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The map label for this gene is xthA [H]

Identifier: 209548989

GI number: 209548989

Start: 1409664

End: 1410455

Strand: Reverse

Name: xthA [H]

Synonym: Rleg2_1386

Alternate gene names: 209548989

Gene position: 1410455-1409664 (Counterclockwise)

Preceding gene: 209548990

Following gene: 209548976

Centisome position: 31.08

GC content: 61.49

Gene sequence:

>792_bases
GTGAAAATCGCAACCTATAACGTCAACGGGATCAATGGACGGTTGGCCATCCTGTTGCGCTGGCTCAAGGAGGACGCCCC
TGATGTGGTCTGCCTCCAGGAACTGAAGACAGACGACGCCGGGTTTCCACGAAAAGAGATTGAACGATCCGGCTATGGCG
CCGTCTGGCACGGACAGAGATCCTGGAACGGGGTCGCCATATTGGCGAAGGACAAGACACCGATCCTGACTCGGCGCGGT
CTTCCCGGCGATCCCGATGACAGCCACAGCCGCTATATCGAAGCCGTTATCGACGGCATCGTGATCGGATGCCTCTATCT
GCCGAACGGCAATCCCTTCCCAGGCGCCAAGTTCGACTACAAGCTCCGCTGGTTCCGTCGCCTGCACGACTACGCCGCCG
AACTGCTGGCACTCGAAGTGCCGTCGATCCTGGCCGGCGACTTCAATGTCATGCCGACGGAGATCGACGTTTATAAGCCG
GAACGCTGGCTCGACGACGCGCTTTTCAGGCCGGAGGTTCGCAAGGCCTATGCCCATCTCGTCGGCCAGGGCTGGACCGA
CGCCGTCAGGCATTTGCATCCGCACGAGCGCATCTACACCTTCTGGAAATACTGGCGCAACAGCTTCGAGCGCGACGCGG
GGCTACGGATCGACCACTTCCTGCTCAGCCCGGCCGTCGCGCCATGGCTTCGATCGGCGACCGTCCGGAGAAAGCCGCGC
GGCTGGGAGCATACCAGCGACCATGCCCCCGTGATGATCGAACTCGACGTTCCCAGCGTTGCAGCCGTCTGA

Upstream 100 bases:

>100_bases
CCAGGACGTCGAAGACCGGATACGTCAGCAGCCGTTGACGGCGGTCGCGATCGTCGCCGGCATTGCCTTCATCTTCGGAG
CGACGCGTTGAGCGATCAGC

Downstream 100 bases:

>100_bases
GCCGCGATGGCGCTCATTCATCCGCGCCGAGATCATAGATGCGGCCTGTCAGCCAGGCCGGCCAGTCTCGCTCGAAGAAG
GATTTGGCGTTTTCATCCAA

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKIATYNVNGINGRLAILLRWLKEDAPDVVCLQELKTDDAGFPRKEIERSGYGAVWHGQRSWNGVAILAKDKTPILTRRG
LPGDPDDSHSRYIEAVIDGIVIGCLYLPNGNPFPGAKFDYKLRWFRRLHDYAAELLALEVPSILAGDFNVMPTEIDVYKP
ERWLDDALFRPEVRKAYAHLVGQGWTDAVRHLHPHERIYTFWKYWRNSFERDAGLRIDHFLLSPAVAPWLRSATVRRKPR
GWEHTSDHAPVMIELDVPSVAAV

Sequences:

>Translated_263_residues
MKIATYNVNGINGRLAILLRWLKEDAPDVVCLQELKTDDAGFPRKEIERSGYGAVWHGQRSWNGVAILAKDKTPILTRRG
LPGDPDDSHSRYIEAVIDGIVIGCLYLPNGNPFPGAKFDYKLRWFRRLHDYAAELLALEVPSILAGDFNVMPTEIDVYKP
ERWLDDALFRPEVRKAYAHLVGQGWTDAVRHLHPHERIYTFWKYWRNSFERDAGLRIDHFLLSPAVAPWLRSATVRRKPR
GWEHTSDHAPVMIELDVPSVAAV
>Mature_263_residues
MKIATYNVNGINGRLAILLRWLKEDAPDVVCLQELKTDDAGFPRKEIERSGYGAVWHGQRSWNGVAILAKDKTPILTRRG
LPGDPDDSHSRYIEAVIDGIVIGCLYLPNGNPFPGAKFDYKLRWFRRLHDYAAELLALEVPSILAGDFNVMPTEIDVYKP
ERWLDDALFRPEVRKAYAHLVGQGWTDAVRHLHPHERIYTFWKYWRNSFERDAGLRIDHFLLSPAVAPWLRSATVRRKPR
GWEHTSDHAPVMIELDVPSVAAV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=31.6793893129771, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=31.6793893129771, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=31.6793893129771, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=34.3396226415094, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71989536, Length=267, Percent_Identity=29.5880149812734, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221330655, Length=270, Percent_Identity=30.7407407407407, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=31.203007518797, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30130; Mature: 30130

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATYNVNGINGRLAILLRWLKEDAPDVVCLQELKTDDAGFPRKEIERSGYGAVWHGQR
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHHHHCCCCCEEEECCC
SWNGVAILAKDKTPILTRRGLPGDPDDSHSRYIEAVIDGIVIGCLYLPNGNPFPGAKFDY
CCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCH
KLRWFRRLHDYAAELLALEVPSILAGDFNVMPTEIDVYKPERWLDDALFRPEVRKAYAHL
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHH
VGQGWTDAVRHLHPHERIYTFWKYWRNSFERDAGLRIDHFLLSPAVAPWLRSATVRRKPR
HCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GWEHTSDHAPVMIELDVPSVAAV
CCCCCCCCCCEEEEECCCHHCCC
>Mature Secondary Structure
MKIATYNVNGINGRLAILLRWLKEDAPDVVCLQELKTDDAGFPRKEIERSGYGAVWHGQR
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHHHHCCCCCEEEECCC
SWNGVAILAKDKTPILTRRGLPGDPDDSHSRYIEAVIDGIVIGCLYLPNGNPFPGAKFDY
CCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCH
KLRWFRRLHDYAAELLALEVPSILAGDFNVMPTEIDVYKPERWLDDALFRPEVRKAYAHL
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHH
VGQGWTDAVRHLHPHERIYTFWKYWRNSFERDAGLRIDHFLLSPAVAPWLRSATVRRKPR
HCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GWEHTSDHAPVMIELDVPSVAAV
CCCCCCCCCCEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]