIKRGAFDFIEKPFKADRLILIAERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEEHHHH
ENSKLKREVSELKRRTGDALELIGTSVAVSQLRQTIEKVSPTNSRIMILGASGSGKELVA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
RMIHKKSTRANGPFVAINAANITPDRMEVALFGTEGTPGQARKIGALEEAHRGILYLDEV
HHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHCCHHHHCCCEEEEHHH
GEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLESRITEGWFREDLYHRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AVVPVRVPALAERREDIPFLVDQLMRQISEQAGIRPRRIGDDAMAVLQAHDWPGNIRQLR
HHEEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHH
NNIERLMILARTDGPDAPITADMLPTDLGDMLPKVSAKNDYHIMTLPLREAREMFEKDYL
HHHHHEEEEEECCCCCCCEECCCCCCCHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHH
IAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASDILVVDDEHDIREIVSGILSDEGHETRTAHDSDSALAAISDRVPRLIFLDIWMQGSK
CCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCC
LDGLSLLDEIKTRHPDLPVVMISGHGNIETAVSAIKRGAFDFIEKPFKADRLILIAERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCCCCEEEEEHHHH
ENSKLKREVSELKRRTGDALELIGTSVAVSQLRQTIEKVSPTNSRIMILGASGSGKELVA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
RMIHKKSTRANGPFVAINAANITPDRMEVALFGTEGTPGQARKIGALEEAHRGILYLDEV
HHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHHCCHHHHCCCEEEEHHH
GEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLESRITEGWFREDLYHRL
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AVVPVRVPALAERREDIPFLVDQLMRQISEQAGIRPRRIGDDAMAVLQAHDWPGNIRQLR
HHEEEECCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHH
NNIERLMILARTDGPDAPITADMLPTDLGDMLPKVSAKNDYHIMTLPLREAREMFEKDYL
HHHHHEEEEEECCCCCCCEECCCCCCCHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHH
IAQINRFGGNISRTAEFVGMERSALHRKLKSLGV
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]