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The map label for this gene is yfbT [H]

Identifier: 209549052

GI number: 209549052

Start: 1472102

End: 1472782

Strand: Direct

Name: yfbT [H]

Synonym: Rleg2_1449

Alternate gene names: 209549052

Gene position: 1472102-1472782 (Clockwise)

Preceding gene: 209549051

Following gene: 209549059

Centisome position: 32.44

GC content: 62.7

Gene sequence:

>681_bases
TTGTCCCAACCGATGCCCCATGGCTTCGAGAAGCCTTATGCCGCCTTCCTGTTCGATATGGACGGCACAATCCTCAATTC
GATCCGTGCGGCCGAACGTGTCTGGAGCGACTGGGCAAGACGTCAGGGGCTCGATGTCGCCGACTTTCTGCCGAAGATGC
ATGGATCGCGCGGCGTCGACACGATCGCCCGGCTGAACCTGCCGGGCGTCGATCCCGAACATGAAGCCAGGCTGGTGACC
GAGGCCGAGATCGCCGATGTCGGCGATGTCGTCGCCATTTCGGGTGCCGCGGCATTCCTGAGTTCGCTGCCGCCGGATCG
CTGGGCGATCGTCACCTCCTCACCCTTGCGTCTTGCCCGCCGGCGGCTGGAGGCCGCAGGCCTGTCGCTGCCGAAATTCA
TGGTGACGGCTGAAGATGTGAAGGTCGGAAAACCCGATCCGCAATGTTATATTCTGGGCGCCGAACGCCTCGGCGTCAGC
ACGCAGGACTGCCTGGTGTTCGAGGATGTCGCTGCCGGCATTCTCGCCGGCGAGGCGGCGGGCGCCGATGTCATGGTGGT
GACGGCAACCCATCATGACAAGATGGAAACGCCGCATCCGACGCTGTCATCCTACGACGAGATCTCGGTCCGCATCTCGG
CCGACCATAAAATGTTCATCGTGCCGAAGGCGGCCGGATAA

Upstream 100 bases:

>100_bases
TAGCTGACCTGTCCAGGCGAAAGCTTGCAAAAGGCGATGATTTGCCGTTTCATCGGGCGATAGCGACCACCGTCCGACTC
TTTTCCTGAAGGATTCCGCC

Downstream 100 bases:

>100_bases
GTCCTGCCTTTCACTCCGCGGCGGCGGCAAGACAGCGATCGATGATCCTGCCGATTTCGCCGCGGCAGGAGCCGCAATTG
GTTCCGGCGCTCGTTTCCTT

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSQPMPHGFEKPYAAFLFDMDGTILNSIRAAERVWSDWARRQGLDVADFLPKMHGSRGVDTIARLNLPGVDPEHEARLVT
EAEIADVGDVVAISGAAAFLSSLPPDRWAIVTSSPLRLARRRLEAAGLSLPKFMVTAEDVKVGKPDPQCYILGAERLGVS
TQDCLVFEDVAAGILAGEAAGADVMVVTATHHDKMETPHPTLSSYDEISVRISADHKMFIVPKAAG

Sequences:

>Translated_226_residues
MSQPMPHGFEKPYAAFLFDMDGTILNSIRAAERVWSDWARRQGLDVADFLPKMHGSRGVDTIARLNLPGVDPEHEARLVT
EAEIADVGDVVAISGAAAFLSSLPPDRWAIVTSSPLRLARRRLEAAGLSLPKFMVTAEDVKVGKPDPQCYILGAERLGVS
TQDCLVFEDVAAGILAGEAAGADVMVVTATHHDKMETPHPTLSSYDEISVRISADHKMFIVPKAAG
>Mature_225_residues
SQPMPHGFEKPYAAFLFDMDGTILNSIRAAERVWSDWARRQGLDVADFLPKMHGSRGVDTIARLNLPGVDPEHEARLVTE
AEIADVGDVVAISGAAAFLSSLPPDRWAIVTSSPLRLARRRLEAAGLSLPKFMVTAEDVKVGKPDPQCYILGAERLGVST
QDCLVFEDVAAGILAGEAAGADVMVVTATHHDKMETPHPTLSSYDEISVRISADHKMFIVPKAAG

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI87082080, Length=179, Percent_Identity=43.5754189944134, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI1789046, Length=181, Percent_Identity=34.2541436464088, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790151, Length=203, Percent_Identity=26.6009852216749, Blast_Score=64, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6321833, Length=197, Percent_Identity=34.010152284264, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321834, Length=196, Percent_Identity=33.6734693877551, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320905, Length=166, Percent_Identity=33.1325301204819, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6322136, Length=168, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24359; Mature: 24228

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPMPHGFEKPYAAFLFDMDGTILNSIRAAERVWSDWARRQGLDVADFLPKMHGSRGVD
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
TIARLNLPGVDPEHEARLVTEAEIADVGDVVAISGAAAFLSSLPPDRWAIVTSSPLRLAR
HHEECCCCCCCCCCCCEEEEHHHHHCCCCEEEECHHHHHHHCCCCCCEEEEECCHHHHHH
RRLEAAGLSLPKFMVTAEDVKVGKPDPQCYILGAERLGVSTQDCLVFEDVAAGILAGEAA
HHHHHHCCCCCCEEEEECCEECCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHCCCC
GADVMVVTATHHDKMETPHPTLSSYDEISVRISADHKMFIVPKAAG
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCC
>Mature Secondary Structure 
SQPMPHGFEKPYAAFLFDMDGTILNSIRAAERVWSDWARRQGLDVADFLPKMHGSRGVD
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
TIARLNLPGVDPEHEARLVTEAEIADVGDVVAISGAAAFLSSLPPDRWAIVTSSPLRLAR
HHEECCCCCCCCCCCCEEEEHHHHHCCCCEEEECHHHHHHHCCCCCCEEEEECCHHHHHH
RRLEAAGLSLPKFMVTAEDVKVGKPDPQCYILGAERLGVSTQDCLVFEDVAAGILAGEAA
HHHHHHCCCCCCEEEEECCEECCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHCCCC
GADVMVVTATHHDKMETPHPTLSSYDEISVRISADHKMFIVPKAAG
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123 [H]