BacMap

Definition	Rhizobium leguminosarum bv. trifolii WSM2304 chromosome, complete genome.
Accession	NC_011369
Length	4,537,948

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The map label for this gene is paaF [C]

Identifier: 209548979

GI number: 209548979

Start: 1396354

End: 1397133

Strand: Direct

Name: paaF [C]

Synonym: Rleg2_1376

Alternate gene names: 209548979

Gene position: 1396354-1397133 (Clockwise)

Preceding gene: 209548978

Following gene: 209548980

Centisome position: 30.77

GC content: 65.51

Gene sequence:

>780_bases
ATGGCTAGCGGAACAGTCAGGATCGACATTGACGGACATGTTGCGACCTTGACGATTTCTCGTCCGGAGAAGCTGAACGC
GCTCGATCTCGACATGCTGAAAGCCTTGGCGGATGCCGCTGACGTAGTCGAGGCGAACCCGGACGTGCGCGCTGCCGTTC
TGACGGGCGAAGGAAAGGGTTTTTCCGCCGGAGGCGATATCAAGGCCTGGGGCGGAATGCTGCCGCAGGAGTTCGGCCAT
GCCTGGGTTCGACACGGTCACCGGATCTTCGAACGGCTGGCGACGCTCCGGGTGCCGCTGGTCGCGGCCCTGAACGGCCA
CGCCCTCGGCGGAGGGCTCGAACTGGCAGGCGTTGCCGACATTCGCATCGCAGAGGAGCACATTAAGATCGGCCTGCCGG
AGACCGGCCTTGGAATGGTGCCGGGCTGGTCCGGCACCCAGCGTCTCGTGCGCCGTTTCGGCGCGCAGGCCGTCCGGCGC
ATGGCGCTCGGCGGCGAGATCTTCACCGCCGAGGAGGCGCGCCTGCTCGGGATCGTCGATGCGGTCGTTCCCTCGGGAGG
CGCGCTGGCTGCCGCCCGGGATTATGCGCAGCGGATTGCCGCAAGGGGGCCAGCTGCTGTCGAGATCGCCAAGCTGATGA
TCGCATCGGCAAATGGTGAGGACAACGGAACCGCGGTCGAAGCCCTGGGCTCGATCCTCGCTGCCAAGACCGGCGATCTG
AAAGAGGGCGTCGCATCGTTCAGCGAGAAGCGCCCGGCAACGTTCAAGGGAGAATGGTAA

Upstream 100 bases:

>100_bases
GCGCCTGACCTGAAGGTCATGGATGCGGCACTCTTCGGCGAGGCCGGCATCGGCCTGTCGCTTCCAGCCAAAAGCGCGCG
GACGCTGGAGGGTACTTTCC

Downstream 100 bases:

>100_bases
TGACGGTCCTCGTCAACCCCACGGCGCTCAGCGACCACAAGGCGCGTGATTTCAAGATGCTGATCGACGGCAGATGGGAG
ACCGGGGCCGCCGATCCGAT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MASGTVRIDIDGHVATLTISRPEKLNALDLDMLKALADAADVVEANPDVRAAVLTGEGKGFSAGGDIKAWGGMLPQEFGH
AWVRHGHRIFERLATLRVPLVAALNGHALGGGLELAGVADIRIAEEHIKIGLPETGLGMVPGWSGTQRLVRRFGAQAVRR
MALGGEIFTAEEARLLGIVDAVVPSGGALAAARDYAQRIAARGPAAVEIAKLMIASANGEDNGTAVEALGSILAAKTGDL
KEGVASFSEKRPATFKGEW

Sequences:

>Translated_259_residues
MASGTVRIDIDGHVATLTISRPEKLNALDLDMLKALADAADVVEANPDVRAAVLTGEGKGFSAGGDIKAWGGMLPQEFGH
AWVRHGHRIFERLATLRVPLVAALNGHALGGGLELAGVADIRIAEEHIKIGLPETGLGMVPGWSGTQRLVRRFGAQAVRR
MALGGEIFTAEEARLLGIVDAVVPSGGALAAARDYAQRIAARGPAAVEIAKLMIASANGEDNGTAVEALGSILAAKTGDL
KEGVASFSEKRPATFKGEW
>Mature_258_residues
ASGTVRIDIDGHVATLTISRPEKLNALDLDMLKALADAADVVEANPDVRAAVLTGEGKGFSAGGDIKAWGGMLPQEFGHA
WVRHGHRIFERLATLRVPLVAALNGHALGGGLELAGVADIRIAEEHIKIGLPETGLGMVPGWSGTQRLVRRFGAQAVRRM
ALGGEIFTAEEARLLGIVDAVVPSGGALAAARDYAQRIAARGPAAVEIAKLMIASANGEDNGTAVEALGSILAAKTGDLK
EGVASFSEKRPATFKGEW

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=35.15625, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=34.9206349206349, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=35.3932584269663, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI68989263, Length=202, Percent_Identity=33.6633663366337, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=25.296442687747, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI213417737, Length=211, Percent_Identity=26.0663507109005, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI157694516, Length=211, Percent_Identity=26.0663507109005, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI62530384, Length=174, Percent_Identity=32.183908045977, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI37594469, Length=153, Percent_Identity=28.7581699346405, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594471, Length=153, Percent_Identity=28.7581699346405, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI150378541, Length=255, Percent_Identity=27.0588235294118, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI261878539, Length=115, Percent_Identity=39.1304347826087, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=34.8178137651822, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=34.6153846153846, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=32.3308270676692, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=34.3434343434343, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=32.9411764705882, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=27.3809523809524, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082183, Length=270, Percent_Identity=25.1851851851852, Blast_Score=67, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=34.9397590361446, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=34.4398340248963, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=217, Percent_Identity=32.7188940092166, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=241, Percent_Identity=29.4605809128631, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=225, Percent_Identity=27.5555555555556, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=227, Percent_Identity=33.0396475770925, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=227, Percent_Identity=33.0396475770925, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=227, Percent_Identity=33.0396475770925, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=23.828125, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=198, Percent_Identity=30.3030303030303, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=216, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=227, Percent_Identity=23.7885462555066, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=244, Percent_Identity=24.5901639344262, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=179, Percent_Identity=26.8156424581006, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20129971, Length=253, Percent_Identity=35.1778656126482, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653477, Length=253, Percent_Identity=35.1778656126482, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=28.90625, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24650670, Length=250, Percent_Identity=29.6, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920382, Length=206, Percent_Identity=29.126213592233, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357171, Length=197, Percent_Identity=28.9340101522843, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583165, Length=181, Percent_Identity=33.7016574585635, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583077, Length=202, Percent_Identity=36.6336633663366, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583079, Length=202, Percent_Identity=36.6336633663366, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921000, Length=202, Percent_Identity=36.6336633663366, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921018, Length=221, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571730, Length=136, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28571729, Length=136, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26923; Mature: 26792

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGTVRIDIDGHVATLTISRPEKLNALDLDMLKALADAADVVEANPDVRAAVLTGEGKG
CCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
FSAGGDIKAWGGMLPQEFGHAWVRHGHRIFERLATLRVPLVAALNGHALGGGLELAGVAD
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEE
IRIAEEHIKIGLPETGLGMVPGWSGTQRLVRRFGAQAVRRMALGGEIFTAEEARLLGIVD
EEEEHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHH
AVVPSGGALAAARDYAQRIAARGPAAVEIAKLMIASANGEDNGTAVEALGSILAAKTGDL
HHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCH
KEGVASFSEKRPATFKGEW
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ASGTVRIDIDGHVATLTISRPEKLNALDLDMLKALADAADVVEANPDVRAAVLTGEGKG
CCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
FSAGGDIKAWGGMLPQEFGHAWVRHGHRIFERLATLRVPLVAALNGHALGGGLELAGVAD
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEE
IRIAEEHIKIGLPETGLGMVPGWSGTQRLVRRFGAQAVRRMALGGEIFTAEEARLLGIVD
EEEEHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHH
AVVPSGGALAAARDYAQRIAARGPAAVEIAKLMIASANGEDNGTAVEALGSILAAKTGDL
HHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCH
KEGVASFSEKRPATFKGEW
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA