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The map label for this gene is ntrC [H]

Identifier: 209549218

GI number: 209549218

Start: 1644436

End: 1645887

Strand: Direct

Name: ntrC [H]

Synonym: Rleg2_1619

Alternate gene names: 209549218

Gene position: 1644436-1645887 (Clockwise)

Preceding gene: 209549217

Following gene: 209549219

Centisome position: 36.24

GC content: 63.71

Gene sequence:

>1452_bases
ATGACAGCTACGATCCTCGTTGCAGATGATGATGCGGCCATCCGCACCGTGCTCAACCAGGCTTTGAGCCGCGCCGGCTA
TGACGTTCGCATCACCTCCAACGCCGCTACCCTCTGGCGCTGGATTTCGGCGGGTGAGGGCGATCTGGTCGTTACCGACG
TGGTGATGCCTGACGAAAACGCCTTCGACCTGCTGCCGCGCATCAAGAAGGCGCGTCCCGATCTGCCCGTCCTCGTCATG
AGCGCGCAGAACACCTTCATGACCGCCATCAAGGCCTCGGAGAAGGGCGCCTACGACTATCTGCCGAAGCCCTTCGATCT
CACCGAGCTGATCGGCATCATCGGCCGCGCACTCGCCGAGCCGAAGCGCAAGCCGGCCAAGCTCGAAGAGGACATGCAGG
ACGGCATGCCGCTGGTCGGCCGGTCGGCGGCGATGCAGGAAATCTACCGCGTGCTTGCCCGGCTGATGCAGACGGACCTG
ACGCTGATGATCACCGGCGAATCCGGTACCGGAAAGGAGCTCGTCGCCCGCGCGCTGCATGATTACGGCAAGCGCCGCAA
CGGCCCCTTCGTGGCGATCAACATGGCGGCGATCCCGCGCGACCTGATCGAATCGGAACTCTTCGGCCATGAGAAAGGCG
CCTTCACCGGTGCCCAGACCCGCTCGACCGGCCGCTTCGAGCAGGCCGAAGGCGGCACGCTGTTTCTAGACGAGATCGGC
GACATGCCGATGGACGCCCAGACGCGGCTTCTGCGCGTGCTGCAGCAGGGTGAATACACCACCGTCGGCGGCCGCACGCC
GATCCGCACCGACGTGCGCATCGTCGCCGCCACCAACAAGGATTTGAAACAGGCGATCAACCAGGGCCTCTTCCGCGAGG
ACCTCTATTATCGTCTCAACGTCGTGCCACTGCGCCTGCCGCCGCTGCGCGACCGCGCCGAGGATATTCCCGATCTCGTG
CGCCATTTCATCCAGCAGGCGGAGAAGGAGGGCCTCGGCTCCAAGCGCTTCGATCAGGAAGCGCTGGACCTGATGAAGGC
CTATGCCTGGCCGGGCAATGTCCGCGAGTTGGAAAACCTCATCCGTCGCCTGATGGCGCTCTATCCGCAGGATATCATCA
CCCGCGAAATCATCGACGCGGAGCTTCGCTCCGACGTGCCCGACAGCCCGATCGACAAGGGTCCGATGCGCAGCGGCTCG
ATGACGATCGCCCAGGCCGTCGAGGAGAACATGCGCACCTATTTCTCGGGCTTCGGCGAAAACCTGCCGCCGCCCGGACT
CTATGACCGCGTGCTGACGGAAATGGAATATCCGCTGATCCTCGCTGCGCTGACGGCAACGCGCGGCAACCAGATCAAGG
CAGCCGATCTTCTCGGCCTCAACCGCAACACCTTGCGCAAGAAGATCAGGGAACTGGGCGTCTCGGTTTACAGAAGCTCC
CGCACCGCCTGA

Upstream 100 bases:

>100_bases
ACAGCCAGAACAGCCGCACGACATTCCGCGTCCTCATGCCGGCGTCGAAGGACGCCTCGCTCGAAGACGCCTCTTCCGCA
AGCTCGACAGGACCTTCTCG

Downstream 100 bases:

>100_bases
ATAAAAACTGGCTGAATGAAAAGAGGGCGGCGGGCCCTCATGTTGCCCACCGCCCACTTCCTCCCGGCGTCCGCCGCCAC
GGTCGGGAAGAGAATTGAAT

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWISAGEGDLVVTDVVMPDENAFDLLPRIKKARPDLPVLVM
SAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRKPAKLEEDMQDGMPLVGRSAAMQEIYRVLARLMQTDL
TLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIG
DMPMDAQTRLLRVLQQGEYTTVGGRTPIRTDVRIVAATNKDLKQAINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLV
RHFIQQAEKEGLGSKRFDQEALDLMKAYAWPGNVRELENLIRRLMALYPQDIITREIIDAELRSDVPDSPIDKGPMRSGS
MTIAQAVEENMRTYFSGFGENLPPPGLYDRVLTEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSS
RTA

Sequences:

>Translated_483_residues
MTATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWISAGEGDLVVTDVVMPDENAFDLLPRIKKARPDLPVLVM
SAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRKPAKLEEDMQDGMPLVGRSAAMQEIYRVLARLMQTDL
TLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIG
DMPMDAQTRLLRVLQQGEYTTVGGRTPIRTDVRIVAATNKDLKQAINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLV
RHFIQQAEKEGLGSKRFDQEALDLMKAYAWPGNVRELENLIRRLMALYPQDIITREIIDAELRSDVPDSPIDKGPMRSGS
MTIAQAVEENMRTYFSGFGENLPPPGLYDRVLTEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSS
RTA
>Mature_482_residues
TATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWISAGEGDLVVTDVVMPDENAFDLLPRIKKARPDLPVLVMS
AQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKRKPAKLEEDMQDGMPLVGRSAAMQEIYRVLARLMQTDLT
LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIGD
MPMDAQTRLLRVLQQGEYTTVGGRTPIRTDVRIVAATNKDLKQAINQGLFREDLYYRLNVVPLRLPPLRDRAEDIPDLVR
HFIQQAEKEGLGSKRFDQEALDLMKAYAWPGNVRELENLIRRLMALYPQDIITREIIDAELRSDVPDSPIDKGPMRSGSM
TIAQAVEENMRTYFSGFGENLPPPGLYDRVLTEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSSR
TA

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=475, Percent_Identity=44.2105263157895, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1788550, Length=486, Percent_Identity=38.6831275720165, Blast_Score=327, Evalue=7e-91,
Organism=Escherichia coli, GI1790437, Length=466, Percent_Identity=41.2017167381974, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788905, Length=466, Percent_Identity=36.2660944206009, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1789233, Length=338, Percent_Identity=42.3076923076923, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=52.8138528138528, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1789087, Length=341, Percent_Identity=41.0557184750733, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=46.3203463203463, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87081872, Length=337, Percent_Identity=39.1691394658754, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1786524, Length=249, Percent_Identity=43.7751004016064, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1787583, Length=251, Percent_Identity=41.0358565737052, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789828, Length=231, Percent_Identity=36.7965367965368, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87081858, Length=323, Percent_Identity=29.7213622291022, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789809, Length=110, Percent_Identity=32.7272727272727, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786784, Length=107, Percent_Identity=30.8411214953271, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790552, Length=106, Percent_Identity=36.7924528301887, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53836; Mature: 53704

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWISAGEGDLVVTDVVMPDEN
CCEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCCC
AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE
HHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PKRKPAKLEEDMQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALH
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
DYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIG
HHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHC
DMPMDAQTRLLRVLQQGEYTTVGGRTPIRTDVRIVAATNKDLKQAINQGLFREDLYYRLN
CCCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCHHHHHHHHHCHHHHHHHEEEE
VVPLRLPPLRDRAEDIPDLVRHFIQQAEKEGLGSKRFDQEALDLMKAYAWPGNVRELENL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
IRRLMALYPQDIITREIIDAELRSDVPDSPIDKGPMRSGSMTIAQAVEENMRTYFSGFGE
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
NLPPPGLYDRVLTEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSS
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHC
RTA
CCC
>Mature Secondary Structure 
TATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWISAGEGDLVVTDVVMPDEN
CEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCCC
AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE
HHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PKRKPAKLEEDMQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALH
CCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
DYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFEQAEGGTLFLDEIG
HHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHC
DMPMDAQTRLLRVLQQGEYTTVGGRTPIRTDVRIVAATNKDLKQAINQGLFREDLYYRLN
CCCCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCHHHHHHHHHCHHHHHHHEEEE
VVPLRLPPLRDRAEDIPDLVRHFIQQAEKEGLGSKRFDQEALDLMKAYAWPGNVRELENL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
IRRLMALYPQDIITREIIDAELRSDVPDSPIDKGPMRSGSMTIAQAVEENMRTYFSGFGE
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
NLPPPGLYDRVLTEMEYPLILAALTATRGNQIKAADLLGLNRNTLRKKIRELGVSVYRSS
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHC
RTA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]